Mitotic Index Root Tip Squash

Observing Mitosis in Root Tips - A level biology required practical activity. Make sure you know how to calculate mitotic index. The notes towards the end of the clip explain this.

[@albankapaj859]

You have saved me and my class from failing the practical

[@yus_hijazi1858]

Its true, i am the teacher

[@harveyl1032]

Sorry sir

[@user-wv6gf8js7t]

@@yus_hijazi1858 XD

[@ibbypatel172]

No 1 cares

[@user-gv4un3bd4s]

@@yus_hijazi1858 દત્ત એકશષ

[@vathsalavathsaladev830]

Really AMAZING ...... It was very much useful to me ..... THANKZ A LOT 😊

[@sirfmgamer5655]

Well done for taking time out to do this :)

[@jamesragsdill1034]

Insane how good this is.

[@astrosung]

could you please include an equipment list as that would be very helpful. thanks

[@kairiannah]

these are great for private students cheers!

[@lynnelizabethcoelho6116]

Very useful 👍🏼

[@zachreyhelmberger894]

Thanks for the presentation!! Would LOVE to see the slide you just made under the scope!!

[@mahmoudmoez3807]

thank you a lot that was very helpful :D

[@raisajahan5839]

it is very helpful thankyou very much

[@AvaneeshYadavkingoftheworld]

You're amazing!

[@soniasabu2053]

oh stop it u

[@arwa8388]

very beautiful . thanks

[@soniasabu2053]

ur welcome love

[@birdie7344]

thank you

[@susmitamondal4795]

Thank you

[@blackdogwoof]

Big legend

[@aboowe4801]

Thanks

[@avinkushi]

How to prepare 2 molar hcl??

[@hansiniimehta7007]

Thanks❤️

[@kannansathya6245]

Super

[@tamerzaghlol9461]

Thx

[@Lily-cl6zk]

queen

[@soniasabu2053]

king

[@luca_magna7753]

prince

[@huh1327]

princess

[@urwahxx3399]

Hi I'm doing this practical this Wednesday, would you be able to provide a link that has a written method and equipment list please? Thank you!

[@o.m9514]

The hot plate looks....., is it heating up by convention heat transfer?

[@o.m9514]

Cus it doesn't look like conduction to me...

[@marshalkent4735]

what do i put for my hazard assessment

[@biologypracticalsandrevisi8066]

marshal kent concentrated acid with fumes when you heat it. Sharp blade to remove the tips.

[@zenmanproject]

Is the acetic acid stain necessary or will other stains work? I'm struggling to find it. If the acetic acid is part of the process, is it stronger than 5%? That's the strength of off the shelf vinegar.

[@biologypracticalsandrevisi8066]

You can use a stain called Toluidine blue but you do need a strong acid. Usually 5 molar HCl. You will only be able to get these from a scientific suppliers. I have to warn you that it is difficult to get good slides showing all stages of the cell cycle. So I wouldn’t bother with this if not absolutely necessary. You can buy ready prepared slides and there are good images on the internet.

[@zenmanproject]

Seeing images on the internet or slides prepared by someone else is not even remotely the same experience! We'll keep trying.
I can make 5 molar HCl. That's easy enough. This afternoon we tried using Methylene blue and Carbol fuchsin with 1 molar HCl (that's all I had on hand). We didn't have much luck. I'll try some stronger HCl next time.
What is the HCl doing to the cells? Is it breaking apart the layers?

[@biologypracticalsandrevisi8066]

Really any strong acid should do and as I said try and get some toluidine blue stain. Hope it works out for you. It is a nice activity.

[@zenmanproject]

@@biologypracticalsandrevisi8066 Thank you. We will experiment more. I'll try to get some toluidine blue. This is a really fascinating project. Now I'm excited to try out some of your other projects next. :-)

[@induanamika1778]

Hello Ma'am , actually i have one problem during squashing the root tips. the stain is moving out of the cells and i can't see any mitotic phase. please give me some suggestion.

[@betul-ev2fu]

note: vertical force

[@banimaisnam4806]

Hello Edmundian😁😁😁

[@venkateshbabu5623]

Simple phase changes visual and process.

[@soniasabu2053]

amen sista

[@gemmahemens1618]

Why is it heated?

[@soniasabu2053]

so that the excess stain evaporates away while still keeping the stain on the root top

[@gazarkhalid840]

In the exam ,you can just write that heating intensifies the stain colour

[@urmomsah0e476]

I love u

[@maryam9056]

The mitosis meiosis chapter is delusional idk made me loose interest in bio

[@Shopping_centre786]

On 2:55 what u added

[@_-_-__-_-_5428]

Acetic Orecin stain

[@anishasrikar4996]

Why do you add the Hydrochloric acid?

[@biologypracticalsandrevisi8066]

HCL quickly denatures any proteins including enzymes. It quickly stops cellular processes and kills cells halting the process of mitosis immediately so that cells in different stages of the cell cycle can be seen.

[@anishasrikar4996]

Thank you for the detailed reply! Extremely helpful. I have another question. I've seen people adding ethanoic acid on the slide before they place the cut root tips on it. Is this for cleaning the slide or does it serve a completely different purpose? Thanks again! This was a very useful video and it's helping me for my IB Bio IA.

[@sammadsaeed3373]

Acetic acid has very low pH, which weakens hydrogen and ionic bonds.
In young cell walls, pectins are largely acidic and then prone to establish electrostatic interactions with cellulose and hemicuellulose. In older tissues pectins are methyl-esterified -- more hydrophobic -- and harden at acid pH.
Chromatin behaves roughly the same way. Interactions between histones and DNA are mostly electrostatic, so weaken by pH lowering.
Weakened interactions between histones and DNA result in softer chromosomes that can be easily squashed
Hope I am not that late lol

[@courtneychambers319]

Anybody with the open study college? Studying A-level biology from home?

[@elettrabelaqua4575]

I'm not with open study but yeah I'm studying from home...oof

[@addiepat6552]

what is the staining chemical ypu add

[@mariamabed753]

addie pat acetic orcein stain

[@biologypracticalsandrevisi8066]

Acetic Orcein stain or Toluidene blue stain.

[@user-gi3yr1gl7j]

Increase contrast to make the chromosomes visible

[@singlefather9796]

Gay

[@dr.bouacham.1895]

thank you