Spore Syringe to Agar - The Agar Files
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- เผยแพร่เมื่อ 20 ก.ย. 2024
- Today's class I'll be going over 3 different methods to germinate spores from a syringe onto agar. Thank you as always for the love and support.
Inoculation Loop Video - • Cheap and Easy DIY Ino...
0:10 Intro and Prep
2:17 Shake up your Spore Syringe
4:55 1-2 Drops on Agar
5:23 Inoculation Loop on Agar
6:28 Sterile Swab on Agar
7:28 Cleanup and Storing your Agar
8:30 7 Days after inoculating
9:20 10 Days after inoculating
10:16 Outro
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As a medic I've found that if you pull back on the syringe before you push the solution out you have much more control over how much comes out during that first push.
But you risk letting contams enter the syringe probably
Adding on to my previous reply, if done in a still air box it would probably be kinda safe
You can place a just slightly damp alcohol wipe over the end first.
As an addict in recovery I can confirm that it's much easier if you pull back first
Risking pulling in contaminates in the air? Why risk it
I purge that first push into a shot glass, its all dead spores anyway you just cooked um.
here is another tip:
with the syringe cap on tight pull the plunger a short distance and quickly let it go, it will create a shockwave & disperse the spore clumps.
Nice, thanks
Here's a tip: throw soil on top of your sub when your done fruiting it to make a indoor compost bin. Lots of fun
"Break this JAWN Apart" Philly is Really in the house.
Another tip for controlling drops is just squeezing the sides rather than pushing the plunger. Thanks for what you do for us PGT!
That awesome, you just use it as a pipette. Genius.
Syringes are hard plastic.
That can introduce air back into the syringe
@@fungdark8270 you do it without the syringe
Yeah Bro..
You are a Genius..
Newbie here and your content is great!! I’m learning sooo much. Thank you for explaining everything extensively. After watching other channels and finally hearing you explain things to where I understand it - you hear many “ohhhhhhhs” coming out of me. Your channel is quickly a favorite. Thanks for all the great content!
3 videos in one weekend! You the man 🤙🏼, we appreciate all your efforts. I've been growing cannabis for 10 years and now with the help from you, and members of the mycology community on TH-cam and reddit, I've been slowly buying all my supplies over the past few weeks while I study the craft before I start. This will probably never get read but thanks brother ✌🏼🍄
EDIT: 8 months later and I’ve grown pounds of TATs 😂🤙🏽 (side note) I also just had my very first contamination 🤙🏽
Dude just go for it! Learn on the job :)
Best. Hobby. Ever. 🤙💚
I could say the exact same thing as Psychonaut. Been growing herb for years and thanks to Teacher and a few other, my fruiting room is ready and I'm waiting for my spores to come in. Can't wait.
F TH-cam and Reddit. If you want info on cultivating, foraging, or anything that has to do with mycology. Then go to The Shroomery.
@@michaelcoleman5922 Where did you get your spores?
Sensei, thank you, thank you, thank you! I'm starting my journey in mycology and microdosing to help my grandson who suffers from epilepsy, Crohn's, and depression. Of all the DIY videos I've watched, yours call to me. I've inoculated agar plates and made brown rice mini-jars (pint). Wish me well!
You sound like an amazing grandmother! Hope all is going well :)
haha nuts to see an older woman make drugs
Much love to you and your grandson. Hope you're both doing well.
your such an amazing woman and grandmother. I hope your seeing amazing results from the plant medicine to help your grandson. I truly believe mother nature gave us everything we need. Blessings to you and your grandson.
Hey, I’ve recently had my first two flushes ever and want to thank you man, I’ve got knowledge from you that I’ll hold forever. Thanks alot
The confidence wrapping with the parafilm shows pure practice. ❤
Hello PGT, really just wanted to express my gratitude for all your hard work in the videos that you're doing, I'm a newbie at this only about 3 weeks into it but I'm so excited about it, I don't know why I'm so drawn to it, I think you're doing such a wonderful job and I really just wanted to convey that, thank you so much.
@ 5:08 you mention the first depression of the syringe causes way too much to come out. I was hoping you were gonna say something. I work in the medical field and our saline flushes do the same thing. To resolve this, simply pull back on the syringe, it breaks the stopper free and then smoothly moves forward. ;-) But be careful not to pull it backwards out of the body, just a tiny bit to free it up, twisting sometimes helps too.
