Liquid Culture Basics
ฝัง
- เผยแพร่เมื่อ 6 มิ.ย. 2024
- Today's class we will be going over liquid culture. I'll go over what it is, and how you can make some of your own.
⬙ PGT Links ⬙
🧫Links to all products/gear I use: kit.co/PhillyGoldenTeacher
(Using these links will help sensei earn a small commission and support the channel)
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🌱BoomerShroomer Inflatable Tubs
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PGT Ultimate Lid Tek Video (how to create your own liquid culture lids)
• The Ultimate Lid Tek G...
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PGT is single handedly colonizing mushroom growers. Status ... legend
😄
I just complete my first grow of B+ thanks to your broke boi tek guide! I am totally obsessed with mycology and I appreciate all of your videos! This hobby is a gift that just keeps giving!
How are the B+ doing for you? I just ordered some prints and have heard great things. Only have experienced the Tidal Wave and Golden Teachers to date. Both great!
@@TopherHess1974 B+ is nice. But I prefer the teachers. F+ is really nice as well
@ intellectual crackhead I am bout to grow some b+ aswell I have a good syringe of them. Did the video method work good for b+?
@@masonreed5684 it doesn’t matter which variety you grow of Cubensis. The process is exactly the same…exactly! A cube is a cube. The only difference are PE’s, and that’s when you spawn them to bulk because you need to case them or you get blobs and mutations. But the LC and agar process is the same. PE (penis envy) take longer to colonize as well.
B+ were my first grow! Super fun and yields great 👍
The Presto PC is nice man. I want to thank you for all the tuts and all the knowledge. I’ve been doing this for about a year now and I’ve made quite a few adjustments based on your teachings. I strictly only grow APE, PE uncut and golden teacher now. Not a day goes by where I’m not working on Mycology. 🙏
I have the 28 qt Presto and It works very well. I just started doing LC's and for 1 or 2 quart jars, I use the instapot. Wife is not happy about that though. I'm going to start using 1/2 pint wide mouth jars for LC's rather than Qts.
Just tried LC using his technique. I am tickled pink it is 3 days old and showing lots of life. THANKS Teach.
Class is in session. Finally it’s here Couldn’t have came at a better time was thinking about starting some!!! Good looking out bro bro. 🔥🙏🙏🙏
One of my favorite channels.
Thanks for the info, and keep fighting the good fight.
I make my own maple syrup, I use that in my agar and liquid culture. They seem to love it and stays very clear.
Tell me u r Canadian without telling me u r Canadian: 😂
Organik bal da olur mu?
Love your videos. Simple, easy, loaded with information. Thank you.
Really excellent video PGT! Love you brother; really awesome to see you bringing the gold again.
Thank you sir, and everyone else out there for sharing tips, tricks and knowledge. In a lot of ways it's similar to the 3d printer circles. Great thing about having so many teks and personal tweaks (for all skill levels) is that you can "Chinese Menu" stuff. A 5 gallon bucket for cultivation, someone else's personal substrate mix, and little add on/tweaks/personal touches from 3 different people.
Funnily enough, printers are great for mixology too ;)
This was a clear and concise video and I really appreciate it.
Watching it a 2nd time already. Thank you PGT, amazing quality videos everytime.
It has been to long since you have posted a new video. Thank you again for your hardwork!
I know what I'll be doing next weekend!! Love the nice clean look. Keep it up man!!
Yes! Love your stuff man!!
Thanks for your fantastic videos. Never grown mushrooms and always wanted to learn. Thank you for explaining everything so simply that a noob can understand.
this is EXACTLY the tut I needed. I just did my own agars and previous went spore p to full fruit. you are doing gods work, and apparently we're on the same cycle.
hes the goat
I’ve had mycelium grow on top of my LC very strong strains 😎🍄❤️
damn i keep failing growing green mould
how do i get my own agar dish?
@@joshturner9766 Stick to the tek. And take every percaution to the extreme. It will work.
