Cyanmethemoglobin Method Hemoglobin Det'n

Cyanmethemoglobin method is the Reference method for determining Hemoglobin. Hemoglobin carries oxygen from places of high oxygen pressure (lungs) to places of low oxygen pressure (tissues), where it readily releases the oxygen. Hemoglobin also returns CO2 from the tissues to the lungs.

[@cello9877]

This is sooo helpful for effective learning as we still can't be able to perform due to pandemic. But I hope we will soon and can't wait for this to perform. So glad this is Rodak's based too. Thanks for the visualization! I hope you make more videos like this :)
Edit: Grateful to discover this channel. More power!

[@Nora-qu9rq]

thank you so much

[@musamgulila577]

Well understood thanks

[@Saru_0.0]

thank you a lot , very useful video full of information,
but i have a small question..
does the standard mean the drabkin solution?

[@IvyPDC]

Hello Sarah! Thank you for appreciating the video. The Hemoglobin standard mentioned is a commercially prepared standard that is included in a kit for Hemoglobin determination. :) The kit may also include the Drabkin's reagent used for the assay. :)

[@colinwilliams881]

Hi! What are the reference solutions for cyanmethemoglobin po? Thanks :)

[@rominathereselim2850]

Hi! Why would you put only 20 uL? Since 20uL of blood is added to the Drabkins, does that mean it is diluted? ; and the Hb concentration you calculate from this diluted blood is not the actual concentration of the whole blood? Thanks in advance for clarifying.

[@IvyPDC]

Hello there! :) Thanks for watching the video. The amount of blood (20uL) that was used in the experiment was based on the procedure found in Rodak's Hematology which is our Reference book for Hematology. It was mentioned that a 1:251 dilution should be made and that will be achieved by diluting 20uL blood with 5mL reagent. This large dilution, as literature suggests, will help eliminate problems of turbidity caused by lipids, proteins, and other cellular matter that may interfere with spectrophotometric estimation. Another reference book that used 20uL blood and 5mL reagent is Hematology by Barbara Brown. Some Cyanmethemoglobin kits also use this dilution. As for the next concern about the blood being diluted, this is addressed by the determination of a standard Hemoglobin and the computation that involves the concentration of the standard and sample from the spectrophotometer. If the computation will not be used, another method is to use a Hemoglobin Standard curve that involves several dilutions of a commercially available standard. Hope this helps. :)

[@RicardoChinzou]

Is it not that Hb is first oxidised by potassium cyanide to methaemoglobin then by potassium ferricyanide to cyanomethaemoglobin.

[@flavaa9494]

i am so confused