2D & 3D Cell Culture Workflows

In this video we highlight workflows related to 2D and 3D cell culture.
Additionally the presentation touches on workflow aspects related to cancer research, drug discovery as well as vaccine development.
2D cell culture was broken down into a discussion on culturing, cell plating, cell treatment followed by cell imaging and analysis. Core products in this workflow includes: cell culture media (Cytiva Hyclone), serum, tissue culture flasks and plastics (TPP) pipettors and serological pipettes (TPP & Socorex), CO2 incubators, autoclaves, biosafety II cabinets and centrifuges (Nuve), inverted and fluorescence microscopes (Leica), microplate readers, cellular imaging and high content screening systems (Molecular Devices - MolDev).
Additionally overall cell culture is supported by a range of bioreactors, whether these are small volume plastic cellready or glass autoclavable bioreactor systems (Getinge Applikon) or bioreactors specifically for adherent cell lines (Pall Biotech - iCELLis and iCELLis Nano) or Xpansion. Suspension cell lines can be supported by single use bioreactor systems (Pall Biotech - XRS Rocking & Stir Tank Reactors - STRs) as well as stainless steel systems with supporting CIP & SIP (Getinge Applikon). Finally single use fermentors can be supported from Cytiva. These systems can range from R&D through to pilot and finally commercial GMP manufacture for therapeutics and biologicals. Fermentation applications can see additional support from our peptone support partner CONDA.
We discuss 3D cell model workflows going into an overview of spheroids and organoids, additionally we review cell engineering-based approaches related to 3D bioprinting with cell inks as well as cell scaffolding and microfluidic (organ on a chip) setups. Major benefits of using 3D models over 2D cell culture is also reviewed.
Core supporting products for 3D cell models includes extrusion-based bioprinters (CELLINK) as well as light-based bioprinters and bio-dispensers, 3D spheroid culture medium (Takara), Live cell analyzers (CYTENA), microplate readers, automated cellular imaging and high content screening systems (Molecular Devices), THUNDER 3D imaging (Leica) as well as STELLARIS confocal and digital light sheet and finally MICA automated cellular imagers.
Next in the discussion we review 3D cell models in cancer research as well as review how multiple technologies come together to support 3D Drug Development pipelines related to target identification & validation, lead generation & refinement, preclinical studies and in vitro studies. All this supported by genomics and proteomics.
We close on vaccine development touching on antigen discovery, antibody discover (phage displace & hybridoma screening) lastly we review factors related to cell line development.
Hosted by Holisha Moodley and Jeff Verlinden from Separations
Phone: [phone] 919 1000 (local)
Email: [e-mail]