SDS PAGE gel electrophoresis | sds page principle explained | poly acrylamide gel electrophoresis

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  • เผยแพร่เมื่อ 15 ธ.ค. 2024

ความคิดเห็น • 44

  • @abigailamusan9775
    @abigailamusan9775 ปีที่แล้ว +14

    Once again, sir, you're saving my grades. Thank you 🥺

  • @mahmodahaque28
    @mahmodahaque28 8 หลายเดือนก่อน +10

    Sir much obliged from Bangladesh,
    I am a first year student of Biochemistry and Molecular biology, Dhaka university

  • @namkook9515
    @namkook9515 5 หลายเดือนก่อน +1

    Sir thank you so much u always save me just before my exams. thank u so much

    • @shomusbiologyofficial
      @shomusbiologyofficial  5 หลายเดือนก่อน

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @ChetanyaPal-lp1id
    @ChetanyaPal-lp1id 9 หลายเดือนก่อน +5

    Sir my professor makes his lectures from your videos 🙏

    • @shomusbiologyofficial
      @shomusbiologyofficial  9 หลายเดือนก่อน +4

      Many people do. Helping teachers since a decade

  • @talibmalik2616
    @talibmalik2616 ปีที่แล้ว +2

    Thanks sir.u make it so easy ..

  • @layannnnnnnnnnnnnnnnmm
    @layannnnnnnnnnnnnnnnmm 10 หลายเดือนก่อน +2

    You are a king 👑👑👑👑👑

    • @shomusbiologyofficial
      @shomusbiologyofficial  10 หลายเดือนก่อน +1

      Thank you so much for appreciating my efforts

  • @pallobbarai4152
    @pallobbarai4152 7 หลายเดือนก่อน +2

    Thank you sir, very helpful🙏🙏

  • @MinaMahmoudi-t5o
    @MinaMahmoudi-t5o 9 หลายเดือนก่อน +1

    Best as always 😍

  • @vijayatalari5980
    @vijayatalari5980 ปีที่แล้ว +4

    Hlo Sir.
    Your lectures are very useful to us. I got more knowledge from your's only sir.
    Thanking you.

  • @rhythmonfire1.0
    @rhythmonfire1.0 9 หลายเดือนก่อน +1

    Thank you for lecture sir

  • @CHANDANDAS-xb3pt
    @CHANDANDAS-xb3pt ปีที่แล้ว +4

    Thank you sir for this detailed explanation. I just had one query . Sir as the interaction between SDS and amino acids is that there is one SDS between two amino acids. Now, as SDS gives the negative charge, shorter polypeptides or proteins having low molecular weight will be less negatively charged, as they've less number of amino acids . In this case the lengthier polypeptides have more negative charge ..so in that case they should migrate faster towards the anode . Sir, does this parameter exert any influence in the separation process ....🙏

    • @avanimirchandani8190
      @avanimirchandani8190 10 หลายเดือนก่อน

      In SDS-PAGE, the movement of proteins is primarily based on their size, not their charge. SDS denatures the proteins and binds to them, giving all proteins a negative charge and essentially neutralizing any charge-based differences between proteins. Therefore, the speed at which a protein moves through the gel is primarily determined by its size, with smaller proteins moving faster and larger proteins moving more slowly. This is why the technique is used to separate proteins based on their molecular weight.

    • @Bank-al-habib-biometric-soluti
      @Bank-al-habib-biometric-soluti 8 หลายเดือนก่อน +1

      In SDS-PAGE, while it's true that larger proteins will have more SDS bound to them, resulting in a greater negative charge per protein molecule, the overall effect is that the charge-to-mass ratio becomes relatively constant for all proteins. This is because the mass of the protein also increases as it gets larger. As a result, the mobility of the proteins through the gel primarily depends on their size, not on their net charge.
      So, larger proteins will indeed have more negative charge, but they also have a larger mass, which balances out the effect on mobility. Therefore, in SDS-PAGE, smaller proteins typically migrate faster through the gel because they encounter less resistance, while larger proteins migrate more slowly due to their greater size.

    • @CHANDANDAS-xb3pt
      @CHANDANDAS-xb3pt 7 หลายเดือนก่อน

      @@avanimirchandani8190 Thank you so much ... 🙇🌸

    • @CHANDANDAS-xb3pt
      @CHANDANDAS-xb3pt 7 หลายเดือนก่อน

      @@Bank-al-habib-biometric-soluti Thank you so much 🙇🌸

  • @BOTANIKOSPM
    @BOTANIKOSPM ปีที่แล้ว +2

    Nice presentation

  • @foodie0080
    @foodie0080 7 หลายเดือนก่อน +2

    Sir you haven't explained about resolving and stacking gel ..

  • @Afrin2005
    @Afrin2005 2 หลายเดือนก่อน +1

    Thanks

    • @shomusbiologyofficial
      @shomusbiologyofficial  2 หลายเดือนก่อน

      You're welcome

    • @Afrin2005
      @Afrin2005 2 หลายเดือนก่อน

      @@shomusbiologyofficial sir can you make a video about bicarbonate buffer system.

  • @BrotherThamue
    @BrotherThamue ปีที่แล้ว

    Thank you very much Sir

  • @saquibhaider377
    @saquibhaider377 ปีที่แล้ว

    Sir when gel formation two type of phase stacking and separating....

  • @ankitabanerjee2188
    @ankitabanerjee2188 ปีที่แล้ว

    very helpful video.
    Sir, can you make a video of how to solve part-C question from this topic? would be really helpful🙏

  • @sarwarmedia2833
    @sarwarmedia2833 ปีที่แล้ว

    why protein have di-sulphate linkage ? pls
    explain it? enybody

    • @isha8349
      @isha8349 ปีที่แล้ว

      CERTAIN AMINOACID HAVE S PRESENT IN THEIR STRUCTURE WHICH MAKES S-S LINKAGE

  • @fatmaelgedawy2061
    @fatmaelgedawy2061 ปีที่แล้ว +2

    Sir I need real time pcr
    Please I have an exam😢

  • @fooballers7883
    @fooballers7883 10 หลายเดือนก่อน

    So for lipoproteins will have the same mechanism of action ?

  • @VidhibaZala-gm8te
    @VidhibaZala-gm8te 11 หลายเดือนก่อน +1

    Thank you very much sir 😊