here is another tip: with the syringe cap on tight pull the plunger a short distance and quickly let it go, it will create a shockwave & disperse the spore clumps.
Newbie here and your content is great!! I’m learning sooo much. Thank you for explaining everything extensively. After watching other channels and finally hearing you explain things to where I understand it - you hear many “ohhhhhhhs” coming out of me. Your channel is quickly a favorite. Thanks for all the great content!
Hello PGT, really just wanted to express my gratitude for all your hard work in the videos that you're doing, I'm a newbie at this only about 3 weeks into it but I'm so excited about it, I don't know why I'm so drawn to it, I think you're doing such a wonderful job and I really just wanted to convey that, thank you so much.
Sensei, thank you, thank you, thank you! I'm starting my journey in mycology and microdosing to help my grandson who suffers from epilepsy, Crohn's, and depression. Of all the DIY videos I've watched, yours call to me. I've inoculated agar plates and made brown rice mini-jars (pint). Wish me well!
your such an amazing woman and grandmother. I hope your seeing amazing results from the plant medicine to help your grandson. I truly believe mother nature gave us everything we need. Blessings to you and your grandson.
As a medic I've found that if you pull back on the syringe before you push the solution out you have much more control over how much comes out during that first push.
You can place a just slightly damp alcohol wipe over the end first. As an addict in recovery I can confirm that it's much easier if you pull back first
@ 5:08 you mention the first depression of the syringe causes way too much to come out. I was hoping you were gonna say something. I work in the medical field and our saline flushes do the same thing. To resolve this, simply pull back on the syringe, it breaks the stopper free and then smoothly moves forward. ;-) But be careful not to pull it backwards out of the body, just a tiny bit to free it up, twisting sometimes helps too.
3 videos in one weekend! You the man 🤙🏼, we appreciate all your efforts. I've been growing cannabis for 10 years and now with the help from you, and members of the mycology community on TH-cam and reddit, I've been slowly buying all my supplies over the past few weeks while I study the craft before I start. This will probably never get read but thanks brother ✌🏼🍄 EDIT: 8 months later and I’ve grown pounds of TATs 😂🤙🏽 (side note) I also just had my very first contamination 🤙🏽
I could say the exact same thing as Psychonaut. Been growing herb for years and thanks to Teacher and a few other, my fruiting room is ready and I'm waiting for my spores to come in. Can't wait.
Thanks for the content! Not trying to sound unappreciative with my last comment - it's just helpful to get an idea of the process/order of events when working with an SAB because it can confusing trying to parse together when to go out of the box then back in to flame sterilize the needle etc without disrupting the still air. Been watching lots of different videos to try to piece it together, this one helps a lot!
Idk if he ever says so in the video, but definitely store your agar dishes/cups upside down before and after inoculating to cut down on contam caused by condensation mushlove
I suggest leaving out the shot glass and picking up a 5ml graduated cylinder. It helps me to avoid using too much solution. 1ml will innoc dozens of plates. Also, a pipette gives much more control over the single drop. [5ml grad cylinder w/ glass pipette $6.99 @ Amazon] Same equipment can be used to transfer LC to plates or slants.
Thanks a ton PGT. Thanks in a big part to you, I just received my 1st mycology research kits today. Your lessons have been invaluable in my learning and planning . Now I just need to pour those 200 agar plates. :) Thank you.
Please make a video about how you made your still air box. I'm getting into the hobby (collecting my tools and supplies now) but have yet to see a good how-to on a good still air box and yours seems luxurious as you have room for a camera inside. ;)
Well darn. I could have used this video yesterday. Searched a bit and this pops up today. I used the direct inject method onto the agar. Shaking up all the spores was the hard part. Didn't think to add a slight touch of air to help the solution shake better. Figure the cotton swab method will work best.
You can stack your plates right after pouring, the heat will rise through the plates and remove condensation from the lower plates. the best method to eliminate condensation completely is to leave your plates in front of the flowhood for 2-3 days after pouring. the laminar flow slowly makes its way through the plates and dries up condensation.
