HPLC Chromatography Demonstration

Hi Friends 👋 😃,
Join me on Telegram - t.me/baayo_official
In this video I have done a demonstration of #HPLC Machine in collaboration with 'Spinco Biotech' company. I have first talked about the basic concepts of HPLC and its principle. Then I have demonstrated the main components of an HPLC machine and the steps involved in HPLC process, so you can relate to see How it works in real? There have been many advancements with latest HPLC machines, you will also see how we can operate HPLC experiments just with our fingertips using mobile application now. Hope this video will be useful to all students and professionals who want to learn about HPLC and see it in action.
More about this HPLC Machine here 👉 -www.shimadzu.com/an/products/...
More about Spinco Biotech here 👉 - spincotech.com/
Instagram - / baayo_official
LinkedIn - / artirani
If you just want to learn the theoretical concepts of HPLC, I have done one other video in the past, you can watch "HPLC Chromatography Basics Explained" here 👉 • HPLC Chromatography Ba...
If you have any doubts, Please leave a comment in the comments section below. 😀
Please SHARE with all your friends , SUPPORT and SUBSCRIBE to this channel ! 😀🙏🏻
👉 Subscribe: / @baayo
Other Chromatography Videos:
=========================
HPLC Chromatography 👉 • HPLC Chromatography Ba...
UV Visible Spectroscopy 👉 • UV Visible Spectroscop...
Gas Chromatography: 👉 • Gas Chromatography Pri...
#hplc #chromatography #baayo

[@BaaYo]

To access notes and practice questions, please join our Telegram channel t.me/baayo_official

[@DeepakVarma-bv3dz]

Yes

[@rajchauhan5634]

Detector ke vdo bhi bnao

[@sanikahasbe]

This video is very helpful to students because we only learn theoretically like principle, mechanism but we don't know how is HPLC instrument in real which is used in industries.There is huge difference between learning theoretical and watch the practically because while we learn theoretical only we can imagine the instrument but when we watch the video we know the how is instrument,its working.Our human brain has power to grasp pictures quickly so this video is very useful to the students because of you gave excellent explanation like instrumentation, working, components of HPLC.Mam please make more videos regarding UV,IR,Mass spectroscopy.

[@BaaYo]

thank you @saikahasbe means alot. Yes sure i will come up with all the instrumental videos

[@shreyagarg634]

Thanks a lot mma

[@reshmadeshmukh253]

True

[@hollywoodmovies129]

I have a question if in cases a new column is used then peaks areas are different from previous peak areas. What kinds of troubleshooting in the HPLC system. How to calculate RSD 2%, system sustainable

[@jollyronacher]

I’ve been in the pharmaceutical industry for 2 years now, HPLC is like the cornerstone of everything we do there. Very good video.

[@vineettiwari5473]

As Working professional in industry,the things you explained in 10 mins. I learnt it in one Month.
You're are super accurate.
Just put labsolution integration detailed video. I would greatly appreciate it.❤

[@rachunagalikar7193]

I saw your videos when I was studying B.Pharma (2018), I passed the exam (Pharmaceutical analysis) by watching your videos only.
Thanks a lot.
Love from Karnataka❤

[@BaaYo]

Thank you 😊

[@1to1000funnyvideos]

outstanding explanation 👌pdhne se phle bahut hard lag rha tha but after watching yur video ye topic bda hi easy lga

[@comewithme3660]

Thank you ma'am for the amazing demonstration... It's going to be very helpful for me. I'll save this for later.. looking forward to such an amazing demonstration.

[@user-rc5qw5xr4p]

Madam thank u so much I get most Information by your video

[@kartikeyverma6651]

Nice initiative....try to make more videos like that on Gas chromatography, Mass Spectroscopy, MS-MS, GC-MS , HPLC-MS etc. It is quite helpful...I remember the day when I saw HPLC in CSIR lab (it was one day visit to CSIR lab). One scientist there not only explained the principle but also showed us the working of HPLC, GC-MS and MS-MS and to be honest, on that day I understand the actual concept.

[@BaaYo]

Thanks!! Great to hear you got an opportunity to work with scientists and got practical lab experience. I am trying to bring the similar experience to people online so it gives a better visual understanding to everyone. Will definitely plan to cover other topics as well. 👍🏼

[@abhijeetrathore8219]

Thank you ma'am this video was very helpful for me as well i have have shared this video along with my batchmates they found it useful too in terms of getting practical knowledge about such instruments. Keep sharing practical videos ma'am with some real samples and results as you did. Ma'am i am looking for intership or training in such labs to get a practical exposure and handling such instruments if you have any oppourtunity then please share with us ma'am. Thanks alot ma'am even your lecture videos are also useful during revision of technical knowledge. @@BaaYo

[@panda8139]

Thank you so much ma'am 😊 it's quite easy to understanding the concept with practical

[@saikrishnasurajbavirisetty7804]

Mam please do videos on other instrumental analysis too, these are very helpful, appreciate your work👏👏

[@insiderweb.]

