SDS-Polyacrylamide Gel Electrophoresis

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  • เผยแพร่เมื่อ 5 ม.ค. 2025

ความคิดเห็น • 149

  • @felixg4785
    @felixg4785 9 ปีที่แล้ว +269

    this guy needs to be cloned and teach every single BIO course.

    • @Lejonet120
      @Lejonet120 8 ปีที่แล้ว +5

      doont need to clone when we can have youtube. Every single Bio course can be taken here :)

    • @janeijama8564
      @janeijama8564 7 ปีที่แล้ว

      Felipee Rincon I concur 😊

    • @dickensochieng3256
      @dickensochieng3256 2 ปีที่แล้ว

      🤣 "should be cloned"🤣

  • @naqiyahmulachelah5668
    @naqiyahmulachelah5668 5 ปีที่แล้ว +13

    for the past 4 years, your channel have helped me through my hard times until i finally got my degree. Now look, this is my first time working and i'm still here. i still need you. THANK YOU MISTER!!! i hope you have a happy life! now and always!

  • @Rivamus
    @Rivamus 9 ปีที่แล้ว +18

    You explained this better than both my professor and textbook. I look forward to watching your other videos!

  • @jonvonstein3390
    @jonvonstein3390 9 ปีที่แล้ว +39

    I love how in depth you you go but are still easy to follow. Thank you!

    • @shaki6500
      @shaki6500 5 ปีที่แล้ว +4

      exactly, most khan academy or other resources give you a very shallow info and you get more and more confuse, but he breaks down everything

  • @beneastman9244
    @beneastman9244 5 ปีที่แล้ว +3

    Incredible! This guy is always giving the most helpful, concise information out there. Never a syllable wasted

  • @malihamehnaz1840
    @malihamehnaz1840 ปีที่แล้ว

    Becoming a fan of yours day by day. Not everyone has this capability of making people understand complicated topics. Thank you so much

  • @rebecavelasquez2910
    @rebecavelasquez2910 5 ปีที่แล้ว +2

    This was a topic that could have been confusing and hard to understand but you managed to explain it clearly and it was easy to follow your explanation! Great Job!

  • @firaca100
    @firaca100 4 ปีที่แล้ว +3

    Very useful! You explain another points of view from this topic that I don't really usually found in textbook or in our lectures. Thanks a lot!

  • @maitreyeehira3463
    @maitreyeehira3463 5 ปีที่แล้ว

    I probably never get the chance to tell you in person that YOUR lectures are the main reason of my GPA =8 in my mater degree...YOU are just awesome man......

  • @valeriemel4887
    @valeriemel4887 6 ปีที่แล้ว +1

    The only way Im getting through BioChem right now are these videos. Thank you so much!

  • @zahraammadani
    @zahraammadani 5 ปีที่แล้ว +1

    The greatest teacher on TH-cam! Thank you so much !!

  • @IdahoWilliams
    @IdahoWilliams 10 ปีที่แล้ว +21

    love the clear diagrams and explanation, thanks!

    • @AKLECTURES
      @AKLECTURES  10 ปีที่แล้ว +2

      needfire you're welcome!

  • @debolinaa5235
    @debolinaa5235 2 ปีที่แล้ว

    Literally you're the best ...your way of explaining is making the concept crystal clear... thank you AK lecture sir

  • @eslamomran899
    @eslamomran899 9 ปีที่แล้ว +4

    You rock..very simplified and clear explanation. Thank you for your efforts

  • @junczhang
    @junczhang 8 ปีที่แล้ว +9

    definitely the best videos on youtube!! Better than Khan academy!

  • @sserwaddaderrick1523
    @sserwaddaderrick1523 7 หลายเดือนก่อน

    Masters level ❤:: this is my teacher for my masters class

  • @PrimeValar
    @PrimeValar 3 ปีที่แล้ว

    Honestly man, how are you so good??

  • @AdesomojuEsther-mq4xy
    @AdesomojuEsther-mq4xy 9 หลายเดือนก่อน

    More strength Mr andrey ,your lecturers are helpful

  • @保禄-d9c
    @保禄-d9c 2 ปีที่แล้ว +1

    about F=Fv:Because friction is more complicated, it is usually some empirical formula. In the past, the friction force you were in contact with usually referred to dry friction between solids. If you consider that the object is moving in air or liquid, the measurement will find that the friction force is related to the speed. It is generally considered that at high speed, the friction force is proportional to the square of the speed, and when moving slowly, the friction force is approximately linear with the speed.

