Thankyou sir for giving all information regarding the colorimeter, now I am totally understood to the how the colorimeter work by two laws, Beer's and labert's law, Thankyou so much again sir👍
Sir i greatly admire your hard work i want to request you kindly make colorimetrically determination of Bilirubin,alkaline phosphatase,uric acid,sgot,sgpt, practical videos. i am in great need plz sir🙏
@@Biochemistrybasics Sir.....a bsc completed student has very low starting salary. So choose MSc . After completion of MSc what jobs are available sir.....?
A complementary filter allows only the color of light that the solution absorbs most to pass through. By eliminating other wavelengths, the colorimeter focuses on the interaction between the light and the colored molecule.
Zeroing a colorimeter before taking a reading is important because it allows for any background color or light to be accounted for and removed from the final measurement. When a colorimeter is zeroed, it essentially establishes a baseline reading of the blank or clear solution, without any sample present. This baseline reading accounts for any color or light that is naturally present in the system, such as ambient light, impurities in the reagents, or any residual color from a previous sample. By zeroing the colorimeter, these potential sources of error are accounted for and eliminated from the final measurement. Once the colorimeter is zeroed, the sample can then be added and its absorbance or transmittance can be accurately measured. This ensures that the final reading is a true representation of the color or concentration of the sample, without any interference from background color or light.
Sir good afternoon.sir I have doubt. That I am using Beacon brand colorimeter for biochemistry test.doubt is that.my colorimeter OD range is 2.00 to -0.31. I 1st gave blank and set zero,after remove blank the OD is 1.69.is it correct working my colorimeter.
Thank you sir..but I have another question regarding preparation of Working reagent especially in urea determination by berthelot method.Can you please explain
I need only a small quantity preparation of working reagent to per patient test.From the kit insert,Working reagent must be prepared by adding all the enzyme reagent bottle(3ml) to Buffer reagent(60ml).and it is stable for 16 weeks.My testing load is very low.I need to prepare working reagent per patient.But I don't know the proportion of mixing.
![CAMPปลิ้น | EP.83[1/2] เปิดแคมป์หลอนต้อนรับ 2 ตัวพ่อแห่งวงการผี!](http://i.ytimg.com/vi/2uPuHYHgwiI/mqdefault.jpg)
Thankyou sir for giving all information regarding the colorimeter, now I am totally understood to the how the colorimeter work by two laws, Beer's and labert's law, Thankyou so much again sir👍
Glad to hear that. Please spare a few minutes and do spread the word about the channel among your batchmates and friends
Sure sir.
I admire your persistence and will to share Knowledge. Keep it up. Best wishes..😇
Sir, you are awesome.You explained every thing in very simple manner.
Thanks dear. Please spare a few minutes and do spread the word about the channel among your batchmates and friends
Thank you sir for such a helpful video🙏🙏
My pleasure dear
Thank you so much sir🙏
Most welcome dear
Sir, why is it important that the marking on the covet should coincide with marking on the colorimeter?
Sir i greatly admire your hard work i want to request you kindly make colorimetrically determination of Bilirubin,alkaline phosphatase,uric acid,sgot,sgpt, practical videos. i am in great need plz sir🙏
Sure. Will try my best
Egg,milk, analysis of blood , analysis of bile,HBA1c
Sir ji yeh practical bhi upload kijiye
Thanks a lot for this video!☆
Most welcome
Impressive sir 👌
🙏😊
Thank you sir for you clear explanation and demonstration
Most welcome dear
Sir BSC nutrition and dietetics or bsc radiology which is best course
BSc radio
In bsc or MSc in radiology what to do next sir ....? Whether we open own daiognastic clinic or PhD which is better in future ??
@@hazel8537 you can't open your own diagnostic center. Though you can work in it
@@Biochemistrybasics Sir.....a bsc completed student has very low starting salary. So choose MSc . After completion of MSc what jobs are available sir.....?
Why we are using cuvett made up of glass in colorimeter and quartz in spectrophotometer
Superb explanation sir..
Thanks ranjan
Helpful video
Thanks dear 🙏☺️
Superb sir , nicely explained
Thanks nazmin
Sir,
Your descriptions is so so nice and helpful to understand clearly. Can you make a video of Blood glucose meter please ?
Md Ali Reza
Thanks dear for the appreciation. Sure will do it
Why different colour filter is used as complementary?
A complementary filter allows only the color of light that the solution absorbs most to pass through. By eliminating other wavelengths, the colorimeter focuses on the interaction between the light and the colored molecule.
Sir why we set the reading at zero after inserting the cuvette?
Zeroing a colorimeter before taking a reading is important because it allows for any background color or light to be accounted for and removed from the final measurement.
When a colorimeter is zeroed, it essentially establishes a baseline reading of the blank or clear solution, without any sample present. This baseline reading accounts for any color or light that is naturally present in the system, such as ambient light, impurities in the reagents, or any residual color from a previous sample. By zeroing the colorimeter, these potential sources of error are accounted for and eliminated from the final measurement.
