How To Grow Unlimited Mushrooms With Liquid Culture!
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- เผยแพร่เมื่อ 28 ก.ย. 2024
- UPDATED VIDEO HERE:
• Propagate any Mushroom...
In this video i demonstrate how to make a liquid culture of any mushroom species using Karo light corn syrup. This method is bare bones intro to liquid culture. Showing the most basic entry to grow mushrooms.
EDIT:
Use PAPER micropore tape, not plastic micro pore tape. You may use a steam canner (2 hours) to sterilize your culture media instead of a Pressure cooker. using a pressure cooker sometimes will caramelize the sugars (bad) due to the high temp.
In this video i demonstrate how to make a liquid culture of any mushroom species.
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Red rtv high temp silicone- Lowe’s/homedepot/any car place. Use this instead of the tape and it’s a self healing injector port
It's a beautiful thing to be honest
But doesn't this eliminate air exchange? I thought that was the point of using micropore tape
@@DAAI741 drill a separate hole for either poly fill (have to be careful not to get that wet) or look up hydrophobic syringe filters and google that with mushroom and you should find the size you need and videos on how to drill and attach to canning jars
I admire your patience, the amount of already answered questions (particularly the marble confusion 😆) is staggering. Thank you for the information.
😂 Thank you for watching!
Don't mind me....Just gonna binge watch your channel while i keep prepping more seed spawn.
Funny I realize I actually bought my initial spore syringes from this channel and didn’t even realize until now. They’ve worked great!
Thanks for the support! 🍄 🙏
@@SporenSprout after injecting the jars where do you put them to grow and what temp ?
@@strictlyundergroundhiphop5129 on a shelf in a room that is around 75 F
@@SporenSprout in the dark ?
@@strictlyundergroundhiphop5129 yes
Out of all the videos I've watched on liquid cultures this answered all my questions and was the simplest to follow, thank you! 🍄🌱
No problem! Thanks for watching, Check out this updated video!
th-cam.com/video/JYl_UnYK_kU/w-d-xo.html
Wow.... this is a very good idea to have a plethora of any type of species on hand at any given time. Thanks for the knowledge.
Best tat ever!!
🤔
Hello, I love your straight forward teaching style. Thank you. Question: could I follow your spore print method and then directly inoculate jars as you have in the above video?
Thanks! Yes that would work perfect
Its best to use liquid culture as spores take up to a week or so to turn into mycelium and spores are also usually contaminated so its best to do a brf tek with the spores, get agar, take a piece of clean mycelium from either the middle of a cake or the middle of a mushroom or something and place it into the agar, once it turns to mycelium you can use that to create your own liquid culture, you have a much greater risk of contam and contam is very hard to see usually in liquid
Thanks alot man. I rarely comment but I feel you did a great job. No bullshit just information necessary. Do you feel the stirrer was worth the extra cash
Yes! Best thing iv ever bought for this hobby! I think it was like $30 on amazon. As others have commented the stirrer actually brings fresh oxygen into the mixture and speeds healthy growth!
@@SporenSprout great ! Thanks for the info.! I will get one since I'm about to give this a shot.
@@bradpotetz9844 sounds good! If you need any help lmk!
Baby bottle tek was a great easy 1. The vid got taken down long time ago... up t use what you want. I like organic honey. I prefer it over karo.. helps not to use too much!
Great work. Very sterile and described each step very easily to understand🤟
I agree with the comment below. Great, straight forward style of teaching. Thank you for posting this
I appreciate that, thanks for watching!
Q1. Can sugar syrup made out of crystallized sugar be used instead of corn syrup?
Q2. What can be be done if i don't have a magnetic stir? What can be done to instead of that process?
Love 💕 from India
Yes you can use any sugar source you have available. You can just put a glass marble inside the jar instead of magnet.
@@SporenSprout put marble and shake the jar to stir it?
@@nitinroy1331 don’t shake aggressive but yes
Super helpful. Thanks for you r info! Could maybe make an injection port with high temperature gasket silicone too!
Yeah this is the bare bones way to do it.
