Hi, thanks for the video. Please, I have a question: I will like to ask a question about cell treatment experiment with a test drug for 72 h. In such an experiment, do I have to add my drug once and incubate for 72h then check the cell viability or I have to remove the drug solution and replace with the same drug and concentration after each 24h to avoid wrong readings due to degraded drug in wells or evaporation? Please any other person reading the comments can also reply me if you have the correct answer to my question. Thank you
The usual strategy is to leave the drug on for the entire 72hrs and assess following the designated time. However, it depends on the stability of the drug that you are assessing. If you know that your drug of interest is unstable and is completely degraded after say, 24hrs, then it may be wise to replenish.
![ไลฟ์สุ่ม iPhone 295 บาท... โกงมั้ย?[ โกงมั้ยครับ ep.97 ] | DOM](http://i.ytimg.com/vi/gdstHEE4UUE/mqdefault.jpg)
Cell Viability Assay Protocols: www.ncbi.nlm.nih.gov/books/NBK144065/
Hi, thanks for the video. Please, I have a question: I will like to ask a question about cell treatment experiment with a test drug for 72 h. In such an experiment, do I have to add my drug once and incubate for 72h then check the cell viability or I have to remove the drug solution and replace with the same drug and concentration after each 24h to avoid wrong readings due to degraded drug in wells or evaporation? Please any other person reading the comments can also reply me if you have the correct answer to my question. Thank you
The usual strategy is to leave the drug on for the entire 72hrs and assess following the designated time. However, it depends on the stability of the drug that you are assessing. If you know that your drug of interest is unstable and is completely degraded after say, 24hrs, then it may be wise to replenish.
Thank you very much for your timely reply. The information will add to my considerations and subsequent decision @Adwoabiotech