  • @louis-charlesdesjardins688
    @louis-charlesdesjardins688 6 ปีที่แล้ว +1

    Thank you for making all my first biochemistry experiments easier.

  • @israaomar9487
    @israaomar9487 5 ปีที่แล้ว

    Thank you so much i just scored a full mark in my final exam because of your lecture God bless you

  • @HA-wn3gg
    @HA-wn3gg 8 ปีที่แล้ว +3

    beyond PERFECT

    • @AtifKhan-kg3qj
      @AtifKhan-kg3qj 3 ปีที่แล้ว

      Did you get that A+?

    • @HA-wn3gg
      @HA-wn3gg ปีที่แล้ว

      @@AtifKhan-kg3qj Yes I did !!!! Now I'm doing my MSc and imagining I'm watching his videos AGAIN!!!

  • @donut_eeba
    @donut_eeba ปีที่แล้ว

    I mean he is always slaying ❤❤❤❤❤❤❤

  • @linahebi7525
    @linahebi7525 7 ปีที่แล้ว +1

    You explain so good, it's just amazing. I understand you better that the same theme in my mother-language :))

  • @MrsHarryPotterFan97
    @MrsHarryPotterFan97 7 ปีที่แล้ว

    I have been following you for years and really I am so grateful, thanks sooooo much :D Your videos are always so clear and complete

  • @malikbasharat7119
    @malikbasharat7119 9 ปีที่แล้ว

    No wordss for ua teaching....gets stored in brain

  • @Reree-gz5bg
    @Reree-gz5bg 4 ปีที่แล้ว +2

    you do such a great job. thank you XD. I had a few friends who took biochem (lecture and lab) and strongly recommend watching you

  • @賴羿嘉-u7z
    @賴羿嘉-u7z ปีที่แล้ว

    Fantastic explanation🎉🎉❤

  • @akankshasingh3220
    @akankshasingh3220 4 ปีที่แล้ว

    Loved the way you explain..every point. thanks for explaining it 👍 so well

  • @danieleorlandoni4164
    @danieleorlandoni4164 8 ปีที่แล้ว +1

    You could teach biology even to an alien! great job buddy!

  • @nunitchagucci3047
    @nunitchagucci3047 7 ปีที่แล้ว

    Your channel is such a wonderful learning resource. Thank you so much!!!

  • @drharikartha
    @drharikartha 9 ปีที่แล้ว

    one of the beautiful lectures i listened..thanks dear andrey

    • @AKLECTURES
      @AKLECTURES  9 ปีที่แล้ว

      Drhari Kartha Thanks a ton! :)

  • @abbierieder5807
    @abbierieder5807 3 ปีที่แล้ว +1

    How is the equation F_f = f*v derived? I know F_f = mu*Fn but not sure how you're getting that derivation.

  • @fatmamahmoud2689
    @fatmamahmoud2689 9 ปีที่แล้ว

    Thank you for the simple explanation and the clear diagrams. I was in a bad need to understand that type of gel electrophoresis.

  • @lisatouyon1456
    @lisatouyon1456 9 ปีที่แล้ว +1

    your lecture is so on point. Thank you so much for being helpful to me!

    • @AKLECTURES
      @AKLECTURES  9 ปีที่แล้ว

      Lisa Touyon you're welcome Lisa, glad to be of help :)

  • @rahaffaqeeh7769
    @rahaffaqeeh7769 4 ปีที่แล้ว +1

    My uni has to pay for you , Thank you!

  • @evelynmoyosithole1863
    @evelynmoyosithole1863 2 ปีที่แล้ว

    "In just a moment" .... Thank you so much

  • @kathypychova1137
    @kathypychova1137 9 ปีที่แล้ว

    thank you, finally understood physics :D and ofcourse SDS-PAGE... your lectures are great!

    • @AKLECTURES
      @AKLECTURES  9 ปีที่แล้ว

      +Kathy Pychova you're welcome Kathy :)

  • @vantran-bk1tn
    @vantran-bk1tn 8 ปีที่แล้ว +1

    you are great lecture! It is clear to understand. Thank you so much!