Very helpful for my job interview Thanks Mam 😊

[@HamidAli-Rph]

I love your method of communication is so wonderfull.
I watch your each and every lecture which is very helpfull

[@BaaYo]

Thank you

[@shahistabegum2008]

Trust me this is one of the phenomenal vedio on hplc thank you so much Ma'am

[@BaaYo]

Thanks a ton

[@shreyanshmishra968]

Your amazing explanation 👏

[@sonaligupta-tg5zg]

Very nice. Deeply understandable.

[@shankarpawar412]

Informative ❤

[@anjaligorai6943]

Thank you, ma'am. Really it's an interesting, informative & valuable video demonstration.

[@BaaYo]

Thank you Anjali ❤

[@kosatish]

"An outstanding demonstration of the #HPLC Machine! The comprehensive breakdown of HPLC's fundamental concepts, principles, and operational steps provides with a clear understanding of its functionality. Witnessing the integration of modern technology, like the mobile application for experiment control, showcases the remarkable advancements in HPLC. This video is an invaluable asset for students and professionals alike, offering a profound learning opportunity about HPLC in action. Kudos for sharing your expertise and collaborating to make this informative content possible!"

[@javednalband6155]

Thank you so much mam for making this video ❤❤❤❤❤❤

[@agricultureaswintamil6669]

I’m HPLC RESEARCH DEVELOPMENT ANALYST VERY GOOD explanation 😊

[@BaaYo]

Thank you.. please share this video

[@jeetcollection]

This is an insightful and knowledgeable video, ma'am. I am preparing for my interview(M.Pharm) and I came across your video to know the basics to recharge my brain and this video helped me more than I expected.

[@BaaYo]

wow thank you.. best wishes for your interview 😊

[@xyzchemistry2023]

Great Explanation

[@md.abdurrahmanrahad]

Very helpfully for us.

[@bunnyvivek8183]

Very Well explained❤❤

[@chetantake1105]

Good evening mam
Nice information and nice instrument.
May u convey me the software used to operate.
And one more, the peaks which u demonstrated it may be contamination but it is well resolved either increasing oven temperature or increase in run time.

[@nihakalita8032]

Thank you ma'am for clearing the basics

[@BaaYo]

It's my pleasure

[@udayakumartalwar568]

You are great arathi ji

[@NaveedAhmed-zn7wd]

Extreme thanks I am very much excited and want to see the energetic in the same way.....
Plz madam make video on all techniques... NMR... Mass spectrometry....
With clear instrumentations.

[@BaaYo]

Sure I will make.. thank you 😊

[@Instagram12141]

Wow.....this video is helpful 😮😮😢😢

[@lo-fi_huntsmaniax2691]

U r the best!!

[@girishchandra5568]

Very helpful,, thank you so much ma'am 🙏 UHPLC shimadzu N series ka bhi demo bata dijye plz,🙏🙏

[@AdityaRaj-zk9eh]

thanks mam

[@verygoodexpandedpande1407]

Thanks mam..nicely explained 🙌🏻🙏♥️♥️

[@BaaYo]

Thank you.. please share with others

[@Mamtakumari-tf3ep]

Wooow maam 😊😊😍😍😍 thank you for sharing with us 🙏🙏❤️♥️

[@BaaYo]

Thank you Mamta ❤

[@shampamandal3336]

Plz mam continue it... It will be very much helpful

[@BaaYo]

yes i will continue making these types of videos

[@mansoorali8256]

Ma'am please upload a video on HPLC-DAD the working principle and instrumentation

[@yousufalimazumder8350]

Need more vedios about HPLC operation

[@muskaandhingra5163]

thank you

[@murthy2108]

Excellent, Nice & Very Informative

[@BaaYo]

Thank you so much

[@supreetsingh6709]

Very nice! what is system suitability if you can explain?

[@vershachauhan775]

Thank you so much ma'am for this video

[@BaaYo]

Thank you too

[@RakeshKumar-br4tl]

Very informative vid.ma'am instrumentation series is nice initiative ma'am 👌🏻

[@BaaYo]

Glad you liked it

[@anchudhary5122]

Thank u so much ma'am for this information

[@BaaYo]

Thank you

[@Shabbir2749]

I have seen only one TH-cam channel which conceptually explains different biological techniques. Please keep it up.

[@BaaYo]

Thank you, I will

[@raktimroy3797]

Much needed❤❤❤ ICP ,xrf ke upar vi aysa banaye

[@BaaYo]

Noted

[@pradumkushwaha2733]

Very nice vedio

[@mangaleshwarprasad7936]

Maam aap bahut hi ache tarah se explain karti hai

[@BaaYo]

Thank you :)

[@user-qf9uy6tf6b]

Nice

[@kajalagrawal02]

Superb ma'am, thank you soo much👏🏻😍😍

[@BaaYo]

Yeeyy thanks Kajal ❤ hope u liked it

[@kajalagrawal02]

@@BaaYo very much mam😍

[@educationpoint778]

I Have watched many videos . but this one is too much simple and informative. i need to know how to studys the results of samples with details, if you can give me lectures in individuals i can pay you also for lectures, but i need complete concept to learn the LC operation,, i am studying in China PhD student

[@avijitmaity1649]

Can you also please tell about how to take out the fraction of sample from hplc? * the fraction whice gives peak in chromatogram...!!

