I was exhausted by not finding any data regarding this topic. But this video has become a sun in darkness. I am not that smart or fast to catch these things completely. For a complete understanding of this topic, I have to watch this several times. I really appreciate this sharing of knowledge. But if u read this, I have a small query in my mind (could be a silly one): As the conc. of analyte increase, the absorption(AU) increase as well. So how can there be homogeneity b/w spectrum of two points in peak.
Hi Rahul, Happy to note that this topic is meaningful to you. For appropriate evaluation of peak purity of a component, it has to be in linear dynamic scale of PDA scale. Too high a concentration will distort the peak purity results. One should keep in mind the concept of Beer and Lambert's Law. Hope this will help.
Thank you so much, Dr. Arvind Mittal sir. This video is a really good example for understanding the peak purity. The explanation was clear and excellent, Keep posting.
Good to see you sir after such a long time. Very good presentation, helped a lot in understanding how peak purity is calculated. Keep posting more videos.
Min 14:30 of the video - when the Resolution of detector increase, better Purity Test will occur- Here the Resolution is the Spectral Resolution = the distance between two consequential Data point on the spectrum and the smaller it is, more data points has shaped the peak.
jian658 Yes voice quality is not good. However, if you use earphones/headphones then there shouldn't be any problem. Thanks for drawing my attention to this issue. I shall try to improve futher.
I was exhausted by not finding any data regarding this topic. But this video has become a sun in darkness. I am not that smart or fast to catch these things completely. For a complete understanding of this topic, I have to watch this several times. I really appreciate this sharing of knowledge.
But if u read this, I have a small query in my mind (could be a silly one): As the conc. of analyte increase, the absorption(AU) increase as well. So how can there be homogeneity b/w spectrum of two points in peak.
Hi Rahul, Happy to note that this topic is meaningful to you. For appropriate evaluation of peak purity of a component, it has to be in linear dynamic scale of PDA scale. Too high a concentration will distort the peak purity results. One should keep in mind the concept of Beer and Lambert's Law. Hope this will help.
Thank you so much, Dr. Arvind Mittal sir. This video is a really good example for understanding the peak purity. The explanation was clear and excellent, Keep posting.
Good to see you sir after such a long time. Very good presentation, helped a lot in understanding how peak purity is calculated. Keep posting more videos.
Thanks.
Good explanation sir do more videos
I need to measure glucose using this detector but I dont know the best column for this?
Thanks! Nice explanation!
Nice lecture
nice explanation
Min 14:30 of the video - when the Resolution of detector increase, better Purity Test will occur- Here the Resolution is the Spectral Resolution = the distance between two consequential Data point on the spectrum and the smaller it is, more data points has shaped the peak.
thanks
How to know the interference using 3d structure. While peak purity reduced
Kindly update hplc method development for related substance a deatiled account.
In peak purity peak .8 to 1.2 is it necessary
No! Probably you are correlating it with peak symmetry.
thank you , but can not hear very clear
jian658 Yes voice quality is not good. However, if you use earphones/headphones then there shouldn't be any problem.
Thanks for drawing my attention to this issue. I shall try to improve futher.