I don't do anything with mycology but randomly got your broke boi tek video recommended and binged this channel since. I have OCD and mild aspergers and get stressed to hell when following instructions/ "how to's" in general but something about this is just so calming on a visceral level. Thx man.
Heya. Wondered if you’d like a tip (which to be fair you might already know). Get a chill tune with a swaying beat (kind of like the background music here) and learn your instructions to the tune. Then when you carry out the instructions move to the beat and it’s like you’re dancing them out. It’s very calming and helps both my neurological spasms disappear and my partner’s Aspergers concentration levels heighten and he de-stresses. Also what others have said regarding psilocybin (microdosing) helps Aspergers stress for people I know but it’s not for everyone. Food for thought though. Literally. Make the instructions into a song and the movements into a dance. It becomes graceful and natural and calming.
Very few channels can explain this craft right as a whole. From spore, agar, fruiting conditions and more. Even less can actually teach. Thank you for being one of the rare gems that can explain in a way many can learn. One hell of teacher. A golden standard of one if you will lmao. Mush love ✌
Transferring clean, snow white mycelium onto a plate of virgin agar is one of the things I enjoy most about working with mycelium. Waiting for your LC video now...thanks for helping all of us improve our skills, PGT!
@@jroc09 In mycology, there‘s ‚contam(ination)‘, and ‚iso(lation)‘. Isolating a certain growth from a clean culture can provide certain characteristics that you want to isolate, e.g. especially strong growth for fast colonization of substrate.
Instead of throwing out the one with a small spot, do the transfers then put a little h2oo on the spot then cut it out. I have also poured h2oo into the agar up to the the mycelium, wait for the bubbling to stop and pour it off. It pours off the contam with it. Have saved many agar plates like this and grown good mushrooms.
Just inoculated some grain jars, and I just gotta say Teacher....I have always been Interested in the thought of trying to do this myself, and it was YOUR videos that gave me the final push to actually continue and start to teach myself about my new journey in mycology! I'm completely green...and im looking forward to making the many learning mistakes I have in my short future🤣 but I just wanted to thank you for all your efforts, in making all these AWESOME videos that have taught so many like me to just stop worrying and GET INTO IT! I'm trying to not be like so many who rush and make newbie mistakes along the way with dreams of how fast and easy their first flush will be🤣 but we will see how I do...excitedly watching 10 jars of b+ on brown rice grains slowly start to turn fuzzy! I bought spores instead of a liquid culture syringe though...so I've already made my first mistake😂 sorry for the long comment, but I just needed to thank and tell you that YOU'RE the MAN! And NOW? here I am learning about agar from you! absolutely making a difference in the world my man! Mush love
I did my transfer just this past saturday. Unfortunately this video didnt come out in time for help. But all of them are doing GREAT! nice and healthy and fluffy white myc. Got some Golden Teachers and some Tidal Wave
If u put ur cups in ur oven when ur pouring ur agar an putting the lids on in the oven will cut down on contam .350 works great to blows the contam away , From mush love ❤️ The Astro way is a great channel too , Love ur style great job on ur channel an ur teaching nods like me 👍
U can put plates in the fridge and use them a month later probably even two months but for actual long term storage of mycelium in a fridge you should use culture slants
0:19 the top corner growth is not mycelium? Also, once you make the transfer from cup 1 to cup 2, do you discard cup 2 or can use that for grain transfer as well?
Question! Do you sterilize your agar solution and then pour into the disposable ramekins? Or do you sterilize the agar and ramekins together like in the pastywhyte tek? I assume the ramekins would melt in a PC but you never know 🤷🏻♂️
I notice that you don't use petri dishes covered with parafilm. Would that disposable container you use still be fine? I feel like air might get inside even when they are closed since they are made of thin plastics. Thank you for your precious postings
What's this? A youtuber with affiliated links in the description that actually tells you they make a commission off of them? I'm thrilled! Love your vids.
If you only have spots what I have done and it worked was take a small piece of paper towel, a little bigger then the spot, soak it with hydrogen peroxide, cover and let sit. I found the next day it was killed and a few days later the mycelium grew over it. If the whole thing is green spray the h2 oo on the whole thing and see what happens. At that point it can’t hurt. Let me know how it goes.
When making an agar to agar transfer, does it matter whether the transfer is placed agar side up or agar side down? I am subscribed, how does one get to the discourse community. Outstanding video, PGT. Mush appreciated.
