SDS PAGE | polyacrylamide gel electrophoresis

SDS PAGE polyacrylamide gel electrophoresis - This lecture explains about the SDS PAGE or polyacrylamide gel electrophoresis procedure technique. Stay tuned to learn more about -
SDS PAGE principle
sds page procedure
sds page application in separating protein mixture.
SDS PAGE is best used to separate proteins based on their molecular weight or size. In this video lecture the working principle and use of sds poly acrylamide gel electrophoresis is explained.
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[@nilaysaha1835]

Thank you very much Sir...these two videos are more than enough to clear our confusion on gel electrophoresis..I wish to know more about Gradient Gel Electrophoresis from you...also watched your lecture on 2D gel electrophoresis...that was also very much interesting...Honestly speaking Sir...you are an Encyclopedia to us...on all these topics on Biology

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Yesterday my teacher expalined this topic but i am confused many time so just search about electrophoresis and your video is found......thank you for helping to understanding in this topic

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[@rashiyadav7017]

Great video! I have a question- in one of my experiment, I noticed that a diner protein (44kda) was migrating slower and was found at 50kd. This dimer has positively charged amino acids. Can you please tell me what could be the possible reason for slow migration of this protein?

[@ZeroSectionStyle]

Hey! Always thankful for your stuff, incredibly educative. I have a question. Ive been studying 2DGE lately, and my professor always stresses that you need to prepare the sample for the MW based separation with iodoacetamide to remove S-S bonds, but this seems to do the same thing as B-mercaptoethanol, does anyone know why doing both of these things is important, and why Iodoacetamide does not suffice on its own? Thanks!

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Ok sir

[@paramedical8133]

Thanks sir..for explaining each and every key points🤗

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Thanks a lot man, you are a great teacher. This is now really clear.

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[@atharvakulkarni8818]

Sir i like your videos so much.I like thid one too.....i was unable to understand SDS -PAGE . I search for book too but did not find out.but your video helped mee alot!....pls also make video on detailed applications of modern technquies.....it will be so kuch helpful.🙂🙂🙂

[@shomusbiologyofficial]

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[@abhishekbhandary3693]

Sir I had one doubt
In SDS page all the proteins get negative charge from the SDS so that they can easily move towards anode, but in native page there is no charge donator like SDS then how positively charged proteins gets seperated?

[@ruwanihettiarachchi305]

Thank you dear Sir! Well explained everything 👍

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[@kavyap4453]

Thank you for making my concept on sds-page very clear 😃👍👌

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[@abhijitaher1515]

Also I have one more question that as we use tris glycine buffer as running gel in sds. The glycine enters the gel and help in lining of the proteins before entering the resolving gel. Is it possible to run sds if we don't use glycine.

[@raenashaikh3377]

This was really detailed and helpful... thanks a lot sir!!

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You're welcome

[@mdrezoanulislamfahim1268]

Thank you verry much ❤️❤️ from Bangladesh

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It's very helpful to my undergraduate studies. Thank u so much sir

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[@jayeetachowdhury970]

Sir this vedio is very helpful for me... I hope you'll make a vedio on 2D Gel electrophoresis soon.. 🙂

[@shomusbiologyofficial]

Sure

[@wideworldofscience1721]

Great method. Of explanation

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Best way to teach

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Wow sir...🔥 awesome explanation

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Superb 😀 by the way with explanation ur also looking nice sir

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Thank you

[@FatmaAli-tb8tq]

Thank you but can you please state the reason behind using separate gels (stacking and resolving)?

[@Amitkumar-ro3re]

Just awesome..

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Thank u sir
It is really helpful..

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Thanks bro

[@bscool123]

Separation take place on the basis of, because all particle have approximately the different charges , and different masses ???? Please tell me

[@mahakehail8425]

Can we separate RNA by Agaros gel also?
And if we have mixture of DNA snd mixture of RNA in gel, who will being faster? DNA or RNA
Or we not compirsion DNA and RNA togather?
Just DNA size it self and RNA itself?

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[@priyobratasinha7300]

2.5 mAmp current is required in case of semidry transfer. Not in case of SDS-PAGE. Rather after SDS-PAGE the 2.5 mAmp current is used to transfer the proteins from gel to the nitrocellulose or PVDF membrane in case of semidry transfer process only.

[@divyasree3329]

Well understood somu sir

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Thank you

[@tejureddy5474]

How much of voltage is sufficient to run an SDS PAGE to get a protein band of molecular weight 27kDa

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Respect for u

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Thank you

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Great sir....

