In general pressure feedback means a column issue. If pressure is too high it is often a result of columns with too much residual material build up (older columns) or a mismatched column (pore size too small creating back flow issues). Some solvents can also cause issues, especially if they are viscous and thicker.
I have an intro GC video already but it is oriented more towards undergrad labs and lab report writing. Down the road I can consider a more formal series like this. GC will follow the same general premise but you will be looking at gases carrying analytes instead of liquids.
thank you so much...Please do you know how a column is made???, I mean how to fill it with silica, despite its very small size. Please if you have a pdf put the link here
If factory new and packed not usually. Most columns (especially c18) tend to be fairly stable. However is a column is open its lifetime can vary by many factors and does not guarantee a given number of runs. What you are separating, solvent choices, and proper column maintenance (not leaving water in columns for extended periods) are all important.
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I have a Phd in chemistry and have used both flash chromatography and hplc and still learned a lot from this video thank you!
Thank you for the content, v useful for brushing up. Just a small correction if anyone else is confused. ~ 30:45 mark looks to be a fronting peak
Very informative! It's very helpful for my interview. Thank you sir!
Awesome lecture! Super informative! Are there still plans to continue this series?
I've been waiting for lecture 3... who knows when he'll post it!
It is in the works!
ChemComplete Great! Looking forward to it!
Your lectures are absolutely fantastic! Thank you!
can you please make a video on ''reading chromatograms'' and calculations based on different results.... etc
Thank you for your educational videos!
Could you consider talking about pressure feedback in one of your future videos?
In general pressure feedback means a column issue. If pressure is too high it is often a result of columns with too much residual material build up (older columns) or a mismatched column (pore size too small creating back flow issues). Some solvents can also cause issues, especially if they are viscous and thicker.
youre the best. Helping me so much
Thanks! I’m applying for a job and I don’t have prior experience with xD
Without a study?
Why are the C18/C8/C4 columns the most common alkyl groups? e.g., why aren't C17 or C9 or C5 columns as common?
These are really good, can you do a video series on Gas Chromatography?
I have an intro GC video already but it is oriented more towards undergrad labs and lab report writing. Down the road I can consider a more formal series like this. GC will follow the same general premise but you will be looking at gases carrying analytes instead of liquids.
Kindly upload gradiant HPLC sytem
thank you for these videos. very informative
Please guide us with type of columns in L1 series please
What columns and mobile phases do you use for analyzing berries for well-known pesticides?
Thank you so much!! Very informative lecture!!
Glad it was helpful!
thank you so much...Please do you know how a column is made???, I mean how to fill it with silica, despite its very small size. Please if you have a pdf put the link here
Thank you for the great video! Does the lack of end-capping cause tailing or fronting in the peaks obtained? :)
I think it would cause tailing
Tailing
Thank you very much.please teaching LC-MSMS.
Hi sir,is there any expiration date for Hplc columns?
Thank you sir.
If factory new and packed not usually. Most columns (especially c18) tend to be fairly stable. However is a column is open its lifetime can vary by many factors and does not guarantee a given number of runs. What you are separating, solvent choices, and proper column maintenance (not leaving water in columns for extended periods) are all important.
@@ChemComplete thank you sir.
Some columns for SEC analysis from General Electric do have a Best before date. Specially for columns that can be kept on the fridge.
@@youtube_user9110 thank you.
Thank you
I wish I could give you more likes :)
Thank you so much
Thank you so much, your videos are incredible, greetings from Mexico!
Plzzz upload all HPLC AND GAS CHROMATOGRAPHY lectures from D C HARRIS BOOK
that was a good lecture thank you
Very informative ...ty
Perfect❤
great
Thankuu
Taylor Donna White Helen Jones Thomas