Why not immerse everything in alcohol especially the agar cups and lids, then quickly let dry before you pour the agar? I saw the video of working with the cups in front of the open oven on 250 degrees, but I don't think that's enough to sterilize them. Maybe that's why you got contaminated medium?
Yes, depending on conditions, it can take 6 weeks or longer from a spore syringe to get minor mycelial growth. This method fast-forwards you to that point.
I noc'd spores to grain 31 march, it will be ready to xfer next week. Ill give agar a shot in the next coupla weeks to test a syringe that didnt do shit.
What Are These plastic containers you are using instead of Petri dishes? It seems they also don’t require any parafilm to be sealed. Pretty handy if that works 😉
They are condiment cups for anyone else wondering. They’re pre-sterilized and don’t require parafilm. Also super cheap. Only downside is they don’t look as visually appealing when the mycelium is fully grown out as it does on traditional Petri dishes.
Hi PGT. Always love your work. Thank you! Quick one: when transferring a slice of culture from one dish to another, I notice some people put down the small wedge upside down in the second agar dish. Others put down the wedge right side up. Does it matter, and why? Kind regards, and thanks in advance.
@@PhillyGoldenTeacher Oh! gee, back in my heyday (on "the forums") when I was in college I would have sent you a couple stir bars. You can get a basic kit on Amazon these days for ~$50 with plate, bars, and extractor. My only problems with LC have been ignoring gas exchange and stir bars; or these days forgetting the stir bars. I really admire you passing this knowledge on to a new generation of amateur mycologists; my hat's off to you sir.
Sensei! A Question: After multiple transfers were able to get rid of contam from spores, mycel stops growing after about a week of growth (size of dollar coins roughly). Multiple transfers, same problem. Is it moisture? Heat? No contam visible, it just...stops. Can anyone offer advice?
Hi! I just ordered a kit of lion’s mane…I want to take the spores and then eat the mushrooms, I want to know the taste and the texture!!! Then will do the agar, and then the grain n the final touch..long n nice process…one step at the time…good luck to us!!,
I'm a novice, and ive learned alot from this channel. But I don't understand why multiple transfers are needed. Wouldn't that only increase the chance for contamination if the mycelium is clean after the first transfer? Or is it just likely there are contams embedded into the mycelium itself until multiple transfers of clean mycelium are done? Thanks for the educational content philly!
How do you make your agar in the cups, Do you poor it and put it in the pc, I do that but I get too much moisture in the cups. What is the right way to do this. Very much appreciated for your response. Thanks.
just trying out my first agar plates with some oyster spore . Mycilium is growing like wildfire although ive noticed some yellowing on a couple plates . mycilium piss or contamination ?
That small spot looked like stray mycelium.... they usually happen when there is water dripping onto your spores ill get runoff that starts mycelium on the sides of the cup, but they eventually converge and form rhizomorphic mycelium!
how come you can transfer from contaminated agar and not contaminate the transferred plate but you cannot open a dub tub thats contaminated wouldn't the same rules apply here with the contaminated spores in the air?
How early can I transfer mycelium from a spore swab to agar? I have some specks that are in the infancy of colonizing my agar dish can I transfer the myco that looks promising I have a few strains of Rizo growth but these are small about the thickness of a nickel just little strings the growth on some specks are smaller than a pencil eraser in circumference I’m trying to catch the fast growing myco early by cutting out the spots of rapid growth compared to the rest of the dish before it all combines I guess how early is too early?
Pgt! I love your channel, you’ve helped me and all of us tremendously thank you! I have a question. I followed your agar recipe, and transferred some agar from another plate just like you did in this video, and almost 2 weeks later I still have no growth in them. I think I might have not boiled the mixture long enough or I might have put too much food coloring. Would either of those be to blame?
@@DEGONDVR From what I've read in his and other comments, yes it's to promote growth. After the 3rd or 4th transfer you can apparently trust it to rigorously spread
Im learning here . Is it possible to cut off a bit of mycelium thats just colonised an all in 1 grow bag and put it in a new all in 1 bag to colonised that ?
If you made the agar pots and don't have a hood would it be ok to spray some iso alcohol on it to kill bacteria and leave for storage? (With lid on).. one last thing I'm going to try some clove oil in my agar mix would that work... thanks
Curious about timing. Total newbie here-sorry if it’s basic... Does a spore to grain take about the same time as a spore to agar in terms of mycelium development and growth? My guess is spore to agar works faster?