Once the colorimeter is zeroed, the sample can then be added and its absorbance or transmittance can be accurately measured. This ensures that the final reading is a true representation of the color or concentration of the sample, without any interference from background color or light.
Sir good afternoon.sir I have doubt. That I am using Beacon brand colorimeter for biochemistry test.doubt is that.my colorimeter OD range is 2.00 to -0.31. I 1st gave blank and set zero,after remove blank the OD is 1.69.is it correct working my colorimeter.
1st you have to set the zero and then you have to measure the od of blank
plz make vdeo on Ion selective electrode
Okay. Sure
Tqsm sir, it is very useful to us
My pleasure harshitha. Please spare a few minutes and do spread the word about the channel among your batchmates and friends
Sir plz batadijiye kinetic test measurement konsa laboratory machine mea difficult hota hea
In colorimeter it's measurement is difficult. Better do it in semi automated biochemistry analyzer
@@Biochemistrybasics thanks alot sir 🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏
Great sir
😇
Thanks a lot sir...😊
Thank you so much sir nice video 🥰🥰
Thanks सकीना.
@@Biochemistrybasics you are welcome 😇sir
Sir kya colorimeter me sare biochemistry test perform kiye ja skte hai Erba reagent ke sath.
Yes. Almost all except tests which require multiple calibration
@@Biochemistrybasics thanks sir ji
Sir pls mention zero settings through technical engineer
Okay
Your all video are helpful for us
Glad to hear that jayshree
Thank you sir it helped me in university exam
Wow. Glad to hear that
Very nice sir👍 sir can I add beer Lambert law in spectrophotometry in slides....?
Yes you can add
@@Biochemistrybasics okay sir👍
Thank you sir
My pleasure bhagyashree. Please spare a few minutes and do spread the word about the channel among your batchmates and friends
good explanation thank you
Thanks Upendra. Keep loving biochemistry and do spread the word about the channel wholeheartedly
@@Biochemistrybasics sure sir thanks for response😄😁😊
Thank you so much sir❤️
My pleasure संस्कृति. Keep loving biochemistry and do spread the word about the channel 😊
Sir thoda Hindi me bhi bta dijiye taki hme bhi kuch samjh me aa jaye please sir .
I request you sir 🙏🙏
Sir please post video on principles of estimation of urea by different methods and standardisation and estimation of urea by dam method
Yes syeda will do. Video on estimation of glucose by GOD POD is there. You can watch it.
Sir please post video on sgpt
Sure. Will
Thanku sir
Your video help in our practical viva🙏
@@docactor3637 glad to hear that. Please spare a few minutes and do spread the word about the channel among your batchmates and friends
Ofcourse sir
Digital Colorimeter me test Kaise karte h sir Jee test karke procedure blood sugar Ka test
Take the OD of blank standard and test and enter the value in formula and calculate the total amount of sugar present in the solution
I habe monozyme innova 1000 colorimeter. 520 filter me zero settings thikk se nhi ho rahahai
Please do check with biomedical engineer
thank you sir!!!
Most welcome
Can I use Euro Diagnostic Biochemical Reagents in Colorimeter
Yes you can
But read kit insert before using it
Thank you sir..but I have another question regarding preparation of Working reagent especially in urea determination by berthelot method.Can you please explain
@@phoenixmt8657 what's the confusion
I need only a small quantity preparation of working reagent to per patient test.From the kit insert,Working reagent must be prepared by adding all the enzyme reagent bottle(3ml) to Buffer reagent(60ml).and it is stable for 16 weeks.My testing load is very low.I need to prepare working reagent per patient.But I don't know the proportion of mixing.
How much blank we keep in the tube....
One ml
What is mobile phase and stationary phase in colorimrtry
There is no such phase in colorimeter. It comes in chromatography
Is se aagy b kar K dikhao kesy perform krna Kon konsa test perform kar skty
@@UsamaNasir-q1g Alag se sare video Dale hue hai
Thanks sir❤️
❤️😊
Thanks
Sir ap Adani gaims.me padha te ho
Yes.
Unknown compound p bhi bnaye
Okay
Thankyou so much sir ji
❤️❤️
❤️
Thank you
I have a practical exam tomorrow and this video contained exactly what I needed
@@ridwansalifu6987 Most welcome dear. All the very best for the exam
TQ U SIR
My pleasure. Keep loving biochemistry and do spread the word about the channel
Thinku sir
Most welcome dear
B S T FULL FORM?
VOICE IS NOT SO CLEAR
plz work on audio quality
Noted 😇
Thankyou so much sir ji
😇🙏
Sir pls aap procedure bta dijiye
It's explain at the end of the video
More videos upload sir ji
Please see "practical" playlist.... All video are there
In Kutch
Hii
Hi
woh lab coat mat peheno sir warna aapke subscriber bhi gir jayenge
Thank you so much sir
Most welcome dear
Thank you so much sir..
My pleasure sreevidya. Please spare a few minutes and do spread the word about the channel among your batchmates and friends
Thank you sir
Most welcome dear