Thank you for making these videos! I wish you had shown the biochemical testing to prove it was S. aureus, but love to hear your critical thinking process! Please do more!! Especially with mixed cultures!!
thank you for your videos sir , i have a request for you , can you do a video where you list the normal flora and pathogene of each microbiological sample , so that i know which bacteria is considered pathogenic for each sample in microbiology , and thank you again
wound I: drainage from rose garden wound sba: superficial wound; no normal flora noted; look for hemolysis; look for beta hemolysis; need to stab plate during setup; see hemolysis in stab (beta hemolytic strep?) creamy col choc: see 1 colony type. no normal flora; golden in color (staph aureus) no cna Mac: no growth; no gnr noted enterobact, pseudomonas; or fastidious heamopyicus gs: preliminary report: no normal skin flora, many prob staph aureus; id/susp to follow.
Thank you so much for this. So glad I stumbled upon your videos. I’ll binge watch it. I will be working in the micro department soon in the US (first time). In my career, I’ve been assigned to all areas of the lab except micro so I am excited and nervous at the same time. Anyway, S. aureus is Cat + and Coag + right? It’s also beta hemolytic. Does the confirmation end there? I wonder if we can test it in mannitol salt agar? 😊 Thanks!
HI Ran, yes S. aureus is catalase and coagulase positive. Most labs probably put the organism on an instrument like a Vitek or Walkaway for definitive identification and sensitivity testing. As for mannitol salt agar, that would depend on the lab. We never used it in the lab I worked in.
@@patricktracy9947 Hi- I work in a UK micro lab! We would ID it using the MALDI and then do sensitivity testing on the Vitek, then later do further sensitivities with antibiotic discs on Muller Hinton agar if it turned out to be an MRSA. If the automation is down, we'd do a latex test for coagulation and sub the colony onto Baird Parker agar, and possibly catalase and gram staining too :D
Good day. I was wondering if you could help me. When reading a microbiology report, the report will say 1+ Pseudomonas or 4+ Pseudomonas. What does the 1+ or 4+ mean?
Hi, you caught me at a good time. :) There are different ways of enumerating things in microbiology, and in this case I am guessing they are referring to the amount of something. 1+, 2+, 3+, 4+ could also also mean Rare, Few, Moderate, Many. When we streak a plate, we generally use four quadrants with each referring to an amount of organism present.This is just a guess since I don't know the context of the situation.
@@patricktracy9947 Thank you so much for the fast response. The explanation you gave is probably the information I need. I have a microbiology report from a lab. It identifies the pathogen as "1+ Pseudomonas". The only information I have been able to glean is a 4+ Pseudomonas infection is worse than a 1+ Pseudomonas infection. If the number corresponds to the amount of organism present, that is probably what the lab means when it notes the pathogen as 1+ Pseudomonas. Thank you!
@@patricktracy9947 sorry. What I meant to say was that if you can make a video of you plating the bacteria on the agar plate Or using the streak plate method. I’m actually interested in doing The stool culture for research. But I have no idea on how to start. Could it be possible that you can tell me how you performed the stool stool streak plating method.
These videos are to introduce students to one of the most daunting tasks in clinical microbiology, plate reading. It would be too much to include all aspects.
Just took a new micro job and this is SO helpful for review after many years away from the plates. THANK YOU for this resource!
I am glad they were of use.
Thank you for making these videos! I wish you had shown the biochemical testing to prove it was S. aureus, but love to hear your critical thinking process! Please do more!! Especially with mixed cultures!!
I will see if I can make more. Thanks
thank you for your videos sir , i have a request for you , can you do a video where you list the normal flora and pathogene of each microbiological sample , so that i know which bacteria is considered pathogenic for each sample in microbiology , and thank you again
wound I: drainage from rose garden wound
sba: superficial wound; no normal flora noted; look for hemolysis; look for beta hemolysis; need to stab plate during setup; see hemolysis in stab (beta hemolytic strep?)
creamy col
choc: see 1 colony type. no normal flora;
golden in color (staph aureus)
no cna
Mac: no growth; no gnr noted enterobact, pseudomonas; or fastidious heamopyicus
gs:
preliminary report: no normal skin flora, many prob staph aureus; id/susp to follow.
Thank you so much for this. So glad I stumbled upon your videos. I’ll binge watch it. I will be working in the micro department soon in the US (first time). In my career, I’ve been assigned to all areas of the lab except micro so I am excited and nervous at the same time. Anyway, S. aureus is Cat + and Coag + right? It’s also beta hemolytic. Does the confirmation end there? I wonder if we can test it in mannitol salt agar? 😊 Thanks!
HI Ran, yes S. aureus is catalase and coagulase positive. Most labs probably put the organism on an instrument like a Vitek or Walkaway for definitive identification and sensitivity testing. As for mannitol salt agar, that would depend on the lab. We never used it in the lab I worked in.
@@patricktracy9947 Hi- I work in a UK micro lab! We would ID it using the MALDI and then do sensitivity testing on the Vitek, then later do further sensitivities with antibiotic discs on Muller Hinton agar if it turned out to be an MRSA. If the automation is down, we'd do a latex test for coagulation and sub the colony onto Baird Parker agar, and possibly catalase and gram staining too :D
Hi@@limeycinnamon thanks for the input.
Good day. I was wondering if you could help me. When reading a microbiology report, the report will say 1+ Pseudomonas or 4+ Pseudomonas. What does the 1+ or 4+ mean?
Hi, you caught me at a good time. :) There are different ways of enumerating things in microbiology, and in this case I am guessing they are referring to the amount of something. 1+, 2+, 3+, 4+ could also also mean Rare, Few, Moderate, Many. When we streak a plate, we generally use four quadrants with each referring to an amount of organism present.This is just a guess since I don't know the context of the situation.
@@patricktracy9947 Thank you so much for the fast response. The explanation you gave is probably the information I need. I have a microbiology report from a lab. It identifies the pathogen as "1+ Pseudomonas". The only information I have been able to glean is a 4+ Pseudomonas infection is worse than a 1+ Pseudomonas infection. If the number corresponds to the amount of organism present, that is probably what the lab means when it notes the pathogen as 1+ Pseudomonas. Thank you!
@@LifewithaVent You're welcome. Just out of curiosity, which continent are you on?
@@patricktracy9947 North America.
@@LifewithaVent Thanks. I try to get an idea where comments come from.
Can you Make an video of performing the culture test
What is the culture test?
@@patricktracy9947 sorry. What I meant to say was that if you can make a video of you plating the bacteria on the agar plate Or using the streak plate method. I’m actually interested in doing The stool culture for research. But I have no idea on how to start. Could it be possible that you can tell me how you performed the stool stool streak plating method.
@@mrpeanutbutter6094 this is what I teach my students.
TH-cam will not let me insert a link here. Email me at ptracy@wvc.edu
@@patricktracy9947 hey good afternoon I already have send you a email.
sir can you please make more videos about mixed cultures
it is a coagualse positive and it is a pathogenic bacteria😀
where are schädler and KV for anaerobic?
These videos are to introduce students to one of the most daunting tasks in clinical microbiology, plate reading. It would be too much to include all aspects.