Here’s the updated video !
th-cam.com/video/JYl_UnYK_kU/w-d-xo.html
I love cheap DIY mycology! 🍄❤️
I saw a guy make a hole, then bead liquid high temp gasket goo over the hole to make a self sealing inoculation point. This could use that to improve it even more
Yes I made an updated video here th-cam.com/video/FZPObmBQ3Ko/w-d-xo.html
Thank u this knowledge i will be using it
Thanks for watching!
This infinite food glitch is wild
Awesome info man!!! I know you said these are for LC syringes, is it the same procedure for spore syringes? Or do the spores need to germinate first? Thanks!
Same procedure. The spores will germinate inside the culture media and become LC
If your talking about making your own spore syringe then no it's not the same. You will need a spore print, and you would not add the sugar to the water. There's videos here on TH-cam that you can follow.
@JonnyQuest [*Newb here*] what does the "LC" mean? (Trying to learn all I need to know and not just listening to one person so I have more of a non-biased, non-judgemental amount of info thats swayed either way)
I appreciate your response in advance😉
@@99thmonkeyresonance66 I think it means live culture? I’m not entirely sure myself also new
@@kamadavana5821 yes it does. Thanks for answering me. There won't be TOO to much here on "EwwTweb", about "growing mushrooms" in DIFF/ALT ways- (mainly- bc 'they', NEED you- to "buy from" THEM. Same goes w/ the "Gardening"/"plant industry's". Tbh, its DE-spicable- IMO.)
You REALLY, FACTually, DO NOT-
"NEED to buy, all the 'special stuff'- affiliated w/their 'operations'."
There are MANY, many diff ways to go about "growing your OWN mushrooms"- whether for medicinal/food/fun purposes.
A good place to start is the Reddit group! "r/mycology"- I believe its called.
Hope this helped.
Am I safe in assuming that, once your jar is colonized, you can then use THAT jar culture to make more jars of culture for a theoretically unlimited supply of liquid culture out of a single $20(ish) dollar syringe?
Yes that’s basically how it works
You should've put aluminum foil ofer the jars in the pressure cooker
You are correct
Yeah.......duh. Everybody knows that.
INFECTED PUDDLE stfu, not everyone does...if you don't have anything positive or helpful to say why say it?
So when you extract the liquid culture into a syringe do you have to add another layer of microspore tape ?
@@user-xu5vn3tp3i Yeah. Check out my newer videos I use better lids
I'm from India
This vdo is very useful
Thanks
If I don’t have a pressure cooker, am I able to boil the jars in a large pot for 90 minutes at medium to low heat?
I would do that for 2 hours and even then I wouldn’t completely trust it. Test the end product on agar before using it.
is there any specific protocol or details for using/transferring/storing the new liquid cultures? also how long does it take after inoculation for it to be usable?
You can watch my other videos for information about how to use the liquid cultures. A syringe is used to extract the liquid culture after 2-4 weeks of growing in the liquid. Can be stored for years at room temp or refrigeration
@@SporenSproutsince commenting i’ve watched a lot more of your videos and they’re great. you’ve done us all a great service so thank you. that’s surprising they can be stored for so long even at room temp!
I'm your 700th subscriber!
Alternative to pressure cooker?
If I do not have a pressure cooker
Steam bath for 2.5 hours
What kind of micropore tape do you use because the one I used melted and came off during sterilization
It’s 3M paper/fabric micropore tape.
amzn.to/3Krhenj
@@SporenSprout thank you!!
Thanks for making this video. How long can such liquid culture last for? Can you store it in the closet or long does in the fridge?
At room temperature they will last up to 6 months. Store in refrigerator for 1-2 years. Thanks for watching and check out the updated version of this video here th-cam.com/video/FZPObmBQ3Ko/w-d-xo.html
What if I wanted to do this but with agar plate instead of liquid?
Yeah that works
After watching a few more times this might seem like a dumb question, but should the lid be fully closed when pressure cooking, or slightly opened.