Man, I wish this was posted yesterday at this time! I just did the squeeze one drop method. Should have done the streak method. Oh well, thanks PGT!
same here
I have way more than a couple drops
Having a shitty day... came home took some food open youtube and new video from PTG! Now my day is a lot better :)
Love learning from someone so knowledgeable, and I absolutely love your video style. You put a lot of work into this channel, good freaking job my man
Idk if he ever says so in the video, but definitely store your agar dishes/cups upside down before and after inoculating to cut down on contam caused by condensation mushlove
i remember learning this in my freshman year of highschool in biology 😂
@@Yokoname that's way cool! I unfortunately had to find this out the hard way I lost all my first agar cups to contam bc they weren't upside down
Most amazing coincidence this month! Love your videos and literally this weekend got plates to do SS to agar, glad I waited you read my mind!
Wow! Vegan ham candy! This video makes me so happy! Thank you!
I'm glad you are feeling better 💜
So good. You make info that seems incomprehensible simple. I’m stoked to start working with agar. 👍
I'm glad I could help. Good luck starting your journey with agar! 😊
I'm just a beginner and this video was extremely helpful. Thank you.
Thanks for the content! Not trying to sound unappreciative with my last comment - it's just helpful to get an idea of the process/order of events when working with an SAB because it can confusing trying to parse together when to go out of the box then back in to flame sterilize the needle etc without disrupting the still air.
Been watching lots of different videos to try to piece it together, this one helps a lot!
Please make a video about how you made your still air box. I'm getting into the hobby (collecting my tools and supplies now) but have yet to see a good how-to on a good still air box and yours seems luxurious as you have room for a camera inside. ;)
Many Thank yous dear Jack! Looking forward to seeing where the peitre dish goes next!! 😉
😄
I can see some white stuff in one of my bags. What now?? Thanks you!
@@karinchristensen9809 Well?
What Happened Next?
I suggest leaving out the shot glass and picking up a 5ml graduated cylinder. It helps me to avoid using too much solution. 1ml will innoc dozens of plates. Also, a pipette gives much more control over the single drop. [5ml grad cylinder w/ glass pipette $6.99 @ Amazon] Same equipment can be used to transfer LC to plates or slants.
I like the sound of this method. How much liquid do you drop onto each plate?
@@ChrisMosner
Literally 1 drop or less is all that will be needed.
your whole channel is a blessing!
Always nice to see a good education video from u mate!
Thanks a ton PGT. Thanks in a big part to you, I just received my 1st mycology research kits today. Your lessons have been invaluable in my learning and planning . Now I just need to pour those 200 agar plates. :) Thank you.
literally what I was looking for, just ordered spore syringes yesterday and was wondering if I could start messing with agar, thanks!!!
Great vid , I was wondering how to put spores to agar 💯💪🏾 you answered all my questions
Well darn. I could have used this video yesterday. Searched a bit and this pops up today. I used the direct inject method onto the agar. Shaking up all the spores was the hard part. Didn't think to add a slight touch of air to help the solution shake better. Figure the cotton swab method will work best.
Only had one successful grow 2 summers back. This summer i had bought all this stuff, going for some oats jars and pf tek jars... I used agar and thought I had some good plates, they looked good. I cut up the agar, plop it into the jars, they colonized quick. Then i opened them to mix w coco coir and peeee yew smelled rancid... Slightly yellowish. Had to dump it. Gonna go at it again. I pressure cook everything lol! I don't have a good, use a SAB.. something got in it. Well spring is here, time to try again
Love your videos bro! Just wanted to add my own cheap and easy inoculation loop I use. I just got a black head popper from the dollar store 😂😂😂, all stainless steel and can be put in a PC to sterilize if necessary
Yet another great video Teacher! You show so many great ways to get shrooms started that it's nearly impossible to fail! Thanks again!
Thanks for this, I prefer using Agar cups, but once I'm done growing for a season, I put everything away. All I have left is spore prints in storage. Going from spores to agar allows me to skip a step.
Thank you bro, just getting started with some lions mane, King Oyster, and others, I picked up some innoc loops and sterile swabs for a few bucks on Amazon, will be swabbing in a couple of days, thanks for all your great vids. After agar grows out, will transfer to agar, and l.c. and see how things progress, then onto my masters mix, and grain/ verm mixes to see which varieties grow best on each medium...thanks again.Pgt
Homeade ingenuity brother, been following your ways for a minute with great luck forsure (: lol keep up the good work!
