#1GOOD

👩🏻‍🔬Good Chromatography Practices decoded first time on TH-cam 👨🏻‍🔬
GCP single liner
Always use purified water or milli Q water for mobile phase preparation.
We should always use HPLC grade or chromatographic grade solvents for the preparation of mobile phase.
Always Use HPLC grade chloroform because HPLC grade chloroform does ethanol
When we are using buffer solutions then we should make sure that buffer solution is clear, homogenous and free from particulate matter.
While preparing buffer solutions proper care should be taken to select the correct reagent i.e anhydrous,monohydrous,dehydrate etc.If specification does not tells about hydrarte status then use monohydrous salt
Adjust the Ph of the buffer solutions as per respective sop within +- 0.05 unless otherwise stated in SOP.And PH of buffer solution should always check after thorough mixing.
Buffer solutions must and should be filtered through 0.45 micron filter or as mentioned in your test method.
Friends if you will not use filtered buffer solution it will deposit your HPLC system tubings and you will phase lots of problem with respect to high pressure ghost peak etc.
Buffer solutions should not be stored for longer duration they should always be prepared freshly. If you will store buffer solutions for longer duration then there will be bacteria and fungus growth
Mobile phase should be degassed before usage but make sure that mobile phase is not kept under suction for longer duration otherwise highly volatile solvents will evaporate.
Friends we should always use glass bottle for mobile phase preparation. We should always cover mobile phase container with suitable lid to avoid evaporation of the solvents.
Mobile phase bottle shall be labelled legibly with details like date of preparation,valid up to date and compostion
Do not expose any of your solvent bottle to sunlight.
Always keep mobile phase bottle above solvent delivery system.
Always use suction filters at the beginning of mobile phase tubing’s. Suction filters will prevent entry of particulate matter into your HPLC system.
And make sure cleaning of the suction filters regularly by sonicating them with IPA and 1N Nitric acid.
Friends during the changeover of the mobile phase on your hplc system please do not keep tubing’s directly into another mobile phase. First we should rinse the suction filters with fresh mobile phase then to be used.
Thank you
happy learning....

[@pottasiri]

Hi team Pharmapill. Good morning.
Again ur team has done an excellent job. Most valuable video.
Once again tanx for new stuff. Waiting for further videos of GCP.

[@sureshkasakani289]

Please explain about analytical methods validation parameters individual and Method development briefly...

[@nilanjandey3329]

Keep posting Sir :)
Its really helpful for freshers and newbies like us.

[@azhar17kkk]

Most common buffer we use in mp preparation is KH2PO4 which is dihydrogen..so why u said to use monohydrate pls explain...?

[@PharmaPill]

see again I told about hydrate i.e water not hydrogen

[@king1234535]

Thanks for sharing

[@sknagulmeera9633]

Thank you sir, providing valuble information and good way to us 👍👍👍👍

[@sureshkasakani289]

Sir explain about CAPA, change control, deviation, plan change.

[@bhaleraoganesh5188]

Thanks for giving such a valuable information sir. Please make more video.

[@sureshkasakani289]

Sir Ur expectations are very good..
Thank you sir giving good valuable videos, thanks also ur team

[@PharmaPill]

thanks and Keep watching

[@srimahacollections.7156]

🎉🎉🎉

[@ashutoshshrivastav6204]

Thanks sir

[@deepakulai3729]

Can you tell how to integrate peaks

[@PharmaPill]

Deepa Kulai integration can be done either mannual or Automatically with the help of CDS software. Most widely used CDS softwares are Empowers & Chromaleon.

[@deepakulai3729]

@@PharmaPill can you make one video with example please

[@PharmaPill]

Deepa Kulai definitely in future i will be loading video on this topic.

[@captainpharma1315]

yes sir plz plz make video on CDS