Sporeswabs on Agar - The Agar Files
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- เผยแพร่เมื่อ 30 ก.ย. 2024
- Sporeswabs to Agar
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Phil, listen man. You don't get paid enough for the amount of information you give. But just know, we love you!!!
😁🍄
The soft jazz makes this the most soothing agar video ever.
We’ll played Sir... Well played.
Dude seriously. Plus his voice is so smooth and nice
Jazz?
Jazz?
lol yeah man, well produced... nicely edited and calm nice voice... i feel im on the right path to fixing my mind and life
@@ChadConnor chad the music professor
I like to flip my agar as soon as it cools so condensation doesnt fall down onto the grow media. Especially with spores, you can always store the cups upsude down and even inoculate them upside down, imo this can help prevent anything falling into the agar when you open the cup. Agar to agar isnt so clean, sometimes the agar will fall off, so I let a2a transfers sit for 48 hours so the transfer has time to bond to the agar then I flip it upside down. Honestly, your videos have gotten me inspired to pick up the hobby again! Thank you for getting me off an 8 year hiatus!
Great comment great Info
He’s back!
Favourite time
I believe the pin mold on the last agar was from the wood on the swab.
most likely, the others didn't become contaminated
Or possibly the clippers he snipped the wood with
@@dawncaron3960 that’s what I was thinking
I started 2 tubs 16 days ago, then started the fruiting cycle 4 days ago & I have huge fruits already! It's amazing how fast they grow! I watched all your videos & used your techniques & I have success! Thank you PGT! you're the best!
Hey mate, thanks for the new video, heard youtube's been cracking down on myco youtube so thanks for keeping the flame burning!
What he said !
@enochian seven that is absolutely true
Just discovered your Channel this morning and binge watched all the videos already. Super simple to understand, even for beginners like me. Keep up the great work. Thank your brother.
Another gem! My fav TH-cam channel. I’d love for you to make a vid on how you choose and isolate the mycelium on those plates 🍄❤️🍄
Im surprised he didnt speak of that. Ideally, you want to transfer long before the cup fully grows
2 months ago I received a back to roots grow kit. Today I have agar plates germinating from cloning, a jar of liquid culture from pink oysters and some rye grain spawn as we speak.
Waiting on some spore prints and syringes to arrive now.
All this to say thank you and this community! Still learning so so much!
You needa make full audio books your voice your way of just the right amount of small talk with the amazing 👏 amount of knowledge of just on pont ....you def are def my go to every vid has 5 or more learn something new almsot every time 😅
I was about to waste some money on Petri dishes but those sauce containers are genius! Always learning from the teacher.😂
They work! 😄🙏
@@PhillyGoldenTeacher Hey I have mycelium that began turning grey in some of my jars after being exposed to higher temps. Are the jars sill usable?
You made this for me after I dumped a trillion spores in my dishes haha thank u so much better!!!! Blessings 🍄
As always thanks for the awesome lesson!!! Quick question how long can you store the agar? I’ve watched you all your videos and don’t recall. Thanks and keep them coming! Best lessons out there.
He says it in the easy as 123 how to makes agar vid
Can someone explain why you need to clean the mycelium up by transferring it to another agar cup? If it colonized the initial agar, shouldn't it be free of contamination and ready to be put in grain spawn?
3 generations of transfers is usually the safest practice, so that you are absolutely sure of your sterile tek.
I found some wild beef steak mushrooms, its super rare in my area and I'm hopping to do this with them, once I have some on Agar, are they able to grow in a coir mix? I cant find anyone growing them in a hobby setting.
You can transfer to some wood 🪵
Hello Master Sensei! I have a quick question! Have you ever noticed a bad smell leaking out of your Brown Rice jars smelling like old kitchen waste/sour milk while t1e myc is thriving and looking really healthy? I need help, I just tossed my first ever batch onto the compost pile because I thought it got contaminated and is a goner. The I fo I get through reddit and verious forums are so controversial that I need the grandmasters opinion! Please guys like the comment so Philly will notice my comment! Mush love!!!
