My first thought when I saw the image of the last plant was that it was Dracaena Sanderiana aka "Lucky Bamboo". So I think your matK result came close, but may not have determined the exact species.
Use a CTAB DNA extraction, then use a modern(!) ITS2 primer and you will get to species level much more reliably. Don't forget DMSO in your PCR if using ITS2. Check the date on the rbcL+matK paper, it's outdated.
Good day, am Kceee. Like your honesty in your report. Am working on something similar. I used rbcL and ITS to barcode 7 plants. I want to compare the results to establish which is a better barcode. Could you give me a guide on some analysis I could do.
Thanks for the feedback 😊 Have you got results from both rbcL and ITS for all 7 plants, and are the sequencing results different? We have a guide on our website that covers the protocols Jenny uses in this video, and works for both rbcL and ITS barcoding bento.bio/protocol/dna-barcoding/
@@BentoLab Yes I have. Please could you advise me on how to analyze the interspecific and intraspecific divergence and also check for barcoding gap. From different literatures I have gone through, these methods were used to determine which barcode marker is best for a particular plant family. Thanks ahead.😊
@@kelechiejindu5487 That goes beyond our scope of bioinformatics knowledge on our team at the moment. Sorry we can't help more - good luck with the project!
Hi, you can find us at bento.bio, and you can check out our DNA barcoding pages on our website here: bento.bio/protocol/dna-barcoding/ . If you have any questions, send us a message via the Contact Us page with details of what you'd like to learn and I'll see if I can point you towards some useful resources.
My first thought when I saw the image of the last plant was that it was Dracaena Sanderiana aka "Lucky Bamboo". So I think your matK result came close, but may not have determined the exact species.
Thank you for explaining this procedure so concisely even a newbie like me can understand. But, how to choose which primer to use? Thank you.
Use a CTAB DNA extraction, then use a modern(!) ITS2 primer and you will get to species level much more reliably. Don't forget DMSO in your PCR if using ITS2.
Check the date on the rbcL+matK paper, it's outdated.
Thanks Adrian, that's awesome feedback!! What species do you barcode?
Bethan
Good day, am Kceee. Like your honesty in your report. Am working on something similar. I used rbcL and ITS to barcode 7 plants. I want to compare the results to establish which is a better barcode. Could you give me a guide on some analysis I could do.
Thanks for the feedback 😊 Have you got results from both rbcL and ITS for all 7 plants, and are the sequencing results different?
We have a guide on our website that covers the protocols Jenny uses in this video, and works for both rbcL and ITS barcoding bento.bio/protocol/dna-barcoding/
@@BentoLab Yes I have. Please could you advise me on how to analyze the interspecific and intraspecific divergence and also check for barcoding gap. From different literatures I have gone through, these methods were used to determine which barcode marker is best for a particular plant family.
Thanks ahead.😊
@@kelechiejindu5487 That goes beyond our scope of bioinformatics knowledge on our team at the moment. Sorry we can't help more - good luck with the project!
@@kelechiejindu5487 What do you mean by barcoding gap? rbcL has no barcoding gap. You are mixing up COI barcoding with plant barcoding.
hii mam
i am intrusted in learning DNA sequencing
plz can i get your contact detail if its okey with you?
Hi, you can find us at bento.bio, and you can check out our DNA barcoding pages on our website here: bento.bio/protocol/dna-barcoding/ . If you have any questions, send us a message via the Contact Us page with details of what you'd like to learn and I'll see if I can point you towards some useful resources.
When the results are so close together, than its rather useless to find the plant. Unless you have a really small sample of the plant.
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