I gave mine a quick shake, and was able to get droplets to condense, to see spires growing, but now I gotta leave it tilted or upside down till the water dries up.
You rock I appreciate your info! Do you have any videos of what to look out for as far as what contamination on an agar plate looks like once you start getting growth ?
Thanks for this, I prefer using Agar cups, but once I'm done growing for a season, I put everything away. All I have left is spore prints in storage. Going from spores to agar allows me to skip a step.
Thank you bro, just getting started with some lions mane, King Oyster, and others, I picked up some innoc loops and sterile swabs for a few bucks on Amazon, will be swabbing in a couple of days, thanks for all your great vids. After agar grows out, will transfer to agar, and l.c. and see how things progress, then onto my masters mix, and grain/ verm mixes to see which varieties grow best on each medium...thanks again.Pgt
I've been using zip lock sandwich bags to store my plates in, after micropore tape wrap or pararfilm. Agar requires a bit more of a sterile environment while working with. I've had bad luck unless I'm very very careful.
What if you buy a spore syringe and you can't see any spores at all in there? Does that mean I've been scammed or is it possible there's spores in there somewhere? I've innoculated some agar just to check so will give that a week or so to see before kicking off lolz
Do you have any tips for identifying mycelium from anything else in a petri dish? Obviously orange or green is bad, but I have had petri dishes full of thick white fluffy stuff, which I imagine isnt mycelium but it is hard to know
Hello PGT! So I live in the tropics and the temps are higher and pretty much stay to a now more than 7 to 10 degree flucation. WIll Mycelium grow even at my temperatures 76/87, Thanks for the help
Great videos! I need to get better at spotting contamination. I thought that plate was good. I'm gonna check more videos , you probably have one about spotting contamination already.
Hi my name is Lance. I am a new grower and I just watched one of your videos and I like how simple your videos are been watching a lot of other videos trying to learn the mycology. Just wanted to say thanks and I’m going to watch more of your videos
Only had one successful grow 2 summers back. This summer i had bought all this stuff, going for some oats jars and pf tek jars... I used agar and thought I had some good plates, they looked good. I cut up the agar, plop it into the jars, they colonized quick. Then i opened them to mix w coco coir and peeee yew smelled rancid... Slightly yellowish. Had to dump it. Gonna go at it again. I pressure cook everything lol! I don't have a good, use a SAB.. something got in it. Well spring is here, time to try again
I’m so new to this. I have a few questions. 1. What do I do with the leftover syringe? 2. after the inoculation process is complete, what comes next and then after that? I need help. 😅
What I'm looking for is taking your inoculated spore agar and making a culture in a syringe from a slice. Is this possible and is there a video with this !!! Thank you Patrick McG. Rookie mycologist self taught journey. Please reply I would be very grateful. Have a wonderful day. 😊Mush Love !!
His got lots of good Stuff like, Psilocybin, Psychedelics, also have Shrooms, Spores Syringe, Micro-dose, Dmt, Lsd, Valium, Modafinil, XTC, Xanax, Grow kits, Grains, 1P-LSD, AL-LAD and lots more. He could guide you through anything you need..
about a month at room temperature. you probably can still use them longer than that, but for being optimal and efficient its best to use them sooner rather than later.
Hey pgt, Sorry if this question is answered in a different video but is there a reason your agar is blue? Is it just a different type of agar and is there any difference between the yellow agar I am used to seeing?
Why is it whenever I try the loop method I just get an off white almost transparent goop wherever I have gone with the loop? Its very annoying, and I have tried different vendors.
Love your videos bro! Just wanted to add my own cheap and easy inoculation loop I use. I just got a black head popper from the dollar store 😂😂😂, all stainless steel and can be put in a PC to sterilize if necessary
Actually, on that last plate you looked at it may not be mold on the bottom, that may be a monokaryon mycelium, the thicker stuff looks like dikaryon mycelium. The mono won't fruit if you try to grow it out.