I just received a fistful of swabs yesterday, so thank you for the exact class I needed today! 🍄✌️
Started on Agar for the first time a few weeks ago. I've done several transfers. To about the third down the line. How do you know when you have a mono culture or whatever? Is the only way to figure it out to grow it out.
Willy Myco has good videos on making monocultures just make sure you put the video on 1.5x speed lol he talks kinda slow
@@ianlicko5980 I watch him also. Still don't get it all the way to the end. I wish it was more step by step. Like showing the transfers and having them labeled and what the results were and why he chose that piece.
Look up let's grow mushrooms 2 no 7 isolation
@@guystokesable That was made by Roger Rabbit at shroomery years and years ago. I've had several conversations with him. Best dude ever. Taught me a lot. I'm good now. This is a year old. Now I'm a member of TTF. Same one Philly is apart of. Thanks for trying to help though.
@@mattbrady5483 yep, it's also the best video that explains how to isolate a mono culture. His a good guy unless you post something as a fact on the shroomary when you don't know lol.
When you take the spore swab on the mushroom dont you pick up a lot of bacteria along with the spores ? Would uv light help, a little below the the mycelium damaging levels ?
Nice! Was waiting for some more "golden" content. Im all excited, have mycelium finally goin for the first time. Thx to you Golden and funguy. 50 cups only 3 have contam. Did somethin right, thx man.
thanks for the great channel ...how do you store the germinated agar cups (in normal light or dark? does room temp matter) and how long can you keep them? this is fascinating.. thanks
yay, more mushroom sexxxy time! It's just a shame that we can't breed panda bears in captivity like we can our mycelial overlords
Hey man, these vids are great and have inspired me to start my own! I was wondering if you do find some contam in a mason jar should I try and remove it or am I just screw on that jar. I just had to throw out a fully contaminated one with pin mold, I saw it early and just decided to see what would happen (I was lazy and couldn't find if they could be salvaged or not)... the mold was definitely interesting looking haha
if your grain spawn is tamed out toss it, :)
@@antihero105 dang... may move them to a separate location if I spot some in the other jars because pin mold deserves love sometimes as well even if it wasn't what u necessarily wanted
That is the fucking cleanest swab I've seen in my life lmao my swabs are consistently dirty
Of you are using spores from.a print or from a spore syringe they are dirty in and of themselves because mushrooms are fruited in a non sterile emvironment.
As soon as you opened that first ketchup cup i sneezed on my phone keep close eye on that one sorry friend lol
Great video as always. I wish you post some videos discussing contamination, how to prevent them or treat them? How can you save a contaminated batch/cake? etc
NEW VIDEOOO
I made 300 petris and just got done. I bet all of them are gonna get messed up even though my room is as sterilized as its going to get.
how are they looking ?
So are you just pulling these ketchup cups out? And pouring straight into them? Or do you give them like a spray with 70% ISO first and wipe them down?
Did my agar ketchup cups following your instructions. Three days later, my agar cups have little white fuzz balls where I swabbed the spores. I have the cups placed in an incubator around 75-80°f . I used a totally sealed airbox and rubbed all the cups and lids and everything I was in contact with in isopropyl alcohol but I did leave lids off while pouring directly i to cups im hoping I didn't contaminate anything but I have little white fuzzy balls growing off the clubs of spores. I also used a normal q-tip that I dipped in alcohol both sides and let dry in my SAB hopefully the alcohol did short work of any bacterial or mild spores.
Amazing video, took so many screenshits. Been trying to see what myc looks like on agar from spores. So educational, thank you!!! 🙏🙏🙏
forgive the noobie question - i come from the world of growing cannabis.
you said at 2:54 that those are multiple genetics popping off in the agar. so does that mean like cannabis you can essentially cut out the different "genetics" out of there and put them into different agar cups to separate these genetics - grow them out to find the strongest growth / biggest fruits and rinse/repeat to find the best growth? or is this something that isnt usually done. done a lot of cross breeding cannabis in my 20s in the medial marijuana industry (so legally there YT Lol). appreciate these awesome videos with amazing information. thanks so much.
