Sir, you don't know how much your videos have helped a lot of people. you really don't know. I just appreciate your effort and God bless the womb that gave birth to you. Gracias
Bro you don't even know how much you helped me, years ago, when I was studying at university, and then more recently when I applied to work in a Covid testing laboratory. Your videos were what helped me get my degree, and my job, thank you so much.
Doing a Ph.D. related to molecular bio with zero background in Bio, your videos have and are helping me a lot. Can you please make a video on how to analyze sequencing data from NGS.
Thank you for excellent explanation of RT-PCR. You described it well and minutely differentiate the similarities and dissimilarity's between the similar term's.
Is there any video lecture/demonstration available on "Nucleic Acid Sequence-based Amplification"(NASBA) over your channel because I couldn't find it? If you've not, then pls it's a request to make one lecture on it.
Sir u r explaining things very well But u r getting more faster Sir if u will teach slowly I think the things will be explained more.Although ur way of teaching is best
Sir question**there is uracil in place of thymine as a base pair in RNA but in DNA we have thymine so from where did the thymine come from two RNA that have uracil in reverse transcript ion
Sir, can you make video on Polymerase Spiral Reaction (PSR) or LAMP PCR..? As there is no video on this topic on you tube and I hope I will get on this platform only . Regards.
Sir u mentioned tat rtase won't survive at higher temperature Tan 37 rite for linearising of rna we r heating it to 70 so won't tat denature tere itself
Sir I have question regarding an experiment utilising qRT-PCR: what does it mean to normalise a particular gene (in RNA form) into a GAPDH mRNA and further to cell numbers?
why cant one just use 37 degree temperature even with multiple repeats when pcr process happens doesnt it act on ( multiple strands and sequences at the same time i dont understand the thing about higher temperatures with repeats?
would we then determine the amount of dna produced by analzying its quantity on a spectrophotometer and then from that deducing the quantitry of rna present
Hi. I want to sintetize de Oligo dt(12-18) and Random Primers (d6)n and save some money. How do I have to configure de requirement (order) for the primer syntesis lab? I know that the 5' of the oligo dt must be modified wiht a PO3, but that's all I know lol... Please, help me
hey, does the rt enzyme synthesize the complementary strand of dna (cdna to dna) the second time as well? or is an DNA polymerase added to the sample to synthesize the dna strand?
Sir, you don't know how much your videos have helped a lot of people. you really don't know. I just appreciate your effort and God bless the womb that gave birth to you. Gracias
Thank you so much for appreciating my efforts. All the best for future career
@@shomusbiologyofficial KINDLY MAKE A VIDEO ON GENOMIC SELECTION AND kASP MAKERS ....IT WOULD BE A GREAT HELP
So true i do agree
Bro you don't even know how much you helped me, years ago, when I was studying at university, and then more recently when I applied to work in a Covid testing laboratory.
Your videos were what helped me get my degree, and my job, thank you so much.
Glad to hear that. Stay tuned
same i swear to god his videos are life saver
@@reilian 100% better than what university provided to learn with
You have a video on everything! Such a boss, thank you! :D
I am thrilled to bits that you are in TH-cam sharing easy to understand biology. Thumbs up buddy!
Glad to hear that
i'm on the verge of literally giving up on bio and then your videos are there. wow! thank you so much
You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
@@shomusbiologyofficial gladly doing that!
@@shomusbiologyofficial 🙏me ❤️
Sir u can't imagine how much ur videos have helped me
Thank you so much for appreciating my efforts
Sir I was suffering with a confusion in this reverse transcription all thanks to you I got all my concepts cleared thankyou sir ❤️
You're welcome
Sir . You are literally a saviour 💕 may god bless you
Thank you so much for appreciating my efforts
@@shomusbiologyofficial u deserve it all . And that's the least we can do . Even my teacher studied from your vdos. 😘💕
Doing a Ph.D. related to molecular bio with zero background in Bio, your videos have and are helping me a lot. Can you please make a video on how to analyze sequencing data from NGS.
