Attenuation in the trp operon, part 2

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  • เผยแพร่เมื่อ 24 ธ.ค. 2024

ความคิดเห็น • 144

  • @agathman
    @agathman  12 ปีที่แล้ว +6

    Thank you. I think when I retire I'll make more of these just for fun.

  • @imaginepeace2015
    @imaginepeace2015 10 ปีที่แล้ว +19

    You literally explained in 12 minutes what took my Microbiology professor a week and a half to teach. The difference is that I actually understood the concept! I know this video was made years ago, but I cannot possibly thank you enough for taking the time to make it!

    • @agathman
      @agathman  10 ปีที่แล้ว +15

      Thanks for the kind words. It's been a while now since I've been in the classroom, but it's still nice to hear that these videos have some value.

  • @tungtran6071
    @tungtran6071 3 ปีที่แล้ว

    I don't know why my nucleic acid professor did not explain the concept in this way. It makes much more sense! Thank you so much!

  • @MrPupp123
    @MrPupp123 11 ปีที่แล้ว

    You explained it better in 10 minutes than my professor did in 60. Thanks so much!

  • @BlueSkyFight
    @BlueSkyFight 11 ปีที่แล้ว

    You are such a great teacher!!!!! Thank you sir :) Im an spanish girl studying biochemistry and I have just 100% understood the attenuation system due to this video... I wish every teacher and scientist in the world got your enthusiasm and teached that good. Thank you :) and remember that "teaching means learning again" !! :):)

  • @imarevolutionary27
    @imarevolutionary27 14 ปีที่แล้ว

    Oh my god I love you. Biochem final on Tuesday and this is exactly what I needed. No one ever bothered to explain where these Trp codons were. This makes it immensely more clear. Thank you thank you

  • @Ladylyla
    @Ladylyla 12 ปีที่แล้ว

    I wish the people that teach here at my university would do it as clearly as you do, this here seems so simple and beautiful!

  • @jbramson33
    @jbramson33 13 ปีที่แล้ว

    really an excellent explanation. I am a first year medical student, and your presentation is much clearer to what we saw in lecture!
    Thanks!

  • @youngkim4937
    @youngkim4937 9 ปีที่แล้ว

    talk about a moment of epiphany!
    Crystal clear explanation.
    Thank you.

  • @thelosian
    @thelosian 10 ปีที่แล้ว +6

    THIS WAS AWESOME!
    sad that I'm paying thousands upon thousands of dollars to learn this in dental school and this is appreciably better... for free. Thanks for your time!

  • @Habib-um2oc
    @Habib-um2oc 10 ปีที่แล้ว +1

    When this video was over, i had the biggest smile on my face because of how well i understood what you taught. Keep it up Sir :)

  • @Mruk4
    @Mruk4 12 ปีที่แล้ว

    Oh my God, I wish you were working at my university!!! Your explanation is so clear and easy to follow, once again thanks a million agathman!

  • @TheRockstarGal
    @TheRockstarGal 11 ปีที่แล้ว

    THANK YOU SO MUCH!! I'm a second year biomedical and it is only because of you that i have understood the concept. I wish my teacher was half as good!! PLZ PLZ mk more of these!!!

  • @LunaticRD
    @LunaticRD 10 ปีที่แล้ว

    thank you for this video.. it all made sense in 10 min of watching 2 videos.. over 1 hour of my prof trying to explain this and failing miserably.. thank you!

  • @olofgote
    @olofgote 13 ปีที่แล้ว

    Thank you! Have a test tomorrow and the book couldnt help me figuring out how attenuation is working. This helped me.

  • @marisasmith8750
    @marisasmith8750 10 ปีที่แล้ว

    Wow, I have been struggling with this understanding for a couple weeks now and in your short two videos, I completely get the concept. The only things I think I had trouble wrapping my brain around were when you drew the DNA and RNA polymerase, but I'll go ahead and read up a bit more on it to figure it out and watch your video again. Thanks a lot for this! Really helped me out!