How long does a agar plate usually take to completely colonize? I inoculated played 2 weeks ago and they seem like they’re barely colonizing. They’re growing in very thin. Is it bad if I just let the agar sit there to see if it’ll colonize more ? I don’t have any contam & I’m just confused at this point. Could it possibly be bad genetics? I’m a amateur btw only 2-3 months into this hobby 😅
Bactertial agar is only good in special cases for extremely dirty cultures like wild harvested or special cases. It can create resistance using it often. Generally speaking, it's not something that should be used unless necessary.
I don't do anything with mycology but randomly got your broke boi tek video recommended and binged this channel since. I have OCD and mild aspergers and get stressed to hell when following instructions/ "how to's" in general but something about this is just so calming on a visceral level. Thx man.
You should try some mushrooms as a therapy and report back
I agree, very cool and chill dude here.
@Aco Znas94I ...psychotic..??
Jordan Babcock Psychotic mushrooms are what you hallucinate on a bad trip? 😝
Heya. Wondered if you’d like a tip (which to be fair you might already know).
Get a chill tune with a swaying beat (kind of like the background music here) and learn your instructions to the tune. Then when you carry out the instructions move to the beat and it’s like you’re dancing them out.
It’s very calming and helps both my neurological spasms disappear and my partner’s Aspergers concentration levels heighten and he de-stresses.
Also what others have said regarding psilocybin (microdosing) helps Aspergers stress for people I know but it’s not for everyone. Food for thought though. Literally.
Make the instructions into a song and the movements into a dance. It becomes graceful and natural and calming.
I'm pouring my first agar in about 15 minutes. Thank you for all the videos!
Very few channels can explain this craft right as a whole. From spore, agar, fruiting conditions and more. Even less can actually teach. Thank you for being one of the rare gems that can explain in a way many can learn. One hell of teacher. A golden standard of one if you will lmao. Mush love ✌
Litterally just put some agar in the pressure cooker and saw this. Perfect timing
Perfect every time
Transferring clean, snow white mycelium onto a plate of virgin agar is one of the things I enjoy most about working with mycelium. Waiting for your LC video now...thanks for helping all of us improve our skills, PGT!
What is the point of that I don’t get it I understand the ones that were contaminated but the clean one why would you want to transfer it?
@@jroc09 To isolate a certain growth.
What would be the benefits of that
@@jroc09 In mycology, there‘s ‚contam(ination)‘, and ‚iso(lation)‘. Isolating a certain growth from a clean culture can provide certain characteristics that you want to isolate, e.g. especially strong growth for fast colonization of substrate.
Instead of throwing out the one with a small spot, do the transfers then put a little h2oo on the spot then cut it out. I have also poured h2oo into the agar up to the the mycelium, wait for the bubbling to stop and pour it off. It pours off the contam with it. Have saved many agar plates like this and grown good mushrooms.
you mean hydrogen peroxide ?
@@SaranshKarira yes
Can u record a video?
what percentage do you use? will 3% do? how about 30%?
@@ankanahumko8486 probably 3%, 30% is crazy strong and would eat through the agar itself
Just inoculated some grain jars, and I just gotta say Teacher....I have always been Interested in the thought of trying to do this myself, and it was YOUR videos that gave me the final push to actually continue and start to teach myself about my new journey in mycology! I'm completely green...and im looking forward to making the many learning mistakes I have in my short future🤣 but I just wanted to thank you for all your efforts, in making all these AWESOME videos that have taught so many like me to just stop worrying and GET INTO IT! I'm trying to not be like so many who rush and make newbie mistakes along the way with dreams of how fast and easy their first flush will be🤣 but we will see how I do...excitedly watching 10 jars of b+ on brown rice grains slowly start to turn fuzzy! I bought spores instead of a liquid culture syringe though...so I've already made my first mistake😂 sorry for the long comment, but I just needed to thank and tell you that YOU'RE the MAN! And NOW? here I am learning about agar from you! absolutely making a difference in the world my man! Mush love
Really
How has you Mycology journey been?
Be prepared to fail. That's not me being horrid.
Im doing transfers off my first agar plates today. Much appreciation for all the info. Mush love. 🍄
When the student is ready the master will appear. 🤟🏽👽❤♾🛸
Love the video and love this comment! I am ready then cause I have found this channel.