The lid band is loosened a 1/4 turn. Just to help reduce pressure on the tape.
thank you soo much, youre the man!@@SporenSprout
What do you do with the jars now? Fridge or leave em out?
Leave em out
So with this once you on your last syringe could you just repeat this process all over again?
Yes
So why do i need an agar plate?
I could simply buy a LC syringe from the lab and make unlimitided amounts of LC. And if my LC contaminates i could rebuy a new syringe for 15$
@@spicypunch6029 Yeah that’s correct
@@SporenSprout so i could skip agar plates. But If i mess up and all My LC contaminats i have to Rebuy a syringe.
Probably very frustrating when all you jars are contamineted bc your LC is not Clean:/
@@spicypunch6029 Yes it’s best to make a couple agar plates as backups and for testing
I have a wild idea and I'm looking for some mushroom fanatics to tell me if (and how) it can be done. I really want to have lots of fly agaric in my garden. I'm looking for a way to make this happen. Should I try it with the method of this video? And what kinds of substrate should I use? 🍄 they are soooo gorgeous and magical! Thanks a lot fellow mushroom lovers 🍄🍄🍄🍄🍄
Amanita muscaria forms symbiotic relationships with many trees, including pine, oak, spruce, fir, birch, and cedar. You would have to plant the mycelium in a forest not in a garden.
@@SporenSprout thank youu!!!
Quick question and I'm sorry if this has been asked: could I use a simple (sugar) syrup instead? I don't have corn syrup on hand but I do have sugar, water, and a pot. So I figure I'll ask!
Honey. Pure honey
Yes just use honey
Sugar will work
@@SporenSprout brother, do i substitute honey and corn syrup in a 1 to 1 ratio ?
@@crazygandalfwithweed1180 Yes
I have had two starter kits for over four months and they both would not sprout. They are both very well covered with mycelium
After that long I would assume they are dried out. You should submerge the blocks in water over night and then set the hydrated blocks inside a shotgun fruiting chamber or mushroom grow tent to fruit.
If I just 1cc of culture instead of 2 does that mean I just have to mix it for longer
Yes.
I have a question, I have some dry mashroms from Europe how can I get them to sprout ?
Doesn’t work with dry ones. Find fresh mushrooms. Watch my other videos and you will understand more
@@SporenSprout Thank you .
Do I store it in the dark and at what temperature is best?
Is the Karo Light Corn Syrup has Vanilla? It seems all the Light Corn Syrup has vanilla added
No, I avoid vanilla. Use 1/2 tablespoon of honey into 600 ml of hot water.
How much typically, I know many factors are involved, but with a 10cc syringe, how much shrooms could one grow? Or yield?
Using 2 ml per inoculation, you can inoculate 5 3 LB bags of grain which you can grow around 1 pound of dry mushrooms from. You can attempt “grain to grain transfers” to spread the mycelium to even more bags of grain and exponentially increase output.
Subbed 😤 love the info
Appreciate it brother!
Can it be done with spors
Yes
@@SporenSprout thanks 😊 🙏
Is it easier to do this or just use your liquid culture to do grain to grain transfers over and over?.
This is easier
@@SporenSprout Thanks, how long can the jars keep once they are made?. I assume refrigeration increases longevity?
@@yakivpopavich around 12 months or longer in refrigeration.
When I did this, rust formed on the inside of the lid and dripped down into my jar.
Rust is not harmful to the mycelium but It is very ugly, you can switch to plastic lids instead of metal.
How should it be stored? Light, temperature etc...
If stored, how long can ut be stored before it dies?
Brilliant! Thanks,
I'm trying to grom magic mushrooms what am I suppose to Inject my bag with exactly?
You are supposed to use one drop of your spore syringe on to a agar plate and the mycelium will germinate. Take a wedge of the germinated mycelium on agar and put it to sterilized grain or liquid culture like i made in this video. Or you can take a chance and directly inject your bag with 1 ml of your spore syringe but it might not work because spores are not 100% clean.
i used spores most of the time with 99% success rate. Just keep your area clean using 70% isopropyl alcohol and an air purifier nearby.
