LC Troubleshooting | Retention Time Shift | how to change retention time in HPLC

LC Troubleshooting | Retention Time Shift | how to change retention time in HPLC
your quires;
Retention times must be reproducible from run to run. When problems occur, late, early or variable peak retention times may be observed. There are lots of potential causes to change in retention time.
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[@pathanraufkhan7604]

Sir please make video on Gradient Valve Test (GPV) in HPLC calibration Calculation.

[@tahiraftab1702]

How to change RT in normal phase

[@voiceofkayani5419]

By changing in polarity of mobile phase, you can do it...
More polar mobile phase increase the RT. and non polar mobile phase decrease the RT.

[@saranoor1981]

Salam sir

[@voiceofkayani5419]

Walaikum salam

[@saranoor1981]

Lamp regeneration ho sakti hy

[@saranoor1981]

Peak ae he nhi to sir kia krein. Mobile phase same ho. Peak do din pehly a rhi thi ab nhi a rhi sir is ka reason lamp energy hy.

[@voiceofkayani5419]

Bhit sarai reasons Hain will tell you..
May be sample kharab ho giya hai
May be ghalat sample ho
May be sample detorate ho giya ho
May be lamp energy issue
May be vial missing ho
May be vial position wrong ho in sequence file
May be mobile phase contaminated
May be mobile wrong choose kar Liya ho
May be method file wrong ho
May be lamdamax different ho and so on

[@voiceofkayani5419]

Recommendations is that make all preparation again freshly

[@sauravtrivedi5197]

Why lamp off during column washing and how to lamp off ?

[@voiceofkayani5419]

Dear,
During washing lamp must be off this is because,To save the lamp hours .. during washing no need of lamp and detector to be ON