Does PT come on here to answer questions? Why didn’t he eye loop the first dish on the left side? Where does one get those nice stainless racks for working in front of hepa?
Okay-super new to this and still learning-what’s the point of doing spore syringe to agar? To rule out contam in the syringe before inoculating? Also do you need to leave at room temp or slightly warmer for best results.
Is warmer better than cooler? I am watching vigorous Mycellia vs. Bacterial Muck. Which grows better at which temperature - if it is clearly a failed plate that needs plate to plating?
Is it possible that spores doesn't need oxygen to grow? you have it in sealed petri dish & it make us very curious how to actually get that blue agar for growing mycelium. I think this the cleaner method, which i would like to attempt. Thank you for sharing this information with us.. had no idea if mushroom spores can grow in agar dish.
Why would one choose to create more opportunity for infection? IE. Squirt into shot glass, then to loop or swab then to agar? Seems to me the less transfers the better - drop from syringe to agar, done?
Will condensation ruin your agar? I just received new plates in the mail and brought them in the house and now I have moisture droplets on top. Should I store in fridge? Can I get rid of the moisture or are plates ruined?
What to do if your climate is tropical and temperatures are higher than 86°F / 30°C? Where I am it can get above 92°F / 34°C. What happens to the agar and the mycelium? Would you have to do some sort of climate control or use a converted fridge to keep it at lower temps in the 72°F/ 22°C to 83°F/ 28°C range?
I saw your Martha tent. Do you have any videos for still air boxes or do you have a entire room of clean space? I ha e a box with mat Heater. Ive injected l. Getting mycelium, but am not sure about mixing my grow bag or changing the temp. Im just getting started, Ill check out more videos
I'm about 2 years into mycology and starting to up my game now. I have a question about storage. Where should I be storing these agar plates after inoculation? In the fridge, on a shelf, keep it in the still air box?
Hi, thanks for the tutorial. I was wondering how this would work in a still air box. Would you still get away with the shot glas and cottonswab and would you recommend to use a new one for each plate or is the shotglas a nogo without the laminar flow hood?
provided you properly sanitized the area and dont have any fans going to move air around you it SHOULD be fine as long as you still try to minimize open air contact time... but as always everything you don't do perfectly in this hobby is going to increase your chances of failure on some level.
"Break this JAWN Apart" Philly is Really in the house.
here is another tip:
with the syringe cap on tight pull the plunger a short distance and quickly let it go, it will create a shockwave & disperse the spore clumps.
Nice, thanks
Here's a tip: throw soil on top of your sub when your done fruiting it to make a indoor compost bin. Lots of fun
@@Enochulate88 after harvest? or?
Hey, I’ve recently had my first two flushes ever and want to thank you man, I’ve got knowledge from you that I’ll hold forever. Thanks alot
Newbie here and your content is great!! I’m learning sooo much. Thank you for explaining everything extensively. After watching other channels and finally hearing you explain things to where I understand it - you hear many “ohhhhhhhs” coming out of me. Your channel is quickly a favorite. Thanks for all the great content!
Another tip for controlling drops is just squeezing the sides rather than pushing the plunger. Thanks for what you do for us PGT!
That awesome, you just use it as a pipette. Genius.
Syringes are hard plastic.
That can introduce air back into the syringe
@@fungdark8270 you do it without the syringe
Yeah Bro..
You are a Genius..
Hello PGT, really just wanted to express my gratitude for all your hard work in the videos that you're doing, I'm a newbie at this only about 3 weeks into it but I'm so excited about it, I don't know why I'm so drawn to it, I think you're doing such a wonderful job and I really just wanted to convey that, thank you so much.
Sensei, thank you, thank you, thank you! I'm starting my journey in mycology and microdosing to help my grandson who suffers from epilepsy, Crohn's, and depression. Of all the DIY videos I've watched, yours call to me. I've inoculated agar plates and made brown rice mini-jars (pint). Wish me well!