@@alltaira5922 same! It’s all about timing ⏱🧫🧬🍄🔥🖤
Yes 💯 !! I'm here I'm ready !!🤩 Synchronization ✨
Not only are rhizomorphic mycelium quicker to colonization of substrate but they are also more likely to create pining/fruits.
Not true homie
Its all about the conditions you have to trigger pinning.
Dont misinform homie
the most elite educational videos here. glad this is free knowledge
Can you please do a video demonstrating how to create/store an agar "library" ? LOVE your content sending peace and love
@PhillyGoldenTeacher I'd like to see this too! 🡹
#metoo
Thirded
I was just wondering about this
Great topic! I am interested as well
Casual flash of the enigma label, been trying for enigma for a while now! Awesome videos!
😁🍄
I did my transfer just this past saturday. Unfortunately this video didnt come out in time for help. But all of them are doing GREAT! nice and healthy and fluffy white myc. Got some Golden Teachers and some Tidal Wave
How do you get those flavors? Could use some spores
Where do you get the spire prints from? Also liquid culture syringes?
@@carinapalomino8069 you cant get a liquid culture syringe but you can get a spore syringe as for where to buy then go to reddit or shroomery.
I'm doing GT right now as well, is your GT mycelium fluffy on your agar like mine is?
@@sheeeitmayn4384 its been a while but yeah i had fluffy growth.
i feel like im watching some professional video on the television. i was so excited for this video!!! long live your channel bro. youre a legend
I used to watch the X files. Then I discovered the Agar files. Thanks so much, PGT 🤓✌🏽
If u put ur cups in ur oven when ur pouring ur agar an putting the lids on in the oven will cut down on contam .350 works great to blows the contam away ,
From mush love ❤️
The Astro way is a great channel too ,
Love ur style great job on ur channel an ur teaching nods like me 👍
If you have left over pennicilin you can extract it and put it into your agar to prevent contamination
I love your videos man, they are always great quality 🎥
Great video PGT. Once an agar cup is fully colonized, how do you store it and for how long is it good for?
Should I put the mycelium up or down against the agar plate?
Just discovered your channel. So relaxing. Thank you for the info and the vibes
You're a god among men for these tutorials thank you so much
Thanks for all the amazing videos you make growing mushrooms very fun and entertaining. I’ll try to grow my very own. Greetings from México
you're an amazing person. thank you
How long can you keep the mycelium on agar for? in storage, like a fridge? can it be used x amount of months later?
U can put plates in the fridge and use them a month later probably even two months but for actual long term storage of mycelium in a fridge you should use culture slants
well looks like i found out what im doing today!
Mush love, keep up the great videos
awesome. I'm trying to figure out how much I need to put into 10oz of broth.
Love watching agar work 👏
@10:00 'Oh-oop" myco talk for - hope that side of the cup was sterile :-) Love your funny and educational videos sensei!
0:19 the top corner growth is not mycelium? Also, once you make the transfer from cup 1 to cup 2, do you discard cup 2 or can use that for grain transfer as well?
Great video. Really have come a long way in the short amount of time you have been on youtube. Really looking forward to the genetics videos.
Question! Do you sterilize your agar solution and then pour into the disposable ramekins? Or do you sterilize the agar and ramekins together like in the pastywhyte tek? I assume the ramekins would melt in a PC but you never know 🤷🏻♂️
I notice that you don't use petri dishes covered with parafilm. Would that disposable container you use still be fine? I feel like air might get inside even when they are closed since they are made of thin plastics.
Thank you for your precious postings
What a great video! I appreciate all of your hard work and making it easy for us!
What's this? A youtuber with affiliated links in the description that actually tells you they make a commission off of them? I'm thrilled! Love your vids.
I'll be doing it soon thanks to this video. The clear closeups were very helpful.
Thanks for the update.
Would storing the agar cups upside down reduce the condensation and also limit contamination?
So glad I found your page mush love
PGT…You da’ man ☮️☮️👌👍❤️
😁🍄
Could you put this leftover in your yard and it possibly grow mushrooms in the wild so to speak
My colonization turned green what did I do wrong ??? will It still pin and fruit or is it a lost cause
If you only have spots what I have done and it worked was take a small piece of paper towel, a little bigger then the spot, soak it with hydrogen peroxide, cover and let sit. I found the next day it was killed and a few days later the mycelium grew over it. If the whole thing is green spray the h2 oo on the whole thing and see what happens. At that point it can’t hurt. Let me know how it goes.
