Quantitative ELISA

For more information, visit www.bio-rad.com/yt/2/lse-ELISA.
This video demonstrates a quantitative ELISA (enzyme-linked immunosorbent assay) using the Bio-Rad ELISA Immuno Explorer™ Kit. Serial dilutions of standards are performed and used for quantitation of test samples. Assay steps, including addition of samples to the microtiter plate strip and washing steps are shown.
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[@ijhkhk]

Thank you sir for making ...such practical demonstration...I am a biotechnology student ....and its more helpful for me and also for various biotechnology, immunology, mol biology and biochemistry student......please make such wonderful videos for us....you are doing fantastic job thank u very much

[@BakariAziz]

Question: what is the purpose of the six "control samples" in the second well if we're using the first well as our standard/control?

[@abrorbeksharifjonov6365]

Why there are not used block step and incubation during all processes are too short, usually all steps take an hour?

[@TanTan-ch3vq]

Where is the blocking step?

[@123fronte]

wow, you eye balling your ELISA instead of using a plate reader?

[@NoName-dr5zc]

Great demonstration!!
Why do I cringe when I see your pipette tip touch the samples and then put back into the tubes though?? 😅😂
Does that mean that it’s not too sensitive?

[@curious.soul.]

The aliquots don't serve any DS use or may be its only for demo purposes.

[@LuzRengifo]

It is in english. I don´t understand😥

[@tagorecbse2096]

if you don't know English ,go and learn in tagore 🤣🤣😂😂