The principle of SDS PAGE-a full and clear explanation of the technique and how does it work

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  • เผยแพร่เมื่อ 29 พ.ค. 2024
  • This video is to understand everything about SDS-PAGE, its principle, the technique, the discontinuous gel system, and more.
    2D gel Electrophoresis video:
    • The principle of 2D Ge...
    Western Blotting video:
    • Blotting Techniques/ T...
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ความคิดเห็น • 294

  • @Kevinsungeun
    @Kevinsungeun 7 ปีที่แล้ว +215

    Why can't my professor just memorise this video and rap it during the lab. Very useful!

    • @biomedicalandbiologicalsci4989
      @biomedicalandbiologicalsci4989  7 ปีที่แล้ว +14

      Haha thank you ... maybe you can recommend it to your colleagues ;)

    • @MrPridizzle
      @MrPridizzle 6 ปีที่แล้ว +3

      throw that ish on Rap Chat yo, that would be FIRE!

    • @julietten5614
      @julietten5614 6 ปีที่แล้ว +4

      your professor probs done thousands of 'gels' while doing his first research work. I m assuming he's just not interested any more:-))

    • @tartanhandbag
      @tartanhandbag 5 ปีที่แล้ว +5

      this is why professors should do research and teachers should teach. not always the case; some professors are excellent, lively, dynamic and patient teachers also ...but most just want to get back to overseeing their projects, publishing papers and finishing grant applications before the dreaded deadlines.

    • @jimmyolum4344
      @jimmyolum4344 3 ปีที่แล้ว

      Hehe

  • @gabrieldynkin5078
    @gabrieldynkin5078 6 ปีที่แล้ว +23

    I just have to say it was the best explantion i've ever heard about anything! Thank you, it answered all of my questions....:)

  • @4MoonGlade4
    @4MoonGlade4 6 ปีที่แล้ว +5

    Hello!
    This video really saved me!
    Detailed and exhaustive.
    I was studying this technique for an exam and didn't understand it really...now it's all clear.
    Thanks! 😊

  • @medinlab141
    @medinlab141 3 ปีที่แล้ว +4

    Thank you so much for the explanation, especially for the stacking gel's part! I found it very clear.

  • @saniyapamnani8494
    @saniyapamnani8494 2 ปีที่แล้ว

    This is the best video I found on the internet about SDS PAGE. Thank you so much for all the details.

  • @brindabandas3341
    @brindabandas3341 2 ปีที่แล้ว

    This is the first technique video which I have completely understood without any practical experience. Thank you so much for making such a great video.

  • @tedwills-topic9524
    @tedwills-topic9524 2 ปีที่แล้ว +3

    This helped me a lot before even doing my practicals. Thank you

  • @ismailgbadamosi8321
    @ismailgbadamosi8321 4 ปีที่แล้ว

    This is the best video I have seen on the working principle of SDS PAGE. Thank you

  • @justcat6042
    @justcat6042 6 ปีที่แล้ว

    This is the most helpful video i have ever watched about sds page. Now i understand much more about this! Thank you so much

  • @murielmirus5641
    @murielmirus5641 4 ปีที่แล้ว +3

    thank you so much for the hard work you put into this video! it is very much appreciated :) your video has helped me a lot in the lab today!

  • @ranahaddad5446
    @ranahaddad5446 2 ปีที่แล้ว

    I had watched many videos to understand this topic, but this video is definitely the best! Thank you so much!

  • @UQMD
    @UQMD 4 ปีที่แล้ว +2

    Thanks for the fantastic video! We just watched your video in class and it definitely helped us understand electrophoresis. I especially loved the pace of your video :)

  • @maharmibhattacharya4327
    @maharmibhattacharya4327 4 ปีที่แล้ว +2

    thank you so much, the explanation was very descriptive and reasonably good.

  • @daniellegoodwin3517
    @daniellegoodwin3517 3 ปีที่แล้ว

    Thanks so much for a very clear explanation! I tried to understand the technique by reading a description online but it was so confusing, thanks for clearing it up for me!

  • @richardjoy8522
    @richardjoy8522 3 ปีที่แล้ว

    Really really really good explanation...I was searching for this type of detailed explanation in whole of TH-cam.....

  • @giorgiasartori6334
    @giorgiasartori6334 3 ปีที่แล้ว

    you are so amazing, keep going like this!! this is THE ONLY VIDEO i found explained well

  • @rogsang9764
    @rogsang9764 ปีที่แล้ว

    thanks for the explanation, the best thing is that you dedicate time to explain the principle of the gel, its the best explanation i´ve seen so far :D

  • @KJKP
    @KJKP 6 ปีที่แล้ว +32

    More detailed than other videos on SDS-PAGE.

