ELISA test procedure | what is elisa assay?
ฝัง
- เผยแพร่เมื่อ 5 ก.พ. 2025
- Elisa test procedure - This lecture explains about the elisa test principle and the elisa test procedure. The elisa test is to identify specific antigen or antibody to detect the presence of bacterial infection. Elisa test can detect the presence of antigen or antibody in patient specimen that help us to detect bacterial infection. Stay tuned to know answers to the following questions -
What is elisa test?
Elisa test procedure?
Elisa test principle?
Elisa test classification?
How elisa test is done?
What is elisa assay?
Elisa test is also known as the enzyme linked immunosorbent assay.
Stay tuned to know the elisa test explained in this video lecture.
For more information, log on to-
www.shomusbiolo...
Get Shomu's Biology DVD set here-
www.shomusbiolo...
Download the study materials here-
shomusbiology.c...
Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in TH-cam. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology-
Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store
Shomu’s Biology assignment services - www.shomusbiology.com/assignment -help
Join Online coaching for CSIR NET exam - www.shomusbiology.com/net-coaching
We are social. Find us on different sites here-
Our Website - www.shomusbiology.com
Facebook page- / shomusbiology
Twitter - / shomusbiology
SlideShare- www.slideshare.net/shomusbiology
Google plus- plus.google.co...
LinkedIn - / suman-bhattacharjee-2a...
TH-cam- / thefunsuman
Thank you for watching the video lecture on elisa test procedure and the principle of elisa test.
Legends are watching this video one day before exam 😅 btw....this video is very informative easy to understand helped me a lot. Thanks Shomu's biology
You're welcome. Glad to hear that you're getting benefit from my lectures
Mera kal exam hain😂
@@simadassaikia8588 okay carry on 😄 I think you're from Assam 🤔
@@zakiyasohana3037 Yes I am form Assam
@@simadassaikia8588 From which institute?
My favourite channel in all biology topics,,,thank you shomu's biology
Glad to hear that you're getting benefit from my lectures. Stay tuned for more
You r the warrior, our saviour. I become familiar with a topic of which I don't even know a single word and able to write it completely in exam. Thank you my teacher. ❤❤
Glad you liked it
Maine +3 apke video dekh kar khattam ki hai ,msc first year main hoon aur mujhe ye bhi pata hai k app ke vdo hinn mujhe pass kar sakti hai ,thank you sir 😁😁😁you are excellent 👍🏻👍🏻👍🏻👍🏻 learning from odisha
Thank you so much for appreciating my efforts
My favourite channel where topic is explained perfectly in a simplified way...
Thank you so much for appreciating my efforts
Hello!
we're really thankful to you for all your brilliant videos about life science. you're just giving us the breakthrough into life science for free, we do appreciate that and will also support you to grow.
Again, your explanation is so vivid, real in the sense that even a layman person could easily comprehend unlike others who will be just reading a downloaded ppt from google. continue.. your good work and may God be with you.
Now i have a confidence that i will be pass....Thank you sir 🙏
All the best for future career
Thankyou sir. I am ug student of Biotechnology and am doing my internship in medical College and continuing this topic. You explained very well sir I have understood the whole only in one hour. Thankyou plz keep up posting.
You're welcome. Glad you liked it
Thank you sir for explaining ELISA in a practical and lucid way ❤❤❤❤❤
You're welcome
I have wached many videos but i have not got the concept, like always you make my doubts crystal clear thak you so much Sir.
Glad to hear that you're getting benefit from my lectures
Thankyou so much sir... Tomorrow is my exam and I wasn't able to understand a single thing about this topic but you made it easier. Thankyou so much again. Your new subscriber 🌼🌼
You're welcome. Glad you liked it
@@shomusbiologyofficial sir today I was able to write on it perfectly.Thankyou again sir. This video helped a lot and I recommended this to my friends too.
Same case with me
me too
Thankyou so much for your videos, you've helped me study for the MCAT (medical entrance exam in the US). I genuinely have learned so much from your videos and no matter wherever I am meant to head into the future based on my results, your videos always inspire me to keep on learning and expand my horizon and breadth of knowledge. God bless:)
Thank you so much for appreciating my efforts. I am Honored. Stay tuned for more help. All the best for future career
I can clear my masters because of these types of videos by you sir.. thank you so much
You're welcome
Thank you so much for your videos. I have just passed my biochemistry exam using your videos and i got top marks. You are the Best ❤️
You're welcome
Grandeee
Very well explained! I have seen many videos on elisa before but you explained in best way .. and finall I understood the concept of this topic. Thanx alot
You're welcome. Glad you like it when
Thank you. Very nicely explained the complicated technique in such a simple way. Best Regards
Thank you so much for appreciating my efforts
This Topic became so clear because of your lecture🙏🏻🙏🏻
Thank u so much sir ☺️
You're welcome
You are indeed the best teacher!!!!!!!
