How To Turn 1cc Of Liquid Culture Syringe Into Unlimited Spawn.
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- เผยแพร่เมื่อ 29 ก.พ. 2024
- This video teaches you turn 1cc of liquid culture OR spore syringe into unlimited amounts of spawn. Cultures can be stored for up to 12 months this way before its time for a refresh of stock.
You will learn how to store, manage and maintain a liquid culture syringe properly at home.
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Further reading on the topic can be found here:
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Maintaining LC Syringes, some extra tips in here.
ezmushroom.com/grow/how-to-ma...
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LME Liquid culture recipe.
ezmushroom.com/grow/light-mal...
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LME agar recipe.
ezmushroom.com/grow/light-mal....
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Well done, man. Keeping everything as clean as possible is always a good thing. Mush love!
So i get this, master syringe > agar plates > ( grain jars / liquid culture), liquid culture > grain jars. then repeat process to make more liquid culture. So few questions, why not directly master syringe to liquid culture skipping agar plates ? also how do you refresh the master syringe ? also wanted to appreciate the video, always thought flow hoods are must to attain perfection, glad to see it can be achieved using SAB.
You get to test your syringe for contamination on agar and will know pretty immediately if you failed (within a weekish). If you see contamination you can transfer clean Myc and clean it up.
And because you are using a SAB its a good idea to confirm you have a clean culture before going to liquid culture and you start questioning sterile technique instead of a dud syringe or culture.
For refreshing, move it to agar then a new LC nutrient source and see if it refreshes. However best thing to do is take a piece of myc and put it in longterm storage on agar / distilled water, sealed. I haven't got a video on this currently but search Edward Grands video as he discusses it and so do others.
Tobh LC can still be contaminated even after a successful agar run. You could have likely just got the bit of LC out that wasn't affected. I mean you could just save agar slants just as long so idk. Everyone has a method that works and I say use it.
Where did you purchase your still air box ? Great videos I’ve just came across your channel and I have subbed. Thanks for your time and effort 👍🏻
We sell them on our store, however we are out of stock right now, you can get notified when back in stock
ezmushroom.com/shop/mycology-equipment/still-air-box-mycology-mushroom-cultivation/
NorthSpore have a similar product, and there are some on amazon.
Nice video. Is that a Feather #7 scalpel handle? I wish I could buy those here :) I have to use the shitty Hilbro ones. LMFAO
Indeed it is, feels great to work with, slim.
I've been watching your videos on serial dilution all weekend man so its great to hear from you, big fan haha!
Planning on using it for cordyceps
Check out the 'grab and drag' stuff. Way easier. I haven't done a serial dilution in over a year. @@ezmushroom
Did you have any excess water when cooling down the agar ?
Yes there will be excess inside the container but this clears up after a few days.
Do you have case of contamination throughout the whole process? Even if you are vert precotionous i wanna Know if contamination is common even if im very careful with sterility
I didnt get any contamination. If you follow all the steps in this video you should be fine. If anything goes wrong just try again until you get it right its daunting at first but gets easier as with anything.👍
Do you have a link for those reusable agar pots.
I just picked those up from Asda in the UK.
Thanks, i picked up similar from Aldi, the pots and lids were polyethylene and not polypropylene, they were crushed by the sterilisation process. Lesson learned.
@@ianwynne5483 you can use condiments cups (ketchup cups) from the local box stores just pour them in the SAB. They work fine and maybe have 3 out of 40 or so get contaminated. (Only once was there three usually none when I'm sterile)
Where do you buy the jars?
I don't buy Jars. Buy Beetroot, Pickled food, Saukraut, etc. I've collected most my Jars over years. The lids are often interchangeable and if they break they were going in the bin anyway so nothing is lost.
@@ezmushroom Thank you! Can I use a couple of drops into the agar, once the agar is colonized, could I do agar to agar?
@@goazu Yes definitely.
I'm surprised that heat sterilizing the needle doesn't kill the mycelium inside it.
I would assume the spores inside the metal needle would cook but all the other spores in the syringe should be fine
This is correct^
Only a tiny amount will be inside the needle which gets pushed out