  • @swagpunjabiada6521
    @swagpunjabiada6521 6 ปีที่แล้ว +2

    Mam please keep uploading such videos so that we can have clear concept about each technique ur way of teaching is great😄

  • @RevesAbsurdes1
    @RevesAbsurdes1 6 ปีที่แล้ว +11

    Perfect ! I spend time looking for those informtion on internet and you give them (and a lot more) very clearly, good job !

    • @biomedicalandbiologicalsci4989
      @biomedicalandbiologicalsci4989  6 ปีที่แล้ว +2

      Hello, thank you for your comment ... happy you got what you need from the video :)

    • @RevesAbsurdes1
      @RevesAbsurdes1 6 ปีที่แล้ว +1

      Biomedical and Biological Sciences yes i actually have an exam about all those technics soon and you saved my life haha !

    • @biomedicalandbiologicalsci4989
      @biomedicalandbiologicalsci4989  6 ปีที่แล้ว

      Wow, this is good ... I have a playlist on my channel containing videos about all the electrophoresis techniques, have a look at it, it will help you for your exam:
      th-cam.com/video/On_ZotdZexI/w-d-xo.html

  • @mariakln9654
    @mariakln9654 5 ปีที่แล้ว +3

    Congrats! Very helpful video, I understood everything I needed for my lab. Thank you! (the explanation is very good and well constructed)

  • @harveenkaur1713
    @harveenkaur1713 5 ปีที่แล้ว +1

    The most helpful video ever! Thank you so much!

  • @sumantahalder1027
    @sumantahalder1027 7 ปีที่แล้ว +4

    I am too pleased to watch your videos, these are very clear concept growing video. Thank you very much keep it up.

  • @julymgreenday1993
    @julymgreenday1993 6 ปีที่แล้ว +4

    I finally undertood Electrophoresis!!!! THANK YOU! Greetings from Argentina ♥

  • @elmaspower2356
    @elmaspower2356 3 ปีที่แล้ว

    Awesomely explained.. Thanks very much mam. Lots of love and respect from Bangladesh

  • @joannchu14
    @joannchu14 2 หลายเดือนก่อน

    Beautiful explanation! Love this ❤

  • @francestorreysirdevan4774
    @francestorreysirdevan4774 5 ปีที่แล้ว +1

    Superb video!! Insanely helpful! Thank you so much!!

  • @MuhammadRizwan-dt5us
    @MuhammadRizwan-dt5us 6 ปีที่แล้ว +1

    i find it very useful thankyou so much for make that kind of videos

  • @roulala8993
    @roulala8993 4 ปีที่แล้ว

    Thank you very much for the video. You're a good teacher. 😊

  • @allaboutfootball5729
    @allaboutfootball5729 6 ปีที่แล้ว

    Thank u very much u r wonderful keep making these videos I learnt a lot and many things become clear

  • @shreyasen5614
    @shreyasen5614 5 ปีที่แล้ว +1

    Love the video! Very informative. Thank you so much

  • @pavanhebbar5843
    @pavanhebbar5843 2 ปีที่แล้ว

    For SDS, I just watch this... Thank you for this indepth information mam🙏🙏

  • @emilievu4846
    @emilievu4846 5 ปีที่แล้ว +11

    Very well explained! Do you have any sources for this? I would like to refer to it in my masters.

  • @adityasinha1166
    @adityasinha1166 3 ปีที่แล้ว

    Thankyou so much for the explanation. I loved it 😊😊. Good work.

  • @sundasshareef8691
    @sundasshareef8691 6 ปีที่แล้ว

    Perfect .👍 simply wht i was actually searching for🙌

  • @sylaslau302
    @sylaslau302 5 ปีที่แล้ว

    Thank you!! It's comprehensive and concise at the same time. I wonder what would be the technical difference (composition and steps) between this and Urea-PAGE for ssDNA or RNA.

  • @ashiqahamed7740
    @ashiqahamed7740 3 ปีที่แล้ว

    Very well put together. loved this video Thank you :)

  • @bobmvideos
    @bobmvideos 3 ปีที่แล้ว +7

    OMG thanks, my wife has been doing these for like 25 years, now I know what the hell she has been talking about. Now to watch your video on westerns...

  • @yeny7194
    @yeny7194 3 ปีที่แล้ว

    Thank you this helps a lot! the article i'm reading talks about 3 different gels running gel, gradient gel and stacking gel, In this video we are shown separating gel, which one of the two (running or gradient gels) would the separating gel be equivalent to?

