Next Generation Sequencing 1: Overview - Eric Chow (UCSF)

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  • เผยแพร่เมื่อ 17 ธ.ค. 2024

ความคิดเห็น • 223

  • @hansvanlennep6376
    @hansvanlennep6376 5 ปีที่แล้ว +280

    Finally! Someone that clearly explains the basics of NGS in a understandable way. Thank you very much!

  • @anonviewerciv
    @anonviewerciv 4 ปีที่แล้ว +71

    7:47 Illumina.
    21:00 Nanopore.
    23:23 Pacific Bioscience.

  • @mayureshvishwanathan6458
    @mayureshvishwanathan6458 ปีที่แล้ว +3

    I was hunting for a start to understand basic Next gen sequencing in a easy way and finally got to watch this video. Thank you very much

  • @journeymansix
    @journeymansix 2 ปีที่แล้ว +5

    This is the most lucid explanation of next gen sequencing I've seen. Thanks, Eric!

  • @Lenz979
    @Lenz979 5 ปีที่แล้ว +26

    Eric Chow! Great video. Never seen anyone explaining NGS so well as you do. Impressive. Thank you for your efforts! Please keep on making these videos!

  • @robelbelay4065
    @robelbelay4065 5 ปีที่แล้ว +43

    Absolutely fantastic presentation! Very clear and concise. Thank you

  • @antonioaugustolimateixeirajuni
    @antonioaugustolimateixeirajuni ปีที่แล้ว +2

    I loved this video and how you clearly explained all the basic concepts about NGS

  • @jixingliu9501
    @jixingliu9501 ปีที่แล้ว +2

    Great video! I have messed up details of NGS for many years until seeing this video.

  • @ThePoptartjunkie
    @ThePoptartjunkie 4 ปีที่แล้ว +8

    Fantastic presentation. Its always great to see someone keeping it simple, but simplicity goes a long way. You have educated so many people!

  • @marchielumsy1673
    @marchielumsy1673 3 ปีที่แล้ว +1

    This is such a clear explanation of how these sequencing technologies work. I was focused the whole time. Great work!

  • @danielzarate64
    @danielzarate64 2 ปีที่แล้ว +1

    Thank you!!! That was by far the best breakdown of how NGS works!

  • @inshaishrat4371
    @inshaishrat4371 ปีที่แล้ว +2

    The best video on NGS ever!

  • @rahulboh
    @rahulboh 5 ปีที่แล้ว +8

    So comprehensive and simple to understand, what a great resource. Thank you and Kudos Dr. Chow!

  • @ParisaAarabi-b4f
    @ParisaAarabi-b4f 3 ปีที่แล้ว +3

    Your presentation is MARVELOUS! Thank you so much for explaining it simple enough to allow me to walk through it with my high school knowledge!

  • @rebelScience
    @rebelScience 5 ปีที่แล้ว +79

    Thanks you so much for this! I am a professional programmer, transferring my skills to Bioinformatics on my own. This information is phenomenal. Please do more presentations in this manner.

    • @emekudoh5650
      @emekudoh5650 7 หลายเดือนก่อน

      Would love to have a connect if you don't mind. Thanks

  • @lbgrindel
    @lbgrindel 2 ปีที่แล้ว +1

    Great video. I liked the explanation of alternatives to Illumina. Never knew they existed.

  • @mitalipandey3799
    @mitalipandey3799 4 ปีที่แล้ว +1

    This is a very well thought through and presented video. All the concepts have been researched and presented in a very palatable manner with no frills (background music).The speaker also has very clear diction and doesn't make any unnecessary movements. This allows the viewer to actually see what is being referred to instead of the speaker covering it up. Please keep making and posting these videos - they are very well made and informative. All the best.

  • @CharlesChoMD
    @CharlesChoMD 3 ปีที่แล้ว +4

    Great overview lecture. Thank you so much

    • @toms7695
      @toms7695 3 ปีที่แล้ว

      Even our most educated Doctors have no weak concepts and low knowledge of DNA sequencing ! what an alarming time in medecine when we base our health on mathematics !

  • @flaviorump607
    @flaviorump607 4 ปีที่แล้ว +12

    Thank you, Eric and iBiology team for a very well-produced and thorough video. My exam prep just became more fun :)

  • @SZM396
    @SZM396 ปีที่แล้ว

    Highly recommended to watch. very clear and detailed. thank you for your efforts.