Probably 1-2cc is recommended, anything higher will be to wet on the grains and probably will produce mold.
Hmm, how would I go about taking a syringe from my Liquid Culture to make a new jar of Liquid culture?
Use a sterile syringe and tilt the jar and suck up the liquid.
Hi my friend I was just watching you on TH-cam and I will say I was very impressed by your knowledge and willing to share with us. My son has been growing mushrooms for me to eat because of the research he has been doing about the health benefits of a few different types.... I have a question. We are in the process of making the jar myzilyume. We are in Utah so it's extremely cold. For two to three months his been trying to get some to grow. My question is how long will the myzilyume last in the jar before it's not good to grow anything. We want to be ready for when the weather changes and it's warm enough to grow them. We were trying for a long time and finally I told him to go ahead and keep the room really warm and let's see what happens. Two weeks later we were enjoying some kind mushrooms lol thank you
Thanks for the kind words! 🙏 mycelium In liquid culture stored at room temp will last around 6 months to a year and if you refrigerate the liquid culture it will last longer than a year.
I wish you could of showed us the accumulation and collecting process?!
I have plenty of other videos showing the process
Mind directing me to one in particular? With a title?
@@josephrikers1111 Try this one:
th-cam.com/video/EARNzNHAzf0/w-d-xo.html
This one:
th-cam.com/video/JYl_UnYK_kU/w-d-xo.html
This one is good too:
th-cam.com/video/MAcTxk085oM/w-d-xo.html
Great video! I don't have a pressure cooker or a magnetic stirrer. I know they're widely used in mycology but I was wondering if there's any way to sterilize and stir without investing in those items? For example a make shift steamer using everyday household pans (instead of the pressure cooker). And simply physically shaking the jars (instead of using the magnetic stirrer). Any broke boi possibilities there?
You can steam the jars for 2 hours in a pot of water with a lid. You cannot shake the jars if you use a micropore filter because the liquid will touch the filter or even leak from it. You can use a marble and swish it around the bottom to help break up the mycelium instead of shaking or using a magnetic bar.
I have 2 larger white fuzzy jellyfish floating on top I stirred 2 days before and now they appeared, it’s been 13 days sense I injected with LC and mycelium growth is at 400ml when settled very new first time trying jar and I can’t really find an answer if it’s good or bad? Or contaminated? Thank you
The mycelium will start growing on the surface like that when it has used up all nutrients
Thank you
Let’s say I use up all the spores in my syringe. Can I use the newly created spores in the mason jar and suck them up into the syringe and start the whole process over?
Yes exactly. The spores will germinate into mycelium and the mycelium will grow and multiply.
Thanks!!
how long do they last once you make the syringe??
I've got a small cloud forming in jar....now to keep it going, how warm should the room be for it to thrive? It's cold up here in Oregon, should I get a heat pad?
Nice!! You should stir the cloud every few days so that it breaks apart into tiny pieces. I use a space heater in my room and set it at 70-75 F
Very good guy!!
No soy peptone? Is Peptone necessary?
No, just use honey.
Can we make unlimited spore syringes forever? There wont be a problem if i keep duplicating the syringes for generation after generation? Thank you!
Thanks for sharing this vid; very helpful!
Richard File-Muriel no problem! I will have a lot more videos coming soon!
Can I also use a electric pressure cooker for this
Now I can also inject spores into this right to make liquid culture?
Yeah but you will have many different genetics
Hello i have a question. Would you inoculate the substrate with the syringe? If so will each bag that is inoculated with the same syringe be a identical. Thanks
You would inoculate grain spawn with the syringe and once the grain spawn is colonized, mix with substrate. Yes each bag will be identical. I have other videos that show the whole process.
@@SporenSprout I actually just found my answer in another one of your videos lol. Wife and I are just getting into mushroom growing so will be being watching all your videos. Thanks for all your hard work producing these informative videos. Top notch.
@@michaelflores9796 Thank you for the kind words! Growing mushrooms is the most rewarding feeling. It feels great to help other people find the same happiness I did. If you ever have questions you can reach me directly on sporensprout.com
Have a good night!
