The principle of Real Time PCR, Reverse Transcription, quantitative rt-PCR
ฝัง
- เผยแพร่เมื่อ 1 ส.ค. 2024
- This video is an easy and full explanation about the principle of real time PCR.
For better understanding watch the previous video about the principle of PCR:
• The principle of PCR-P... - วิทยาศาสตร์และเทคโนโลยี
Good video! just a quick comment to clarify that RT-PCR is Reverse Transcription PCR and Real Time PCR is Q-PCR, for Quantitative PCR, to avoid confusion. Thanks for your video! It was helpful
Her videos are awesome! That's the reason I've put her in my '10 Biomedical Science Channels to watch' video on my own channel! Just a pity she doesn't upload anymore :( Hope for a revival!
Good explanation
Biomed Master yeah but small blunders.....she need to improve that......and she’ll become best
Intetesting!
" RT-PCR is Reverse Transcription PCR" That's what I thought. She says "Real Time" So what's the difference?
I came here crying due to I couldn't understand my class today. I left loving RT PCR. Amazing work! Thank you so so muuuuch!
Thank you for your comment, it makes me happy o hear that :)
Yes, Manuel, very handy and great video! Will help our QFT team for sure
@@biomedicalandbiologicalsci4989 Egyptians are making good videos.
@@biomedicalandbiologicalsci4989 amazing. Your videos still having big impact.
This 5 years ago video has provided me with great knowledge and insight. Thank you very much for the clear and detailed explanation
I have to admit, pretty impressive ability to comunicate, you really made it cristal clear despite english not being your first language. Thank you very much!
i am not a molecular biologist but i can follow and understand your lecture easily, now i can interpret the COVID19 RT-PCR diagnostic result just looking at the curves and figuring out CT value quite easily, thanks and keep sharing knowledge
This is the most logical progression for someone who's looking to understand PCR. Really well structured content, big up!
great video! I already have a Master in Biologie, but never was interested so much in molecular biologie and I totaly get everything about qPCR now! Thanks!!!
your channel deserves more views! i could not have done well at university without you
Thank youu .. this really makes me happy :)
@@biomedicalandbiologicalsci4989 Hello Madam, Why have you stopped making videos? Continue please
Omg thank you soooooo much so much 😭 do you know how many times i tried to understand and looking for videos seeking god to understand this pain in the neck machine?!!! You’re awesome I finally understand it again thank you so much my savior 🥺💕
I wish you had more views. You are such a good lecturer and I am really grateful for your time in uploading these videos!
Seriously, your video is totally amazing. What you have shown here, I guarantee you, most people from the field doesn't know. Just one little insignificant correction, cDNA means "complementary DNA" and don't "circle DNA".
She said cyclic not circle
@@mehwisha.salman5517 still wrong
I’m actually surprised… I’m first year university student and following the course biotechnology and society in the Netherlands and this came by real fast that I forgot exactly what is was so I looked it up again. I cannot imagine people in the field at least a lot of them not knowing about this if they specialize in this field,
Thank you! I needed to refresh this topic, and you covered it all, good job!
all mechanisms explained clearly! no insignificant details, just the main points, thank you : )
Haven't seen such a complete and educative video!!! Thank you.
It is an amazing video! thanks a lot for this explanation. Just one correction; cDNA is for "complementary" not for "cyclic" (22:04). Great job!
Thank you very much
It is a nice video with a great explanation.
It is helpful to me
Awesome. Your presentation and explanation are clear. The best lecture out there. Keep your lectures coming.
Thank you for such a simple and clear explanation between these two assays.
Clear and simple explanation. Thank you and keep up the great videos.
Another wonderful, informative and concise video, as always. Keep going
U explain well. I didn't understand RT PCR for my course. Now I understand it. U have great teaching talent.
You are an excellent tutor, very clear message. I learnt things I could not understand from other videos.
This is absolutely incredible. Just what I needed for a presentation next week.
HAHAHA, very good. Hope you put my channel as a reference :p
Biomedical and Biological Sciences I hope too! Very good informations in you video!
@@biomedicalandbiologicalsci4989 can i have this presentation as a file?
Finally I could understand the key information thanks to this video after days of another mixed incomprehensible video. Please keep doing this. Congr. 👏👏👏
Thank you for this amazing video , really i gain great knowledge about qpcr this due to you amazing lecture , i am looking forward to more lessons
This is such a helpful video. Thank you so much for it. The only thing I noticed, was the use of "probability" instead of "possibility" at 17:38, where "probability" means "very likely", in this context. English is weird, sometimes, I know. But right. Your video was extremely helpful and the concept of rtPCR is very interesting. I'm glad it's so simple, too, as I have to be able to do it really soon.
You are magnificent.
You do not leave any point without detailed explanation.
God blessings ❤
Thank you so much. The presentation was so clear. Keep up the good work.
Thank you. Your explanation is very clear and the slides you made are awesome.
Thank you madam for your awesome explanation....first I didn't understand the rt PCR before now I'm clear in this topic ...once again thank you madam
Thank you so much, it helps me alot to understand about pcr.. You explained it well so it easier to understand 👍
Your explanation is so succinct and understandable even for a non-scientist like me!
This is such an informative video! Very useful for a graduate course I'm considering reattempting. Cheers
well explained in the simplest form ever! Kudos
Well done. The best explanation I could find!
Nice effort in giving information to the beginners and even the experts. Thanks for the video
Very good video you did an amazing job simple and doesn’t miss important details, but doesn’t go into an unnecessary amount of detail.