Thank you
This video is very helpful. Thank you @Shomu's biology 👏🏽
You're welcome
Sir, your way of teaching is just awesome 🙏
Thank you
You surpassed uni doctors by ur amazing explanation. Thank so much for ur efforts.
Can you please download more videos for data analysis please 🙏🏻
Thank you
THANKYOU SO MUCH you made it COMPACT and very EASY thankyou sir
You're welcome. Glad to hear that you're getting benefit from my lectures
I m an undergraduate student of Biochemistry and of course so thankful to you for making our courses so easy❤
Eager to know more about your degrees and qualifications. Please share some with us so that we get motivated more. .
Salam from Bangladesh ❤
Glad to hear that you're getting benefit from my lectures
You are absolutely great.. Best wishes ❤
Such a wonderful explanation sir
Thank you very much Sir
✨✨
Favourite teacher ever
Thank you so much for appreciating my efforts
Thank you so much Shomu Ji- you are such a blessing!
You're welcome
Thankyou so much sir, I got very clear cut differences of all types of ELISA Test, very well explaination
Thank you
Thank you sir. for making my ongoing MSC. So easy for me _💕
You're welcome. Glad to hear that you're getting benefit from my lectures
Thankyou so much... watching it 3 hours before paper/
You're welcome
This video is very informative nd easy to understand
Thank you so much for appreciating my efforts
You are educating us with a best way thankyou so much sir👌👌
Thank You Sir So Much For The Video......You Explain So Well I am a BMLT 3RD year Student and Your Videos Helped Me ALot ....Keep Sharing More Videos 🤘🏻🙏🏻🥰❤️
Thank you so much for appreciating my efforts
very very well explained sir
thank u so much sir 👌👌🙏🙏🙏
You're welcome
Excellently explained ✨
Thank you
Thank you sir... Very nice informative lecture sir. and very excellent lecture sir... Thank you 💞💞💞👍🙂
Thank you so much for appreciating my efforts
Thank you sir, it was so clear and understandable..❤❤❤❤
You're welcome
Omg is this video for me👀Tomorrow i have immunology Exam🙂🌚... Plz Bless be My sir@shomus biology
Thank you so much for appreciating my efforts
@@shomusbiologyofficial it's my pleasure sir... Really you doing great work♥️😍♥️
Very helpful 😍 amazing
Thank you
Great job done... Keep moving forward...
Thank you
much of love for you my dear brother
Thank you
Best vedio ever 💥💥💥💥
Thank you
Excellent video for elisa
Thank you
thank you very much!
You're welcome
Sir you are a fabulous teacher.. Sir Please make a video on enzyme technologies too.
Sure. Thank you
Superb explanation sir....👏
Thank you so much for appreciating my efforts
What an explanation !!! 🔥🔥🔥
Thank you so much for appreciating my efforts
@@shomusbiologyofficial I was wandering here and there to make myself understand the actual concept, went through a lot of videos but this one cleared my concept,, keep spreading quality knowledge 🤍🤍
Thank you so much sir ☺️
You're welcome
Thanks 🙏
You're welcome
@@shomusbiologyofficial i want lecture notes of this topic
Thank you so much sir
You're welcome
Such a Lovely lecture 🤗
Thank you so much for appreciating my efforts
Thank you sir you are amazing 🤩
You're welcome
Thank you so much, Sir
Much respect from pakistan Sir.
Thank you so much for appreciating my efforts
Awesome 👏✨
You're welcome
If primary antibody has the affinity to bind to the well, then antigen vil also have the affinity to bind to the well n there is a chance of binding of secondary antibody to the antigen n gives us a colour in the end.
Primary antibody don't have with
Affinity to bind well but it is fixed in well
Elisa plate or well is coated with blocking agent that prevents further binding or unnecessary binding
Thank you sir
You're welcome
during washing the unbound substance is remove from the whells😊
Sir thank you so much
You're welcome
Amazing teaching sir! Sir can u tell that ELISA is specific to only proteins test or it can also be used to detect any microorganism? Im not associated with this just asking for gk!