  • @aqfanjames
    @aqfanjames 13 ปีที่แล้ว

    You are the man. Keep up the good work! My lecturer bores me to sleep. Kudos to you.

  • @pinkfire29
    @pinkfire29 13 ปีที่แล้ว

    This helped me understand the concept so much more, I think I might pass my molecular biology exam now! Thank you :)

  • @SPDMorgan
    @SPDMorgan 11 ปีที่แล้ว

    Thanks very much, you did a much better job of explaining it than my lecturer!

  • @Mruk4
    @Mruk4 12 ปีที่แล้ว

    I simply LOVE gentics! Great explanation! Very clear and easy to follow. It's easy to believe you know the topic very well by listening to the way you explain it! To make is short - without a doubt, never have I seen such a good explanation! I used to think I know the process of attenuation. After seeing that vid I know that it's only now I don't just know it but also understand it! THANKS A MILLION! :)

  • @mardouk1992
    @mardouk1992 12 ปีที่แล้ว

    Understood perfectly every single thing! Thank you! If used right after 'Gene Regulation, by bozemanbiology' (which gives you the simple facts,trp is after the lac) it's really useful to enhance what you already know.

  • @htboardman
    @htboardman 10 ปีที่แล้ว

    Thank you so much for posting your videos, you've explained EVERYTHING so clearly and simply. This will sure as hell help me with my resit...

  • @sassy2021
    @sassy2021 12 ปีที่แล้ว

    OMG THANK YOU THANK YOU THANK YOUUUU! I have my genetics exams in 5 hours and you just saved my life! Thank you!!!

  • @randomcoz
    @randomcoz 14 ปีที่แล้ว

    I'll second edowny0606 here - missed this lecture at uni and the notes didn't make sense and this just saved my butt, exam in two days! booyaa!!
    thanks agathman!

  • @By_The_CShore
    @By_The_CShore 10 ปีที่แล้ว

    This is extremely helpful, very good explanation of how this system works.

  • @agathman
    @agathman  11 ปีที่แล้ว

    If 3+4 pair, the stem loop that they form is an intrinsic transcription terminator. The stem-loop binds to a site on RNA Polymerase and causes it to stop transcribing there, before it gets to the coding region of the operon. This is how transcription ends normally in most genes in E. coli, but at the end of the gene instead of the beginning.

  • @jonahfox
    @jonahfox 14 ปีที่แล้ว

    You explained this much better than my professor. Thanks.

  • @nsbutler1
    @nsbutler1 11 ปีที่แล้ว

    Explained better then my lecturer, thanks so much!

  • @Dancingleebo
    @Dancingleebo 8 ปีที่แล้ว

    Again, described really well. You have truly helped me understand this. Thank you.

  • @agathman
    @agathman  13 ปีที่แล้ว

    @Sbrenzen -- I believe it's read-through. That is, the ribosome moves along the RNA after the stop codon and starts translating when it reaches another start codon; this is how the ribosome translates the multiple cistrons in a typical polycistronic mRNA in a prokaryote.

  • @agathman
    @agathman  13 ปีที่แล้ว

    @Sbrenzen No, the stem loop (if it's the 3/4 stem-loop) can terminate transcription, but translation doesn't seem to be affected much by secondary structure of the mRNA.

  • @ddsschool7102
    @ddsschool7102 12 ปีที่แล้ว

    Thank you so much!!!! This makes it so much easier than our biochem teacher makes it!

  • @agathman
    @agathman  12 ปีที่แล้ว

    No, transcription doesn't automatically terminate whenever there are several Us. The stem-loop is important in the termination process. For one source with more info, there's an article at PubMed Central called "The functional anatomy of an intrinsic transcription terminator", by Schwartz et al. that gives some background (and more refs) in the introduction.