  • @Ash-be1nv
    @Ash-be1nv ปีที่แล้ว

    extremely helpful video for beginners

  • @Laladear-sz4hz
    @Laladear-sz4hz 5 ปีที่แล้ว

    thanku for clearing my doubts about this techniques

  • @geralthasan
    @geralthasan 2 ปีที่แล้ว

    you have my thanks madam for saving me from mid exam disaster. Thank you so much

  • @bharathreddy9337
    @bharathreddy9337 6 ปีที่แล้ว +3

    I wish I will have a teacher like you in the future. terrific and detailed explanation

  • @ZAIUAMED
    @ZAIUAMED 7 ปีที่แล้ว +11

    Very helpful keep it up.

  • @soniyahalan9051
    @soniyahalan9051 6 ปีที่แล้ว +7

    Thank you so much video is very helpful to understand better SDS page

  • @alfankoshable1
    @alfankoshable1 5 ปีที่แล้ว

    thank you very informative ! keep going from Saudi arabia Salam

  • @claudiaaurie8161
    @claudiaaurie8161 6 ปีที่แล้ว +1

    very good explanation. Thank u!

  • @uzoechisamuel
    @uzoechisamuel 2 ปีที่แล้ว

    And somebody has been messing with my brain all this while. Anytime I see you in person, I will give you a bottle of win.

  • @genomics3754
    @genomics3754 4 ปีที่แล้ว

    thank you mam....for such a well explained lecture.

  • @muhammadashraf-iy1gd
    @muhammadashraf-iy1gd 8 หลายเดือนก่อน

    Outstanding explanation ❤

  • @ruchinambiar8005
    @ruchinambiar8005 3 ปีที่แล้ว

    This video was very useful. Thank you ma'am

  • @junghyunlee1714
    @junghyunlee1714 6 ปีที่แล้ว +9

    Umm... I have one question. At the stacking gel, speed of the ions is Cl(-)>protein>glycine(n/-). In my opinion, Cl is faster than glycine these distance gonna be far each other. I wanna know details what is happened in stacking gel and these speed.

  • @abidakhadim1935
    @abidakhadim1935 6 ปีที่แล้ว +1

    Thank you mam .for giving a good visual memory of SDS.page .

  • @aydamohamed7983
    @aydamohamed7983 3 ปีที่แล้ว

    Thnx sooooo much.
    Very clear and important

  • @simiralucialamberty5971
    @simiralucialamberty5971 6 ปีที่แล้ว

    Your videos are excellent!

  • @punithagr3339
    @punithagr3339 5 ปีที่แล้ว

    Clear and to the point. Thank you

  • @poetrylover5561
    @poetrylover5561 2 ปีที่แล้ว

    Very nice explanation of a difficult concept of SDS PAGE 👍👍👍👍

  • @kaushikilohiya2137
    @kaushikilohiya2137 4 ปีที่แล้ว

    This is a really great explanation of d topic

  • @avishekdas180
    @avishekdas180 4 ปีที่แล้ว

    Nicely done yet again.

  • @dancingbiologist2688
    @dancingbiologist2688 2 ปีที่แล้ว

    This was very helpful! Thank you!

  • @leticiaalvescavalcante9593
    @leticiaalvescavalcante9593 7 ปีที่แล้ว

    OH MY GOD!!!! Thank you SO much!!! I'm a student from Brazil and I was looking for something that would make me understand the electrophoresis tecnique and I was not findind anything so simple and easy like this video! I'm so happy right now... I was almost giving up haha I was reading, and reading, and reading and not understanding at all, but now is everything so clear in my mind because of you...Omg, THANK YOUUUUU!!!!!!

    • @biomedicalandbiologicalsci4989
      @biomedicalandbiologicalsci4989  7 ปีที่แล้ว +1

      WOOW .. thank you for the nice comment ... stay tuned, you might find everything you are searching for in this channel :)

  • @vavakhannan3335
    @vavakhannan3335 4 ปีที่แล้ว

    Very well explained........thank you so much mam

  • @pannalalroat5394
    @pannalalroat5394 3 ปีที่แล้ว +1

    Really such a amazing explanation 😘🤩

  • @preciousndzimandze4348
    @preciousndzimandze4348 2 ปีที่แล้ว

    So helpful, thanks so much!

  • @priyadharshini-jx6jz
    @priyadharshini-jx6jz 5 ปีที่แล้ว

    So clear ,good job 👍

  • @orfeasorfeas9346
    @orfeasorfeas9346 4 ปีที่แล้ว

    I finally understood it! Thanks!

  • @MayankSingh-uj6hi
    @MayankSingh-uj6hi 6 ปีที่แล้ว

    Very good explanation. Thank u sooo much mam

  • @TheQaseeh
    @TheQaseeh 6 ปีที่แล้ว

    thank you!! clear and detail explanation....