I've been waiting for this
Every time I put a swab to agar, it grows in super super thin... like almost can't even see mycelium.
Why is this and how do I fix it?
Deleted my original comment, I missed the first bit!
My question was answered lol 😂 thank you once again sensei
what will happen if you inoculate your agar with 2 different types of mushrooms? will the stronger mycelium over take the other one or will you the mycelium mix and you get a different type of mushroom?
Thx so much...Where do i store these for incubation? should i use a heating pad? heater? whats your recommendation for the best growth? Is there a video of what to do or buy for the next step?
What’s up with discord? I can’t get on anymore? Went to laptop and change NSFW to except but still no good. Help
big ups brother. Mush love from Downunda Pants oi oi oi oi x0x0x
I started seeing growth 2 days after I swabbed my cup. Is that too soon? Could that be contamination?
5:06 Not all heros wear capes.
Philly Daddy - I'm ready for agar!!! Thanks for keeping the info flowing! 🙋💕
Gonna have to do this with my lions mane spores in the martha tent and cover the base with tin foil next time. The black tray i used is covered in white spores im sure itll be a bitch to clean and the racks are rusting from the fogger. Not sure how long i have until they go bad just sitting there though
@phillygoldenteacher can you use Pete discs if you don’t have coca coir
what i do is i scrape the swab with my scalpel and get a handful of strands with spores on them, then i streak the agar with it.
Do you really need to transfer? Can't you use it directly on grain?
I find leaving that piece of Stick often contaminates the dish. Ha was typing this comment while still watching & sure enough his plate with stick is contaminated 🦠
What would happen if i just cut off the tip of the swab and placed it in a rye bag?
I'd would patron if I get classes or one on one.. Is that a thing still?
Hey where can I find the metal riser you are working on top of?
Can you do a video about sterilizing q-tipes?
You beast I just got some lol
Good morning PGT!!!
If you're not an educator by trade you should be! Good job🤗
If I ever get swabs, I plan on clipping the top in order to get at least 6 pieces to put to agar.
I wish I could share pics on here, but it's one I saw elsewhere. Looks almost like a q-tip umbrella 😂
wish I had Spore for Cubensis or Blue meanies
Is there any application where a spore print is necessary?
Wouldn’t it be simpler to cut of a piece of cotton and put it in the dish?
Those tiny plastic cups survive a PC? Are they a special kind or just the normal ones? They are special kind where do you acquire them?
They are disposable condiment cups, they don't get pressure cooked, they are good to use right out of the package without sterilizing 🙏
Hey @PhillyGoldenTeacher I've been getting green molds in all of the CVG bulk spawns I've done lately, which is about 4 or 5. Im even pressure cooking my substrate now, and I am super careful with my sterile methode, yet still mold. Do you think it could be room contamination (if there even is such a thing)?
Definitely such thing, mold release spores deep clean the room you are doing everything in. Make sure the air is off
Oh there most definitely is a thing and this is prob your problem. Got to clean every surface in that room. Bleach. Rubbing alcohol. Lysol.
Thank you for risking contamination for our education and entertainment
🫡🍄
Best bang for buck as to where I can find swabs
Wish I could just rent a room from this guy and learn everything he knows
😄
Honestly same 😂
Lemme borrow one of those white ketchup cups
Couldn't I just scrape spores straight onto the agar from the spore print? Seems like that would be simpler?
You can, yes, but it's best not to do so much movement over the agar dish and to use a loop or something more suitable for transfering the spores. But it will work! 🙏🍄
Ayo this video is not in the agar files playlist.
Love the neon blue food dye agar....
What size dishes u use do u have to have a flow hood😘
Can someone explain how to make agar?
Can I get sterilized spore swabs from any pharmacy?
