The principle of PCR-Polymerase Chain Reaction, a full and easy explanation

This video explains completely and easily PCR, the technique, the principle and the protocol.
If you want to know more about DNA synthesis, press the link below:
• A full explanation abo...

[@paolagissellepicazoflores7760]

thank u random human being on the internet for explaning this before my final genetics exam (second semester med student), i hope your charger works from all angles

[@desalegnbelaytakele6267]

how was the result of your exam then?

[@kasahunfirew3592]

🔥❤️

[@atpsmpss4201]

From Spain I want to congratulate you for the wonderful videos that you do.
Your explanations are simple and at the same time very complete.
Thank you so much for your work

[@AA-gl1dr]

i could listen to you teach for hours on end. tremendously easy to understand.

[@mpfmax0]

You have the annealing temperature limits backwards: too high means no binding and too low means unspecific binding. Also you have the reverse and forward primer backwards as well on your graphic.

[@pramitanindyasaraswati9308]

Well-explained! I get a lot of benefits by watching this 20:34-minute video, I really appreciate it, thank you for sharing and looking forward to watching other videos from your channel :)

[@RohitPant04]

*Why DNA synthesis occurs in the 5'-3' direction?*
Since DNA polymerase requires a free 3' OH group for initiation of synthesis, it can synthesize in only one direction by extending the 3' end of the preexisting nucleotide chain. Hence, DNA polymerase moves along the template strand in a 3'-5' direction, and the daughter strand is formed in a 5'-3' direction.

[@Belleville197]

The best PCR explanation video I've seen so far.
Good job girl.

[@minetopcuoglu266]

Thank you. This a clear and easy explanation. You have done a better job than my lecturers in medical school.

[@ssierrat]

Thanks a lot for your videos, they are very helpful, you have a special capacity to explain complex subjects in a simple way. Thank you.

[@estellejames9993]

Thank you for instruction on a complex scientific procedure and making it understandable for a new student.

[@alwaysontherise1]

Beautifully done! Very clear explanation

[@michaeltolkachov4749]

actually, the higher the temperature of the primer annealing the higher the specificity. This is why when doing a gradient PCR, we typically take the products from the highest annealing temperature, it is also a good way to test newly synthesized primers and save time :) The denaturation time and temperature often depends on the buffer composition and used polymerase (e.g. Kapa2G has 94 and Q5 of NEB has 98 recommended) though usually, it works anyways.
I'm mentioning just in case, cool video!

[@bryanjones7126]

Outstanding overview! Well done!

[@adnan.q.753]

This was explained really nicely.

[@hayaalmly6083]

Thank you!!! you made it really easy and simple!

[@jimbean4751]

ty for this informative and educational video Especially now it's important to know how this works.

[@karenaw3428]

Thank you so much for this video! This was so awesome and helpful!

[@biomedicalandbiologicalsci4989]

Thank you for the nice comment :)

[@irinasegovia4900]

Thank you so much for your clear, simple, and engaging explanation. From Montreal.

[@marwagouda6819]

It is a very interesting way to deliver knowledge. Much thanks

[@fatimahalnasser12]

well done !! very clear explanation.

[@zargairostamkhill3926]

that was great to learn more about pcr thanks again

[@NicoBertix]

Very well explained, thanks for this...The way you say bye at the end is so cute :-)

[@aktv2137]

Thank you very much . i watched it from Bangladesh. this video is helpful for us.

[@luciachp3519]

Thank you very much, I loved the explanation

[@animeshsamanta4503]

Very beautiful explanation... Thank you very much

[@sabbirahmed8602]

wow, your explainassion is so easy. thank you

[@nadiasaad702]

Thanks for the amazing video
Your explanation is so perfect
I will follow your videos in the future
Thx

[@mervatmorad5052]

thank you, it is simple and helpful

[@fatunbarinoladayo5699]

Thanks for the detailed method of teaching..😊😊😊

[@BlackZero880]

I just Love ur way of explanation mam....from india

[@emmieali6397]

انا عربية وفهمت عليكي لغتك جدا بسيطه وسهلة. Thank u

[@nikolinakrysteva7770]

Thank you for very interesting and nice presentation of PCR. I will used to offer to my studenrs to see and to enjoy the news in the practise biochemistry. Wish you all the best and to show new methods and success in biochemistry. d-r Krusteva

[@maisasantos2654]

Thank you so much! I have a test and this video helped me a lot!!