  • @rotemamirkeret3946
    @rotemamirkeret3946 9 ปีที่แล้ว

    your explanation is so clear. thank you.

  • @PillsHere222
    @PillsHere222 2 ปีที่แล้ว

    Not be a nerd, but SDS is missing one more carbon in the video (Dodecyl = 12). I only see 11 carbons in the drawing. I appreciate your work, and needed this very much.

  • @zheidari001
    @zheidari001 7 ปีที่แล้ว

    You are the best, very helpful clips

  • @BBC932
    @BBC932 9 ปีที่แล้ว

    That is a very good explanation. Many thanks

  • @carlypenaemprende
    @carlypenaemprende 7 ปีที่แล้ว

    The best explanation EVER! Thank you :)

  • @weakcandlelight7793
    @weakcandlelight7793 10 ปีที่แล้ว +2

    great video! Please continue to make more.

  • @samanthawinters8263
    @samanthawinters8263 8 ปีที่แล้ว +1

    I like you, all your videos are so thorough! thank you so much

    • @AKLECTURES
      @AKLECTURES  8 ปีที่แล้ว

      Thanks Samantha!

  • @alexrytikoff9627
    @alexrytikoff9627 9 ปีที่แล้ว

    Great explanation. Little review of physics was helpful

    • @AKLECTURES
      @AKLECTURES  9 ปีที่แล้ว

      Alex Rytikoff Thanks Alex, always like to use physics when applicable! :-)

    • @tayzarlinn7619
      @tayzarlinn7619 9 ปีที่แล้ว

      AK LECTURESplease show me the link of denatured polyacrylamide gel DNA .

    • @AKLECTURES
      @AKLECTURES  9 ปีที่แล้ว

      tay zar linn google it.

  • @cristinavall8587
    @cristinavall8587 5 ปีที่แล้ว

    You are amazing! Thanks for your explanations

  • @chagondagodfrey3576
    @chagondagodfrey3576 5 ปีที่แล้ว

    what a good presentation. keep it up

  • @bacanimichael
    @bacanimichael 4 ปีที่แล้ว

    great explanation

  • @lauradumo
    @lauradumo 7 ปีที่แล้ว

    Best explanation ever, thank you so so much!

  • @jhondelv8891
    @jhondelv8891 7 ปีที่แล้ว

    Overall good lecture....... 8:20

  • @raghadhammami5364
    @raghadhammami5364 2 ปีที่แล้ว

    Accidentally today is the birthday of this video 😂

  • @nevtonteixeira4723
    @nevtonteixeira4723 4 ปีที่แล้ว

    Excellent class!!

  • @qusaisyr6094
    @qusaisyr6094 5 ปีที่แล้ว

    you are the best , you should be teaching in Harvard

  • @milanbhattarai3396
    @milanbhattarai3396 3 ปีที่แล้ว

    You're the best.

  • @suchizinzuwadia3527
    @suchizinzuwadia3527 9 ปีที่แล้ว

    Thank you SO much! Your videos are so helpful!!!

  • @mohamedchanfioumkouboi6443
    @mohamedchanfioumkouboi6443 8 ปีที่แล้ว

    Very beneficial lecture. Thanks so much....

  • @Artas1984
    @Artas1984 5 ปีที่แล้ว

    He is right about the electric field being constant, BUT! Actually the voltage is not constant! In order to maintain a constant electric current, which generates ''almost'' constant velocity, the gel-electrophoresis machine increases the voltage during the process in order to resist the ever increasing impedance values inside the medium (anyone doing gel-electrophoresis can see that). Actually during the migration the voltage more than doubles! Does not matter in the end... Excellent video anyway!

  • @kikiignatiadis9212
    @kikiignatiadis9212 8 ปีที่แล้ว

    This was informative and awesome. Thank you!

  • @ChuiBeat
    @ChuiBeat 8 ปีที่แล้ว

    Great explanation!