Really great viedo, very nice and clear.
If I dont have one of these magnetic stirrers can I stir it up by swivelling the jar around with the marble inside?
Yes that will work
I could.not find marbles. Are stones ok to use?
Thank you for your response!
i love this vid this is so understable, thank you for doing stuff like this and i have one question - can i do this also with copelandia cyanescens culture??
Thanks for watching! And yes you can !
Ohhh you sell your own syringes. That’s cool. Ill be ordering some syringes then from ur Etsy
Sounds good! I have a wide variety of species available right now.
Good Vid, clear precise informative presentation. Very helpful.
How do you recommend storing the new LC? Thanks
Thanks for sharing.
How can the mycélium breath and grow in a liquid substrate while it hardly grows on solid substrate with too much water ? Thank you
That’s a good question
I wonder the same thing about how plants can be propagated in pure water and produce nice healthy roots, and then that same plant in soil with too much water gets soggy and the roots rot 🙄
Hi there, thanks for the video. How many generations of LC can you use before it goes bad? Like you apply LC syringe into LC jars, then you use a bit of the solution from each LC jar to make another LC jar. Usually how many times can you do this before we have to start again?
How do you even start again?
Hey brother thanks again for the content. I have an extra LC jar of Cordyceps but I’m not ready to use it right now. I’ve been told cordy LC doesn’t store well but I was hoping to hear your thoughts on how I can make this LC last a few more weeks.
The best thing to do is keep it in a cold room. It will be ok there for 1-2 months.
@@SporenSprout thanks!
Can you use honey if ya don't have the Kayro? How much if so :)
Yes honey is better. I use 1 Tablespoon per 500-600 ml of water
Cool, think an insta pot could work for this too if ya cook for about 4 hours@@SporenSprout
Those syringes that you started with are spore syringes right? I’m wanting to make some lc with several spore syringes that I purchased. I’m just getting confused when you call them “liquid culture syringe”.
No I used a liquid culture syringe. Liquid culture is basically germinated spores. It is mycelium.
You can use a spore syringe the same way as you would a liquid culture syringe but it is considered multispore inoculation and will result in many genetic differences.
No one needs anything but light malt. Mine do just fine and all I use is light malt and nothig else.
So is this to make more syringes to grow more shrooms or this will grow the Shrooms? Sorry trying to learn, and how many ML is 2cc?
This will grow more syringes so that you can grow more mushrooms
2 ml
Can you add spores to the solution instead of LC? thanks!
Yes.
Yes
You should try making those with blue juice instead of water 🧬😈💻👽
Great video man! Is it possible to use honey instead of corn syrup? Like you do with agar recipes?
Thanks for watching! Yeah I use honey for all my liquid cultures currently.
@@SporenSprout thank you for responding so quick brother. I’m going to try this tomorrow, this changes the game for me. Mush appreciated
@@thegreenroomorganic No problem! Good luck with all of your experiments! 🍄 🧫
Thank for the knowledge ⚡️😎
Hi I want to buy from your store but it does not ship to my location. Do you have international shipping?
I don’t ship internationally because sometimes the packages would be refused by the foreign government and I am left to refund.
@@SporenSprout Would you happen to ship to Singapore/have previous customers from Singapore?
@@elizabethtseng1658 I can’t remember. I can take a special order for you but it must be more than just a couple syringes to be worth my time and also I will not be responsible for refunds. You can email me your full and complete address to sporensprout@gmail.com and I will see how much shipping costs.
I'm new to this.. so this ain't the same thing as what's in a "spore" syringe?
Liquid culture is just spores that are introduced to sterile nutrient media causing them to germinate into mycelium.
are these magic mushrooms or just normal mushrooms?
Just normal but it works the same for magic!
Awesome Subscribed !!!
Thanks for watching! 🙏
I appreciate if you answer my questions?
1. So after you do this how long do you need to let them sit and in what conditions until you can draw up the culture into new syringes?
2. How long can you keep the culture syringes and do they need to be in the fridge?
3. In order to grow fruit, will you then inject the same way you'd use spore syringes?
1. 1-2 weeks growth in dark 75-80 degrees.