You sound like an amazing grandmother! Hope all is going well :)
haha nuts to see an older woman make drugs
Much love to you and your grandson. Hope you're both doing well.
your such an amazing woman and grandmother. I hope your seeing amazing results from the plant medicine to help your grandson. I truly believe mother nature gave us everything we need. Blessings to you and your grandson.
As a medic I've found that if you pull back on the syringe before you push the solution out you have much more control over how much comes out during that first push.
But you risk letting contams enter the syringe probably
Adding on to my previous reply, if done in a still air box it would probably be kinda safe
You can place a just slightly damp alcohol wipe over the end first.
As an addict in recovery I can confirm that it's much easier if you pull back first
Risking pulling in contaminates in the air? Why risk it
I purge that first push into a shot glass, its all dead spores anyway you just cooked um.
The confidence wrapping with the parafilm shows pure practice. ❤
@ 5:08 you mention the first depression of the syringe causes way too much to come out. I was hoping you were gonna say something. I work in the medical field and our saline flushes do the same thing. To resolve this, simply pull back on the syringe, it breaks the stopper free and then smoothly moves forward. ;-) But be careful not to pull it backwards out of the body, just a tiny bit to free it up, twisting sometimes helps too.
3 videos in one weekend! You the man 🤙🏼, we appreciate all your efforts. I've been growing cannabis for 10 years and now with the help from you, and members of the mycology community on TH-cam and reddit, I've been slowly buying all my supplies over the past few weeks while I study the craft before I start. This will probably never get read but thanks brother ✌🏼🍄
EDIT: 8 months later and I’ve grown pounds of TATs 😂🤙🏽 (side note) I also just had my very first contamination 🤙🏽
Dude just go for it! Learn on the job :)
Best. Hobby. Ever. 🤙💚
I could say the exact same thing as Psychonaut. Been growing herb for years and thanks to Teacher and a few other, my fruiting room is ready and I'm waiting for my spores to come in. Can't wait.
F TH-cam and Reddit. If you want info on cultivating, foraging, or anything that has to do with mycology. Then go to The Shroomery.
@@michaelcoleman5922 Where did you get your spores?
Having a shitty day... came home took some food open youtube and new video from PTG! Now my day is a lot better :)
Love learning from someone so knowledgeable, and I absolutely love your video style. You put a lot of work into this channel, good freaking job my man
I'm glad you are feeling better 💜
Man, I wish this was posted yesterday at this time! I just did the squeeze one drop method. Should have done the streak method. Oh well, thanks PGT!
same here
I have way more than a couple drops
Most amazing coincidence this month! Love your videos and literally this weekend got plates to do SS to agar, glad I waited you read my mind!
So good. You make info that seems incomprehensible simple. I’m stoked to start working with agar. 👍
I'm glad I could help. Good luck starting your journey with agar! 😊
Thanks for the content! Not trying to sound unappreciative with my last comment - it's just helpful to get an idea of the process/order of events when working with an SAB because it can confusing trying to parse together when to go out of the box then back in to flame sterilize the needle etc without disrupting the still air.
Been watching lots of different videos to try to piece it together, this one helps a lot!
Idk if he ever says so in the video, but definitely store your agar dishes/cups upside down before and after inoculating to cut down on contam caused by condensation mushlove
i remember learning this in my freshman year of highschool in biology 😂
@@Yokoname that's way cool! I unfortunately had to find this out the hard way I lost all my first agar cups to contam bc they weren't upside down
Thank you for this info! I didn’t partake in any science fairs since I was usually hiding under classroom portables high as a 🦒 a$$ 😅
your whole channel is a blessing!
Always nice to see a good education video from u mate!
I suggest leaving out the shot glass and picking up a 5ml graduated cylinder. It helps me to avoid using too much solution. 1ml will innoc dozens of plates. Also, a pipette gives much more control over the single drop. [5ml grad cylinder w/ glass pipette $6.99 @ Amazon] Same equipment can be used to transfer LC to plates or slants.
