ELISA- Enzyme Linked Immunosorbent Assay

Immunochemical reaction
sensitive, specific, easy in automation
Enzymes
Substrates
Types
Direct
Indirect
Sandwich
Competitive
Uses
Detection of Peptides, proteins, hormones, bacterial antigen, viral antigen
Detection of antibodies

[@himanshuambilkar4160]

Elisa-
Enzyme linked immuno sorbent assay.
Detection of
Protein
Peptide
Hormone
Bacterial
Viral...
Ria radiation hazard
Shorten life...
Elisa no radiation
Longer shelf life
Easy automation...
Immunochemical method b/w Ag and Ab in which one of the immuno reagents is immobilized through solid phase support pvc or polystyrene through passive adsorptions.....
Enzyme-
Horseradish peroxidase
All
B glucosidase
Glu oxidase
Ach E....
Linked
Glutaldehyde
...
Assay-
Direct Ag
Indirect Ab
Sandwich Ag
Competitive small Ag...
Direct-
Substrate TMB tetra methyl benzidine
Amino salicylic acid
OPD Ortho phenyldiamine
Azino di diammonium salt...
Indirect-
Hiv...
Sandwich -
Avidin biotin chemistry
TSH..
Competitive
T3,T4.....
Thank you sir

[@salomi.s]

Tq.. for your quick notes

[@saku5117]

Thank you so much...

[@meghanac6128]

if i started gain interest in biochemistry then that's because of your explanation sir , i reall love your explanation.

[@VinaySharma-fu1sm]

The best way of explanation of ELISA ever.
JUST BY SIMPLE RESOURCES.
Traditional chalk and duster.

[@muhammadtaimurkhan1661]

You always prepare your lectures deligently i have covered many of lectures you have explained it eloquently no confusion at all.
Islamabad, Pakistan

[@rh2fitness788]

Your class more more better than my college class.... Live sir 🤎🤎🤎

[@rajushaikh1465]

Thank you sir for your ELISA class .I am a student of dmlt in State medical faculty of west bengal

[@ABULHASAN-jw1pb]

Truly you are a great teacher I have learned a lot with your help biochemistry topic.. Thank you Sir❤️

[@debranjanpal875]

Abul da Jim's er sir ma'am der vule gale tmi😢Anybody reply

[@sagnikbanik2165]

Conc is inversely proportional to colour. Sir you have mistakenly written wrong on the board.

[@nilimashirke5222]

Sir, you are explaining absolutely finely

[@asdecor9336]

Best video.... JazakAllah ❤️👍

[@meghanac6128]

ACTUALLY WE SHOULD THANK YOU SIR ....YOUR EXPLANATIONS ARE SOOOO GOOD SIR.

[@anchaltyagi3534]

BEST EXPLANATION.
THANK YOU SO MUCH SIR.

[@kuldeepchouhan699]

So so thankfull sir🙏🏻🙏🏻🙏🏻
Sir apke sare lecture bhut h easily smz aate h thanks a lot......sir✨👌🏻

[@qwertypoiuytrewq6846]

Sir you are my only favourite biochemistry teacher
Thanku soo much 😌

[@manju-gs6tt]

Thanku so much sir
Topic itna easily samjha aagya.

[@SudeeshJ-p2x]

Sir at 25:22 u ve written Conc T3 & T4 is directly proportional to colour intensity .. but u xplain that it's inversely proportional

[@fahadpasha8857]

Love you so much sir 😊thank you for making such concept easy

[@rittickadhikary6978]

Nice sir, your lecture is getting to much helpful to me sir, Thank you....💞💞💞💞

[@HAYATUKAAFTAB]

Sir, since Ag-Ab reaction is specific, how one antigen can attach with 2 different antibodies in case of sandwich ELISA

[@laxmandadhich9158]

FOA , they are not 2 different Ab , they are from different sources and one Antigen may have more than one epitope so it can bind to many Ab

[@dwinkleshaji7834]

Very informative sir..thank u so much

[@samanthaveerakoon2898]

Dear sir, In sandwich method you are not talking about incubation and washing, is it so or like competitive method it is done?

