6:07 What does this plot mean? What's our interpretation? As you can see, the absorbance is relatively high for wavelengths below 600, but 600 above is not absorbed and hence, transmitted. So the photons reaching your eyes from going through this solution, mostly have wavelengths between 600 and above and hence, the red color. I hope this helped a bit.
At 2:07 what adjustment does the spectrophotometer makes inside when Transmittance is set to 100% while blanking ? what is behind that 100% transmittance knob?
I am not totally sure of your question here. The sample concentration is irrelevant for this activity. If you plot wavelength on x axis (380, 405, 430 nm....... ) versus the measured absorbance on the y axis you will find the wavelength of maximum absorbance, called lambda max. Each color has its own lambda max. That is the goal for this particular lab.
Hi, this is a very interesting video and easy to understand. I'm currently a second year pharmacy student, and I am learning about Spectrophotometer. Can I use this video for my college project? I'll give BioNetwork some credit or acknowledgement in my video. Thank you so much, stay safe and healthy!
Can you tell me, what is the concentration of your samples, which you inserted into the sample compartment? I'm confused, varying wavelengths when you use?
I was thinking the same thing. If the dilutions are made of the same solution, then why vary the wavelength? That and/or molar absorptivity should be the same, unless again, the samples are different. Also, no need to place the blank every time you run a sample made of the same solution (but only being diluted). Blank should be read again if you are running a different solution sample. Again, that's just me. I think they did these customizations for demonstration purposes.
Hye.. I have a question. If this is for adrenaline test in saliva, how could it help..? the output are all numbers on transmissions and absorbency. the aim to test the adrenaline is to detect stress, and through this test, how could it help? Thank u in advance.
Sir I have a question ⁉️ 1.If you tell the transmitted light in %....then absorbed light must be in %......but you taught absorbed in (nm) and transmitted in % 2..if 10% is transmitted then 90% should be absorbed or used anywhere else?
If you know what kind your unknown substance is like is it a protein or a reducing sugar, then prepare a standard curve of the substance (of known concentration) closest to your unknown substance, and then map it out.
miklaszj non automatic spectrometer in 2018? You've gotta be kidding me, my DIY spectrometer cost $50 and does auto sampling directly to my cellphone screen, seriously, all of that user interaction and sample being completely taken in a different direction each time should make your results have a mount everest of error.
zodd0001 fking yeah, i build an automatic spectrophotometer in my bedroom pretty much the size of a cigarette box that can connect to my cellphone via non standard bitbanged infrared signals using an adaptor in the audio jack to send commands to the spec microcontroller and receive the digital data via the jack microphone input, i spend 50usd in it and the result is pretty much high grade lab quality.
Theoretically you can do the read using an USB sound adaptor and bypass the input cap on the microphone, then use the sound output of a moving loudspeaker with a prism glued on top to filter the light, and a photodiode in the mic input to read the level of absorbance on each wavelenght.
u don't have to set the transmittance to zero before putting in the blank, since the trans/abs will be changed to either 0 or 100 after putting in the blank solution. this extra step is confusing and unnecessary...
Joanna Zhang i made a diy version for 50usd and it can plot the results in the screen of my cellphone, and it's a LOT more precise than this "remove x and exchange it by z" error monstruosity. Good fking luck correlating all of this data points from a moving sample.
very relaxing review before a lab practical
Exactly
Exactly
Exactly
Exactly
Precisely
Excellent video. Clear cut and well filmed.
no cap. this channel goated
One of the best review of Spectrophotometer in lab
6:07
What does this plot mean? What's our interpretation?
As you can see, the absorbance is relatively high for wavelengths below 600, but 600 above is not absorbed and hence, transmitted.
So the photons reaching your eyes from going through this solution, mostly have wavelengths between 600 and above and hence, the red color.
I hope this helped a bit.
It helped a lot! Thank you
Thank u soo much
Thank you for your remarkable conclusion. it meant a lot to me.
Thank you so much for such an effective demo, I got the idea of how to use this machine
best explanation out there on how to use it. Thank you!
Thankyou so much, today is my practical examination, and it was really really useful one😍
very nicely explained and the content is so smooth and crisp :)
have a bio lab practical tomorrow, easily learned how to use it more by watching this video. thanks!!!! :)
could have never found a better explanation than this yohhhh!
You just saved me from failing a bio lab practical! Thank you so much!! :D
@@sasmitvaidya its 8 bro
@@telracsazyl1243 was 6 at the time he commented
At 2:07 what adjustment does the spectrophotometer makes inside when Transmittance is set to 100% while blanking ? what is behind that 100% transmittance knob?
Same question I need an explanation please
Now I have a way to measure if an increase/decrease of algae occurred in a sample of gymnodinium!
Relaxing demonstration with fine video shot
That intro music was fire 🔥
That's awesome! It would help me a lot for my experiment next week :D
I am not totally sure of your question here. The sample concentration is irrelevant for this activity. If you plot wavelength on x axis (380, 405, 430 nm....... ) versus the measured absorbance on the y axis you will find the wavelength of maximum absorbance, called lambda max. Each color has its own lambda max. That is the goal for this particular lab.
Imagine working in a lab full of these retro style equipments 😌
Thank you for upload. The explanation how it works is very good!
bsmls 1b was here
wala tayong ganto sa school scamm
This would come in handy for my lab tomorrow
This video was very helpful to me. Thank you so much for the video
what is the purpose of using blank solution?
Hi, this is a very interesting video and easy to understand. I'm currently a second year pharmacy student, and I am learning about Spectrophotometer. Can I use this video for my college project? I'll give BioNetwork some credit or acknowledgement in my video. Thank you so much, stay safe and healthy!