I like the sound of this method. How much liquid do you drop onto each plate?
@@ChrisMosner
Literally 1 drop or less is all that will be needed.
Wow! Vegan ham candy! This video makes me so happy! Thank you!
I'm just a beginner and this video was extremely helpful. Thank you.
literally what I was looking for, just ordered spore syringes yesterday and was wondering if I could start messing with agar, thanks!!!
Thanks a ton PGT. Thanks in a big part to you, I just received my 1st mycology research kits today. Your lessons have been invaluable in my learning and planning . Now I just need to pour those 200 agar plates. :) Thank you.
thank you great video
Please make a video about how you made your still air box. I'm getting into the hobby (collecting my tools and supplies now) but have yet to see a good how-to on a good still air box and yours seems luxurious as you have room for a camera inside. ;)
Well darn. I could have used this video yesterday. Searched a bit and this pops up today. I used the direct inject method onto the agar. Shaking up all the spores was the hard part. Didn't think to add a slight touch of air to help the solution shake better. Figure the cotton swab method will work best.
Mush love been following you since high school love your work thanks so much for your inspiration!
Many Thank yous dear Jack! Looking forward to seeing where the peitre dish goes next!! 😉
😄
I can see some white stuff in one of my bags. What now?? Thanks you!
@@karinchristensen9809 Well?
What Happened Next?
No matter what I do, I get massive condensation in my agar plates. Any tips? Thanks pgt & glad to have you back.
You can stack your plates right after pouring, the heat will rise through the plates and remove condensation from the lower plates. the best method to eliminate condensation completely is to leave your plates in front of the flowhood for 2-3 days after pouring. the laminar flow slowly makes its way through the plates and dries up condensation.
I gave mine a quick shake, and was able to get droplets to condense, to see spires growing, but now I gotta leave it tilted or upside down till the water dries up.
You rock I appreciate your info! Do you have any videos of what to look out for as far as what contamination on an agar plate looks like once you start getting growth ?
Thanks for this, I prefer using Agar cups, but once I'm done growing for a season, I put everything away. All I have left is spore prints in storage. Going from spores to agar allows me to skip a step.
How can you tell the difference between mycelium and mold? They look so similar except the mold wasn't as thick looking
Yet another great video Teacher! You show so many great ways to get shrooms started that it's nearly impossible to fail! Thanks again!
Great vid , I was wondering how to put spores to agar 💯💪🏾 you answered all my questions
Hell yeah, thanks PGT!
Homeade ingenuity brother, been following your ways for a minute with great luck forsure (: lol keep up the good work!
Thanks for the info! Im gonna try this tomorrow
Thank you bro, just getting started with some lions mane, King Oyster, and others, I picked up some innoc loops and sterile swabs for a few bucks on Amazon, will be swabbing in a couple of days, thanks for all your great vids. After agar grows out, will transfer to agar, and l.c. and see how things progress, then onto my masters mix, and grain/ verm mixes to see which varieties grow best on each medium...thanks again.Pgt
I've been using zip lock sandwich bags to store my plates in, after micropore tape wrap or pararfilm. Agar requires a bit more of a sterile environment while working with. I've had bad luck unless I'm very very careful.
This channel is so cool. Thank you for the informative content!
What if you buy a spore syringe and you can't see any spores at all in there? Does that mean I've been scammed or is it possible there's spores in there somewhere? I've innoculated some agar just to check so will give that a week or so to see before kicking off lolz
How did you get the spores in the syringe?
Do you have any tips for identifying mycelium from anything else in a petri dish? Obviously orange or green is bad, but I have had petri dishes full of thick white fluffy stuff, which I imagine isnt mycelium but it is hard to know
Fluffy white is mycelium
I knew I forgot something . Well I'm learning . Thank you for sharing your knowledge . It's literally gold ✨️ .
