Isolation of Plant Pigments by Column Chromatography - Amrita University

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Column chromatography devised on the basis of differential adsorbance(partition coefficient) of substances on solid adsorbent (eg:silica or alumina) to an extent, that depends on the substance polarity (considered for solubility) and other chemical properties and structural properties. Some compounds are adsorbed more strongly to the stationary phase than other and thus they will be eluted (washed down) the column at a very slow rate, thus can be separated on the basis of their elution rate accordingly. Thus naturally coloured components of spinach Spinacia oleracea) and other leaves can be separated by this technique. The dark colour of the spinach is a combination of β-carotene and chlorophylls.

[@budioctasarisusanto1267]

Wow, thank you so much for the video. It is really helpful. I've been looking for mobile phase to separate chlorophyll

[@md.tauhidulislam8008]

It's really helpful. But I have a question that "Do you use any filter paper for filtering in different stages?". In the video I have not seen any filter paper. How do I filter the solution?

[@asif119khan]

Thank you.

[@romfeastore]

very good

[@devigag6647]

it's very useful

[@macracwhite]

Little Fast but thank you for making it.

[@hemaahmed1937]

good