[@biomedicalandbiologicalsci4989]

I am glade to hear that :)

[@shailashermintania4007]

Very nice & clear presentation

[@user-nz8tk7ot7d]

Thank you very much for this explanation ❤

[@aliyas8733]

Wow thank you very much you’re lifesaver

[@surendraangom9743]

Nicely explained. Thank you.

[@shauryadumka9865]

U explained it well.. great work..

[@usoewynn4705]

Thanks . Excellent explanation. Also for RT. PCR.

[@esrakayikci934]

very well explained! thank you for sharing..

[@llxu1685]

Thank you!!! It's helpful for me!!!

[@andrewmcgee1351]

Great explanation!

[@anshisview7280]

reallly good......itz help me alot...suprb explanation

[@alcepeda6843]

Highly appreciate your video, can you please add a note in the description with a correction regarding the primer binding temperature tolerances that are mentioned at 12:25.
The details are in Annie Jay's comment below. You have a great gift for teaching as is obvious in this video. Your graphics are highly effective.

[@nuwanthachamikara5823]

Thank you. Very useful content 👌

[@pritichristian2673]

Thanks. Great information

[@2morganj]

very good information, thank you...

[@poornimasirvaiya7949]

Thank you so much mam 🙏🙏 this video cleared all my doubts 🤩 really very very helpful. 🙏🙏

[@mohammedibrahim4500]

Thank you .You are special

[@yun-ching-sun]

Thanks for the clear explanation. Let me know the details of PCR or how it works.

[@zeroframe5415]

great video! thank you

[@docshaheen6741]

Excellent lecturer

[@anniesaminaiken1665]

Hi sorry I'm really confused @ 12.25 you mentioned that if the annealing temperature is too low, the primer will not bind and if the temperature is too high the primer will bind non-specifically. But I was taught the opposite and no matter how much I research into it, I find that I'm correct. if the temperature is too high, there will be no hydrogen bond formation and the primer will remain dissociate, and if the temperature is too low the primer will bond non-specifically.

[@priyankamanuja1617]

U r correct ..may be it's by mistakenly said

[@mralamtech1724]

Hello Annie

[@zifantang3792]

Thanks for correction

[@AndrewCharnley]

Looks like you were probably correct. See the commentary from:
mpfmax0
7 months ago
"...You have the annealing temperature limits backwards: too high means no binding and too low means unspecific binding. Also you have the reverse and forward primer backwards as well on your graphic..."

[@123thomas30]

you are correct, high temperature denatures the hydrogen bonds thus breaking the bond.

[@vinodvenugopal5143]

thank you, explained well

[@vincentmusilikani6717]

Thank you very much🙏🏾

[@jawharahahmed5031]

thank you so much really you made it easy

[@FlashMusic2U]

Great Video

According the Kary Mullis (inventor of the PCR technique), viral and bacterial infections aren't valid applications for PRC. Why then is this application listed here?

[@a.larson2125]

#Biomedicalandbiololicalscience , #Olivercarvajalgômez has an extremely legitimate question. Would you be so kind as to oblige with an answer?
Kary Mullis, the inventor of the PCR test had specifically stated it is an *AMPLIFICATION* test only. No different than turning the volume up on a stereo so loud the *amplifier* would blow out a speaker as would the thermal heat.

[@baghiballsakh82]

I found these two patents from Kary Mullis and his team for PCR. Might help 🤷
System for automated performance of the polymerase chain reaction (US Patent US5656493A)
This method is especially useful for performing clinical tests on the DNA or RNA from a fetus or other donor where large amounts of the DNA or RNA are not readily available and more DNA or RNA must be manufactured to have a sufficient amount to perform tests. The presence of diseases which have unique DNA or RNA signatures can be detected by amplifying a nucleic acid sample from a patient and using various probe procedures to assay for the presence of the nucleic acid sequence being detected in the test.
(Patent number: 4,965,188)
Various infectious diseases can be diagnosed by the presence in clinical samples of specific DNA sequences characteristic of the causative microorganism. These include bacteria, such as Salmonella, Chlamydia, Neis seria., viruses, such as the hepatitis viruses, and parasites, such as the Plasmodium responsible for malaria.

[@deegallarupananda6927]

Thank you very much!

[@tianyusong7735]

Best!!! Thank you!!

[@bereketwake6622]

It's interesting...thank you!!!