Glad to hear that you're getting benefit from my lectures
Came here to understand RT-PCT techniques currently being used in Covid detection ❤️🔥🙏🏼
You're welcome
No bro that is Real time PCR which is qRT-PCR used in covid19 detection
U r the legend sir b4 8 years still perfectly u explained it ❤
Thank you
Excellent teacher for me ...forever sir,thank u so much sir...god bless you and ur future
Thank you so much for appreciating my efforts
love u sir....
helps a lot.
lov from kashmir 💛
Thank you so much for appreciating my efforts
So nicely explained sir...you are the best teacher forever....
Thank you. Glad you liked my lectures
@@shomusbiologyofficial most welcome sir...
What would I do without you.... Thank you so much!
You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
thnk u again sir... cleared all the doubts regarding this topic
Another nice video from you. Salute 🥰🥰👏👏
Thank you. Glad you liked my lectures
Thankyou so much sir for beautiful explanation 😄💫
You're welcome. Glad to hear that you're getting benefit from my lectures
Thanks for guiding me through medschool!!! You are awesome!!! 😊
You're welcome. Glad to hear that you're getting benefit from my lectures
sir your way of teaching is excellent 👏
Thank you so much for appreciating my efforts
Thank you so much.... sir you are awsyme ...the way of ur explanation. ..nice ..your viedo always help me..thanks once again
I got 78% from ur viedos
That's so nice to hear. Congratulations. Please subscribe and share
Thank you for excellent explanation of RT-PCR.
You described it well and minutely differentiate the similarities and dissimilarity's between the similar term's.
Thank you so much for appreciating my efforts
May God bless you sir
Thank you
you are really brilliant teacher i’ve ever seen 😇
thanks a lot. the video is so helpful for me.
You're welcome
Shomu Bro you are a legend
sir your video is very helped a lot of people, but prepeire new more advance and improve
New videos will come soon
Beautifully Explained!!
Thank you
Thank u soo much sir... ..
U r lectures help a lot...,🤗🤗🤗🤗🤗🤗🙏🙏🙏🙏🙏
Each n every single words is matter means alot😍
You're welcome
Content is good , voice is not loud
Like an expert. Great.
VERY NICE BROTHER ,ONE MEN ARMYYYYY.ALL THE BEST
+Arashi Pampaniya thank you. Glad you liked my lectures. Stay tuned
Well explained sir..Thank you so much
Thank you
sir excellent way of explanation.i must acknowledge:)
Very good explanation as other videos of yours. I like board explanation more than the animations.
Really valuable information sir....go on...all the best !
You are amazing ❤
Thank you
Thank you so much sir. I'm very grateful to you my lifetime.
Thank you so much for appreciating my efforts
Thank you for explaining
You're welcome
Superb explanation....Thanks for giving us such a better understanding regarding RT PCR.
excellent explanation. . super clear ... thank you so much!!
Another great video. Thanks. I am about to start doing RNA purification and cDNA creation so good to get a theory primer first:)
Thank you
Excellent ... very beneficial and simple thank you very much for all your unique videos, I appreciate all of them.
just wow sir!!!
I follow you lectures...
you have done a great job brother, simple and easy explained
+kashif ahmad thank you very much
Well done ! Clearly explained !
great job
Thank you so much for appreciating my efforts
Really helpful
You're welcome
@@shomusbiologyofficial I want to ask a question to you about my study method and my concepts.
I am a biotechnology student. Can you please help me?
Thanks sir for your tremendous lecture about RT-PCR.
Thank you. Glad you liked my lectures
Kamal from Nigeria, excellent
very informative lecture...😁
Thank you
U r great sir
Thank you
sir,, can u please post a lesson on Upstream and downstream processing..
Well done!
This is well and simply explained.
explained is outstanding
Is there any video lecture/demonstration available on "Nucleic Acid Sequence-based Amplification"(NASBA) over your channel because I couldn't find it? If you've not, then pls it's a request to make one lecture on it.