  • @lovelyyarora6207
    @lovelyyarora6207 9 ปีที่แล้ว

    juzzz in luv wid ur lectures ...(y) !! brilliant !! :)

    • @AKLECTURES
      @AKLECTURES  9 ปีที่แล้ว

      lovelyy arora thank you! :-)

  • @ikramullah5189
    @ikramullah5189 8 ปีที่แล้ว

    simply outstanding

  • @Asmaafahmey
    @Asmaafahmey 9 ปีที่แล้ว

    your videos always help me, but I only need you to zoom in the board, so tht we can see whts written

  • @saieshwar7431
    @saieshwar7431 ปีที่แล้ว

    Hands off

  • @keerthikailath2053
    @keerthikailath2053 2 ปีที่แล้ว

    thank you for this video.

  • @fbegum1738
    @fbegum1738 9 ปีที่แล้ว

    brilliant lecture!

    • @AKLECTURES
      @AKLECTURES  9 ปีที่แล้ว

      f begum thanks! :)

  • @pand100
    @pand100 7 ปีที่แล้ว

    Excellent! Well done!

  • @moririn9923
    @moririn9923 3 ปีที่แล้ว

    can we obtain the separated protein from the gel

  • @DiscoSupernova
    @DiscoSupernova 5 ปีที่แล้ว

    Dope lecture

  • @amirhosseinshafieian3951
    @amirhosseinshafieian3951 3 ปีที่แล้ว

    I think there is a problem, the coefficient of friction must be without dimension, while here you when you multiply f with velocity it does not make sense, would please clarify it???!!!!!

  • @adiga202
    @adiga202 9 ปีที่แล้ว +7

    just what i needed ! thank you very much !! ^_^

    • @AKLECTURES
      @AKLECTURES  9 ปีที่แล้ว +2

      adiga202 You're very welcome! :)

  • @johnwaynard1506
    @johnwaynard1506 8 ปีที่แล้ว

    It occurs to me that the structure formula of SDS written on the white board is incorrect (one "CH2" is missing in the dodecyl fatty acid)...
    But otherwise: great explaination!

  • @Soriyou3
    @Soriyou3 7 ปีที่แล้ว

    Thanks for a great video. I have one question. How is the force of friction = f*velocity? Isn't frictional coefficient dimensionless so the unit does not come out to be Newtons?

  • @sofiastar9197
    @sofiastar9197 5 ปีที่แล้ว

    Thank you very much ...

  • @trinaroychoudhury3443
    @trinaroychoudhury3443 8 ปีที่แล้ว

    totally awesome..thnks very much..

  • @maxwellnjati1756
    @maxwellnjati1756 10 หลายเดือนก่อน

    The friction comes from the gel?

  • @batuhan4668
    @batuhan4668 3 ปีที่แล้ว

    Great! Thanks!

  • @junyoun4470
    @junyoun4470 5 ปีที่แล้ว

    Can anyone please explain Why frictional force is => frictional constant(f) X velocity (v)? Far as i know Frictional force is equal to frictional constant(f) X Normal Force (N)

  • @Young_Carry
    @Young_Carry 2 ปีที่แล้ว

    You are the god

  • @stephanieangela470
    @stephanieangela470 6 ปีที่แล้ว

    Hi I'm using potassium sulphate buffer in my sample. can it works with it or does it has to be tris HCl? thank you

  • @mahermohammed7448
    @mahermohammed7448 9 ปีที่แล้ว

    plz i have a question , would you tell me the nature of denaturing take place by SDS on protein which doesnt effect on the conformation of protein

  • @ProfoundAscension
    @ProfoundAscension 9 ปีที่แล้ว +1

    Great video, you are very easy to understand. Does SDS page disrupt disulfide bonds?

    • @jonvonstein3390
      @jonvonstein3390 9 ปีที่แล้ว +1

      +M Abdul You are breaking the sulfide bonds with DTT, BME, or TCEP before you put it in the well.

  • @deepikadav
    @deepikadav 8 ปีที่แล้ว

    understood it very well in just the moment :D :)

  • @Ala22223
    @Ala22223 6 ปีที่แล้ว

    your the best

  • @andreabanda3605
    @andreabanda3605 7 ปีที่แล้ว

    Can someone explain how I can use this procedure to determine which membrane proteins are integral vs peripheral?

  • @lourdeskottakali434
    @lourdeskottakali434 4 ปีที่แล้ว

    Thankyou

  • @khaledcampa9593
    @khaledcampa9593 8 ปีที่แล้ว

    Thank you!

