What about distinguishing Segmented vs Band ("baby") neutrophils? A high % of baby cells can warn that you checked while the patient is still rallying more mature cells (mature cells may be too low under normal or deficiency conditions, or are being destroyed somehow). Similarly, when interpreting RDW (Red Cell Width) &/or MCV (cell volume) means or averages, one must be mindful that averages can mask opposing extremes (ex: Macrocytic Iron-deficiency anemia vs folate vs B12 vs Blast (baby) cells, etc). These are powerful arguments for benefits of manual differentials (as are "incidental" observation of spirochetes, extreme mitosis in cancer patients, rare parasite, etc). Thank you for reviewing this lab test which can be performed my anyone with access to a microscope (stains, available for anyone to buy, help, yet aren't essential for experienced observers).
Either ways its fine. Even if you dont use coveslip, you can easily remove the oil from the slide using a cotton dabbed with acetone. That will remive all the oil from the slide without removing the cells from the slide. Ofcourse a bit of the stain from the slide surface (but not from the cells) will come off on the cotton. But that is a minor concern. I do it all the time. Specimens smeared on glass surfaces are incredibly bonded to the glass. So unless you apply harsh techniques such as intentional scrubbing or harsh chemical treatments, then they will be permanently bound to slide
@@TheSingtangpaScienceGuy excellent , thank you for the reply. my microscope doesn't preform oil emersion too well, so I have purchased a dry 60 x objective lens , which preforms reasonably well for analysis .
Leishman's stain. Do watch my video on blood smear preparation and staining. You will learn all about the staining step in detail from that video. Just click on the link given in the description of this video. Thanks.
Leishman's stain. Composition is as follows: 1)Mythylene blue:Stains Nuclei of WBC and basophilic cytoplasmic granules. 2)eosin:Stains cytoplasm and eosinophilic cytoplasmic granules. 3) Acetone free Methyl alcohol:Fixes the smear. 4)Water: as solvent.
The best video for DLC
Thanks, very concise und practical!
Thanks for this helpful video (and many others made by you)!
What about distinguishing Segmented vs Band ("baby") neutrophils? A high % of baby cells can warn that you checked while the patient is still rallying more mature cells (mature cells may be too low under normal or deficiency conditions, or are being destroyed somehow). Similarly, when interpreting RDW (Red Cell Width) &/or MCV (cell volume) means or averages, one must be mindful that averages can mask opposing extremes (ex: Macrocytic Iron-deficiency anemia vs folate vs B12 vs Blast (baby) cells, etc).
These are powerful arguments for benefits of manual differentials (as are "incidental" observation of spirochetes, extreme mitosis in cancer patients, rare parasite, etc). Thank you for reviewing this lab test which can be performed my anyone with access to a microscope (stains, available for anyone to buy, help, yet aren't essential for experienced observers).
Thanks for uploading video....really well explained
Thank you ❤
Thank you sir . Best wishes 🙏
Thank you 🥰
Thank you 😊
Very good making slide
Thank you
good job sir thank you
Thank you sir
Thanks
Thank you.
Thank you...
Super very nice sir
Can anyone please tell me the use of 10x, 40 x,100 x in pathology? Is 10x or 40 x used for scanning?
What are some common abnormalities or deviations observed in a differential leucocyte count, and what do they indicate about a person's health?
Thanks so much
Excellent video!
After preparing the slide, was a slide cover used? Or was oil emersion preformed directly on the sample?
Either ways its fine. Even if you dont use coveslip, you can easily remove the oil from the slide using a cotton dabbed with acetone. That will remive all the oil from the slide without removing the cells from the slide. Ofcourse a bit of the stain from the slide surface (but not from the cells) will come off on the cotton. But that is a minor concern. I do it all the time. Specimens smeared on glass surfaces are incredibly bonded to the glass. So unless you apply harsh techniques such as intentional scrubbing or harsh chemical treatments, then they will be permanently bound to slide
@@TheSingtangpaScienceGuy excellent , thank you for the reply. my microscope doesn't preform oil emersion too well, so I have purchased a dry 60 x objective lens , which preforms reasonably well for analysis .
What's the name of the stain that's used?
Thank you for the presentation 🌺🌺
Leishman's stain. Do watch my video on blood smear preparation and staining. You will learn all about the staining step in detail from that video. Just click on the link given in the description of this video. Thanks.
@@TheSingtangpaScienceGuy thank you so much 💓
❣️❣️❣️❣️
Wow
What what dye solution? Can you use wrights solution
Leishman's stain.
Composition is as follows:
1)Mythylene blue:Stains Nuclei of WBC and basophilic cytoplasmic granules.
2)eosin:Stains cytoplasm and eosinophilic cytoplasmic granules.
3) Acetone free Methyl alcohol:Fixes the smear.
4)Water: as solvent.
Hiii
Hello 👋 where are you from sir 😌
India
Thank you sir
Thank you