Introduction to HPLC - Lecture 4: Ion Pair Chromatography
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- เผยแพร่เมื่อ 20 ก.ย. 2024
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A lecture series on HPLC covering everything from theory and background to practical trouble shooting. Lecture 4 provides an overview of ion pair HPLC in which ion-pairing reagents are mixed with mobile phases and bound to a reverse phase HPLC column setup for separation and retention time analysis.
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He is just star. He is brilliant and there is no praise for his work. So simple and well explained.
Wow, thank you!
Loved your lecture....Now I am confident with HPLC Thank you so much
I was searching for an explanation about HPLC IP and I am now aware about what's going on inside that column haha. Thank you so much!
I’m waiting for next one
Hi Dr thanks for your time , it was too much excellent explain for IPC
I love your explanation! Thanks ❤
Great video man, very useful information!
Thank you so much for this great lecture!
Great material! Thanks so much.
Thank you sir. I hope you will guide us about some problem in operation HPLC and the trouble shooting (ex: high backpressure; strange peaks,...) How to wash column to assure the analysis result. How to check all the HPLC system daily,....
My boy at it again!!
thanks for the lecture !!!
Greatttttttttttt. Thank you so much
nice lecture series.....
Please do normal phase and make it available to buy!!
Thank you, amaizing
thank you so much! :)
Thanks for your lecture, Sir. I have one question about ion-pairing reagents. I am setting up a method for sinigrin (target compound) detection. In the reference method, authors used TBA (hydrogen sulfate-SO4) reagent but I don't have that chemical. What I have is TBA (hidroxide-OH). I am wondering whether I can use TBA (hidroxide-OH) pH 7.5 adjusted with phosphoric acid to replace TBA (hydrogen sulfate-SO4). I am looking forward to hearing from you soon.
Thank you so much for the lecture! I do have a question. I do know that once you introduce IPA to C18, that column becomes dedicated to IP RP as you mentioned. But can I switch between different alkylamines in order to determine the optimal IPA for my negatively charged analyte (acid)? For instance, maybe I start with triethylamine, do some separations, wash the column with ACN without IPA, then introduce DIEA or hexylamine for my next test. Or do i need to buy separate new C18 columns for each IPA? I only need to use alkylamines as I do not need to analyze positively charged analytes.
May I ask one question? Is this the best choice for separating amine salts (e.g. phenethylamine.HCl)? Or could this maybe achieved with a C8 column with polar end-capping together with a non-polar mobile phase (e.g. hexane + THF)? Might it even work on a C18 column, even though the amine salt will interact with the hydrophobic tales even less?
I guess to take this question even further, are there columns that could separate both freebase amines and their salts, assuming one varies the mobile phase accordingly? (and maybe during sample prep, separate the sample by pull the salts into a polar layer and the freebase amines into a non-polar layer)
Sorry for the rant but for some reason I can't find the answer to this question anywhere...
Release the next sreies plzz
my first year biochemistry prof should be giving me a refund...and an apology
Teaching methods can make a world of difference. I hope you found the lecture helpful!