Great explanation. One minor very mistake is that samples 14 and 15 are not in duplicate wells. If they were, you would only be able to run 39 samples in duplicate rather than 40.
Crystal clear presentation! Thank you. Just one comment, in the last part, should the standards using antigens also (instead of any proteins), to quantify the 40 samples? In addtion, is it absorbance or fluorescence?
If you're using spectrophotometer it measures absorbance, which is what I believe they explained in this video. There are elisa methods where a flurophore is used, where fluorescence would be measured. Probably 2 years too late to answer your question though.
Hello!! Your video helped improve my understanding a lot :) I was wondering if you'd be willing to share your references for this topic (specifically the ones used for this presentation)? I'd like to read more about it. Thank you so much!
So interesting! Thank you! I am studying dopamine in humans and animals when active in Animal Assisted Therapy. What do you think about dopamine detected by ELISA kits from tear fluid before, during and after an intervention?
Thank you, it was a very helpful info. If the positive control and the control spike seems to be higher than expected. What do I need to do? The standard are in specification and R2 =1. Please advice and thank you
Thx for the video! Question: How does the antigen or BSA bind to the well? is it simply through hydrophobic interaction with the polyester plate? or is there any covalent interaction between antigen and plate?
Very detailed and clear explanation including all the buffers and the reasons for different ELISA methods. Thank you!
This was super helpful! I needed to review ELISAs for my thesis project. THANK YOU!
Finally, we have a competitor of #shomusBiology. Great work.
Best explanation. Very descriptive. And explained the most common questions that I had.
Great explanation. One minor very mistake is that samples 14 and 15 are not in duplicate wells. If they were, you would only be able to run 39 samples in duplicate rather than 40.
What a helpful media! Thanks for your explanation.(Sang Jae Lee , DKU in Korea)
Thanks again! It is the fourth video from you that I watch and for the fourth time I really enjoyed a brilliant explanation.
This is so good! thank you so much for simple comprehensive explanation and nice visuals !!
Please don’t stop making videos! You’re very helpful
Thank you for that, i fully understood these methods which was really big problem for me.
Your explanation is so clear and great, I learned a lot from all your videos. Thank you so much and please keep up the great work !!!
I have seen All your videos. Great work....
It is very helpful to understand the techniques easily.....
Thank you...😊
Praise be to LORD JESUS
Really good explanation, well understood..Thank you
Back to your videos. I missed you so much.
Great video and great explanation. Thank you
Very useful videos. You made it so simple.thank u
Great video and very clear explanations! (According to the lecture, the competitive ELISA should be rather indirect by its idea).
really useful and the explanation is perfect
Many thanks for this very clear explanation.🌹
Incredibly helpful! Thank you 🙌
So...I m ready for my presentation on ELISA...Thank you so much😊😚
very comprehensive and interesting presentation
This is so comprehensive. Thank you so much.
very nice information shared keep it up stay blessed
Thank you for the clear explanation.
thank you :))) Your channel helps me a lot when reading research papers
Very complete. Thank you.
This is Super Helpful. Thank You!
Thank you mam... great explanation
Wonderful video 💯💯💯
thank you, it's so so understanable
Amazing explanation
I learned a lot about EILSA !! ( 식품영양학과 3학년 이세영 )
Thank you for the clear explanation
Wow this is anamazing lecture! Thank you!
Best video, great explanation!
Thank you so much for this... Really explanatory
Very helpful ❤❤❤ thank you
Thank you for describing very simply
Hello ma'am
Have you stopped uploading videos?
These are genuinely really helpful and it would be great if you could continue uploading 😊
Excellent presentation 👌
영양유전체학-좋은 영상 감사합니다.
Wonderful video. Keep making videos and make them detailed explaining whatever you can in great depth. Can you please make a video on CLIA?
Wow! That's a wonderful chanal. Hope it will develop.
이해하는데 도움이 되었습니다.
This was very helpful. Thank you so much.
Thank you for doing this :D it helps a lot!!!
this was very well put together
Thank you very much.
very helpful. Thank you
Very clear information. Thank you
Thank you! You’ve made it very easy to understand for entry level!
Thank you very much for this great explanation
Great video thanks a lot.👍
영양유전체학 영상 시청 완료하였습니다 좋은 영상 감사합니다.
Crystal clear presentation! Thank you. Just one comment, in the last part, should the standards using antigens also (instead of any proteins), to quantify the 40 samples? In addtion, is it absorbance or fluorescence?
If you're using spectrophotometer it measures absorbance, which is what I believe they explained in this video. There are elisa methods where a flurophore is used, where fluorescence would be measured. Probably 2 years too late to answer your question though.
Hello!! Your video helped improve my understanding a lot :) I was wondering if you'd be willing to share your references for this topic (specifically the ones used for this presentation)? I'd like to read more about it. Thank you so much!
where i can found the next video about the Ria !!!!!!!!! i can't found it
So interesting! Thank you! I am studying dopamine in humans and animals when active in Animal Assisted Therapy. What do you think about dopamine detected by ELISA kits from tear fluid before, during and after an intervention?
so clear... thank you very much
영양유전체학 영상시청완료했습니다. 도움이 되었습니다!
Awesome explanation. Thank you
Very helpful. Thank you
You can both use flat wells as concave wells actually
영상 시청 했습니다 ! 도움이 되었습니다.
Hebat
Hebat
Thank you so much for this great video
Thank you for this lecture
it's very detailed, thank you
Very helpful
soo helpful!! great work
Supr explanation🤩
thanks 😊 it's really help alot ❤️
Thank you, it was a very helpful info. If the positive control and the control spike seems to be higher than expected. What do I need to do? The standard are in specification and R2 =1. Please advice and thank you
Thank you mam.It was informative
영상 시청 완료했습니다 좋은 영상 소개 감사드립니다!!
Thanks 👍
Great clip. Thx.
Thx for the video! Question: How does the antigen or BSA bind to the well? is it simply through hydrophobic interaction with the polyester plate? or is there any covalent interaction between antigen and plate?
YOU'RE THE BEST
좋은 영상 감사합니다.
Well explained!
How do I make my blank sample in elisa
This is an amazing explanation! Thank you so much!!
Thank you !
영상시청하였습니니다!
Whats the different between capture Ab and detection Ab in Sandwich ELISA?
(영양유전체학) 영상 시청했습니다! 감사합니다
so damn usefull in the exam's rush.. thanks so much. i love u
좋은 영상 감사합니다!
Very useful!
I want learn how to coding Immunoassay Elisa IgE food and environmental Plates? ( Plates Coding techniques)?
Perfect
Hello! Your videos are very helpful.Can to you provide videos on various molecular markers?Thank you.
영상시청했습니다 감사합니다. (영양유전체)
It's pretty good, thank you so much
thank you ,so helpful
thank you ma'am, God bless you !
Fantastic explanation!! Thank you very much!
Please if you have spare time consider the possibility of making a video regarding CLIA ( Chemiluminescence )
It was great