From now on you can become a member of this channel! You will have access to some cool emojis like this: . This is of course voluntary, but the financial support helps me a lot.
Thank you. Yours is the only really useful channel on YT for biochemical methods I could find. As final revision for my exams these videos are perfect - all the details pop into my mind thanks to you so clearly recapitulating the basics for me.
Thanks. But you should draw proteins in line shape, not in folding shape.because after SDS PAGE, all folding shapes of proteins change into line shape as we use Beta-mercaptoethanole.
Why do we need two antibodies? Is it not possible to adjust the first antibody into one that can be conjugated by the peroxidase and is complementary towards the protein?
In some cases, there are primary antibodies that are already conjugated with a detection label, but these are usually limited to a few specific types of labels, and the specificity and sensitivity of these conjugated antibodies can sometimes be lower compared to using a secondary antibody. The use of two different antibodies in western blot is to increase the specificity of protein detection. This two-step process ensures that the signal generated is specific to the target protein, rather than non-specific binding of the detection reagents. It is possible to change the first antibody into one that can hold the marker, but it is highly laborious to do so. The process of conjugating a detection label to an antibody requires specialized expertise and conditions, which can be difficult to replicate for a large number of antibodies.
This is not true! Indeed the cathode for western blot is negatively charged (-) and the anode is positively charged (+) here. It is a common confusion for students, and I remember me asking the same question in one course... (the confusion comes from the fact that cations are positively charged, right?) Best Henrik
From now on you can become a member of this channel! You will have access to some cool emojis like this: .
This is of course voluntary, but the financial support helps me a lot.
Explained this better than my 4 hour lab. Thank you
Thank you Henrik, brilliantly explained and simplified, keep them coming please
Thank you. Yours is the only really useful channel on YT for biochemical methods I could find. As final revision for my exams these videos are perfect - all the details pop into my mind thanks to you so clearly recapitulating the basics for me.
THANKS! You explaned the most important parts so well.
The animations really help a lot to understand the concept! Thank you!!
Thanks. But you should draw proteins in line shape, not in folding shape.because after SDS PAGE, all folding shapes of proteins change into line shape as we use Beta-mercaptoethanole.
Schön visuell und super verständlich erklärt! Danke dir
IT´s brilliant how you make something looks so complicated in a very simple way i love ur vidos and ur talent
Thank you for making me understand Western blot steps concise and clear.
Best science channel on youtube!
Thank you so much! I felt very lost in my lab prep but this makes so much sense now :)
You have explained it in an amazing way
this the best course in my life Thank you habibi!
Such an excellent explanation
Thank you Henrik!
Excellent explanation, so glad I found this video.
Happy to hear!
Wow! Amazingly explained, very easy to understand! Thank you so much for this.
great explanation! thank you so much, now i understand western blotting!
short and sweet! thank u!
Henrik, ich küsse dein Herz. Du rettest mich bei meinem Biochemie-Praktikum! Danke!
Medizin?
@@ornulusoundeffects6423Ja 😁
You helped me understand it within 4 minutes. Thanks 👍
Truly a great video!
Very well-explained, Thanks!!!!!
Explained than my professor. This is legit.
This was so clear to understand! Thank you so very much!
Wow.... Hats off to your explanation🔥🔥🔥🔥
Brilliant!
short. simple. easy to understand. tysm
Excellent and amazingly concise explanation! Thank you!
phenomenal explanation! thank you !
Helped so much ❤❤
Many thanks
Bro if i could give you a hug🙂
You saved me for my exams 💜
Great explanation! thank you
Brilliantly explained. Thank you 😊
Omg so helpful!! Thanks for making this animation!!!
Thank you so much!
amazing! i got all the info for my thesis. thank u
Thank you very much 🤩
This was so helpful, thank you!!!
Very clear, thank you
thank you henriukkkk
Why does one need two type of antibodies? Why can't you have the first (primary) one connect to the enzyme? You wash, add substrate, done?
Thank you!
Thanks very helpful
Why do we need two antibodies? Is it not possible to adjust the first antibody into one that can be conjugated by the peroxidase and is complementary towards the protein?
In some cases, there are primary antibodies that are already conjugated with a detection label, but these are usually limited to a few specific types of labels, and the specificity and sensitivity of these conjugated antibodies can sometimes be lower compared to using a secondary antibody.
The use of two different antibodies in western blot is to increase the specificity of protein detection. This two-step process ensures that the signal generated is specific to the target protein, rather than non-specific binding of the detection reagents. It is possible to change the first antibody into one that can hold the marker, but it is highly laborious to do so. The process of conjugating a detection label to an antibody requires specialized expertise and conditions, which can be difficult to replicate for a large number of antibodies.
Very good video!
Behind helpful. Thank you.
Thank you.. so much
this is so helpful thanks !
Well explained thanks!
Bro you are frkn amazing god bless you
perfect. thank you.
very simple and amazing, thanks alot
thanks! now I finally get it
Very useful
that was so helpful☺ thank you so much
Excelente video, gracias :)
Thank you so much
Thank you ❤
Abig thanks 🎉❤
great video
Why can't they just have an enzyme limked to the first antibody, as with the way they'd carry out direct ELISA?
I’ve had the same question.
Very useful! thank you!
Good work
I apologize but isn't the anode negatively charged?
No dude thats anions! Anions are negatively charged particles that move towards anode which is positively charged :D
Thanks a lot.
Thanks you
thank you kind sir
is this the principle for luminex as well?
Great clear explanation
Hatts off to you 🥹♥️
can you make a video on isoelectric foccusing please
Not planned at the moment, but I put it onto the long list of ideas!
Thanks!
thanks ,God please you
Thanks
Amazing
capo, gracias!
it is a helpful video!!
Is six weeks definitive for this analysis hiv
Thnk u
You´re welcome, Kakashi
Thank you for good vedeo. I can development
Thanks a lot 🤗
ajudou muito, vlw🙃
❤️❤️❤️❤️
Hi Henrik! This technique seems strikingly similar to an enzyme-linked immunoassay test. Is it non the same?
Thank you bruh the WSSP does not explain any of the processes well
There is a little mistake The Cathode is + and the Anode is -
apart from that I gave you a Like (The video is perfectly explained) Thank you very much for having posted it!
This is not true!
Indeed the cathode for western blot is negatively charged (-) and the anode is positively charged (+) here. It is a common confusion for students, and I remember me asking the same question in one course... (the confusion comes from the fact that cations are positively charged, right?)
Best
Henrik
@@henrikslab I apologize you are right ! Thank you Henrik!
@@MrWhite-fm4lj No need for that... it is a classical confusion!
@@henrikslab 😄😉
Love ya
A ver isto pra aula de bcm da nova
Shukriya
Thanksss
Anyone here because of MCAT prep? oooh well, maybe just me
讚
LEER LOS ARTICULOS después de ver tus videos jajaja, es ley.
Die videos auf deutsch wären nett :)
you just save me
Good meme
Thank you so much!!