@@kicknadeadcat will do ...Thank you
When making an agar to agar transfer, does it matter whether the transfer is placed agar side up or agar side down?
I am subscribed, how does one get to the discourse community. Outstanding video, PGT. Mush appreciated.
Great vid thanks...
ALSO THANK YOU FOR THE SHIRT! I ♥️IT..
Glad you like it!
Why not immerse everything in alcohol especially the agar cups and lids, then quickly let dry before you pour the agar? I saw the video of working with the cups in front of the open oven on 250 degrees, but I don't think that's enough to sterilize them. Maybe that's why you got contaminated medium?
What is the harm or risks of going from a round one agar straight to grain (assuming a clean looking culture in round one)?
Where does one get sterile agar plates ?
Do i absolutely have to make the transfers? What if i see no contamination?
What do you do with the contaminated mycelium? Can you toss it in a compost pile with a bunch of worms? I would probably do that...
Yes you can compost it 😊👍
I’m sure the worms would love that
Those cups have a recycle code of 1. How do you sterilize them and the agar?
If you have too many successful plates for what you want, what do you do with the rest, fridge? Freezer? And for how long is it good?
Or is it wasted?
Fridge is best for long-term storage. You do not want to freeze! 😁
PGT is my fav shroomer!
PGT- do you sterilize your agar cups? What’s your method?
Is it really speeding up the process by a week or two if it takes a week or two for the mycelium to grow?
Yes, depending on conditions, it can take 6 weeks or longer from a spore syringe to get minor mycelial growth. This method fast-forwards you to that point.
I noc'd spores to grain 31 march, it will be ready to xfer next week. Ill give agar a shot in the next coupla weeks to test a syringe that didnt do shit.
What Are These plastic containers you are using instead of Petri dishes? It seems they also don’t require any parafilm to be sealed. Pretty handy if that works 😉
They are condiment cups for anyone else wondering. They’re pre-sterilized and don’t require parafilm. Also super cheap. Only downside is they don’t look as visually appealing when the mycelium is fully grown out as it does on traditional Petri dishes.
Why do you put the agar pots on an oven shelf??
Hi PGT. Always love your work. Thank you!
Quick one: when transferring a slice of culture from one dish to another, I notice some people put down the small wedge upside down in the second agar dish. Others put down the wedge right side up. Does it matter, and why?
Kind regards, and thanks in advance.
I would be very interested in a liquid culture video.
Just search for Mossy Creek Mushrooms here on YT. They go over LC in detail on their channel.
It's in the works!
All I'm gonna say is stir bars, don't forget the stir bars.
@@AaronAlso in a few months I'll mess with them. For now I just throw in a marble for my LC jars. Stir bar is a luxury for little old broke boi.
@@PhillyGoldenTeacher
Oh! gee, back in my heyday (on "the forums") when I was in college I would have sent you a couple stir bars. You can get a basic kit on Amazon these days for ~$50 with plate, bars, and extractor.
My only problems with LC have been ignoring gas exchange and stir bars; or these days forgetting the stir bars.
I really admire you passing this knowledge on to a new generation of amateur mycologists; my hat's off to you sir.
Top notch as always mate
Do you use anti bacterial agar from the start? Or start with MEA or water based agar first?
Sensei! A Question: After multiple transfers were able to get rid of contam from spores, mycel stops growing after about a week of growth (size of dollar coins roughly). Multiple transfers, same problem. Is it moisture? Heat?
No contam visible, it just...stops.
Can anyone offer advice?
Hey, what do you usually do with the leftover agar with mycelium?
Noticed the PET 1 on the lid of the ketchup cup, which means it can not take high heat. How did you sanitize them?
Alcahol
I really need a new hobby and this is great. Can't wait to grow some lion's mane.
Hi! I just ordered a kit of lion’s mane…I want to take the spores and then eat the mushrooms, I want to know the taste and the texture!!! Then will do the agar, and then the grain n the final touch..long n nice process…one step at the time…good luck to us!!,
I'm a novice, and ive learned alot from this channel. But I don't understand why multiple transfers are needed. Wouldn't that only increase the chance for contamination if the mycelium is clean after the first transfer? Or is it just likely there are contams embedded into the mycelium itself until multiple transfers of clean mycelium are done?
Thanks for the educational content philly!
Love you pgt mush love 🤙🏽🍄❤️
Love your videos man
Would you not sent that last plate as it is?