[@shaadsghanchi7385]

Sir the relation which you have given in the last it will be inversely proportional not directly

[@RameshKumar-ot7zi]

Simple and superb sir 🙏
Thankyou so much 👍

[@Valentina-vj7uq]

Best of the best explanation, salute to you Sir 🙏

[@lukkaraghavendrarao]

Excellent explaination sir thank you

[@s.kchannel1571]

Excellent explaination

[@gaganjotkaur8043]

VERY NICELY EXPLAINED EACH ASPECT OF ELISA..... LOOKING FOR THE MORE CONTENT WITH WELL DEFINED NOTES FOR EXAMINATION PURPOSE BUT THE SIGN THAT YOU HAVE USED AT THE END OF VIDEO IS OF DIRECTLY PROPOTIONAL.

[@My25052005]

Sir your teaching effect is nice

[@priyobasu1280]

Very nice explanation sir

[@ritikakaushal2534]

Finally understood.
Thankyou sir.

[@kirankarna771]

Thanks for the video sir and wating to see 100k

[@elementalbendingmaster3536]

Sir, what is Nano particle based ELISA

[@nikhilkhandelwal3927]

What is the antigen of intrest

[@satyambhola5187]

Thank you so much sir 🙏
You are god of biochemistry 🙏🙏🙏🙏🙏

[@Rajesh_Jambhulkar]

Not GOD, tried my best

[@satyambhola5187]

@@Rajesh_Jambhulkar 🙏thanks a lot sir

[@anzerkhan5682]

Which reaction occurs in ELISA

[@prakratisingh9984]

thanku 🙏

[@omkarwankhede6642]

Thank u sirji 🙏

[@humairaghauri8197]

Thank you.

[@saranyasaa8046]

Thank you sir ❤️💯🥰

[@namratagupta645]

I don't know sandwitch ELISA...pls breif describe sir

[@JUSTFun-fo7sn]

Mind blowing

[@amnazia-ec8yu]

like your confidence

[@mayankrawat5021]

sir elisa test ka result kitne der mai aa jata hai

[@aniketkawade768]

Thank you sir

[@nidhisharma1680]

Thank you so much sir!!

[@asifekbal4867]

Nice explanation

[@mahendrafurnituremarts3904]

So helpful for examinatination sir

[@MdNaushad-qr9to]

Thnku so much sir ❤️

[@bsmmedico7530]

Great❤❤

[@yes_stretch_no_stress]

Thank you so much sir

[@shaikh.700]

Thank you Sir😊

[@GurmeetKaur-qz1pz]

Thanks sir

[@AbhishekKumar-xj7lg]

Thank you sir 😊

[@saranyasaa8046]

❤️from Kerala

[@medicalbiochemistry_]

Interesting topic

[@mansirode2449]

Thank you sir🙏

[@bharush7181]

Whether bound or unbound, presence of enzyme in the well anyway gives the colour in the substrate.

[@laxmandadhich9158]

No , bcoz there is continuous washing of extra Ab/Ag , so if bound then only is colour produced

[@Ssssdffd]

very nice sir

[@bussymed7912]

Thank you so much sir for helping us.
#bussymed

[@katkaderamesh8425]

Thx sir

[@prasanna6623]

Sir can you pls tell about exosomics

[@sanjaykumarjs455]

Super sir

[@pushparajsingh2553]

Pushparaj singh
Thanking you sid

[@misbahshaikh-p5b]

soo good

[@anahabibi6615]

Direct is used to detect Ab not Ag

[@aashnamusaarrat9345]

Sir 2nd year k sub p BHI videos upload kijye sir..

[@pediatricsclinic6180]

Tq sir

[@sanjaykumar-ew6mb]

❤❤

[@hellohellogoodbye9327]

Understand nothing. 🙄

[@user-wk2gp3tu8h]

Thank you sir

[@sudhanshuverma123]

Thankyou so much sir

[@praptidonode1524]

Thanks sir

[@jaicruise9068]

Thank you so much sir

[@ManishKumar-wj3rt]

Thank you sir

[@shalaka6502]

Thank u so much sir

[@riteshtripathy5875]

Thanks sir

[@sanketpanchal077]

Thank you sir