  • @liamnicholas97
    @liamnicholas97 ปีที่แล้ว +1

    What an excellent lecture -Thank you Professor.

  • @SnappyScience
    @SnappyScience 2 ปีที่แล้ว +1

    This was exactly what I needed. A clear explanation of NGS, how it works and why it's important. Thank you!

  • @melix196
    @melix196 3 ปีที่แล้ว +3

    No textbook nor lecturer explained this better. Thank you so much for this high quality video and the recordings from the lab made this video extra interesting ☺️

  • @sdsa007
    @sdsa007 2 ปีที่แล้ว

    Thanks ... I know I watched this before... but its so clear and basic that I watched it again! Thanks for the refresh!

  • @PelleSenior
    @PelleSenior 3 ปีที่แล้ว +1

    Nice job. This was very understandable, yet thorough enough to get a sense for the caveats of different methods.

  • @wanderingmanz
    @wanderingmanz 3 ปีที่แล้ว +2

    Very well explained. Truely professional and takes along the students of all background knowledge.

  • @juancarlostantalean6496
    @juancarlostantalean6496 4 ปีที่แล้ว +2

    Thank you very much Eric !!. I am professor and it is so good for be used in class of Molecular Biology about NGS sequencing. Best regards from Ica-Perú, the place of Nazca Lines !!

  • @donthompson1517
    @donthompson1517 3 ปีที่แล้ว +1

    Thank you Dr Chow! Your explanations are very clear.

  • @audairwashdeh9740
    @audairwashdeh9740 2 ปีที่แล้ว +1

    Thank you so much for simplifying the mystery of NGS! A very helpful video.

  • @zhenli2293
    @zhenli2293 4 ปีที่แล้ว +3

    This is great! Professional explanation and smooth and logical flow, which makes it very easy to understand.

  • @Anetpersa
    @Anetpersa 5 ปีที่แล้ว +8

    I fell in love with molecular biology and chemistry again 😍 Thank you for this amazing video.

  • @tm67245
    @tm67245 4 ปีที่แล้ว +1

    Clear and To the point explanation! thank you Dr. Chow

  • @yibincao7924
    @yibincao7924 5 ปีที่แล้ว +7

    Dear Eric, fantastic NGS sequencing intro! Thank you!

  • @valentinarunko67
    @valentinarunko67 4 หลายเดือนก่อน

    Excellent lecture, far more useful than most other videos TH-cam has on the topic of NGS

  • @wenningqin4210
    @wenningqin4210 4 ปีที่แล้ว +4

    Thank you for making NGS so easy to understand! Really appreciate your taking time to make this video and help!

  • @jsri1703
    @jsri1703 4 ปีที่แล้ว +7

    Excellent teaching starting from scratch. I would like to have another tutorial from you about anchor, true sequencing primer and bar coding with math. I also didn't understand how in an open space a single spot have single (copies) amplified DNA segments.

  • @harshitashrivastava8877
    @harshitashrivastava8877 3 ปีที่แล้ว

    Best video on Sequencing till date.
    Thank you 💯

  • @izj-19
    @izj-19 3 ปีที่แล้ว +1

    This was so clearly explained. Review of basics was really helpful. Thank you!

  • @chelseauju7685
    @chelseauju7685 4 หลายเดือนก่อน

    Excellent video. This is the best explanation for NGS and Sequencing in general! Thank you

  • @razanalrawahi2291
    @razanalrawahi2291 3 ปีที่แล้ว +2

    Omgg you just saved my grade!! thank you so much for this amazing work!

  • @anp9929
    @anp9929 3 ปีที่แล้ว +2

    holy shit my man you've done it to the fucking MAX i love you

  • @user-ko9rj8yg4z
    @user-ko9rj8yg4z 4 ปีที่แล้ว

    Such a neat clear and crisp explanation

  • @speak_up_abu
    @speak_up_abu 2 ปีที่แล้ว

    Just precious way of teaching

  • @yeeguan99
    @yeeguan99 2 ปีที่แล้ว

    Thank you for the clear and consice lecture on NGS!

