I'm glad you liked the video and thanks for the question! Do you mean where does one find reference sequences to compare with? Reference sequences can be found either on NCBI's GenBank (www.ncbi.nlm.nih.gov/genbank/) via the BLAST search functionality (blast.ncbi.nlm.nih.gov/Blast.cgi ), or on BOLD (www.boldsystems.org/index.php/IDS_OpenIdEngine).
Yes, you could certainly produce PCR amplicons for DNA barcoding a whole range of invertebrates. Check out our page on DNA barcoding here: bento.bio/protocol/dna-barcoding/, and animal primers here (they should work on most invertebrates): bento.bio/product/animal-metazoa-dna-barcoding-primers/. You could then send off the PCR amplicons to a Sanger sequence service, check the returned sequences for quality, and then use them to search against the BOLD database (www.boldsystems.org/index.php/IDS_OpenIdEngine) to get a match. I hope that's useful!
That was really interesting Dr Jennifer. Thank you!
Thanks! Glad you enjoyed it ☺️
Very Good.
Where does one obtain the coding region information for each tissue type or species type if you will?
Unknown starting point I mean.
I'm glad you liked the video and thanks for the question! Do you mean where does one find reference sequences to compare with? Reference sequences can be found either on NCBI's GenBank (www.ncbi.nlm.nih.gov/genbank/) via the BLAST search functionality (blast.ncbi.nlm.nih.gov/Blast.cgi ), or on BOLD (www.boldsystems.org/index.php/IDS_OpenIdEngine).
Can this be used to amplify DNA from unknown bugs found in the house for sequencing to identify bug species too?
Yes, you could certainly produce PCR amplicons for DNA barcoding a whole range of invertebrates. Check out our page on DNA barcoding here: bento.bio/protocol/dna-barcoding/, and animal primers here (they should work on most invertebrates): bento.bio/product/animal-metazoa-dna-barcoding-primers/. You could then send off the PCR amplicons to a Sanger sequence service, check the returned sequences for quality, and then use them to search against the BOLD database (www.boldsystems.org/index.php/IDS_OpenIdEngine) to get a match. I hope that's useful!