How do you make your agar in the cups, Do you poor it and put it in the pc, I do that but I get too much moisture in the cups. What is the right way to do this. Very much appreciated for your response. Thanks.
I don’t understand guys what’s the name of that pots ? WhT is the blue stuff?
So you buy aigar powder and a colour and then how you get it to this blue liquid colour?
ALL HAIL THE GOLDEN TEACHER!
ALLLLL HAILLL!
just trying out my first agar plates with some oyster spore . Mycilium is growing like wildfire although ive noticed some yellowing on a couple plates . mycilium piss or contamination ?
Oysters sometimes have yellowish secretions and it is ok
Really need me a flowhood. I wanna try these cups but to worried of contaminants lol. I just stick to no pour tek till I get one
How do you sterilize those mini cups?
That small spot looked like stray mycelium.... they usually happen when there is water dripping onto your spores ill get runoff that starts mycelium on the sides of the cup, but they eventually converge and form rhizomorphic mycelium!
how come you can transfer from contaminated agar and not contaminate the transferred plate but you cannot open a dub tub thats contaminated wouldn't the same rules apply here with the contaminated spores in the air?
How early can I transfer mycelium from a spore swab to agar? I have some specks that are in the infancy of colonizing my agar dish can I transfer the myco that looks promising I have a few strains of Rizo growth but these are small about the thickness of a nickel just little strings the growth on some specks are smaller than a pencil eraser in circumference I’m trying to catch the fast growing myco early by cutting out the spots of rapid growth compared to the rest of the dish before it all combines I guess how early is too early?
Thanks for this PGT
can you use gelatin instead of agar?
How long do I wait to use the scalpel/needle after sterilizing with fire. I don’t want the metal heat to kill the mycelium
What is this metal sterile tray you pur your plates on called & thnks!!
Pgt! I love your channel, you’ve helped me and all of us tremendously thank you! I have a question. I followed your agar recipe, and transferred some agar from another plate just like you did in this video, and almost 2 weeks later I still have no growth in them. I think I might have not boiled the mixture long enough or I might have put too much food coloring. Would either of those be to blame?
Should 5he mycelium be Fury white? I have a plate that I put a small pin 📌stem and cap in and there are 2 round but different types growing
So basically transfer it into a new agar dish and wait for it to colonize that?
3 or 4 times
@@kicknadeadcat so the purpose of the transfer is to produce rhyzomorphic myc and to avoid contaminants growth, right? glad if you could answer m8
@@DEGONDVR
From what I've read in his and other comments, yes it's to promote growth. After the 3rd or 4th transfer you can apparently trust it to rigorously spread
@@sjtv6565 so you have to transfer 3 to 4 times before putting into the grain?
PGT is mycelium a bit fuzzy? It looks great but fuzzy, some cups more than others but I took transfers from a multi spore plate.
tomentose v rhizomorphic mycelium
Im learning here . Is it possible to cut off a bit of mycelium thats just colonised an all in 1 grow bag and put it in a new all in 1 bag to colonised that ?
i cant waint to try
Just wondering, do the lids keep out contamination as well as petri with parafilm?
This is mega informative.
If you made the agar pots and don't have a hood would it be ok to spray some iso alcohol on it to kill bacteria and leave for storage? (With lid on)..
one last thing I'm going to try some clove oil in my agar mix would that work... thanks
If you take a culture from an apparently healthy dish do you throw out the original dish with the healthy culture or keep to take more samples from?
Hey PGT, how do you store your agar dishes if you make them before youre ready use them?
Curious about timing. Total newbie here-sorry if it’s basic... Does a spore to grain take about the same time as a spore to agar in terms of mycelium development and growth? My guess is spore to agar works faster?
are transfers necessary before spawning to bulk?
How long does a agar plate usually take to completely colonize? I inoculated played 2 weeks ago and they seem like they’re barely colonizing. They’re growing in very thin. Is it bad if I just let the agar sit there to see if it’ll colonize more ? I don’t have any contam & I’m just confused at this point. Could it possibly be bad genetics? I’m a amateur btw only 2-3 months into this hobby 😅
Curious why nobody seems to put antibiotics in their agar. That would reduce the bacterial contamination that you're seeing
Bactertial agar is only good in special cases for extremely dirty cultures like wild harvested or special cases. It can create resistance using it often. Generally speaking, it's not something that should be used unless necessary.
That last agar dish that you did the transfers from can you still reuse that