  • @FranciscoAlmeida-pq6ox
    @FranciscoAlmeida-pq6ox 8 ปีที่แล้ว

    Thank you for explaining this process and clarifying my doubts about it! It was really helpful, keep up the good work, cheers!!

  • @atulpradhanjerry
    @atulpradhanjerry 8 ปีที่แล้ว

    The best concept I got about attenuation. Thanks Allen Gatman

  • @silversky14FM
    @silversky14FM 11 ปีที่แล้ว

    wow love it .. thank u very much

  • @anonymousresearcher6581
    @anonymousresearcher6581 12 ปีที่แล้ว

    thnx sir....this video just cleared a lot of my doubts....i got so confused reading abt this in d books...bt ur explanation ws perfect....thnx a lot.

  • @agathman
    @agathman  12 ปีที่แล้ว

    Yes, the ribosome is translating the leader region. And transcription is simultaneous; the ribosome is translating the beginning of the mRNA -- the leader -- while the rest is starting transcription. If regions 3 and 4 of the leader can pair, they form a transcription terminator. Transcription then terminates, and the rest of the mRNA (the part that codes for the enzymes involved in tryptophan synthesis) doesn't get transcribed.

  • @MsHistoryprojects
    @MsHistoryprojects 9 ปีที่แล้ว

    This just cleared up every question I had about this. Thank you so much for this! You're going to be the reason I ace this upcoming quiz!

  • @monomnn
    @monomnn 13 ปีที่แล้ว

    Thank you so much, I read this section and I was so confused this regulation and you finally clarify this. :D

  • @Nat-og5jd
    @Nat-og5jd 9 ปีที่แล้ว

    Such a nice video...you explained it so well in such a short time....thanks a lot.!

  • @Xeph-yrs
    @Xeph-yrs 13 ปีที่แล้ว

    You are a great professor. I hope you have tenure if you have it already!

  • @flatlandmountaineer
    @flatlandmountaineer 13 ปีที่แล้ว

    Thank you for sharing! from a medical student at a top 20 school (and you beat my prof).

  • @supadupafly16
    @supadupafly16 12 ปีที่แล้ว

    Thank you soo much for this video! I got so confused when reading about this in books

  • @bigudukaz
    @bigudukaz 14 ปีที่แล้ว

    tomorrow is my exam, and thats the part i didnt understand :D nice work - saved me :D

  • @Smilemon5953
    @Smilemon5953 12 ปีที่แล้ว

    Very helpful video. I have a Microbiology final in an hour and this cleared some stuff right up. You're drawings are easy to understand. You should get a tablet and make some flash videos.

  • @IntelligencePrevail
    @IntelligencePrevail 12 ปีที่แล้ว

    Thank you so much, please do make more, It was very helpful, I have got exam on Genes and Genomics in a few days time =)

  • @marocaaan
    @marocaaan 14 ปีที่แล้ว

    Thank you very much for your explanation, i finally get it. Greetings from The Netherlands.

  • @dhawanrawat
    @dhawanrawat 13 ปีที่แล้ว

    Brilliant.. great style of explaining!!!

  • @agathman
    @agathman  11 ปีที่แล้ว

    RNA polymerase binds to the DNA and synthesizes RNA complementary to it. The ribosome binds to the RNA and translates it. They're binding to different molecules. If the RNA polymerase hasn't reached region 2 (in the DNA), then it hasn't made that part of the RNA yet. So the ribosome couldn't possibly get there first.

  • @chrisyeahismyname
    @chrisyeahismyname 11 ปีที่แล้ว

    This was a fantastic help. Thank you for the clear explanation.

  • @Nikvaylo
    @Nikvaylo 14 ปีที่แล้ว

    Thank you for this video. It explains basics of attenuation perfectly! :)
    Thanks...

  • @Sbrenzen
    @Sbrenzen 13 ปีที่แล้ว

    @agathman Ok, I got it. I believed that ribosome blocked once reached the stem-loop. Thank you so much (and sorry for english mistakes) !!