Wow when you were tapping the spore syringe on your finger it exactly matched the music in the video
😄
I knew I forgot something . Well I'm learning . Thank you for sharing your knowledge . It's literally gold ✨️ .
No matter what I do, I get massive condensation in my agar plates. Any tips? Thanks pgt & glad to have you back.
You can stack your plates right after pouring, the heat will rise through the plates and remove condensation from the lower plates. the best method to eliminate condensation completely is to leave your plates in front of the flowhood for 2-3 days after pouring. the laminar flow slowly makes its way through the plates and dries up condensation.
I gave mine a quick shake, and was able to get droplets to condense, to see spires growing, but now I gotta leave it tilted or upside down till the water dries up.
How can you tell the difference between mycelium and mold? They look so similar except the mold wasn't as thick looking
You make the best videos on your tube .. thanks again bro
Mush love been following you since high school love your work thanks so much for your inspiration!
Great videos!
I need to get better at spotting contamination. I thought that plate was good. I'm gonna check more videos , you probably have one about spotting contamination already.
Hi my name is Lance. I am a new grower and I just watched one of your videos and I like how simple your videos are been watching a lot of other videos trying to learn the mycology. Just wanted to say thanks and I’m going to watch more of your videos
Thank you lance, I wish you the best on your journey! 😁🍄
I've been using zip lock sandwich bags to store my plates in, after micropore tape wrap or pararfilm. Agar requires a bit more of a sterile environment while working with. I've had bad luck unless I'm very very careful.
You rock I appreciate your info! Do you have any videos of what to look out for as far as what contamination on an agar plate looks like once you start getting growth ?
7:25 scary smiley face in syringe
Lol that’s awesome
Actually, on that last plate you looked at it may not be mold on the bottom, that may be a monokaryon mycelium, the thicker stuff looks like dikaryon mycelium. The mono won't fruit if you try to grow it out.
Thanks for the info! Im gonna try this tomorrow
Hell yeah, thanks PGT!
Dude I was literally looking for a video on this last night. Woke up to you uploading one lol
idk if it was intentional, but you were pretty on time with the song when you were tapping the syringe!
Hello PGT! So I live in the tropics and the temps are higher and pretty much stay to a now more than 7 to 10 degree flucation. WIll Mycelium grow even at my temperatures 76/87, Thanks for the help
Yes it will grow 🙏
Could you do another video like this but inside an SAB?
This channel is so cool. Thank you for the informative content!
I heard adult vibrating toys break up spore clumps like a champ...
For me it was less that the agar itself intimated me and more everything that might be neede d to use it successfully. You know, SABs,Flow hoods,etc. Now that I understand a bit more about sterilize procedure and how to make an affordable, effective SAB it doesn't seem so bad.
Name of the song please??? Thank you PGT for your videos I find it very helpful sensei keep kicking ass!!
This is awesome. I've been wondering what the point of the agar is (first time grower)
Pls keep the great videos coming! You are the best teacher on here for shroomies!
His got lots of good Stuff like, Psilocybin, Psychedelics, also have Shrooms, Spores Syringe, Micro-dose, Dmt, Lsd, Valium, Modafinil, XTC, Xanax, Grow kits, Grains, 1P-LSD, AL-LAD and lots more. He could guide you through anything you need..
Feelgrabz,,,,,...
Reach him via his Instagram handle or Telegram channel....
Great info.
Thank you.
Maybe a stupid question, but is there an advantage to putting it on agar as opposed to right in a grain bag?
Spore syringes are naturally dirty, and agar allows the ability to clean up the culture before introducing it to your grains
You should make a video on choosing a microscope i have been considering purchasing one lately to aid my mycological hobbies
I would also be very interested in this
So I definitely need to put my spores on agar first. I knew it had to be harder then just injecting spores. 🤣
Thanks alot for everything
Does PT come on here to answer questions? Why didn’t he eye loop the first dish on the left side? Where does one get those nice stainless racks for working in front of hepa?
This dude is great 👍
Love the jawn at 3:02 I’m from Philly so I caught it 😂😂
😄
How did you get the spores in the syringe?
Thank you
Mechanical agitation for the spore syringe? Tape it to the "back" massager for a few minutes?
I don't see why that wouldn't work 😄
Great video thanks for the info!