Hello. Can you please do a video or notes on how to calculate the reagents of indirect ELISA. Please!
Okay I will try
Hi shomu.hi shomu ❤️
Hello sir can u pls create another video on flow cytometry ....this video is very helpful for me and for many students ...you are truly doing great job for students who have lots of doubts and with your educational video all doubts easily cleared
Thank you so much for appreciating my efforts. Coming soon
Sir kindly explain application.
Sir please make a video on ESBL and MBL
PLEASE DO VIDEO ON GMO Testing, and why it important to test GMO in food sample
Okay
Sir ,I hardly request you to make one video on Immunological assay .
Ok
Thank you sir ☺☺
You're welcome
Sir thnkx a lot of u 🙏🏻🙏🏻🌹🌹🌹🌹
Love from...odisha 👏🏻👏🏻
You're welcome
Sir... basic peptide of tissue .... in thic topic no any one video in youtube....so please made it
Thanks SB...
You're welcome
sir wanted to know if the intensity of colour formed has anything to do with intensity of the disease?
Must watch
Thank you
nice video
Can someone tell me what peroxide is it ?
Please make video on ABO DESCREPANCY IN DETAIL IN HINDI PLEASE DEAR SIR ❤❤
Okay
What about capture Antibody ?
Sir i can't find elisa or ria incase of hormone assay which is in my syllabus...one day left for my exam...please reply here that incase of hormone how can l use the word hormone in substitute for antigen antibody... Please sir give me reply... Nobody can tell me this answer... I am waiting sir🙏🏻🙏🏻🙏🏻🙏🏻🙏🏻
I am glad
Hi sir. Gd morning! Is secondary antibody which has linked with enzyme is similar to the primary antibody?
No. Secondary antibody binds to primary antibody and it has enzyme linked to it
Sir as you had told antibody- antibody don't bind to each other on time 12:56 in video. but in previous video of yours for topic Western blotting you told that antibody binds to antibody Fc region and shows color in solution.
But the Fc region is already attached to well or may be both antibodies are not similar to bind each other ... As per my understanding i think that might be the reason ab doesn't bind to ab
Sir ..,we want optical immuno assay vedio
Okay
Can U explain Geneious De novo assembly and Contig mapping ??
Why serum is preferred than blood in ELISA test
Worth watching 🙌
Thank you
Very well explained. I have one question why we skip washing step in direct Elisa because we are using enzyme linked antibody and in the next step have to add substrate , substrate because product by enzyme or if antibody is not linked to particular analyte but we have enzyme linked antibody and substrate in solution so is there any chance of colour production. I am so confused please explain
Watch the other varieties of elisa lectures
Sir how to design the primary ab against ag x ?
Your qualifications my dear
Msc in Microbiology
Can I write this on exam paper??? As it is....?
If you use wrong buffer or simple water than also kit will show accurate results
sir please explain easy methods first and then go for complex methods,
All methods are already explained
In plants ELISA is different?
In the case of sandwich ELISA, we have a secondary antibody that can interact with a particular region of primary antibody then why after second washing and adding substrate it will not show color?
Cause the substrate not individually added it is linked to the sec antibody
Why will secondary antibody bind with antigen x when ag and ab follow lock and key mechanism ? when it has already bound with primary ab it shouldn’t bind with the other ab.
well, your question is very good but my point of view the answer is that although Ag Ab bind together so secondary Ab also follow the same lock and key model it does not follow any other method to bind with Ag, lock and key model does not say that one Ag Ab phenomenon always bind, does not matter how many Ag or Ab bind. Hope u got it
Where is the enzyme to add?
Does serum contain antigens!??
Sir , you are a fabulous teacher....
Sir , please, can u make a video on the working of the China vaccine "Sinopharm".. please sir...
Thank you so much for appreciating my efforts
The Direct Elisa and Sandwich Elisa is same...Correct it
Why only detects protein ?
I requested you k mery exams hyn plz made video on types of biosensors and you highlighted my comment but don't made.... disappointed
It's not that simple. I have videos lined up.
Sir which test is best for sars cov2 antibody CMIA OR ELISA
Both are good
Sir please make a video of RIA
Sure
❤❤❤❤