fair play to the lady it simply explains the procedure (not sure why albert is present ? ) yet im not sure about the procedure of using a paper towel which is not subject to crosscontamination, very well explained and visually shown ( cant spell demonstrated)
No, there is not a mordant. Heat can be used to drive the stain into the mycolic acid wall. It basically depends on which procedure you are using. If you are using the Ziehl-Neelsen procedure you will use heat and steam it for five minutes. I also have a video for that particular procedure.
@Wressnigg When you heat seal it to the slide, chances are unless you have some endospores or extremethermophiles, you've pretty much killed anything alive on there.
Thank you very kindly; your videos are very helpful! Your students are lucky to have someone so enthusiastic instructing them.
Wow, Thank you!! Very helpful for my Microbiology class. You taught it a million times better than my professor.
Thanks so much! I did this in class, but needed to see it at home again!
-Katie
This was very helpful. you gave so much info and helped me understand.
@jamalshah881 It depends on which procedure you use. Heating it will help the stain penetrate the cell wall.
Thank you. Great Video and explains a lot.
thank you very much! helps with my microbiology class.
please tell me dont we heat it after carbol fuschin and leave it for 5 mins
is it safe to use Mycobacterium in the lab?
PPE is never a waste
"Helpful to me as a student of Clinical Laboratory Science."Thanks a lot...
fair play to the lady it simply explains the procedure (not sure why albert is present ? ) yet im not sure about the procedure of using a paper towel which is not subject to crosscontamination, very well explained and visually shown ( cant spell demonstrated)
so there is no mordant used with the acid fast technique? i thought it was heat.
This teacher is so awesomeee! I love you marry me
No, there is not a mordant. Heat can be used to drive the stain into the mycolic acid wall. It basically depends on which procedure you are using. If you are using the Ziehl-Neelsen procedure you will use heat and steam it for five minutes. I also have a video for that particular procedure.
Hi, How can I get the manual you refer in your videos? i'm really interest on it. Thanks, Daniel.
Like it hope you will keep us upto dated....
what happened if we put methyl alcohol instead acid alcohol?
Don't you have to use steam for five minutes so that the carbolfuchsin can penetrate through the cell wall?
Thanks. good work. pls keep it up
Thank you, thank you. Your very helpful. I like you : )
@Wressnigg When you heat seal it to the slide, chances are unless you have some endospores or extremethermophiles, you've pretty much killed anything alive on there.
Well done
Yes, as long as it is one of the non-pathogenic species.
@SuperJoeOD ...you do realize it's not votile...it's dead...right?
It is impermeable. I just said it wrong at the beginning.
Voice is too low
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