Man just starting out in home mycology so glad I found your channel sir 🙌🙏👏
Thank you for sharing this information with the community, and fantastic presentation no nonsense consise and clear couldn't be better, I've got some watching to do now lol lots 😀
Thanks for your hard work and sharing your experience
And thank you so much for all your videos! Almost ready to start my journey into mycology thanks to you!!!
Really informative. Well delivered. Thank you sir 🙏
From Newcastle, Australia
love your videos like this! straight to the point, thanks PGT!
Thank you @PhillyGoldenTeacher, as always you make mycology easy and fun. Cant wait to make some cultures.
I wish you the best on your journey! 😁🍄
Great video. You have helped me so much. Thank you
"I'm cracking eggs of wisdom"
stole my comment
@@rightnearthebeach is it really stealing if he thought of it a day before you??
@@justincash1573 yes, hater because my comment was before the video even premiered.
@@rightnearthebeach *yes hater,
Hahahahahah dying 😅🤣😂😹😆
THANK YOU FOR YOUR WISDOM!
Hi Teach, I just wanted to say thank you for your videos. I successfully harvested 870 wet grams of mushrooms on my first grow from your videos. :) I now have decided to take my grows out of the closet and into the entire spare bedroom. I ordered most of a mushroom lab and I am looking forward to grow #2 and then further agar plate/slant work and liquid culture production :)
@09Kromerman could you explain why people should experience psilocybin?
@@ranjanty it's amazing. Try it and find out for yourself!!
@@commiecrusher I have tried them, probably before you was born. Not that it matters. My experience wasn't amazing was more on the nauseating side. Now I just take chaga, lions mane and reishi. I'm currently trying to grow cordiceps.
I wish people would just say the amount in dry weight, I know wet is a bigger number but it's less accurate since water content varies.
@@selfactualizer2099 87dg
Thank you, PGT!! Please keep up the stellar work.🙏🏼
🙏🍄
Thanks for another very helpful tutorial, PGT!
😄
Awesome videos, thank you so much!!
Knew I saw this in one of your fine videos. Thank you, should have saved it the first time. You rock.
I set that reminder, my boy 🤙🏾🍄✨
Nice one PGT! I prefer to use Li (liquid innoculant) for anyone thats interested it is sterilised water and mycelium (no nutrients) straight into grain.
I do the step you do at 8:55 with the agar plate and go straight to grain, i find tjis method excellent.
I never thought about sucking up the mycelium directly from the agar dish until i watched this so thank you for that.
I love your videos PGT ✌️
Late reply, but how do you find that method working for you? Going from the agar cup with the LC liquid right into grains?
Man this is a great video, made it very clear and simple!! Thank you!!
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Great video!!!! Thank you brother🙏🏻
OMG it’s here!!!
Thank you very informative lesson.. I made the mistake of using spores and it held up for about 3 weeks, then I dumped it into one of my spent cake flower pots outside and it ruined that too. Thanks PGT
Thank you for this!❤️
such good content. learning so much.
I learn so much from you. Thank You. 👍
Thank you for being a part of this journey! 🙏
I'm starting my fungi journey next week and your videos have taught me the ins and outs of fungi
I'm glad the videos were helpful! Wish you the best on your journey. You got this! 😁🍄
Well this was the LC video I needed. You're spot on with the info. I'm also regretting using spores for my first LC's. Oh well. You live you learn.
Same!
Just put some lc on agar and isolate the clean mycelium takes extra time but beats contam right?
@@sheeeitmayn4384 well surprisingly. I made two different LC''s B+ and Pink Buffalo. The PB spores LC I pulled them into 10cc syringes and I had about the same amount of contaminated jars as I had just using spores. That's using an SAB.
BUT.... I just did up some of the B+ using same exact method and so far I'm almost to 100% colonized in my grain spawn and so far I have zero contamination. Only thing I did different was instead of inoculating jars in my kitchen I did it in the bedroom. And TBH I thought it would be a disaster because I keep reptiles, and I have had issues with cobweb mold before I got into growing mushies. But everything is going good so far. I did do a serious deep clean though because the web mold is super annoying just in snake cages. Especially in mine because they basically are perfect environments for growing mold. I don't use glass tanks. They're a humidity nightmare if you want it be consistent.
