From now on you can become a member of this channel! You will have access to some cool emojis like this: . This is of course voluntary, but the financial support helps me a lot.
Thanks. But you should draw proteins in line shape, not in folding shape.because after SDS PAGE, all folding shapes of proteins change into line shape as we use Beta-mercaptoethanole.
Thank you. Yours is the only really useful channel on YT for biochemical methods I could find. As final revision for my exams these videos are perfect - all the details pop into my mind thanks to you so clearly recapitulating the basics for me.
Why do we need two antibodies? Is it not possible to adjust the first antibody into one that can be conjugated by the peroxidase and is complementary towards the protein?
In some cases, there are primary antibodies that are already conjugated with a detection label, but these are usually limited to a few specific types of labels, and the specificity and sensitivity of these conjugated antibodies can sometimes be lower compared to using a secondary antibody. The use of two different antibodies in western blot is to increase the specificity of protein detection. This two-step process ensures that the signal generated is specific to the target protein, rather than non-specific binding of the detection reagents. It is possible to change the first antibody into one that can hold the marker, but it is highly laborious to do so. The process of conjugating a detection label to an antibody requires specialized expertise and conditions, which can be difficult to replicate for a large number of antibodies.
This is not true! Indeed the cathode for western blot is negatively charged (-) and the anode is positively charged (+) here. It is a common confusion for students, and I remember me asking the same question in one course... (the confusion comes from the fact that cations are positively charged, right?) Best Henrik
From now on you can become a member of this channel! You will have access to some cool emojis like this: .
This is of course voluntary, but the financial support helps me a lot.
Explained this better than my 4 hour lab. Thank you
Thanks. But you should draw proteins in line shape, not in folding shape.because after SDS PAGE, all folding shapes of proteins change into line shape as we use Beta-mercaptoethanole.
Thank you Henrik, brilliantly explained and simplified, keep them coming please
Thank you. Yours is the only really useful channel on YT for biochemical methods I could find. As final revision for my exams these videos are perfect - all the details pop into my mind thanks to you so clearly recapitulating the basics for me.
Excellent series of videos( enjoyed SDS-PAGE, Western Blot).
THANKS! You explaned the most important parts so well.
IT´s brilliant how you make something looks so complicated in a very simple way i love ur vidos and ur talent
The animations really help a lot to understand the concept! Thank you!!
Thank you for making me understand Western blot steps concise and clear.
Schön visuell und super verständlich erklärt! Danke dir
You have explained it in an amazing way
Explained than my professor. This is legit.
Thank you so much! I felt very lost in my lab prep but this makes so much sense now :)
this the best course in my life Thank you habibi!
Henrik, ich küsse dein Herz. Du rettest mich bei meinem Biochemie-Praktikum! Danke!
Medizin?
@@ornulusoundeffects6423Ja 😁
Such an excellent explanation
great explanation! thank you so much, now i understand western blotting!
Bro if i could give you a hug🙂
You saved me for my exams 💜
Wow.... Hats off to your explanation🔥🔥🔥🔥
Wow! Amazingly explained, very easy to understand! Thank you so much for this.
Best science channel on youtube!
Thank you Henrik!
Excellent explanation, so glad I found this video.
Happy to hear!
You helped me understand it within 4 minutes. Thanks 👍
Very well-explained, Thanks!!!!!
short. simple. easy to understand. tysm
Excellent and amazingly concise explanation! Thank you!
This was so clear to understand! Thank you so very much!
phenomenal explanation! thank you !
Helped so much ❤❤
Many thanks
Brilliantly explained. Thank you 😊
Very good video!
Truly a great video!
Omg so helpful!! Thanks for making this animation!!!
Great explanation! thank you
amazing! i got all the info for my thesis. thank u
Thanks very helpful
Very clear, thank you
Thank you so much!
is this the principle for luminex as well?
Great clear explanation
thank you henriukkkk
Abig thanks 🎉❤
Thank you.. so much
Behind helpful. Thank you.
This was so helpful, thank you!!!
Well explained thanks!
this is so helpful thanks !
Very useful
Brilliant!
Bro you are frkn amazing god bless you
Thank you!
Thank you very much 🤩
Why do we need two antibodies? Is it not possible to adjust the first antibody into one that can be conjugated by the peroxidase and is complementary towards the protein?
In some cases, there are primary antibodies that are already conjugated with a detection label, but these are usually limited to a few specific types of labels, and the specificity and sensitivity of these conjugated antibodies can sometimes be lower compared to using a secondary antibody.
The use of two different antibodies in western blot is to increase the specificity of protein detection. This two-step process ensures that the signal generated is specific to the target protein, rather than non-specific binding of the detection reagents. It is possible to change the first antibody into one that can hold the marker, but it is highly laborious to do so. The process of conjugating a detection label to an antibody requires specialized expertise and conditions, which can be difficult to replicate for a large number of antibodies.
great video
Good work
Thank you so much
very simple and amazing, thanks alot
that was so helpful☺ thank you so much
Hatts off to you 🥹♥️
perfect. thank you.
Excelente video, gracias :)
thanks! now I finally get it
thanks ,God please you
thank you kind sir
capo, gracias!
Very useful! thank you!
can you make a video on isoelectric foccusing please
Not planned at the moment, but I put it onto the long list of ideas!
Thanks
Thanks you
Thank you for good vedeo. I can development
Amazing
I apologize but isn't the anode negatively charged?
No dude thats anions! Anions are negatively charged particles that move towards anode which is positively charged :D
Thanks a lot.
Why can't they just have an enzyme limked to the first antibody, as with the way they'd carry out direct ELISA?
it is a helpful video!!
Anyone here because of MCAT prep? oooh well, maybe just me
Hi Henrik! This technique seems strikingly similar to an enzyme-linked immunoassay test. Is it non the same?
Thanks a lot 🤗
Is six weeks definitive for this analysis hiv
ajudou muito, vlw🙃
❤️❤️❤️❤️
Shukriya
Thanksss
Love ya
There is a little mistake The Cathode is + and the Anode is -
apart from that I gave you a Like (The video is perfectly explained) Thank you very much for having posted it!
This is not true!
Indeed the cathode for western blot is negatively charged (-) and the anode is positively charged (+) here. It is a common confusion for students, and I remember me asking the same question in one course... (the confusion comes from the fact that cations are positively charged, right?)
Best
Henrik
@@henrikslab I apologize you are right ! Thank you Henrik!
@@MrWhite-fm4lj No need for that... it is a classical confusion!
@@henrikslab 😄😉
Thnk u
You´re welcome, Kakashi
A ver isto pra aula de bcm da nova
you just save me
LEER LOS ARTICULOS después de ver tus videos jajaja, es ley.
讚
Good meme
Die videos auf deutsch wären nett :)
Thank you so much!!