Single Cell Sequencing - Eric Chow (UCSF)
ฝัง
- เผยแพร่เมื่อ 2 พ.ค. 2024
- www.ibiology.org/techniques/s...
Dr. Eric Chow gives an overview of single cell sequencing, explains why this approach is useful, and talks through the leading methods.
Single cell sequencing, as the name implies, allows researchers to examine the genomic information for individual cells. This provides an opportunity to examine cell-to-cell differences and identify cell subtypes, which provides insight into how specific cells function within and respond to their environment. Dr. Eric Chow begins his talk with an overview of single cell sequencing with a focus on RNA. He then goes on to outline the predominant approaches, including plate-based, microfluidic-based, and combinatorial indexing methods. He finishes by addressing approaches to single cell analysis that don’t rely on RNA, including methods that use DNA, proteins, and antibodies. He also reviews some of the benefits and limitations of analysis at the level of individual cells.
0:00 Start
0:31 Bulk vs. single cell analogy
4:58 Plate-based SMART-seq
7:08 DropSeq
13:54 Combinatorial Indexing
22:56 Conclusions
Speaker Biography:
Eric Chow is an assistant professor in the Department of Biochemistry and Biophysics and the Director of the Center for Advanced Technology (CAT) at the University of California, San Francisco. The CAT provides resources for UCSF labs wishing to use next generation sequencing techniques and Chow’s research program strives to develop new applications for NGS in pathogen diagnostics. Chow received his BA in molecular biology from the University of California, Berkeley and his PhD in biochemistry from UCSF.
Credits:
Karen Dell (iBiology): Producer
Eric Kornblum (iBiology): Videographer & Video Editor - วิทยาศาสตร์และเทคโนโลยี
Please get Eric to do more of these videos, this is by far the best overview, and easiest to understand, that I’ve found. He’s so good at actually explaining in a way that is not overly technical on purpose. I feel that many in the field are gatekeeping their knowledge, instead of explaining in ways that others can understand.
right....i feel researchers nowadays are reluctant to teach students technical skills and overly focusing on theory and critical thinking....thats why they need to import tons of post docs from china and India because American student are lacking technical skills!! this is really messed up!
Really cool! Thank you Dr. Chow for this detailed and at the same time clearly structured talk!
Excellent talk and lucid explanation of the biology Dr. Chow. Thanks iBiology, looking forward for such talks in future.
Thank you for the nice introduction of single cell sequencing methods!
One of the best scSeq overviews out there. Keep it up you're doing great!
This lecture is highly informative and greatly valuable. It has cleared most of my gray areas in the field of single cell analysis
Dr Chow I love your videos. Please continue your good work. Its really thorough and well explained with theory behind each technique which are not explained from the product maker /companies.
Thank You!
Extemely helpful for newbies!!! Thank you Dr. Chow!!!
Excellent overview summarizing development up to 2019 in this developing field. Thank you for your hard work putting this presentation together!!
Thank you Dr. Eric for this wonderful talk. You have provided detailed information on Single Cell Sequencing and explained every detail of this technique. I found the lecture valuable and highly informative. Thank you very much iBiology Techniques for providing this valuable lecture.
Thank you for this coherent and excellent introduction!
Thank you, It was really good to understand the basics of single cell sequencing methods.
thank you for explaining these technologies so clearly!
it's very useful for me.
Thanks for this amazing video Dr Chow
Bravo! Great video. Single Cell - clearly expained!
Great presentation! Just great! I agree with all of the other positive comments. The content is great, easy to follow and Eric is a great presenter.
17:13 quantifying proteins using DNA sequencing (CITE-Seq)
21:26 universal antibodies for a universal cell-surface protein that have unique barcodes for each cell to detect the unwanted doublets (droplet with one bead but two cells)
Amazing video, amazing explication. Thank you Dr. Chow!
Love Eric’s videos so easy to understand!
These sequencing videos are really great! I hope you can also cover UMIs, spatial transcriptomics, and other such difficult concepts :)
Great video, thank you! Very informative and easy to understand!
Thanks for the explanation! Very helpful for my course
Thank you so much for this lovely talk. I think this is the best talk summarizing scRNAseq.
Fantastic video! You explain very well
Excellent talk
Dr. Chow you is da G.O.A.T. grateful!
Thanks for the insightful sharing.
Thank you Dr. Chow for that! That was explain so well, so much detail but you explained it in a way that was so easy to understand this! Such a great presentation!
Excellent explanation thank you so much!!
nicely explained....
wonderful lecture
Just splendid!!
Fantastic video.
You look for something on TH-cam. The video with Eric Chow pops up. You know its gonna be good...
not one dislike - exactly! Love data visualized in a 3D space!
Thank you for the lecture. I need to go back to school. It would have been useful to include costs/instruments
Brilliant.
thank you!!!!!!!SO MUCH!!!!
Really cool what is the tool you use to record this video that you can embed into the video?
well mam from last few weeks ,I am focusing on biochemistry portion ,althrough I read all content of book but I'm not satisfied with that content , can you please answer or make vedio on my following queries:
(1) in lab whenever we isolate some compound and want know the structure of compound ,how do we really formulate them , what are those techniques , are these techniques 100%accurate or just give approx idea
For example , I always wonder how scientist who has first isolated ATP molecule , how he give structure of ATP , infact how he isolate compound at level of molecule, (since i have isolate compound at level dna only) how cycle such as kreb cycle or glycolysis are performed in lab
Your entire vedio lecture have always motivated me , thanks a lot
I am from india
Great video.
I got a question, In demuxlet. If you are working with different individuals, the possibility of two different cell types from same individual is not that rare. How they differentiated the cell population from same patients.
What's the overall accuracy of Seurat and Scanpy? Is that significant?
What is the influence of clustering on downstream analysis? Differential expressed gene?
I looking for good videos in how analyzing the a dataset of Peripheral Blood Mononuclear Cells (PBMC) ?? Thank you
Can you please explain what microfluidic is?
Thx for watching!
Thank you sir this was very helpful
How's performance of supervised methods? Any pitfall?
Thanks. Can you clarify at 8:04 when you said each beads has many barcoded oligo-dT oligos on its surface. What is the scale of this "many"? Millions? Are these supposed to attach to the entire transcriptome of a cell?
Why can't the doublet microparticles be distinguished from singletons using light scattering or fluorophore techniques?
I'm pleasantly surprised that this video has this many views!
Is clustering the bottleneck of scRNA-Seq or the doublelets. Does 0.6 clustering accuracy mean 0.4 doublelets?
I love your voice.
How do they know which cell type the mRNAs came from originally?
as of 2020, approximately how long does it take to run scRNA-seq in a single sample?
atleast 2 days
@@sofiakathiria7050 thanks, someone told me 1 day and i was very skeptical :)
Some segments in the video are stamped not adjacent to each other
5:00 first cell sequencing,,,
Lost my appetite for smoothies!
How sexy is his voice omg… nearly forgotten what I’m here for.
I think I found my husband!
Not a good lecture, unless you are specialized in this field; even not clear for medical students to understand what is he talking about