Microbiology: Normal flora versus Pathogens

This video describes how to tell the difference between normal flora/bacteria and pathogenic colonial growth on cultures from various body sites.
How do you know what to culture from a specimen's 24 hour growth? Watch and find out 😉

[@yeahthatshouldwork]

Very fun to listen to you!! I enjoy the little laughs here and there! A true microbiologist loves having fun at work. I work chemisty dept and microbiology.....the atmosphere is certainly more fun in micro 😋

[@MedicalLabLadyGill]

If you love what you do, you never work a day in your life...right?! : ) Thank you for your support!

[@InternetAholic]

Great video! This really helps supplement my micro rotation

[@mushtaqurrehman3805]

Thanks for so such a informative video

[@deemashabaneh6905]

Thanks for the video

[@hichambennia8026]

Merci

[@nhidowling2763]

Thank yofor your video. Do we do Sensi for haemophilus?

[@isc6303]

It’s very informative. Thank you from Korea :)

[@MedicalLabLadyGill]

You are so welcome! Thank you for your support!

[@MrAbhishekdavid]

thanks

[@hjsjjf6845]

Thank s so much 💓

[@MedicalLabLadyGill]

Glad to help!

[@paolarosas7883]

How can you count the colonies? and which colonies can be counted? Do you count the colonies in zone 1 or zone 4? Many Thanks

[@wendylyles2204]

Thank you so much for this. Struggling with GRN in my clinical microbiology class, (I wish there were explanations like this)

[@MedicalLabLadyGill]

Thank you for the support! I hope you are doing well!

[@wendylyles2204]

@@MedicalLabLadyGill please do more whenever you can!

[@prabirsampavlog4671]

Good

[@wansangaming3923]

Dear Madam....
Kindly show how Haemophilus , Neisseria & Streptococcus pneumoniae is isolated.
Thankyou

[@hanazhafirahhanifah8175]

thank you for the video! I have a question though, in minute 15:16 I couldn't tell which is Beta hemolysis and Alpha hemolysis :(

[@MedicalLabLadyGill]

Beta hemolysis makes a window through the agar because there is complete breakdown/digestion of the RBCs in the media because of the hemolysin (enzyme that breaks down the RBCs to liberate the nutrients inside). Alpha is a partial degradation (greening of the agar) of the RBCs in the agar meaning that there will still be intact RBCs present. At minute 15:16 we are just seeing beta Hemolysis. You can see right through the agar to clearly read the sharpie writing on the back of the plate and items behind the plate (furniture, the lights on the ceiling).

[@hanazhafirahhanifah8175]

@@MedicalLabLadyGill AH yes now I understand! I think my english is getting bad now too xO thank you!

[@Asghar779]

I like your voice and Lough 😍

[@MedicalLabLadyGill]

Thank you!

[@ahmedelsakka9548]

so if someone has sore throat and tonsillitis, and on culture it revealed strept pyogen, how do i know if it is the pathogenic or the normal flora one ?? because strept pyogen is normal flora of throat

[@MedicalLabLadyGill]

Thank you for the question! S. pyogenes can be carried by asymptomatic individuals. If a patient's clinical presentation suggests a bacterial infection and the organism showing up in culture is S. pyogenes, then it would be considered Strep Throat.

[@PobrengGuapo]

i like your voice and laugh

[@MedicalLabLadyGill]

Thank you!

[@Thesmile095]

Streptococcus pneumoniae is not normal flora of mouth or throat

[@cheninitayeb6590]

❤❤❤❤❤❤

[@Hvantress]

Why can't u just look in the actual blood itself under a microscope and see these organisms in the blood. Vs trying to get it to grow for regular eye view?

[@MedicalLabLadyGill]

We do a Gram stain from the specimen directly (for many types of specimens) and a positive blood culture can be directly put through an instrument like the MALDI TOF. However, the main purpose of culturing the bacteria is to amplify and separate them so you can do tests to ID.

[@JustAgirl764]

Oof proteus mirabillis smells so bad, i feel sorry for you hahaha

[@MedicalLabLadyGill]

LOL

[@luisvalentin289]

Thanks