Yes, you are welcome to use the video as part of your project. Please provide the appropriate reference / citation to this video. Thank you!
Awesome job explaining this, thanks.
nice video helped me to learn more
Awesome and very useful😊😊
What is the blank solution used here?
Why does transmittance of a blank have to be adjusted to 100% before recording the transmittance (or absorbance) of sample at any wavelength?
nice explanation...thanks bio network..
I have a practical too on monday!!!
Can this be used for analysis of phosphorus content in plants
Very good story ❤❤🌹
can you find starch concentration using uv spectrophotometer?
Hey are we increasing the wave length?
Can you tell me, what is the concentration of your samples, which you inserted into the sample compartment? I'm confused, varying wavelengths when you use?
I was thinking the same thing. If the dilutions are made of the same solution, then why vary the wavelength? That and/or molar absorptivity should be the same, unless again, the samples are different. Also, no need to place the blank every time you run a sample made of the same solution (but only being diluted). Blank should be read again if you are running a different solution sample. Again, that's just me. I think they did these customizations for demonstration purposes.
Yes, if your concentration is higher, your absorbance will consequently be higher
How can we calibrate spectrophotometer for lower concentrations of iron? Like in 1-5 PPb.
Hye.. I have a question. If this is for adrenaline test in saliva, how could it help..? the output are all numbers on transmissions and absorbency. the aim to test the adrenaline is to detect stress, and through this test, how could it help? Thank u in advance.
Idk why I find this video so peaceful ahaha
thats awesome!
but i wanna know why are we changing wavelength of light for same samples?
To get a different absorbance values and use it to plot your graph because in each wavelength length the value of absorbance will change.
The soundtrack tho 👌
the video was very very helpful to me.,i request maintenance guide about the
spectrometer video present
it.
THANK YOU SO MUCH
Bery helpul in our chemistry class
Sir I have a question ⁉️
1.If you tell the transmitted light in %....then absorbed light must be in %......but you taught absorbed in (nm) and transmitted in %
2..if 10% is transmitted then 90% should be absorbed or used anywhere else?
someone know why they didn't make transmittance vs wavelengh tab?
Thank you 🎈
Thank you so much
tkanks for demo and practice exp
What will happen if you forget to wipe the cuvette before putting it in the spectrophotometer?
It will show wrong value.
Thank you very much.
why it's adjusted to 100% T every time wavelength is changed?
well explained
Excellent
how to calibrate the spectrophotometer?
Absorbance spectrum for red food colouring
Can you apload another video for polyvinyl alcohol
How we evaluate concentration
Even the man looks like Animated AI robot
❤❤❤
VEry nice bro
Watching in online practical )
Sir I want to check copper purity 99.99%. How to check this process.
Thanks a lot
Very helpful....thanks
hi, why you test the sample again can you reply me
To measure absorbance and transmission at different wavelength
that was beautiful :')
Nice❤
how to analyze an unknown substance using spectrophotometry?
If you know what kind your unknown substance is like is it a protein or a reducing sugar, then prepare a standard curve of the substance (of known concentration) closest to your unknown substance, and then map it out.
@@ishanilahiri7387 hi thank you. i love u so much
lol why do you increse wavelenth by 25 each time
just partice?
It helps alot 👍
What song is this ? Dammmn
purpose of blank?
0:16
you don't this manually in 2019???
so good
❤
What's the purpose of blank here
Fascinating to see. Build one from paper on my channel 😂
I still don’t understand what we would do with these values
1:20 looks like the guy from Jurassic Park
Looks like he knows what he's doing, but his data sheet is kinda sketchy. He's going to run into trouble when he's making the plot...
miklaszj non automatic spectrometer in 2018? You've gotta be kidding me, my DIY spectrometer cost $50 and does auto sampling directly to my cellphone screen, seriously, all of that user interaction and sample being completely taken in a different direction each time should make your results have a mount everest of error.
@@fss1704 What is your phone spectrometer like?
It takes a lot of time make just one complete spectrum. It must be an old version of spectrophotometer or just a didactic one.
zodd0001 fking yeah, i build an automatic spectrophotometer in my bedroom pretty much the size of a cigarette box that can connect to my cellphone via non standard bitbanged infrared signals using an adaptor in the audio jack to send commands to the spec microcontroller and receive the digital data via the jack microphone input, i spend 50usd in it and the result is pretty much high grade lab quality.
Theoretically you can do the read using an USB sound adaptor and bypass the input cap on the microphone, then use the sound output of a moving loudspeaker with a prism glued on top to filter the light, and a photodiode in the mic input to read the level of absorbance on each wavelenght.
6:07 Organic biotags charging for fun
عاش مال تقنيه 😂😂
u don't have to set the transmittance to zero before putting in the blank, since the trans/abs will be changed to either 0 or 100 after putting in the blank solution. this extra step is confusing and unnecessary...
Joanna Zhang i made a diy version for 50usd and it can plot the results in the screen of my cellphone, and it's a LOT more precise than this "remove x and exchange it by z" error monstruosity. Good fking luck correlating all of this data points from a moving sample.
Why did we have to set the wavelength to specifically 380 nm?
استمر حبي
why so serious?
nice..
I almost gonna sleep
Show calculation part
جميل
My god, that machine looks older than me =P
PLEASE SEND ME SAME PROCEDURE FOR WATER FLUORIDE
Ilmunya sangat bermanfaat tapi sayang saya tidak bisa bahasa Inggris
Me too!! Ahhhh :S good luck!!
Dewissengi aga kasi bettuanna
I don't know about that data though...