PCR, rt-PCR and Real time PCR

COVID19 -SARS CoV 2 detection by rt-PCR
Polymerase chain Reaction
KARRY MULLIS in 1984 introduced PCR and got the Nobel prize.
Definition- It is a cell free technique for amplification of DNA molecule
Requirement- Thermal cycler, Taq DNA Polymerase, dNTP, Primers, DNA template
Steps- Denaturation, Annealing and Extension
Applications
Detection of viral infections
Diagnosis of Tb
Diagnosis of cancers
Prenatal diagnosis of diseases
Forensic medicine
COVID19
rt-PCR
Real time PCR
Taqman probe
SYBR green

[@DrAngryFace]

*The way you teach can memorize entire Biochem even for an Alzheimer's patient* 😹🫶

[@sandhyarathore_]

😂😂

[@ankit123aa]

Mandal aaple aabhari ahe♥️ , dhanyawad sir🙏 , watching from Sambhaji nagar 🫡

[@madhuribegum5202]

Sir i Need ur autograph 😊, i feel so happy watching ur videos. Thank you❤

[@singhankul6794]

Hii ji

[@mbbs2002]

Come to government medical College Jalgaon Maharashtra,

[@niteshrathor61]

Padho padho Bakchodi nhi

[@DrPooja-dp4pd]

@@niteshrathor61 😂😂

[@74.bhaskerpodila90]

bzsker...tqs .it's my autograph

[@prachijha8264]

.I was doing rtpcr as a microbiologist since 1 month but didn't understand the process..now I get it..
Thanku sir

[@s.r0700]

Big thank u sir🌹💗
Many of us requested u for this topic and we know that u will be there for us as always...
We are also here🙏🙏🙏🙏🙏

[@navaneeth_us]

Top-notch conceptual correlation and beautifully crafted and articulated session. Thank you so much, Sir.

[@ramakantarana2656]

Excellent sir u simply represented the wet lab techniques for us ...lots of respect for this mol bio lecturer

[@madnihassan]

Thnx sir love from Pakistan 🇵🇰

[@misthisinha3157]

Thank you very much sir 🙏.You are the best teacher of biochemistry

[@rahul554645]

Sir please make a video on glucose tolerance test as are exams are approaching and since you are the best. Thankyou so much for your work. ❤️

[@kunalrawal4979]

Thank u sir for making video on this topic , I was suggesting this video for very long . Hope you will continue this good work 🙏

[@Priyanka95911]

Thank you sir for such a beautiful explanation of lab technique....

[@khushimaurya3670]

Very well explanation sir clearly understood by anyone your way of explanation is so clear and smooth ... Thank u sir 🌼

[@kuldeepchouhan699]

Thank u sir🙏🏻🙏🏻🙏🏻
SARS COV2 ka que hmari PU exam m abhi aya tha
Thanks sir ab sb clear ho gya🙌🏻👐🏻

[@dr.jamsheedjavid4455]

Nice explanation Dr.
We hope in future we will be able to watch your lectures on some other important topics like DNA sequencing-sanger and NGS, microarray technology etc.

[@namratashirwadkar6565]

Excellent explanation 👍
Thank you sir

[@zebasiddiqui6622]

Thank you so much sir... You made this topic so easy.. wonderful explanation..👍

[@venkatesanmunusamy7319]

Intensity of fluorescence increases with increase in amplification

[@oshinibandara7129]

Thank you for the great explanation.

[@nibeditasarkar6591]

Thank you so much sir. Also for extra knowledge

[@gayathrigayu6228]

Really a super explanation sir......you make me more interested towards the subject and I don't like chemistry in 12th std but now biochemistry is a major subject .....after listening to your videos apart from micro and patho .. biochemistry is my favourite one and help to get more marks and understanding the concepts easily

[@gouthamreddy2715]

Which college are you from

[@abideen.k.pkondotty6410]

thank u very much sir ,you are an excellent teacher

[@Raja_YT23]

This is really amazing video. It helps me a lot to understand the PCR.
THANK YOU SO MUCH SIR.. ❤️

[@anshuls8861]

jsr

[@urvashishinghania9313]

You are really a nice teacher sir
Thank u 🤗🤗

[@citydiagnostic5517]

Thank you very much sir
For brief review

[@umeshagrawal9417]

Sir can you please continue with the videos of important topics for University examination specifically Genetics 🙏

[@amanofcultur9903]

Thank you sir, can you make videos on electrophoresis and ELISA too?

[@prakashchander9263]

Thank you .

[@navyasavant5107]

Nice explanation sir thank you

[@mudavathshankar4064]

Thank you very much sir🙂

[@Sunshine-ct3zq]

Much respect to you sir🙏

[@drfaria2000]

Welcome back sir....🤩🤩🤩

[@fahadpasha8857]

Loads of respect sir😊

[@rajalekshmijr149]

Thanku sir for this clear presentation

[@bathularamadevi3970]

Thank u sir... .simply explained

[@theabhigautam]

Sir, if we take one DNA molecule and attach the Taqman probe to one strand, then how the probe will increase its fluorescence, as DNA will replicate and produces multiple copies.

[@dhkm_001]

Thank u sirji

[@kishmadevi865]

Thank you so much sir

[@glowupwithnature145]

nyc explanation but one thing I would like to tell that is taq polymerase don't do endonuclease activity (non proofreading)

[@shubhamaggarwal3801]

Thank you sir👍🏻☺️

[@medicaleducation8978]

Sir in reverse transcriptase PCR we are not using detectors or not ?