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Thanku sir

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Nyc sir very helpful 😊

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thankyou sir for this video

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You're welcome

[@neetladder]

Why we get four bands for BSA in SDS PAGE? If you have an proper explanation then please help me?

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Thank u sir

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Nice teaching sir.

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Best video

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Thanks sir..

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[@gayathrigaganan202]

Sir, which amino acid interfere with the SDS page?

[@vidushisoam1395]

Sir, you have not told about the stacking and separating gel difference..

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Thank you so much

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You're welcome

[@lavleshmishra8846]

Helpful 😊

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You're welcome

[@idealexotic4072]

I have one question what is denaturing polyacrylamide gel electrophoresis and how is this different from native page or SDS page?

[@shomusbiologyofficial]

In native gel, we don't denatured the protein. It moves as it is in structure and in denatured gel, we denatured proteins and then allow it to migrate in gel

[@shivangi_1223]

Well explained

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Thank you

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Love you sir

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Thank you

[@rohitsutar4981]

Why SDS give 2 band in antibody separation?? Plz ans...

[@vasantharumugam602]

very nice explanation sir thank you so much... Sir can I say this video about one-dimensional gel electrophoresis

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[@rakshajain43]

How many polypeptide bands detected on gel, if we electrophorised secretory IgA on SDS-PAGE under reduced and denaturing conditions. Sir, plz answer urgently 😔😔😔😔

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New ones

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Thank you❤️❤️

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[@anindita2169]

in sds two types of gel are present one is stacking gel another is resolving gel. it would be much more of help if you have included this as well

[@srividhyanarayanan7337]

I was looking for it actually

[@SambitPatra1]

SDS attach to which amino acids of protein??

[@sakshisingh8954]

Is SDS-PAGE is horizontal type of electrophoresis??

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I will consider it. Thank you

[@ankitaroy7153]

Sir really really thank you so much 😊
Sir mujhe 1 Question puchhna h
SDS-PAGE m do Ph Q lete h Resolving Gel & Stacking Gel k lie ?
Please sir please please reply with d answer.
I have to answer this
Please sir

[@ashishkumarsingh007]

Which buffer is used?

[@namratasingh4161]

Nice explained this topic. Sir please suggest some books for research methodology if possible

[@shomusbiologyofficial]

Coming soon

[@namratasingh4161]

Thank you so much

[@paramedical8133]

Sir,why we need to separate the protein...clinical significance???

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Thanks

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[@souvikhajra4213]

Sir where Resolving gel and stacking gel?

[@Wgh552]

Thanku sir s much

[@shomusbiologyofficial]

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[@ahelimukherjee3777]

Can you please discuss on the mathematical problems based on SDS Page and ESI-MS? Like the ones asking for molecular weight of the protein?

[@priyankasahoo3087]

Can you please make videos on molecular biology experiments? Such as DNA isolation, protein estimation, protein purification

[@shomusbiologyofficial]

Okay

[@abhijitaher1515]

Sir as you have taught about SDS-page here we have studied that in sds page we use 2 gel that is resolving gel and stacking gel. Is it possible that we perform SDS- page in a single whole gel.

[@shomusbiologyofficial]

Both type of gel needed for better resolution

[@abhijitaher1515]

@@shomusbiologyofficial sir then can we do sds in single conc of gel eg if resolving gel has 12% acrylamide then we use the same conc in the whole gel

[@abhijitaher1515]

In what conditions we can use single conc gel

[@wardasarfraz5198]

cathode is positively charged and anode is negatively charged, but here you said fragments will ,ove from cathode to anode, can you clear it?

[@shomusbiologyofficial]

Anode is positive and cathode is negative. Easy way to remember is "Don't PANIC"

[@pabitradebnath1917]

Function of SDS is now clear.

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Guys let's support shomu's biology

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Thank you

[@prajaktaharmalkar7952]

SDS-PAGE is also a discontinuous gel system made up of two different pH gels called stacking gel of pH 6.8 and separating gel of pH 8.8 which also differ in its pore size. You have not mentioned this important point

[@shomusbiologyofficial]

Thank you for mentioning

[@prajaktaharmalkar7952]

@@shomusbiologyofficial Thank you sir. Also thank you for such easy and well explained videos.

[@krupalimakwana5941]

What is stacking and separating gels in SDS page ???

[@randbalata2950]

Non-science students will be scared from just the full name of this test 😁 we rock!

[@shomusbiologyofficial]

🤔

[@nknk-xo2qx]

Sir plz as soon as possible make vid on gradient gel

[@shomusbiologyofficial]

Okay

[@shradhamahendra3958]

Your videos are good but not complete information is provided