IDvet tutorial: How to perform an ELISA test (protocol for poultry testing)

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ความคิดเห็น • 44

  • @zeeshanraza7423
    @zeeshanraza7423 4 ปีที่แล้ว +2

    WHEN we use different strips of plate ...negativ and positiv controlls are used at each step

    • @hassanalghalebi528
      @hassanalghalebi528 3 ปีที่แล้ว

      different for same diseaes or another diseases

    • @InnovativeDiagnostics
      @InnovativeDiagnostics  2 ปีที่แล้ว

      Hello, sorry for the late reply. Please send your question to info@innovative-diagnostics.com: our technical support team will be pleased to help.

  • @GaryHField
    @GaryHField 3 ปีที่แล้ว +2

    The acronyms of the test is LAM ELISA. That’s very strange.

  • @nextagro
    @nextagro ปีที่แล้ว

    shouldn't it have done that in a sterile conditions under a laminar flow?

    • @InnovativeDiagnostics
      @InnovativeDiagnostics  ปีที่แล้ว

      In most cases, ELISA testing does not have to be performed under sterile conditions or under a laminar flow.
      In some countries, however, there is a regulatory requirement to perform Avian Influenza ELISA tests in level 2 or 3 containment laboratories.

  • @divasan9281
    @divasan9281 3 ปีที่แล้ว +3

    Which insturment are you using to wash the wells? Around the 5th minute. Thanks

    • @hassanalghalebi528
      @hassanalghalebi528 3 ปีที่แล้ว

      you need washer to do that

    • @InnovativeDiagnostics
      @InnovativeDiagnostics  2 ปีที่แล้ว +1

      Hello, sorry for the late reply. There are several possibilities to wash the wells. Please contact info@innovative-diagnostics.com to ask for more information.

  • @lakshmi65432
    @lakshmi65432 2 หลายเดือนก่อน

    Mam what is the. Name of this test u did
    HIV or
    HBSAg (or)
    VDRL (or)
    HCV please say mam
    But mam the video was same as our teacher said to us thank you mam

  • @samarwalid5038
    @samarwalid5038 3 หลายเดือนก่อน

    What's the reason of high result of control positive?

  • @dvmgeelle5520
    @dvmgeelle5520 3 ปีที่แล้ว +1

    Lets share us calculation formula of
    Wash solution and Buffer

    • @InnovativeDiagnostics
      @InnovativeDiagnostics  2 ปีที่แล้ว

      Hello, sorry for the late reply. Please feel free to contact info@innovative-diagnostics.com to learn more about the calculation formula.

  • @sozanhassanein5641
    @sozanhassanein5641 ปีที่แล้ว

    How to calculate limit of detection for compitative idvet pprv elisa

    • @InnovativeDiagnostics
      @InnovativeDiagnostics  ปีที่แล้ว

      Dear Sozan, please send your question to our technical support team, who will be pleased to assist you: helpdesk@innovative-diagnostics.com. Wishing you a great day!

  • @kirankahal4754
    @kirankahal4754 3 ปีที่แล้ว +1

    Thank u so much for this

  • @zahraafakih7020
    @zahraafakih7020 3 ปีที่แล้ว +1

    What is the internal reference material used for? Like what's the purpose..?

    • @InnovativeDiagnostics
      @InnovativeDiagnostics  2 ปีที่แล้ว

      Hello, sorry for the late reply. Please contact info@innovative-diagnostics.com: our technical support team will be pleased to answer your questions and give you more details about the reference material.

    • @NawalArsalane
      @NawalArsalane ปีที่แล้ว

      I use R18

  • @saranomrosy7821
    @saranomrosy7821 11 หลายเดือนก่อน

    Howl can I get software? I get kit but I haven't software

    • @InnovativeDiagnostics
      @InnovativeDiagnostics  11 หลายเดือนก่อน +1

      Thank you very much for your interest. To request the software, please write to: [support.software@innovative-diagnostics.com]. We will be pleased to assist you in installing and using the software.

    • @saranomrosy7821
      @saranomrosy7821 11 หลายเดือนก่อน

      @@InnovativeDiagnostics thanks alot because I have more than one kit but unfortunately I can't get software

  • @amelkanani3570
    @amelkanani3570 2 ปีที่แล้ว

    I want to ask...
    Some well has more color (deep in color) than the positive control?

    • @InnovativeDiagnostics
      @InnovativeDiagnostics  2 ปีที่แล้ว

      Hello Amel,
      With indirect ELISAs, the more a sample is positive (containing more antibodies), the stronger its color will be during the revelation step.
      A sample may contain more antibodies than the positive control. Consequently, this sample will have a stronger color than the positive control.

  • @iammrm6
    @iammrm6 2 ปีที่แล้ว +1

    Is this a direct ELISA?

  • @shivaprasad6493
    @shivaprasad6493 2 ปีที่แล้ว

    Sir hiv elisha test refar rann 0.2127 nagative positive please respond any where

    • @InnovativeDiagnostics
      @InnovativeDiagnostics  2 ปีที่แล้ว

      Hello, you may send your questions to [info@innovative-diagnostics.com]. Our technical support team will be pleased to assist you in your result interpretation.

    • @shivaprasad6493
      @shivaprasad6493 2 ปีที่แล้ว

      @@InnovativeDiagnostics contact no

    • @amandabarthes6953
      @amandabarthes6953 2 ปีที่แล้ว

      @@shivaprasad6493 You will find our contact info here: www.innovative-diagnostics.com/contact-us/

    • @sallymusallam2178
      @sallymusallam2178 ปีที่แล้ว

      I'm also in for some checkups ELISA refar rann 0.15 negative positive ! In your opinion what is the problem and what is the solution !?

  • @hassanmahe8708
    @hassanmahe8708 ปีที่แล้ว

    Thank you very much very interesting

    • @InnovativeDiagnostics
      @InnovativeDiagnostics  ปีที่แล้ว

      Thank you very much for watching us and for this nice comment, very appreciated!

  • @moussaeshra6212
    @moussaeshra6212 2 ปีที่แล้ว

    I didn't understand any
    You are talking as we know all the basics
    We dont
    How did you prepare the sample

    • @InnovativeDiagnostics
      @InnovativeDiagnostics  2 ปีที่แล้ว +1

      Thank you for your interest. This video aims to describe the main steps of a poultry indirect ELISA protocol. If you need any additional information, for example on how to prepare your samples, please feel free to contact us at [info@innovative-diagnostics.com]. We will be pleased to assist you.

  • @amrosalman5411
    @amrosalman5411 2 ปีที่แล้ว

    Thanks alot

  • @ThịĐạoMai-d1h
    @ThịĐạoMai-d1h ปีที่แล้ว

    Tôi thích sản phẩm này ❤❤❤

    • @InnovativeDiagnostics
      @InnovativeDiagnostics  ปีที่แล้ว

      Thank you so much for the nice feedback! Cảm ơn bạn rất nhiều ;)

  • @AndishaAlex
    @AndishaAlex 7 หลายเดือนก่อน

    Sorry, in Amharic

    • @InnovativeDiagnostics
      @InnovativeDiagnostics  7 หลายเดือนก่อน

      Hello, you may activate the subtitle option in the toolbar at the bottom of the video. An automated translation is available in various languages, including Amharic.

  • @IbrahimAhmed-ht7rc
    @IbrahimAhmed-ht7rc 7 หลายเดือนก่อน

    😄😄😄😄🌹🌹