Sir u r explaining things very well
But u r getting more faster
Sir if u will teach slowly I think the things will be explained more.Although ur way of teaching is best
clarity is Awesome
Thanks bro help...in genetic engineer exam
You're welcome
Can u provide video on mini preparations and purification of plasmid dna
Great video. Well explained sir.
very great explanation sir👍
Sir question**there is uracil in place of thymine as a base pair in RNA but in DNA we have thymine so from where did the thymine come from two RNA that have uracil in reverse transcript ion
Thank you very much Sir. It is really helpful👌👌
Glad you liked it
Sir, can you make video on Polymerase Spiral Reaction (PSR) or LAMP PCR..? As there is no video on this topic on you tube and I hope I will get on this platform only .
Regards.
Thanks! Great job explaining
Sir u mentioned tat rtase won't survive at higher temperature Tan 37 rite for linearising of rna we r heating it to 70 so won't tat denature tere itself
Nicely Explained!
You're welcome
I am in class 11 and we are making a model of rtpcr in sci exibition , What is the function of Cdna ?? Not understood
really valuable information sir....go on...all the best
I listen more to Ur lecture s dan our college classes...
+mohit singh thank you. Glad you liked my lectures and
Sir I have question regarding an experiment utilising qRT-PCR: what does it mean to normalise a particular gene (in RNA form) into a GAPDH mRNA and further to cell numbers?
mate you are great!!!!
Thanks for making this video! You’re a great teacher!!! 👏🏼👏🏼👏🏼
Glad to hear that you're getting benefit from my lectures
it was rly helpful. Thank you so much
Thank u so much sir, it is much beneficial nd i do watch ur most of the video, it does help me a lot..!!🤗
Can you please add the sources/cite that use?
Nice sir
You're welcome
Good sir.
Please do video on Molecular markers and QTL mapping
Sure
Grt learning..thnku sir
why cant one just use 37 degree temperature even with multiple repeats when pcr process happens doesnt it act on ( multiple strands and sequences at the same time i dont understand the thing about higher temperatures with repeats?
would we then determine the amount of dna produced by analzying its quantity on a spectrophotometer and then from that deducing the quantitry of rna present
If I use two type of primer will the cDNA be one strand or multiple fragments?
Sir what is the time required for the overall process?
Please make a video on 5' and 3' RACE
Is reverse trancriptase pcr the same as cdna library? Which then is used for pcr? Im sooo confused
Can someone explain the difference between the Random primer and oligodT because he said both of them can bind to any place and start replication.
No the oligo dT primers will bind to the poly A tail ,they are not random.....whereas the random primers bind to the random sequence of the m RNA
thank you sir
You're welcome
Thank you, this was super informative
thank you so much
You're welcome
Hi. I want to sintetize de Oligo dt(12-18) and Random Primers (d6)n and save some money. How do I have to configure de requirement (order) for the primer syntesis lab? I know that the 5' of the oligo dt must be modified wiht a PO3, but that's all I know lol... Please, help me
Sir is RT-PCR , reverse transciptase PCR and qPCR is same or different?
Why Extension occurs just after the annealing( primer/probe adding)?
Does the temparature controls the dna polymerase?
Basic dna replication mechanism
Plz make video on fermentation kinetics and how to remember that
hey,
does the rt enzyme synthesize the complementary strand of dna (cdna to dna) the second time as well? or is an DNA polymerase added to the sample to synthesize the dna strand?
After cDNA formation RNAase H is added to cleave mRNA and then dNTPs are added for making ds DNA... Reverse transcriptase is not used
Iqra Batool thank you
Is Rapid Amplification of cDNA Ends (RACE) a type of Reverse Transcriptase PCR?
Sir I am following ur almost all videos.. U r giving good explanations bt ur videos are too much long.. Please make clear cut explanation.. Thank j
Thank you. Glad you liked my lectures
Are the pcr and rt pcr the same test???
Sir mrna se primer se jo complenmtary dna vo use hoga cDNA ke liye ??? Plz ans
Yes
GOOD JOB,,,,,,THANKS
sir can you explain a little bit about detection of pathogen by pcr please ?? its a earnest request
Sir, When we synthesize cDNA from RNA is that cDNA single or double stranded?
First it will be single stranded. Then it must be used as a template to make double stranded cDNA
When Double Strand DNA is formed,than Polymerase enzyme is required?