HIC Chromatography

For more information, visit www.bio-rad.com/yt/gfp.
This video demonstrates the technique for protein purification using the Bio-Rad Green Fluorescent Protein (GFP) chromatography kit. Recombinant GFP is purified from a bacterial lysate using hydrophobic interaction chromatography (HIC).
www.bio-rad.com/evportal/desti...
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[@sh7arwt3teer99]

please if you can explain to me, why the mobile phases containing a concentration of ammonium sulfate promote the binding of many proteins to HIC columns??

[@TrevonCoppin-ci1ig]

great vid !!!!!!!!

[@julesteuwen]

What resin is actually used?

[@julesteuwen]

+BioRadLifeScience Thank you!

[@cutepapaya4886]

GrE eN fl UoR eScE nT Pr Ote iN