  • @hollandaiserox
    @hollandaiserox 14 ปีที่แล้ว

    Thank you very much! I found this confusing but you explained it perfectly!

  • @poojagaikwad8660
    @poojagaikwad8660 11 ปีที่แล้ว

    thnk u sir it made me clear all my doubts abut attenuation in trp.operon..

  • @justwerent
    @justwerent 13 ปีที่แล้ว

    This was extremely helpful, thank you so much!

  • @poojagaikwad8660
    @poojagaikwad8660 11 ปีที่แล้ว

    thnk u very much sir it made me clear all doubts about attenuation...

  • @MA-kd8rl
    @MA-kd8rl 9 ปีที่แล้ว +2

    How can the ribosome pass through the stop codon of the leader sequence and continue moving along the mRNA to the start codon of the TrpE gene?

  • @samirahassan6986
    @samirahassan6986 12 ปีที่แล้ว

    Thank you, I wish my lecturer explained like this .

  • @padmagn5697
    @padmagn5697 8 ปีที่แล้ว

    This video was really very helpful ...thank u so much 😀😀😀😀😀😀😀😀😀

  • @RusW0lverine
    @RusW0lverine 12 ปีที่แล้ว

    thank you sir, for your clear and informative explanation, i hope you will do more videos about genetics.

  • @dr.krishnakumarj1670
    @dr.krishnakumarj1670 8 ปีที่แล้ว

    i am professor of biotechnology. its very useful for me.

  • @Chaunguyen1502
    @Chaunguyen1502 11 ปีที่แล้ว

    you saved my life. Thank you so much

  • @Sbrenzen
    @Sbrenzen 13 ปีที่แล้ว

    Many thanks!
    But I have a question: when transcription continues, can the ribosome start translation directly from the AUG codon at the beginning of the trpE? Is there a ribosome binding-site?

  • @araino262
    @araino262 11 ปีที่แล้ว

    thank you so much for making this video!

  • @lolowhat02
    @lolowhat02 10 ปีที่แล้ว

    Thanks, much clearer than my med school prof!

  • @TuyenNguyen-vg8sl
    @TuyenNguyen-vg8sl 10 ปีที่แล้ว

    Thank you very much for the video. I have a question, IF 2 AND 3 make a stem loop, how can ribosome continue to translate the sequence?

    • @hammeredgrannies7821
      @hammeredgrannies7821 10 ปีที่แล้ว

      Translation isn't impeded much by secondary structure in the RNA. I suppose that the ribosome must be able to break hydrogen bonds between RNA strands as it passes through, but I'm speculating.

  • @bradberan
    @bradberan 13 ปีที่แล้ว

    @yorevolution He is my professor and my advisor. He definately knows what he is talking about.

  • @vernonasian
    @vernonasian 13 ปีที่แล้ว

    Thank you so much, many many many thanks for your time!

  • @Sbrenzen
    @Sbrenzen 13 ปีที่แล้ว

    @agathman Great! Thank you for your prompt reply! Now I wish my examinator ask me about attenuation!

  • @whitsluss1
    @whitsluss1 10 ปีที่แล้ว

    Thank you so much, made it very simple.

  • @Jonesle90
    @Jonesle90 12 ปีที่แล้ว

    I'm so confused by this one little part - in attenuation when the ribosome is going through the leader sequence, you say if [trp] is high, the ribosome moves quickly through region 1 and when it's in region, it 2 blocks region 3 from pairing with it so region 3 pairs with region 4. What is the ribosome doing? Translation? Are the structural genes only transcribed if the leader sequence is transcribed and translated correctly? I thought in prokaryotes that the two processes could be simultaneous.