[@BlackZero880]

Dear mam, i just love you way of explanation, i am preparing for the interview and helping me a lot. Keep posting such valuable lecturres. Thank you very much, do you have a video on RT PCR?
plz reply

[@kedirmohammed1623]

I apreciat you

[@quratulainnuser6076]

thank you very much its superb

[@biomedicalandbiologicalsci4989]

You are welcome :)

[@dr.mohamedabdulhaleem3268]

Awesome Video and Explanation, Thank You Soo Much : )

[@luisatali]

Does the taq polymerase just stop at the end of the desired dna length (so at the end of the red marked dna in the video) or does it synthesize the rest as well?

[@sarahe.s7231]

Thank you very much ❤️

[@mingmong8599]

good explanation!

[@biomedicalandbiologicalsci4989]

Thanx ... welcome to my channel :)

[@Abdulhadi-vn7ui]

thank you so much

[@alizahranmohd4095]

Asante sana.

[@DrBill-wd1lu]

so good👍👍

[@mohameda.hassany6562]

you are excellent and amazing

[@sanjeevkumarnitrourkela6637]

Hello Ma'am...very nice explaination..

[@francescabaysa1773]

THANK YOU!

[@lekshmivg8061]

Well explained

[@ranilsamaranayaka9513]

Clear explanations, thank you.

[@ezendianefojosiemkparu1951]

TX MUM, U ARE EXCELLENCE PERSONIFIED

[@terrie001]

In the lab, how much is "one copy" of DNA? Like 10 uL of DNA sample extraction, 100 uL? Or how much do people generally start out with, you know?

[@calarzt3453]

Big Thanks for you 🌷
My specialist genetics and biotechnology..
Please video about COVID_19 PCR ..and technical procedure
And which the perfect pcr device to diagnosis this infection

[@fanyang6608]

Thank you for your wonderful explanation. I still have one question: When the elongation step starts, the dNTPs begin to bond the specific DNA sequences after the primer. But how to stop them at the terminal of the specific sequences. There is no such things like primer to stop them at the termination.

[@caroed2768]

the first two newly synthesised chains won't have the right length, they'll be longer, but after that you'll start having single strands of the right length. The first time you'll have double stranded dna of the right length (on both strands) will be cycle 3, and from there the number grows exponentially. This is clearly shown in this video th-cam.com/video/iQsu3Kz9NYo/w-d-xo.html (minute 2.00)

[@fanyang6608]

@@caroed2768 Thank you! My friend, you answered the question that had been bothering me for half a year!

[@fanyang6608]

@@aburahat4653 Thank you for your answer. I have understood the process now

[@anleyteferra7846]

Thank you!

[@rezenehabte434]

WELL PRESENTED

[@terrie001]

Thanks! Question - how does the DNA Polymerase knows when to stop the elongation step (I know it starts at the primer, but how about the end)? I guess the DNA is super long, so if my target is only 1000 base pairs, and I don't need 5000 base pairs DNA strand.

[@hendrawanbakri9363]

Terrie 000 it's about the time, basically 1 minute is time that needed to copy about 1000 bp, when you reached that limit then decrease or increase the temperature so the dna polymerase wont work properly

[@TheAlicalm]

Well done :)

[@SaiKumar-wu3ge]

Exalent mam thank for this vedio

[@rukhsorasultonova]

thank you well done

[@mohamedsamir6751]

Thank you

[@missycook1567]

Brilliant

[@user-nf8ld9th9b]

Thanks 👍👍👍

[@critthepoet9160]

Very cool

[@kamranhussain5602]

nice video

[@firas4199]

well done

[@amnaijaz7384]

Great work.
But i have a question that how do we quantify viral load as in HCV through PCR?

[@CarlosSantos-so5ww]

In order to quantify any viral load, you don't use PCR. You need to use qPCR.

[@Wyvernnnn]

Does this type of amplification yield more mutations than how would naturally occur in the human body?

[@songokukak]

Amizing keep it up

[@isuriupadhya535]

Great

[@tzatzikosouvlaki]

Could you do a video about 16s RNA sequencing ? about the 2-step PCR and how the elongation of the adapters is done. Perfect job , keep on :) :)

[@noorpk]

We can discuss about adopters.

[@tzatzikosouvlaki]

@@noorpk ok i m waiting for your next one

[@ahmedheriher3242]

thanks

[@rimel6446]

Perfect

[@bahahos7426]

Thank u

[@alitalib6342]

Hi. How can I get this presentation please?