  • @bronaghmcdonnell8644
    @bronaghmcdonnell8644 ปีที่แล้ว

    Absolutely brilliant 🎉 I'll be watching all of prof Chows videos.
    The reference to suppliers and technologies was excellent, the historical context of Sanger Sequencing was really valuable to then appreciate newer methods, and the videos of the pieces of equipment used was so so so helpful to visualise how this happens in a lab, or on the moon. You covered alot with perfect explanation and delivery. Thank you. I've just started working in a genomics lab and this has helped me alot.

  • @carlosp.e.bedson4011
    @carlosp.e.bedson4011 4 ปีที่แล้ว +1

    An amazingly professional and clear presentation. Thank you!

  • @AsfandyarAfridi
    @AsfandyarAfridi 2 ปีที่แล้ว +1

    GOAT video on sequencing

  • @esan120au
    @esan120au 7 หลายเดือนก่อน

    Thanks so much! Even the illumina webinar was not as clear as your presentation! 👏👏👏👏

  • @wynnewijaya158
    @wynnewijaya158 9 หลายเดือนก่อน

    Thank you so much for this, Eric! Very easy to understand.

  • @franciscoagus
    @franciscoagus 3 ปีที่แล้ว

    Congrats mate. You have some serious teaching skills.
    Quite complicated stuff explained very easy and simple.

  • @sagek7949
    @sagek7949 3 ปีที่แล้ว +1

    This was so very helpful. Thank you so much! This is by far the best explanation on NGS!

  • @eliabd7726
    @eliabd7726 2 ปีที่แล้ว

    I appreciate for all of your videos. Please make more videos.

  • @francescalittle
    @francescalittle 4 ปีที่แล้ว +1

    Thanks to you, I FINALLY understand this topic! You explained it so well.

  • @Mrymena
    @Mrymena 2 ปีที่แล้ว +1

    I have a question about the point at 11:00, which states that all the DNA molecules in a cluster are identical because all are copies of the same template strand. How does the system ensure that when the DNA strands are first introduced to the flow cells, you don't get multiple different template strands binding in the same cluster?

    • @joehickey1745
      @joehickey1745 2 ปีที่แล้ว

      I would like to know this aswell

  • @Science.Medicine209
    @Science.Medicine209 5 ปีที่แล้ว +5

    Excellent and comprehensive presentation ..Thank you Doctor...Kindly Do more video like that specially on its Analysis

  • @manoappapillai
    @manoappapillai 3 ปีที่แล้ว

    Super . . very clear presentation. Please do more. Thank you.

  • @snowballandbubbles9379
    @snowballandbubbles9379 3 ปีที่แล้ว

    The Best lecture on NGS! Excellent!

  • @mechmania8450
    @mechmania8450 3 ปีที่แล้ว +1

    I don't know much about organic chemistry but your explanation was really fantastic.

  • @pek972
    @pek972 3 ปีที่แล้ว

    Excellent! To make so clear something that complex! Thank you very much.

  • @ScottRobertsPage
    @ScottRobertsPage 5 ปีที่แล้ว +3

    Very informative video. This helped me understand how sequencing works much better than the videos from Illumina.

  • @skippyleif
    @skippyleif ปีที่แล้ว

    Excellent video! I love how concise and easy to understand this is. I am starting to analyze NGS data and this just made things much easier.

  • @ahmedhaji4416
    @ahmedhaji4416 4 ปีที่แล้ว +1

    wow , this video and this explanation is just amazing , thank you very much .

  • @Rodrigo-yx3sn
    @Rodrigo-yx3sn 4 ปีที่แล้ว

    Really good explanation and comparison between technologies.

  • @rzipper1716
    @rzipper1716 3 ปีที่แล้ว

    This IS the best review of NGS ! Thanks !!

  • @CraftySmurf35
    @CraftySmurf35 4 ปีที่แล้ว +1

    This was a great video. Please make some more NGS videos ! How about a series on microarray ?

  • @Iqertwyegcje
    @Iqertwyegcje 2 ปีที่แล้ว

    Thank you, it helps me a lot to understand sequencing process

  • @fdboucher
    @fdboucher 4 ปีที่แล้ว +1

    Brilliant presentation! THANK YOU Eric!

  • @laworder5158
    @laworder5158 4 ปีที่แล้ว

    Exciting future for NGS. Thanks for this great video.

  • @MrViermeRau
    @MrViermeRau 5 ปีที่แล้ว +3

    Really good video, it made a lot of things clearer, thanks

  • @dabio3392
    @dabio3392 2 ปีที่แล้ว

    Eric, you need to get an award/recognition for the quality of instruction you provided in these video series. It's incredible how you've distilled such a topic into something very understandable.

