The Kirby-Bauer Method for Antibiotic Susceptibility (with examples)
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- เผยแพร่เมื่อ 13 เม.ย. 2019
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Thanks for the comment!
Thanks a lot Really i got lot af adventage about this lesson and i would like to get Report of B+ bacteria culure and also N- bacteria culture
Tq.. Sir nice teach
Thank you!!
Glad you liked this video!
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nice!!
Regarding the procedure for the antibiotic susceptibility test, would it still work if a spread plate technique was done opposed to using a swap to cover the HE plate?
Good vid.but from where do the numbers that are used as a criteria come from?
Where did you find the data on the diameters of antibiotics to classify susceptibility? What source or website?
Where's your link of the past video please hehe
Thx alot
No problem!
If zones of diameter are the same size which antibiotic would you choose as the best one?
So if there is no treatment on the plate but there is a ZOI, would that make it invalid or?
I have a question if we found that all the antibiotic give susceptible result, which one will be the best antibiotic that can be prescribed to the patient?
May I ask you how to access CLSI on AST?
Is it possible to do this method with unknown bacteria? Let's say you go out and collect bacteria from a park bench, then plate the bacteria, isolate individual colonies, and then expose each colony to this method? My concern would be you could test the unknown bacteria against an antibiotic that does not kill that type of bacteria and then call it resistant. Any suggestions?
Great question!. I’m glad to see that I’m not the only one who inquired about the exact instance.
First and foremost, it is not a good idea to collect unknown bacteria from a park bench or any surroundings as you could easily end up collecting virulent pathogenic strains which would be a serious biohazard for all experimenters. Nevermind, proceeding with the unknown bacteria idea which is a nice idea you have ,let's answer the remainder of your question. So let's say we have isolated an unknown bacteria which is non-pathogenic to Homo sapiens and exposed it to the antibiotic using the method discussed above. We may find that there is no zone of inhibition and thus we would say that the antibiotic is "ineffective" against this unknown bacteria. Antibiotics whether both lytic or static ,have targets in the bacterial cell and the absence of these targets renders them powerless to affect a bacterial cell. So your unknown bacteria would simply not have the target used by the respective antibiotic to affect it. Resistance on the other hand is when you have a known bacteria which was always being inhibited by the antibiotic and then after some time, you find a colony that grows uninhibited . This would be termed as "resistance" . We would say that the members of the colony gained " resistance" against that antibiotic by mutation.
Kirby was the scietific? I can't find photos of his face.
In real life, the zone of inhibition is distorted and not a perfect circle as illustrated here. How would the diameter be calculated then?
im here just by curiosity i have just. learn i dint even take this class lol