[@spshivani6828]

Amazing 🙏 sir 🙏🙏🙏🙏👍👍👍👍👍

[@drsachin246]

Thanks.

[@Vaguspostedat]

Thank you sir better than reading a book

[@Doctor__22]

Thank you so much sir ❤️

[@RAMBABU-wx4ee]

Thanks sir 🙏🙏

[@prajwalareddy2603]

Thank u so much Sir

[@kudikamaal2840]

Uh are few days late else this cud be helpful for my xam😅😅btw Always great explanation Sir ....keep up the good work ....☺️

[@kudikamaal2840]

@@dr.derbyallen7063 😶😶sorry sir 🤣😂

[@jollygood592]

You are only... who provided vaubale lectures more than paid lecture sir....
Thank you so much sir.. thanks alot ❤❤❤

[@uzairqureshi3605]

sir thankyou!

[@vaibhavsisodiya2109]

Thank you sir

[@sohanyadav5272]

Thank you sir 🙏🙏🙏🙏🙏

[@icshahid]

Love sir ❤️

[@abhishekmishra8215]

Sir please upload video on blotting techniques,gene therapy, bioinformatics, arterial blood gas analysis

[@s.r0700]

Sir ab dna sequencing aur dna chips,vntr,rlfp etc ke upar bhi video banana pls

[@mohitsharma-vo5gi]

Thanks a lot sir 🥰🥰

[@niloferishrath2461]

sir can you take the next topic on" meister cycle "?

[@JUSTFun-fo7sn]

Excellent

[@GaganNarangOfficial]

Sir please tell me a simple words ....rt-PCR and PCR are same or not...please reply sir ji....

[@souravmohanty1200]

Thanks sir 🙂🙏

[@Thecaveandcognition]

so then, are all pcr tests for covid 19 the same?????

[@deviprasanna5694]

Sir please explain biosynthesis of triacylglyserides

[@belexhanns6024]

Simplified PCR thanks

[@kumar7sachin]

Sir please make new vedio on molecular genetics old video sound not clear

[@MAni-wm1lc]

Can I travel with pcr test instead of rt pcr

[@bhuvneshwarivala5326]

Sir please give the imp topic in extracelluar matrix and tissu Protein please sir and also water and electrolyte balance thank you sir

[@rockandshineeditings9547]

Sir next videos lo blotting techniques topics explain cheyyandi sir

[@user-bx1xk8gl5s]

👏👏👏

[@Innervoice_Sukoon]

How can i explain real time pcr in layman language by giving real life example

[@devendrasrivastva3610]

PCR thermocycler can not be used after RT is done manually for quantitative of COVID

[@legolas-yj7mp]

From knruhs 🙋🏻‍♂️..!

[@RAMBABU-wx4ee]

Sir please cancer and HIV topic

[@RohitGurjar-ny7zn]

Nice sir

[@sk-kz4sz]

Biochemistry paper 2 tomorrow🔪🥹

[@pyn0105]

Same 😭😩

[@mokallakomuraiah3747]

U didn't tell us at which prime ,

[@ragnarok9197]

🔥🔥🔥

[@Abhishek-fe7hc]

👌👌👌

[@ambikasaxena3550]

Can you please describe more about the chemistry of pcr or mention the book or site where we can find about it

[@MedicoPhillic]

Sir if you want to earn a little more through these beautiful videos then please use yt studio in laptop or mobile chrome browser(desktop mode) and add 4-8 mid roll ads there and also thank you sir for making biochy interesting 🙏

[@medmedicose7490]

❤️👌🏻

[@sachinrao4089]

👍🏿👍🏿sir

[@alagumani6559]

Sir pls start msc biochemistry online course

[@nycboxing8359]

Keep it simple!

[@FF-zv5ho]

Other virus can be there also in swab
How it detect it as covid?

[@saptarshibhattacharya]

By reverse transcription, that is by converting the viral RNA to DNA and then applying PCR

[@nitintyagi6009]

🌹🌹🌹🌹

[@chengalvavishwa5879]

What is the difference between real time PCR and reverse transcriptase pcr

[@Rajesh_Jambhulkar]

Reverse transcriptase PCR can be real time, real time PCR is live tracking of amplification

[@chengalvavishwa5879]

@@Rajesh_Jambhulkar Thank you sir

[@Noor-ty1xt]

Pct requirements
1)template strand
2) primer
3)taqpolymeras
4)dntps
Pcr machine

[@ayoubkhan6563]

Ruta master

[@mitthunishad]

Hats off to ur way of teaching..

[@amiteshvel3791]

First view 👍🏻

[@rks.]

After 30 cycle it produces billion copies not million

[@malikhussnain8374]

you are great sir g ,,,,, love for you muah

[@jeni3486]

🙌🏼

[@dtapabcd390]

14:10 RTqPCR

[@afsakhan5852]

Sir please upload types of pcr

[@wolfgangflywheel4307]

RT-PCR is a manufacturing process NOT a diagnostic tool. RT-PCR amplifies the target so that one can do further study; it does not and cannot distinguish between something that is actively infective or not infective.

[@bushrakhan-2248]

annealing required below 5 degree/

[@zameerrahi9039]

ads ki wjs sy dislike krna pra

[@angelasusan8629]

bad

[@sajadahanger3087]

I wasted my time by watching this video