Outstanding job very informative and easy explanation of difficult thoughts many thanks for your knowledge sharing
Thank you so much..
I did cDNA synthesis next rt-pcr it is helpful for me
Thank you so much!!! it´s a really good explanation :)
Thank you for the nice comment :)
Thank you very much for this video ! You explian very clear and easy to follow. Hope you keep doing this
much informative and loved the way you make things much easier... here go I subscribed .. tqs keep on doing this work
This what i am looking for. Thank you for your effort and nice presentation...
Good presentation .. Just an important point: cDNA is Not cyclic DNA, It's Called Complementary DNA.
Thanks for correction
Yes👍
Good presentation
@@boinualizeh4877 good
Means this cDNA undergoes this cyclic reaction so one can say.
Your step by step explanation is excellent
Thank you
this is the first time i got what rt-pcr is.thanks a lot
This video provides an easy way to understand RT-PCR and real time PCR. Thanks! Hope you can provide more video, and many people can access this page.
You are amazing it took me years to understand ft pcr. But you did it in few minutes
I dnt know how to say thank you.... i have no words.....😥😥😥 you are amazing...and this is a wonderful video, i watched more videos of you, thank you sooo much n god bless you♥️♥️♥️
This helped it finally click for me! Much appreciated.
The way u try to make us understand is just lovely.. thanks for the video and explaination 🙏🙏❤️
Really awesome video. Super helpful in clarifying this concept for me. Thank you!
This video is very good, very absorbing, you explained this methods simply and precisely. The only thing I can say is that SYBR green instead of Cyber green. It's very interesting.
Congratulations on the video, I found it well didactic.
Thanks for the video, really helpful!
props gehen raus für des geile Video feier i extrem
Wow started doing qPCR two days ago and I don’t really know what I am doing but this video really clarified a lot. Thanks. You should make a video about analyzing qPCR data using a reference gene along with a target gene. Would really appreciate that!
Thank you for the very informative video. Easy to understand and to listen to your English accent. and very great presentation skills. Thanks a lot
It was really very beautiful video and also your accent was so nice. Thanks a lot.
Clarity in ur presentation. I enjoyed it.
thank you very much. i like your explanation. best wishes from Turkey :)
Great work :) We need some videos about Flow Cytometer :)
I have a presentation on RT-QPCR next week. Thanks so much.
no doubt its an excellent presentation about RT-PCR
Hello and congratulations on your videos. I am very pleased with the amount of detail you were able to provide in such short time.
Do you have plans to make a similar video about digital PCR?
Greetings
Very well explained, thank you for the video:)
Thank you So much, this is very helpfull
amazing video i saw in my life
i appreciate u
Great explanation... And significant points covered... Keep uploading such videos.. looking forward to it...
Thank You! Excellent job!
Excellent video. I really understand several information. Thanks
This is Apt and Well Explained. Many Thanks.
thank you for this explanation that was great yea i have some difficulty when i want to know the meaning and for what we can use the the real time pcr analysis section like RN delt rn and RQ ...... so on
Thank you very much for this video! This is a very clear explanation. One question: could the non exponential plateau phase also due to the fact that all of fluorophore molecules are separated from their quenchers?
A correction: the reason of becoming non-exponential phase means there is no more reaction components anymore ( dna pol, dntps etc. run out). The explanation of becoming non-exponential phase is not dna polimerase becomes inactive. By the way, that is a great video, thank you!
dna pol running out? can an enzyme run out that way?
my course teacher said two reasons for the non-exponential phase-
1. gradual thermal inactivation of DNA pol after each denaturation step
2. reduced efficiency of primer annealing (due to increasing competition from the template)
Thank you so much miss for a such a useful and simple explanation. 15:34 you mentioned the annealing and extension occur in 3 to 5' direction while hitting the fluorescence. I think this process is from 5 to 3" but tell us about the position of the probe in case of 5 to 3. thank you so much we really appreciate your work mam. This is the first video I have seen with such an amazing animated explanation.
You are the best!
Thank you very much
Today I understood real time PCR ( nice and simplified explanation) thank you so much
Really good video! So clear!
Thanks for the amazing presentation!
Thanks for such a nice explanation, it was really helpful. Please make more videos like this.
thank you very much for what you are doing ! i love how you take your time to explain well
i do have a request video if you please, can you take some of your time to explain the ELISpot technique (enzyme-linked immunospot) thanks
Nicely Explained, thanks!!
Great video! Thank you, I finally understand this. I have only one question: How the TaqMan hybridize with the DNA at the denaturation temperature and don't "de-hibridize"? or it hybridize at annealing temperature??
Thank you so much. it is very useful for me and very well explanation
Gracias, muy interesante y muy divertido :)
you explained it so well..thank you
maravilhoso! ajudou muito!!!
This video clarified a lot.. Thanks 🤩
You are the Bioanalysis queen!
Really a nice demonstration. Thanks.
You are so good...thank you for the useful video
You explain it well friend. Thank you so much.
Very detailed explanation and vedio. Thanks
Thank you, perfect explanation!
Really helpful for refreshing the whole thing... Thank u so much...
Thank you so much , it's helped me for my academic research about diagnostic tools used for presence of Covid 19 virus
Thank you so much! It was wonderful explanation.
Really helpful. Thank for your time
Very well explained, will be helpfull to understand the basics of RT PCR, Thank you