  • @fnixmiya1180
    @fnixmiya1180 10 ปีที่แล้ว

    Thank You this vid was a GREAT help for me

  • @CommanderCody93
    @CommanderCody93 8 ปีที่แล้ว

    Thank you very much, really good explanation :)

  • @CarolDCruz
    @CarolDCruz 11 ปีที่แล้ว

    Sir, Query related to attenuation: how does d transcription end when the [Trp] is high i.e. as u said first it moves away quickly from region 1 of mRNA as theres plenty of trp in surrounding, then region3 is transcribed followed by region4's transcription & as they are palindromes & region 2 is not free region 3&4 form a stem loop....... Further i havnt understood from the point how the trancription has ended at 3-4 stem loop. Please sir explain me just this part in text format. Thanks.

  • @emilysathin3958
    @emilysathin3958 10 ปีที่แล้ว

    Thanks alot!!! really good explain, great job

  • @sohinideb2095
    @sohinideb2095 9 ปีที่แล้ว

    That helped. Thank you soooooo much. Thanks alot. You explained it so nicely. Thanks again :) :)

  • @jahnusaikia1901
    @jahnusaikia1901 10 ปีที่แล้ว

    good work allen....... keep it up

  • @The0Vivacious
    @The0Vivacious 11 ปีที่แล้ว

    That was AWESOME!!! Thank you, you rock!

  • @Jonesle90
    @Jonesle90 12 ปีที่แล้ว

    Thank you! That makes better sense to me, now.

  • @martinacorte12
    @martinacorte12 12 ปีที่แล้ว

    Excellent video! Thank you very much :)

  • @Giulia-lo7gt
    @Giulia-lo7gt 10 ปีที่แล้ว

    you really helped me! thank you so much! good explaination

  • @sujsuku
    @sujsuku 12 ปีที่แล้ว

    I have a question.......when trp levels are low, even though there is no loop formation between 3 and 4 regions, doesn't the presence of U repeats cause transcription termination??

  • @AhmedK81
    @AhmedK81 12 ปีที่แล้ว

    Wonderful !! nice explanation

  • @Mojoelmo
    @Mojoelmo 14 ปีที่แล้ว

    Thankyou very much, explained very well :)

  • @indian1534
    @indian1534 4 ปีที่แล้ว

    Thanks ,clearly understood!

  • @RK-kj1ze
    @RK-kj1ze 9 ปีที่แล้ว

    Ah Thank you! I finally get it now.

  • @Ak7kid
    @Ak7kid 13 ปีที่แล้ว

    amazing! REALLLY helped!

  • @hamadaliberton7398
    @hamadaliberton7398 9 ปีที่แล้ว

    thank you.. it is even fun to study such a mechanism

  • @andrew1007
    @andrew1007 14 ปีที่แล้ว

    fantastic explanation

  • @agathman
    @agathman  13 ปีที่แล้ว

    @sDlF309 -- Sorry, I should really put the logo on my vids or something. I'm at Southeast Missouri State University in Cape Girardeau, MO.

  • @bigblueltz71
    @bigblueltz71 14 ปีที่แล้ว

    Thank you very much!!! very helpful!

  • @Troklo
    @Troklo 14 ปีที่แล้ว

    you sir are brilliant! Thanyou so much!

  • @TheEbi78
    @TheEbi78 10 ปีที่แล้ว

    Superb Explanation

  • @ltripp1991
    @ltripp1991 13 ปีที่แล้ว

    great video, so clear! :)

  • @wanes8958
    @wanes8958 9 ปีที่แล้ว

    finally i understood .... thank you so much

  • @LKP0904
    @LKP0904 12 ปีที่แล้ว

    Yeah, finally i can understand, thanks!! :)

  • @vellyjatt92
    @vellyjatt92 14 ปีที่แล้ว

    this is so helpful....thanks..

  • @MrPridizzle
    @MrPridizzle 10 ปีที่แล้ว +1

    Thanks Allen
    --Royce

  • @rubster09
    @rubster09 12 ปีที่แล้ว

    Excellent! Thank you so much! :D