Hello PGT! So I live in the tropics and the temps are higher and pretty much stay to a now more than 7 to 10 degree flucation. WIll Mycelium grow even at my temperatures 76/87, Thanks for the help
Yes it will grow 🙏
idk if it was intentional, but you were pretty on time with the song when you were tapping the syringe!
Great videos!
I need to get better at spotting contamination. I thought that plate was good. I'm gonna check more videos , you probably have one about spotting contamination already.
Hi my name is Lance. I am a new grower and I just watched one of your videos and I like how simple your videos are been watching a lot of other videos trying to learn the mycology. Just wanted to say thanks and I’m going to watch more of your videos
Thank you lance, I wish you the best on your journey! 😁🍄
Dude I was literally looking for a video on this last night. Woke up to you uploading one lol
Only had one successful grow 2 summers back. This summer i had bought all this stuff, going for some oats jars and pf tek jars... I used agar and thought I had some good plates, they looked good. I cut up the agar, plop it into the jars, they colonized quick. Then i opened them to mix w coco coir and peeee yew smelled rancid... Slightly yellowish. Had to dump it. Gonna go at it again. I pressure cook everything lol! I don't have a good, use a SAB.. something got in it. Well spring is here, time to try again
I’m so new to this. I have a few questions. 1. What do I do with the leftover syringe? 2. after the inoculation process is complete, what comes next and then after that? I need help. 😅
Just wondering... do you ever inoculate slants directly from a LC syringe? Vs LC Syringe to Petri Dish to Slant
Could you do another video like this but inside an SAB?
With the cotton swab that had all those spores left on there do you just throw it away? Or is there something you can do with it?
What I'm looking for is taking your inoculated spore agar and making a culture in a syringe from a slice. Is this possible and is there a video with this !!! Thank you Patrick McG. Rookie mycologist self taught journey. Please reply I would be very grateful. Have a wonderful day. 😊Mush Love !!
If you add the agar growth to a 2-3lb grow bag, would you still need to add additional spore syringe or would it already be enough?
7:25 scary smiley face in syringe
Lol that’s awesome
Pls keep the great videos coming! You are the best teacher on here for shroomies!
His got lots of good Stuff like, Psilocybin, Psychedelics, also have Shrooms, Spores Syringe, Micro-dose, Dmt, Lsd, Valium, Modafinil, XTC, Xanax, Grow kits, Grains, 1P-LSD, AL-LAD and lots more. He could guide you through anything you need..
Feelgrabz,,,,,...
Reach him via his Instagram handle or Telegram channel....
Maybe a stupid question, but is there an advantage to putting it on agar as opposed to right in a grain bag?
Spore syringes are naturally dirty, and agar allows the ability to clean up the culture before introducing it to your grains
This is awesome. I've been wondering what the point of the agar is (first time grower)
Does anybody know how long the agar cups can be stored before they go bad out of the fridge?
about a month at room temperature. you probably can still use them longer than that, but for being optimal and efficient its best to use them sooner rather than later.
What did you do to sanitize, disinfect that shot glass ? Just hot soap and water, dried with a paper towel?
Mechanical agitation for the spore syringe? Tape it to the "back" massager for a few minutes?
I don't see why that wouldn't work 😄
Great info.
Thank you.
Hey pgt, Sorry if this question is answered in a different video but is there a reason your agar is blue? Is it just a different type of agar and is there any difference between the yellow agar I am used to seeing?
You can add food coloring to change the color! 😄
@@PhillyGoldenTeacher thank you man 👍🏻
Why is it whenever I try the loop method I just get an off white almost transparent goop wherever I have gone with the loop? Its very annoying, and I have tried different vendors.
Love your videos bro! Just wanted to add my own cheap and easy inoculation loop I use. I just got a black head popper from the dollar store 😂😂😂, all stainless steel and can be put in a PC to sterilize if necessary
Is there any need to put a needle on the syringe for anything done here. Could you not just drip it straight from the syringe?
the needle helps you get more control on squirting, but yes you can just use the syringe without the needle.