  • @rinm2564
    @rinm2564 3 ปีที่แล้ว

    Thank you very much. It is the best explanation! Good luck in your work.

  • @psculpta
    @psculpta 4 ปีที่แล้ว +1

    Excellent job! I wish I could take a class from this professor.

  • @matt-g-recovers
    @matt-g-recovers 3 ปีที่แล้ว +1

    This is outstanding!
    Thank you!

  • @katelinklyn8335
    @katelinklyn8335 2 ปีที่แล้ว

    Very interesting and easy to understand. Thank you!

  • @dr.janmejaykpandey4802
    @dr.janmejaykpandey4802 5 ปีที่แล้ว +1

    Awesome (Very Comprehensible). Your Video helped me a great deal in my class on DNA sequencing.

  • @hesnayigit8840
    @hesnayigit8840 3 ปีที่แล้ว

    Thank you very much, this is a great presentation of the systems.

  • @nicolaeionescu-kosa132
    @nicolaeionescu-kosa132 3 ปีที่แล้ว

    Excellent presentation, could understand more things. Thank you

  • @natalyabeschastnova4049
    @natalyabeschastnova4049 5 ปีที่แล้ว +2

    amazing video! very detailed and clearly explained! thank you for making this video and posting it!

  • @negarolfati36
    @negarolfati36 ปีที่แล้ว +1

    10:48 How does this happen? How do the strands bend and make the U shape?

  • @dangthatscool1
    @dangthatscool1 2 ปีที่แล้ว

    Great overview! Thank you.

  • @justg.5874
    @justg.5874 3 ปีที่แล้ว

    Thank you. Used this to prepare for a job interview💛.

  • @kareemjeiroudi1964
    @kareemjeiroudi1964 5 ปีที่แล้ว

    Thanks for this briefly well-put summary on NGS

  • @mly2883
    @mly2883 3 ปีที่แล้ว

    Thank you Eric, well explained.

  • @boheinz6070
    @boheinz6070 5 ปีที่แล้ว +1

    Really great overview on NGS chemistry, systems, and workflow! Thank you!

  • @Kat-lx7qq
    @Kat-lx7qq 2 ปีที่แล้ว

    wow! this was fantastic thank you so much Eric

  • @angelapotemkina_rozenfeld7137
    @angelapotemkina_rozenfeld7137 4 ปีที่แล้ว

    Thank you very much! This is very clear and understandable explanation of NGS

  • @delprice3007
    @delprice3007 4 ปีที่แล้ว

    Outstanding presentation, many thanks.

  • @nikolayanachkov4553
    @nikolayanachkov4553 3 ปีที่แล้ว

    This was such a great video ! Thank you !

  • @alexandrahiller8223
    @alexandrahiller8223 4 ปีที่แล้ว

    Great presentation. Clear and nicely explained. Thank you!

  • @madhusudanm5835
    @madhusudanm5835 4 ปีที่แล้ว

    great and very informative video presentation. amazing explanation.

  • @kertamo6721
    @kertamo6721 2 ปีที่แล้ว

    Truly a great presentation. Thank you :)

  • @alimughal715
    @alimughal715 5 ปีที่แล้ว

    Realy very nice and understandable presentation.

  • @mervatel-deftar8412
    @mervatel-deftar8412 7 หลายเดือนก่อน

    Amazing overview Excellent

  • @ainawatif1091
    @ainawatif1091 4 ปีที่แล้ว

    it so nice. i could understand much better. Do pray for my master journey !

  • @gokuln9159
    @gokuln9159 2 ปีที่แล้ว

    Awesome session 🎉 Thanks

  • @patrickpetersen9887
    @patrickpetersen9887 4 ปีที่แล้ว

    Very clear and informative...thank you!

  • @CodeJeffo
    @CodeJeffo 2 ปีที่แล้ว

    Excellent explanations. Thank you.

  • @shih-yihsiung239
    @shih-yihsiung239 3 ปีที่แล้ว

    Thanks for uploading this nice video.

  • @aqsasaeed5237
    @aqsasaeed5237 5 ปีที่แล้ว

    Excellent Explanation of the topic

  • @sohamparikh3085
    @sohamparikh3085 4 ปีที่แล้ว

    Very easy to understand, thank you!