Hi everyone, it's Jenny here! As one eagle-eyed viewer of this vlog spotted (thanks David!), I did the dipstick extraction slightly wrong. Thankfully it still worked for the PCR and (spoiler alert!) sequencing. Moral of the story: double-check when using a new protocol, especially when you're filming a vlog! For a demonstration of the dipstick DNA extraction method, please view our video here: th-cam.com/video/QBoAHH4upIM/w-d-xo.html
a tip for getting clean uncontaminated tissue is to rip the stem into two parts and cut out a sample from the inside. don't use a blade to cut open the tissue, since the blade is "draging" contamination from the outer surface along with the cut.
hy..i find your idea quite cool...but what if we cut the stem with a blade that is sterlized on the spot (with spirit) ? or how can we cut the stem without blade i couldnt understand this..thank you
Im a myco nerd! Im into wild preservation, cultivation, breeding gourmets. Nothing better to me than working with agar in front of a fan filter unit. The way mycelium grows is fascinating to me. I have been wanting to learn dna barcoding. I think i have some studying to do! Ur video was fascinating and informative
Thanks for the comment! I agree, fungal cultures and mycelia are very cool! You can check out our DNA barcoding resources here: bento.bio/protocol/dna-barcoding/ . For fungal DNA barcoding it's also worth checking out the Fungal Sequencing Facebook group - they've got lots of useful information on their Facebook page.
I would assume that the extraction buffer from them would probably just be more pure/clean without the extra chemicals and fragrance that are in commercial dish soap. But essentially should work the same.
Hi everyone, it's Jenny here! As one eagle-eyed viewer of this vlog spotted (thanks David!), I did the dipstick extraction slightly wrong. Thankfully it still worked for the PCR and (spoiler alert!) sequencing. Moral of the story: double-check when using a new protocol, especially when you're filming a vlog! For a demonstration of the dipstick DNA extraction method, please view our video here: th-cam.com/video/QBoAHH4upIM/w-d-xo.html
a tip for getting clean uncontaminated tissue is to rip the stem into two parts and cut out a sample from the inside. don't use a blade to cut open the tissue, since the blade is "draging" contamination from the outer surface along with the cut.
hy..i find your idea quite cool...but what if we cut the stem with a blade that is sterlized on the spot (with spirit) ? or how can we cut the stem without blade i couldnt understand this..thank you
@@fatimamoazam4490 just use your sanitized hands and pull it apart without touching the inside
Im a myco nerd! Im into wild preservation, cultivation, breeding gourmets. Nothing better to me than working with agar in front of a fan filter unit. The way mycelium grows is fascinating to me. I have been wanting to learn dna barcoding. I think i have some studying to do! Ur video was fascinating and informative
Thanks for the comment! I agree, fungal cultures and mycelia are very cool! You can check out our DNA barcoding resources here: bento.bio/protocol/dna-barcoding/ . For fungal DNA barcoding it's also worth checking out the Fungal Sequencing Facebook group - they've got lots of useful information on their Facebook page.
Bento Lab is too cool. Looking forward to Part II.
Thanks! We're also curious what the results will be... :)
Can't wait to get mine!!!!
what is the extraction buffer made of? is it possible to use a dish soap & NaCl solution like the one used to extract dna from strawberries?
I would assume that the extraction buffer from them would probably just be more pure/clean without the extra chemicals and fragrance that are in commercial dish soap. But essentially should work the same.
how high did it get you?
WOOOOOHOOOOOOOO