2. 6 months room temp, much longer if refrigerated.
3.Yes, same as spores
@@SporenSprout Thank you
Great content😍
Thanks brother!
What do you do with syringes after you put the 2cc’s in? Can you refrigerate the syringe? And where do you store the jars?
Yes I save the syringes in the refrigerator. Jars are stored in room temp on a table
@@SporenSprout is there a lifespan for jars left at room temperature? and syringes left in the fridge please? I've just grown my first jar of LC and not ready to use just yet, not sure what to do with it now?
@@wonderfullwombman7352 LC will last many months in room temperature. And many more months if stored in refrigerator.
Loved the vid
I'm i'm a 3world country atm and corn syrup is near impossible to get by. Could I replace it with honey, molasses, etc?
Thanks
Yes honey is the best thing to use.
Can this be done without a pressure cooker?
Yeah you can use a canning pot with a lid and steam bath the jars for 2 hours.
@@SporenSprout thank you, newbie questions but by "marble" you mean any kind of small round sphere like spray cans have inside them? And do i have to shake the lc syringe before use?
Is the magnetic stirrer necessary, or is hard shaking ok? I didn't know if it somehow actually makes that much of a difference.
Yeah the mycelium can get clogged in the syringe if you don’t break it up good enough
Hi how should I store liquid culture? Does it need light and high temperature?
75-80 degrees while it’s growing and light does not matter.
How long does the liquid medium last before inoculation?, I got some of my cultures in but currently waiting on more but would like to get all these ready for when they arrive.
Months. It’s actually good to let it sit for a few days before inoculation just to make sure no other growth appears. Btw you should use 1 Tablespoon of honey instead of karo. It’s much better.
@@SporenSprout Thanks I'm using honey but after pressure cooking it settled to the bottom of the jar I did mix it well. Wonder if it was crystalized in the bottle. Has this ever happened to you.
@@7H3M4DH8R Yes this is very common due to over cooking. You should start over, It has to be done very precise. Use very warm but not boiling water and dissolve 1 tablespoon. Pressure cook 30 mins and not a second longer. Try not to go over 15 psi as well.
@@SporenSprout Funny i did 4oz with 1 teaspoon for 10 minutes. My honey was probably just old. I'll try again with new honey.
I have two quart jars, one with blue oyster and one with lions mane. The blue oyster mycelium is growing every day and after two weeks, has filled up nearly half of the liquid medium. The lions mane, however, grew a little in the beginning, but seems to have stalled out in the last week or so.
Is this an issue with the mycelium or could this just be a species specific issue?
What if I would use a liquid culture from a variety that doesn't procedure spores like enigma or penis envy? Could I still do this? This is really nice!
Yes, alternatively you can just throw a piece of colonized agar into the liquid media and it will work the same.
Can you use syringes full of spores to inject into the jar and create liquid culture that way?
@@SporenSprout final question. If I took and cut a small piece from the bottom of a stem and put in the jar. Would that make liquid culture as well?
@@JasonAlexzander1q47 Yes it’s called a needle biopsy, it should be a tiny piece from the very middle of the upper stem. The tissue in the middle is sterile. I find it more reliable to put that piece of sterile tissue on to an agar plate to grow out and ensure it is clean, then a take a slice from the colonized agar plate and put that into liquid culture to expand.
@@SporenSprout 👍
So can I make this liquid culture and then transfer to agar plates?
Yes
can i just use raw honey or cane sugar?
can these be frozen or how do we store them?
can we just use shake it vigorously instead of using a stone?
if i buy new jars and do this, is cooking them really necessary?
thanks :)
Ty for video!
《☆•Danke für den guten Beitrag•☆》
Thanks for watching! This was the first video I ever made. Check out my newer posts!
Will this work for any species of mushroom?
Awesome!
Awesome thank you sir, random question Is growing gray dove Oyster mushrooms the same process as growing lion's mane mushrooms?
Yes I would grow them in the same environment
@@SydThaRager yes