Also I'm still working on probably going to end up building my own flow hood. Still doing the proper research on how a proper one is built. I almost went full on with that myco willy dudes build and learned that he doesn't mention a pre filter and I'm told this could mean the difference in getting months vs years from a HEPA filter. And right now we all need to save as much money as we possibly can.
@@diy_mushroomguy so you had some luck using a spore syringe straight to liquid culture? Im at the moment going to agar and brown rice flower jars with my spore syringes but was wondering if i could shoot some into LC jar and it work out fine??
Not only the nicest voice of all myco-teachers, also just super funny...absolutly LOVE the little deer!! Got it as a tatto on my rist..
Great video! You having the agar open while you scrape is keeping it open and exposed while cutting a chunk of agar and dropping it in keeps exposure to a minimum.
This timing couldn't have been better! Watching my slow mss inoculated grain jars grow...and wanting to learn more to speed stuff up😂 got some agar supplies in the mail now...but researching LC now too...so happy to see this video today from PGT! THANKS MAN!
Very informative in-depth information.
Thank you for all the videos, I am just getting started into mycology and can’t wait to do more studies.
I wish you the best on your journey! 🙏🍄
Out here crackin' eggs of wisdom.
Thanks for simplifying this with the measurements mL/gram conversion🤘
omg PGT the marbles in the jar are freaking brilliant
Great videos 🙌
Made an LC the other day had some minor sediment so had to redo that bad boy. Keep it up 👍
Great video sensei
I just ordered some premade agar plates as my plan was to go from spore syringe to LC. I just started learning with oyster mushrooms and didn't have to worry about that step. I'll head over to your vids on that now lol thanks for the info
Good luck on your journey! You got this! 😁
Yo my g, thank you for this guide your the best
Best videos on the web on this subject
Thank you very much
It’s very helpful and explains very clearly step by step
I’ll start my own liquid culture soon
I'm glad the video was able to help! Wish you the best on your journey! 🙏
The artwork consistently tickles my fancy! Do you sell prints of the sketches?
Nice one!Thanks
Omg!!
The Safety is #1 priority guy!!!!!
I forgot about him. I’m looking him up now! Super 😎
Waiting on pgt to make videos on isolating genetics before I start trying. Your videos are way easier to understand and keep it simple
Isn’t he basically saying grow it, pick the healthiest fruit, clone them on agar, and use that genetics?
Oh yes. And teaching us what each of the terms mean when looking at mycelium types and breeding mushrooms
ahh yeahh missed ya broo mush love!
Such a great video. Went from knowing nothing of LC to having no questions.
🙏🍄
You are a legend ❤❤❤
As always. You are the GOAT
Started 2 GT and 2 B+ dec 9 and 4 more of all PE the 22nd. One gt and one b+ didn't even spawn. Other two gonna go to bulk in a day or two. This my first ever grow. Also got agar plates and a microscope with plenty of slides. Following a lot of TH-cam videos and I'm having an absolute blast. Thanks PGT.
Sounds like you got many fun things to start with! Wish you the best on your journey, you got this! 😁🙏
@@PhillyGoldenTeacher ty sir, can u do a video on genetics or genetic manipulation, I'd like to make my own strains.
@@PhillyGoldenTeacher
Total Novice trying to avoid early mistakes. Seeking suggestions for Syringe distributors. Any recommendations?
UR THE BEST BRO
Sensei! I love you videos! I recently started using your recipe with peptone and using Boomer shroomers method of inoculation (taking part of the fruit through a syringe and injecting it in) and every jar I’ve had is cloudy. While I’m sure most of the problem is my sterile technique, I was wondering if you or anyone else has anyone tried this method of inoculation with this recipe? Is there a reason you inoculate from agar only? Thank you so much!