They don't always carry them at pharmacies, but yes, if they have them! You may need to look for a medical supply store or order online, they are relatively cheap
Theses are magic shrooms right the u trip
So you have a video showing types of contamination or mold that can be on the mycelium
Jokes that the one that was a fail-safe, failed.
My first MSS to sterilized grain bag attempt resulted in lipstick mold.... Mycelium was about 100% and then I get pink shit. Wtf lol. Now I got a flow hood, agar plates and agar on order along with a few other supplies
soundtrack by kk slider
So I've failed and failed again. Likely the result of way too much info from way too many sources with unneeded information (that and shit suppliers.) Regardless, I just want to say "THANK YOU" for all of these videos. They are short, informative, and to the point. Wish I had found your videos 2 rounds ago. Needless to say, you (and one other) are my mentors for round 3 and forward.
Best video about this on yt
Should we put them in a dark place?
My Spores that I dropped on agar just won't grow any mycellium. I don't know why, I did the agar like in many recipes. After that I decided to buy pre made petridishes just to see if I made a mistake with the agar. Still no growth what so ever, I'm really confused
Is it necessary that you do a couple transfers when using a spore swab or could you go to grain from the first round on agar?
You can go from the first transfer if it is viable enough, but it is ideal and standard practice to do a few transfers to clean up the culture because spores are inherently dirty 🙏
This just made my weekend thank you PGT!
Wonder if the contam was somehow on the stick end of the swap whenit dropped in, Maybe from the scissors that cut it?
Thank you good sir
Could you simply take 5 or 10 cuts of mycelium from the same single agar cup to inoculate multiple grain jars? I hate to waste. Thanks!
Is it possible to use grain from like a grocery store rather then buying a sealed bag online?
Yeah 😄
Sensei, what's a reason to do sporeswabs to agar instead of spores directly dropped into the agar? Thanks for another great class!
He said that using swab on prints can make you get upto 100uses from one print
If that was what you meant if not disregard my comment
@@oNorw Both methods work really well. I’m having decent success with both.
I have a question about when you scrape spore prints in front of a hepa filter and try to get it on agar it usually blows away..... any tricks to make it land on the agar.... ?
*smooth jazz playing in the background* 🎶🎵🎶🎶
😁
@@PhillyGoldenTeacher mushluv
@@PhillyGoldenTeacher Hey dude, is there a list of the music you use somewhere? would love to pop these tunes on my mp3 player. Thanks for all you do.
Hi,
What kind of cups do you use, and where can you get them from.
Are the sterilized or do you have to do that yourself and if so how do you sterilize them.
I didnt pressure cook my q-tips before taking a spore swab from mushroom gills and storing it. Hopefully itll be alright it makes sense to pressure cook them before touching your spore print again and again but idk
Hi. I am new to growin mushrooms and i have a question. I could find only plastic pitri dishes and it does not say sterile. Can i sterilise them with a foil in cooker?
Do you swab the mushrooms post-dehydration, or when the caps open, or? Thanks in advance!
is that wax in the agar dishes?
Silly question teach but where can I order that metal grate and others I’ve seen in your video? I am building my own shroom lab and got most of the things I need and awaiting a few others but I need a few of these different metal stands and grates I’ve seen in your videos. Thanks for the help.
I have anxiety watching this, I can see all of my agars have bacterial contam by direct touching them, especially with the damn condensation.
How do you understand that your spore print is mixed with contaminants? Like if you got contam on the aluminium sheet while taking the print?
How should i store sporeswabs, should i just put them back in the bag, can they last for long time in there?
Gonna have to do this with my lions mane spores in the martha tent and cover the base with tin foil next time. The black tray i used is covered in white spores im sure itll be a bitch to clean and the racks are rusting from the fogger. Not sure how long i have until they go bad just sitting there though
I innoculated with syringes and prints onto agar more than a week agao and nothing is growing on it , the temp here is 15-20 *C, any reason why it may not be growing?
Is pin mold as I’m hearing it dangerous if I came across jars I may have neglected and opened it smelling a musty mold strong smell?