Awesome clarity in your video and the drawings were extremely helpful. However, I'm not sure the transesterification was explained properly (in detail anyway). They are actually nucleophilic attacks by the hydroxy group of: first, the 2' hydroxy of the branch point A on the 3' phosphate of exon 1's G, and second, the 3' hydroxy of the 3' G of Exon 1 attacking the phosphate on the 5' end of Exon 2
In vitro studies have shown that most of the major spliceosomal assembly steps, including the catalytic splicing events, are reversible. This implying that there might be proof reading steps. Of course this is an in vitro study and futher research has to be carried out. But meaning its reversible means that its delta g is about 0.
Spliceosomes assemble on the transcript, as shown; it's an interesting question. As far as I know the SNRNPs are not specific to particular transcripts.
This 1D representation simply does not do it justice, it's misleading to students. Great job explaining it though for sure, not knocking the explanation.
2D, I hope, but yeah, it's limiting. And furthermore, this is getting pretty old and no doubt needs to be revised based on research since I did it. But someone else will have to.
@@agathman oh sorry, normally when I think of a sequence represented as a string I think of it as 1D; 1D was just a reference to not having RNA structure in many, if not all foundational biology texts for college students. It's an amazing explanation, thank you very much for providing it. My perspective here is trying to explain to folks how the new sequence-selective splice modulation small molecules that have recently been improved. After going into structural biology in the context of drug discovery against rare moulds, I realised that my undergraduate education lacked a foundation in structural biology. Thanks so much for the response!!
@@johnraviella6561 Oh, I see what you mean now. Yeah, there's a lot I don't know about the structures underlying this process, and certainly even more I didn't know back when I made the video. It's a very rudimentary intro to the process. Sounds like cool stuff you're doing!
6 hours of my lectures covered in 6 minutes.. you are awesome man (y) I wish we had teachers like you
AHHH! God bless you! My teacher turned this into a whopping 30 minutes of complete confusion trying to explain this.
what happens to the 2x U2AG when the B complex is formed. Do they sway with the u4,5,6 SNRP
Thank you for the explanation! very easy to understand...I wasn't that clear reading the book prior to watching this but now it makes sense!
Which protein is responsible for ligating two exons together after the intron has been cleaved?
studying for my written quals, and this was fantastic. thanks.
Awesome clarity in your video and the drawings were extremely helpful. However, I'm not sure the transesterification was explained properly (in detail anyway). They are actually nucleophilic attacks by the hydroxy group of: first, the 2' hydroxy of the branch point A on the 3' phosphate of exon 1's G, and second, the 3' hydroxy of the 3' G of Exon 1 attacking the phosphate on the 5' end of Exon 2
Awesome explanation. It makes complete sense now.
thanks for doing this, really appreciate your enthusiasm
Thank you for the video. You are a great teacher.
@RaGeLaD - Yeah, sorry about the links. Can't seem to make them work.
Shouldn't the 🔼G be negetive
cause when its Zero we gotta have equilibrium and the reverse will happen too
Degradation of the removed intron afterward probably prevents the reaction from being reversed. But I can't say for sure.
+Allen Gathman so 🔼G might be negetive or zero with degradation of products
In vitro studies have shown that most of the major spliceosomal assembly steps, including the catalytic splicing events, are reversible. This implying that there might be proof reading steps.
Of course this is an in vitro study and futher research has to be carried out. But meaning its reversible means that its delta g is about 0.
Thank you for explaining the process so well.
Are the introns spliced by the same spliceosome or do each genes have their own unique/different spliceosomes?
Spliceosomes assemble on the transcript, as shown; it's an interesting question. As far as I know the SNRNPs are not specific to particular transcripts.
Isn't it a 2'-->5' bond, not 5'-->2'? Or does that not even matter?
It doesn't matter; it's a 2' bonded to a 5'. There's no directionality to the bond itself.
Excellent explanation so easy to understand, I thank you
This was a really good explanation! Thanks!
Perfecta explicación, muy claro!
Finally. Thank you good sir!
doing gods work sir, thank you
i think this is to university level?
Very good explanation.
Thank you soo much you teach REALLY COMPLETE
bless this man
11/10 amazing video
Awesome! This was really helpful.
Incredible! Thank you sir!
great lesson
Wonderful. Thank you.
Thank you, awesome video
Thank you made things very clear
Thank you! This is explained so well. :)
great job man.
Very helpful! Thanks!
Thank you sir !!!!!
thank you so much teacher
all i can say is... i love you ~
thanks for the video
I think you mixed up between U5 and U6!!
Amazing!
So helpful!! Thank you
Brilliant!!
you just made me understand thanks
very good explanation; thanks very much :)
Great! Thanks a lot!
Great job..thank u
Dude, you're the bees knees.
AMAZING!
Thankyou!!!
Thank you for this... :)
Heh, SNRPs. They always make me giggle.
This 1D representation simply does not do it justice, it's misleading to students. Great job explaining it though for sure, not knocking the explanation.
2D, I hope, but yeah, it's limiting. And furthermore, this is getting pretty old and no doubt needs to be revised based on research since I did it. But someone else will have to.
@@agathman oh sorry, normally when I think of a sequence represented as a string I think of it as 1D; 1D was just a reference to not having RNA structure in many, if not all foundational biology texts for college students. It's an amazing explanation, thank you very much for providing it. My perspective here is trying to explain to folks how the new sequence-selective splice modulation small molecules that have recently been improved. After going into structural biology in the context of drug discovery against rare moulds, I realised that my undergraduate education lacked a foundation in structural biology. Thanks so much for the response!!
@@johnraviella6561 Oh, I see what you mean now. Yeah, there's a lot I don't know about the structures underlying this process, and certainly even more I didn't know back when I made the video. It's a very rudimentary intro to the process. Sounds like cool stuff you're doing!
Very good explanation.