PGT, your the goat
You are amazing ❤
You too! 😄
Crackin eggs of wisdom. Lol. Man I need a new gasket for my pressure cooker and some spores or LC from a strain I haven’t done before and I’ll get to growing again. Right now my outdoor cyanescens patches are keeping me pretty excited. One of the patches has hundreds and I think will put up a thousand mushrooms this year. It’s so damn cool
thank you so much !
Awesome!
I just started my first 2 liquid cultures yesterday. Now I'm curious to see, if i did right =)
Thank you so much
THE best channel on the Tube
hey philly would you do a video on wedge agar to lc and also I know this might not be possible but a video on cleaning up a contam agar I know you kinda did this with your agar tek video but id like to see how you handle a fully colonized agar with some green mold on the side or something around that.
Your videos are the best
🙏
Nice!!
Gosh... you're a legend mate. After first harvest... u just mist the tub? That's it!?
Keep it up!
Sensei what do you use as your work station and chamber? Sounds like there’s a fan and the filter on the back. I would love a quick video of your set up. I’ve been doing the holes cut in a tub
Cheers Philly
First class that i learned from🎉
Ty!
Philly releases new clip..
"Yaaaaay!"
Amazing
Awesome. Too easy
😁🍄
This is exactly what I was looking for
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@@PhillyGoldenTeacher man thanks so much for these videos . I’m a baller on a budget and these are great 😂😂
thanks for making these videos. So informative. I love this shit! giggle
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Thanks
After you have removed mycelium from the agar dish, do you use the same agar dish to populate more liquid culture jars until no visible mycelium are present, or is it best to discard that agar dish because it's been exposed to air? Thank you for your amazing contributions to our knowledge.
I always have used honey for mine, just inoculated some mckennaii's. Can't wait for them to be done!
me too love, the macs
And howd it go
@@chrishudson4940 always works quite well for me and the spores seem to enjoy themselves. Plus I get a visual indicator when all the suagrs are consumed as the fluid goes from a golden hue to clear
@@funkymonk4688 so total noob ?, i just got spores syringes. would it be best to make LC now, or just wait and take baby steps w just spores into grain and or all in 1 bag? And possibly wait for some "experience" before jumping that far ahead? And thanks for the info im still in planning stages
@@chrishudson4940 I im always a big advocate for baby steps. Especially since once you get your first flush you can make a spore print and make an LC from there. Take your time, do your reaserch and everything will be fine!! Best of luck! Plus golden teacher gives great advice!! Can't go wrong listening to this man
Thank u so much
Thank you I think this was the best video ive seen yet that answered all my Questions, I will name my 1st mushroom after you lol😬
Nice video, we demand a no pressure cooker Pan Cyan tek!!
Thats whats up!!
Ur the best!
I keep a notebook handy and jot down the names of each and every extremely annoying ad and then vow to NEVER buy anything from them! Seems stupid for advertisers to place ads where they will be despised.
To be fair, aren’t most ads despised, regardless of placement? I’m telling you TH-cam red is the best, I hate ads too lol
@@jessbess394 . It’s much worse here on youtube as they interrupt even meditations. It’s their strategy to torture you into signing up for their paid service so you won’t keep being constantly interrupted. It’s just ugly because it creates resentment. No one likes to be forced to buy something against their will.
I'll be in bed by 7 am but I'm stoked for the new video when I wake up🍄
Thank you for helping me along this learning journey! I did go from Spore syringe to Liquid Culture, and so far, no signs of contamination. I don't have anything inside the jar to help with the stirring so not sure if that will hamper growth or trying to extract any LC since it's not being broken up for easier getting.
Drag the needle through the glob you want to pull and then suck up what you broke off. And this is what took to the millet the fastest.
Really late to this comment but you could sterilize a stir bar or marble and then plop it in the jar carefully in a sanitized environment.
Thank you,
😁
You should do a video on harvesting and flushes!
Hi and thank you for the great video. Could you tell me how long mycelium on the average will survive on an agar plate in the refrigerator.