I made 40 cups and had 100% failure 😥 they’re all slimy and they all stink 😡 I’m pretty certain I squeezed to much juice in my agar. I’ll try my luck at cloning and maybe some spore agar. Thanks PGT
Great job.
Good Stuff! Thanks:)
😁🍄
Love the content
You Philly can you do a video explaining sectoring ?
"till i break this jawn apart". yup hes def from philly
Do you have any tips for identifying mycelium from anything else in a petri dish? Obviously orange or green is bad, but I have had petri dishes full of thick white fluffy stuff, which I imagine isnt mycelium but it is hard to know
Fluffy white is mycelium
Yo good lookin. I appreciate the content you put out . I’m on my first time doing this and I’m hoping for the best because I’m a beginner and want results but I’m also an avid and experienced grower of many things so I understand failure for growth.. it Looks like we have the same Agar., mine is called Rapid Rhizo.. hopefully this works, I’ll watch your video on Agar to grain bag I’m pretty sure I seen one on your video uploads.
What if you buy a spore syringe and you can't see any spores at all in there? Does that mean I've been scammed or is it possible there's spores in there somewhere? I've innoculated some agar just to check so will give that a week or so to see before kicking off lolz
So what this (and the comments) taught me is that the needle is unhelpful, adding resistance, retaining spore solution and just another contam opportunity.
If you’re not literally injecting something… Skip the needle and tap drops from the syringe. Or touch a sterile pipette to it for half a drop (still hundreds of spores).
Does anybody know how long the agar cups can be stored before they go bad out of the fridge?
about a month at room temperature. you probably can still use them longer than that, but for being optimal and efficient its best to use them sooner rather than later.
Where's the ketchup cups? I see you're moving on up. With subscribers too. Well done!
thank you great video
music taste goes hard homie
😄🍄
Why not just drip some drops right from the syringe onto the agar?
Hey pgt, Sorry if this question is answered in a different video but is there a reason your agar is blue? Is it just a different type of agar and is there any difference between the yellow agar I am used to seeing?
You can add food coloring to change the color! 😄
@@PhillyGoldenTeacher thank you man 👍🏻
Great video! May I ask where you found the metal platforms with holes? Seem like the perfect height.
does spore syringe to agar have better success rate than spore syringe to spawn bag?
There is potentially great success in both. The main thing is that spore syringes are inherently dirty, and agar is best to clean up the culture. So there is a more chance of complications with spore syringes in comparison
@@PhillyGoldenTeacher
Would you recommend: Spore Syringe > to Agar plate (cleansing of culture) > to Spawn Grain?
Amazing 🤩🤩
This isn’t the agar that you show how to make in the video. What is the recipe for this one?
Dude. I need help with my agar. It keeps getting contaminated. Don’t know if it’s the syringe or my area. But with the same syringe I inoculate a bag and the mycelium takes off
Should I just take some mycelium from the bags and put those to agar
I swabbed some spores, injected some spores onto agar and so far nothing but nothing. It looks like the liquid from the syringe just spread over the agar.
I did not see spores in my syringe? Thx. KC
They are microscopic. You won't always see spores in syringes, but hopefully they are there! 🙏
once the spores are on the agar, whats the best/ideal conditions for the agar? room temp in the dark ect.. going to give agar a go with all the info learned . thanks PGT
Room temp is fine, anywhere between 75 and 80 F is optimal.
@@vectionnoitcev2836 thanks, how long in these conditions would one expect to see some growth?
@@nnguyen3482 Anywhere from 4 days to a week. It's been 4 days since I inoculated dishes and one is showing signs this morning. The timing of your question is perfect! Two dishes were done from a spore print and six with spore syringes, 2 dishes for each variety. The spore print wins the race so far. I put 4 little drips from the spore syringes on each dish just to be sure but no activity as of yet...
@@vectionnoitcev2836 haha your timing is on point too. I'm going to try both ss/print later today. thanks for the help
@@vectionnoitcev2836 Hi! Did your syringed spores ever pop?
Can you drop spores on the plate and spread them with a flamed loop and skip the shot glass step? Hybrid of your 3 techniques.
Just wondering... do you ever inoculate slants directly from a LC syringe? Vs LC Syringe to Petri Dish to Slant
Can you just use sterilized cotton swabs on the spore print and swab them across agar or would that be wasteful?
You can use a sterile swab to transfer
I’m new to mycology. Do I store the LC with the needle on or off the syringe after inoculating the agar? Do I recap the syringe? Love the video!