Wow when you were tapping the spore syringe on your finger it exactly matched the music in the video
😄
Actually, on that last plate you looked at it may not be mold on the bottom, that may be a monokaryon mycelium, the thicker stuff looks like dikaryon mycelium. The mono won't fruit if you try to grow it out.
Does PT come on here to answer questions? Why didn’t he eye loop the first dish on the left side? Where does one get those nice stainless racks for working in front of hepa?
Okay-super new to this and still learning-what’s the point of doing spore syringe to agar? To rule out contam in the syringe before inoculating? Also do you need to leave at room temp or slightly warmer for best results.
Is warmer better than cooler?
I am watching vigorous Mycellia vs. Bacterial Muck.
Which grows better at which temperature - if it is clearly a failed plate that needs plate to plating?
Is it possible that spores doesn't need oxygen to grow? you have it in sealed petri dish & it make us very curious how to actually get that blue agar for growing mycelium. I think this the cleaner method, which i would like to attempt. Thank you for sharing this information with us.. had no idea if mushroom spores can grow in agar dish.
For how long can you storage full colonized agars? You storage this iun the fridge, in a room temperature?
Why would one choose to create more opportunity for infection? IE. Squirt into shot glass, then to loop or swab then to agar? Seems to me the less transfers the better - drop from syringe to agar, done?
Will condensation ruin your agar? I just received new plates in the mail and brought them in the house and now I have moisture droplets on top. Should I store in fridge? Can I get rid of the moisture or are plates ruined?
What to do if your climate is tropical and temperatures are higher than 86°F / 30°C? Where I am it can get above 92°F / 34°C. What happens to the agar and the mycelium? Would you have to do some sort of climate control or use a converted fridge to keep it at lower temps in the 72°F/ 22°C to 83°F/ 28°C range?
I’m new to mycology. Do I store the LC with the needle on or off the syringe after inoculating the agar? Do I recap the syringe? Love the video!
Great video thanks for the info!
Nice we’re can I get the products at
I saw your Martha tent. Do you have any videos for still air boxes or do you have a entire room of clean space? I ha e a box with mat
Heater. Ive injected l. Getting mycelium, but am not sure about mixing my grow bag or changing the temp. Im just getting started, Ill check out more videos
This applies to liquid culture as well right? Like LC syringe to agar plates should work fine just like with spore syringe?
Where is best place to store plates after fully colonized? How long will plates be viable?
Name of the song please??? Thank you PGT for your videos I find it very helpful sensei keep kicking ass!!
Where do you store your plates? In a cooler or a small refrigerator?
Great video! May I ask where you found the metal platforms with holes? Seem like the perfect height.
Can the spore syringe method be used the same way with a liquid culture syringe?
I'm about 2 years into mycology and starting to up my game now. I have a question about storage. Where should I be storing these agar plates after inoculation? In the fridge, on a shelf, keep it in the still air box?
Can you make your own sterile swabs by putting Q-tips in the PC?
Whats the temperature we should keep these agar plates after transfer?
You should make a video on choosing a microscope i have been considering purchasing one lately to aid my mycological hobbies
I would also be very interested in this
Hi, thanks for the tutorial.
I was wondering how this would work in a still air box.
Would you still get away with the shot glas and cottonswab and would you recommend to use a new one for each plate or is the shotglas a nogo without the laminar flow hood?
provided you properly sanitized the area and dont have any fans going to move air around you it SHOULD be fine as long as you still try to minimize open air contact time... but as always everything you don't do perfectly in this hobby is going to increase your chances of failure on some level.
What can you do with culture still in the syringe can u put it in the fridge
Yes you can 🙏🍄
Can you drop spores on the plate and spread them with a flamed loop and skip the shot glass step? Hybrid of your 3 techniques.
Aye teach, would this process be the same
With liquid culture syringes?
Yes you can!
music taste goes hard homie
😄🍄