  • @violinsheetmusicblog
    @violinsheetmusicblog 8 ปีที่แล้ว

    Is the charge-to-friction ratio the same thing as the charge-to-mass ratio?

    • @babakrahimi3914
      @babakrahimi3914 8 ปีที่แล้ว

      i dont know how can i say u r the best

  • @ridsbutterfly9850
    @ridsbutterfly9850 8 ปีที่แล้ว

    Thanks a lot Sir..

  • @Gooch_cruiser
    @Gooch_cruiser 7 ปีที่แล้ว

    I always thought the reason ads page was due to size not charge was because the charge to mass ratio for all proteins is the same with SDS.
    10 amino acids = every other amino acid gets sds/ = arbitrary net charge of 5. Divide that by 10 = 1/2.
    30 amino acid... 15 charge.. 15/30 is 1/2 .Thus all proteins have the same exact charge/mass ratio.

    • @SforScience
      @SforScience 7 ปีที่แล้ว

      sds add charge uniformly to each and every amino acid so that they can separate based on their charge ratio

  • @abomashhoor583
    @abomashhoor583 9 ปีที่แล้ว

    amazinnnnng. can i ask a tow question: 1. whats the deference between 6x and 2x loading dye and which i should use ?
    2. which voltage is optimal to run one caste of gel, i usually use 65 v. ?

    • @GabrielThomassen
      @GabrielThomassen 9 ปีที่แล้ว

      the concentrations of chemicals in the 6x loading buffer is three times as high compared tot the 2x loading buffer. This is the only difference. You should dilute your protein sample with the loading buffer in such a way that you always end up with 1x loading buffer. the 2x loading buffer should be diluted 1:1 with your sample. the 6x should be diluted 1:5.

    • @GabrielThomassen
      @GabrielThomassen 9 ปีที่แล้ว

      the concentrations of chemicals in the 6x loading buffer is three times as high compared tot the 2x loading buffer. This is the only difference. You should dilute your protein sample with the loading buffer in such a way that you always end up with 1x loading buffer. the 2x loading buffer should be diluted 1:1 with your sample. the 6x should be diluted 1:5.

  • @simonailizarov3769
    @simonailizarov3769 9 ปีที่แล้ว

    thank you so much for these videos!!!!!! thank you thank you thank you thank you.... oh yeah... THANK YOU :)

  • @Wanderer789-v8o
    @Wanderer789-v8o 7 ปีที่แล้ว +2

    What is the role of mercaptoethanol???. Plz reply

    • @menassies3224
      @menassies3224 7 ปีที่แล้ว

      Farida Chy the presence of mercaptoethanol disrupts the disulfide bridge bond found in the protein when mercaptoethanol is not present the disulfide bond in protiens stay intact hope that was helpful

    • @lilna7444
      @lilna7444 6 ปีที่แล้ว +2

      Mercaptoethanol is a reducing agent that cuts disulfide bonds within proteins in order to disrupt the 3-dimensional shape of proteins, a process that is called "denaturation".

  • @hanglee1385
    @hanglee1385 7 ปีที่แล้ว

    Everyone, please answer me, should be running how much mA (current) with mini-gel?

  • @Kebinwmiles
    @Kebinwmiles 9 ปีที่แล้ว

    I find your explantation of the net force on the proteins in the gel to be problematic. Could you direct me to the source of your explanation so that I can review it?

    • @AKLECTURES
      @AKLECTURES  9 ปีที่แล้ว +1

      +Kevin Miles There is no source.

    • @violinsheetmusicblog
      @violinsheetmusicblog 8 ปีที่แล้ว

      How did you know that the frictional force = frictional coefficient * velocity? I have never seen that equation before.

  • @aditilad3173
    @aditilad3173 6 ปีที่แล้ว

    Please make a video on Native page

  • @lirenseparediano3504
    @lirenseparediano3504 8 ปีที่แล้ว

    Try to explain in many languages, for example in spanish... Nice videos !!

    • @Lejonet120
      @Lejonet120 8 ปีที่แล้ว

      try to teach enlish :)

  • @moishie20
    @moishie20 8 ปีที่แล้ว

    thanks men

  • @namra12345
    @namra12345 6 ปีที่แล้ว

    Thanks a lot sir