[@vrajpatel5561]

Plz upload HPLC and GC Calibration videos also

[@krimislifelines999]

Thank you so much 🙏🙂😃😘😀

[@BaaYo]

Thank you too for watching and liking

[@Aditya-020]

Thank you so much mam 😊

[@BaaYo]

Please share

[@shaikhnishatbanu2693]

Outstanding 😊😊

[@BaaYo]

Many many thanks

[@shekharnalawade7249]

Thank you so much mam

[@BaaYo]

Thank you too

[@pad480]

Please make more videos HPLC Demonstrations
Software handling new method create

[@BaaYo]

Sure I will

[@santoshchauhan-pk6ly]

Thank you soo much

[@BaaYo]

You're welcome!

[@rupa_handle]

Can you provide a video of bpa detection method for shimadzu HPLC machine???

[@shubhamkumar-hu7ts]

Nice ma'am, thankyou!
Please one video for how to give commands on software

[@BaaYo]

Noted.. I will make one video for this

[@prashantghongade7521]

Please demonstrate other HPLC instrument like waters, Agilent and GC instrument also

[@BaaYo]

Sure 👍🏼, planning to create more such videos.

[@kanishjudo88]

Arti mam how samples can be detect through HPLC in same time and what is PDA detector

[@ratheradil3025]

Thanks a lot mam. Please make a video on DNA damage and repair if possible.

[@BaaYo]

Okay sure

[@ratheradil3025]

@@BaaYo Thank U

[@vershachauhan775]

Ma'am please aise or videos bnaiye hptlc or baki molecular techniques pr ...

[@BaaYo]

Yes cover krege :)

[@prajwalnalage7081]

Thank u for information

[@BaaYo]

Any time

[@rushikeshbhosale7284]

Thank you Mam 🙏

[@BaaYo]

Thank you too

[@AnusShah-yl9wd]

Love from Pakistan❤

[@afreenakhtar457]

Thank u ❤

[@BaaYo]

Please share with your batchmates

[@suryasmanmohanty9000]

How to separate the other element other than the first element which removed out from the machine, which is adsorbed by the silica..?

[@lcmotivationlifecare746]

Thank you🌹🙏

[@BaaYo]

Please share with others.. thank u

[@lcmotivationlifecare746]

👍👍👍

[@DishantPatkar]

Thank you mam 😊

[@BaaYo]

Keep watching. and thank you too

[@life.moon_to_sun]

Please tell about integration in hplc

[@pmeet4654]

Bhat din baad apaka video aya mam ap regular video banaye

[@BaaYo]

Yes regular rhegi ab.. please share with ur colleagues

[@rajchauhan5634]

Detector ke vdo bhi bnao

[@RaviKant-tb4vh]

Or other instruments per bnayo,,like Gac,UV,Mass,NMR,and other

[@dikeshathakur6141]

Give demostration on reading chromatogram

[@samiiiayyub6913]

Please explain flash chromatography

[@vijaykargirwar6950]

I am working in thalassemia unit Mumbai.

[@user-mw2ez6np2f]

Mam ❤

[@jitubhatt69]

Pl.give information about ftnir

[@priyanshabhatia209]

How to determine buffer according to drug polarity?

[@balagaravi1255]

hello how could we detect the leakage in the intrument

[@surendrapardhi2719]

Very nice

[@BaaYo]

Thanks

[@OfficialMr_nits]

👌🙏

[@BaaYo]

🙏🙏

[@parashsharma77]

❤❤❤❤❤

[@BaaYo]

❤❤

[@user-ku5jz2qg3m]

Mam ek video operate karke bhi batana Or Isme U.V.detector bhi hota hai wo kha hai

[@hanmandlumaradi9795]

What about paracetomal analysis in hplc.?

[@Monkeylluffy]

👌👌👍

[@BaaYo]

❤️

[@vijaykargirwar6950]

HPLC in thalassemia. Pl guide

[@Rajenderthakur-hf9cc]

🙏👍.

[@BaaYo]

🙏

[@GoluKumar-uv2tp]

How to make bufffer solution in hplc

[@DineshLimbu-dh1js]

Is this a same hplc instrument that is used in pharmaceutical industries ?

[@BaaYo]

Yes

[@safaltakikunji4075]

Ma'am जी, हम कैसे identify karenge ki collection me kon kon sa component है

[@harshilrana1414]

I am not able to find 34660 area can anyone please help where it is located

[@blogger.powerpoint_expert]

How to interpret that chromatogram?

[@BalwantSingh-qk8yc]

mam gc,glc kai bare mai or hplc mai pdne kai lye koi bdiya pdf yaa notes mil skte hai brifly

[@dipyamanbagchi3848]

Mam how to calcute dilution studies

[@anuragbhattacharjee4514]

Although as process engineer in pharmaceutical industry, we don't need to perform HPLC but it's a very important analysis tool to determine batch purity of drugs.

[@BaaYo]

True

[@shikaparusharam2477]

Hi madam plz tell empower audit trail