  • @krishnanandh7999
    @krishnanandh7999 3 ปีที่แล้ว

    Very informative. Thank you😍

  • @colin-kun3611
    @colin-kun3611 3 ปีที่แล้ว

    Solid video! Thanks a million times!
    Subbed!

  • @biotechnologymahasarakhamu4757
    @biotechnologymahasarakhamu4757 6 ปีที่แล้ว +1

    Awesome teaching. Thank you so much.

  • @danielhernandezbanuelos5581
    @danielhernandezbanuelos5581 6 ปีที่แล้ว

    thank you, very well explained.

  • @batuhantekmen6607
    @batuhantekmen6607 3 ปีที่แล้ว +2

    Why and how proteins have been aligned in the stacking gel? Thank you.

  • @darkgamingyt1659
    @darkgamingyt1659 3 ปีที่แล้ว +1

    Superb explaination

  • @shdrone94
    @shdrone94 3 ปีที่แล้ว

    very good explained !

  • @debjitdey216
    @debjitdey216 3 ปีที่แล้ว

    Finally I understood 🤗🤗🤗 thank uuuuuuu

  • @physicstutor9127
    @physicstutor9127 4 ปีที่แล้ว

    Very helpful. Thank you!

  • @AsNatCo
    @AsNatCo 2 ปีที่แล้ว +1

    Thanks Dr. what are the advantages and disadvantages of SDS page compparesmd to the native Page

  • @StalwartsCo
    @StalwartsCo 7 ปีที่แล้ว

    indeed best video for beginners.

  • @pkjaihind3472
    @pkjaihind3472 3 ปีที่แล้ว

    Excellent Explanation 👍👏👏👏
    Keep it up 👍

  • @kashmirnature3134
    @kashmirnature3134 3 ปีที่แล้ว

    Amazing' ❤️❤️
    Thanks ma'am❤️

  • @trish88881
    @trish88881 3 ปีที่แล้ว

    This is so useful. Life Saver!

  • @kanayoikeh363
    @kanayoikeh363 5 ปีที่แล้ว

    Thank you for the really great explanation. I still don't quite understand why the stacking gel is needed in the first place. Can someone please help?

  • @sadeghsadeghzadeh8170
    @sadeghsadeghzadeh8170 6 ปีที่แล้ว

    It was very useful. Thanks alot

  • @user-bc1hv2cc9p
    @user-bc1hv2cc9p 4 ปีที่แล้ว

    thank you so much it's very helpful

  • @sunnychatterjee5487
    @sunnychatterjee5487 3 ปีที่แล้ว

    Explanation is fantastic but it will very well if you explain how stacking and separating gel is prepared...

  • @ruhiyyihdyrdahl-young3846
    @ruhiyyihdyrdahl-young3846 7 ปีที่แล้ว +2

    Thanks what a great explanation!

  • @shivaprasad5446
    @shivaprasad5446 3 ปีที่แล้ว

    well explained, thanks for the video :)

  • @ikz8439
    @ikz8439 2 ปีที่แล้ว

    you're helpful thanks!

  • @realmetv2970
    @realmetv2970 ปีที่แล้ว

    Such a perfect video

  • @ArielStar
    @ArielStar 4 ปีที่แล้ว +1

    Amazingly explained, thank you!!

  • @user-eq8mc2hi3y
    @user-eq8mc2hi3y 2 ปีที่แล้ว

    Oh my god so useful video... you save my grade

  • @firasbn1582
    @firasbn1582 2 ปีที่แล้ว

    Thank you for the Video

  • @deegallarupananda6927
    @deegallarupananda6927 ปีที่แล้ว

    Thank you very much!

  • @susmita93
    @susmita93 6 ปีที่แล้ว

    Very good explanation ...really impressed..please make biochemistry molecular biology cell biology related videos to help us..thank you

  • @amosmaina1350
    @amosmaina1350 2 ปีที่แล้ว +1

    How can we distinguish the different proteins and their polymorphisms from each other after the separation.
    If, say our sample is cattle milk and we are interested in casein and all it's various polymorphism alpha, beta, kappa etc. Are we going to use the same or different markers to identify the required proteins.

  • @elenatantardini4932
    @elenatantardini4932 4 ปีที่แล้ว

    Best WB video ever!

  • @evelinjoseph5008
    @evelinjoseph5008 4 ปีที่แล้ว

    Just amazing....y der are no more videos posted? It will b great if u post about centrifuge

  • @nabilamursyidah5416
    @nabilamursyidah5416 3 ปีที่แล้ว

    thank you for the video. can you make a video of how to read the protein electrophoresis result?

  • @sushantgupta8969
    @sushantgupta8969 6 ปีที่แล้ว

    Thank u so much mam very useful god bless