Next Generation Sequencing 1: Overview - Eric Chow (UCSF)

www.ibiology.org/techniques/n...
Next generation sequencing allows DNA samples to be sequenced quickly and affordably. Learn how next gen sequencing works and get tips on preparing and running your samples.
In the past decade there has been an amazing change in the efficiency of DNA sequencing. Using traditional Sanger sequencing, the human genome project took 20 years and cost $3 billion. Current next generation sequencing methods allow a human genome to be sequenced for $1000, in 48 hours! In this talk, Eric Chow explains the chemistry behind next generation sequencing, and describes how the next gen sequencers detect and display results. The most commonly used Illumina sequencers are image based and detect the addition of fluorescently labelled nucleotides. Chow also describes two different next generation sequencing technologies which provide benefits such as much longer reads but with downsides such as higher error rates. Chow finishes the talk with some insights into medical applications of next gen sequencing such as much less invasive prenatal testing or cancer detection.
In two short how-to videos, Chow gives advice on purifying DNA samples using magnetic beads and on determining the quality of your nucleic acid sample using an Agilent Bioanalyzer.
Speaker Biography:
Eric Chow is an assistant professor in the Department of Biochemistry and Biophysics and the Director of the Center for Advanced Technology (CAT) at the University of California, San Francisco. The CAT provides resources for UCSF labs wishing to use next generation sequencing techniques and Chow’s research program strives to develop new applications for NGS in pathogen diagnostics. Chow received his BA in molecular biology from the University of California, Berkeley and his PhD in biochemistry from UCSF.

[@hansvanlennep6376]

Finally! Someone that clearly explains the basics of NGS in a understandable way. Thank you very much!

[@rehabalsaleh166]

Exactly!

[@hamidkiangaikani]

Yeah, really!

[@kitony]

Nice one for sure

[@anonviewerciv]

7:47 Illumina.
21:00 Nanopore.
23:23 Pacific Bioscience.

[@rebelScience]

Thanks you so much for this! I am a professional programmer, transferring my skills to Bioinformatics on my own. This information is phenomenal. Please do more presentations in this manner.

[@emekudoh5650]

Would love to have a connect if you don't mind. Thanks

[@robelbelay4065]

Absolutely fantastic presentation! Very clear and concise. Thank you

[@wenningqin4210]

Thank you for making NGS so easy to understand! Really appreciate your taking time to make this video and help!

[@Lenz979]

Eric Chow! Great video. Never seen anyone explaining NGS so well as you do. Impressive. Thank you for your efforts! Please keep on making these videos!

[@yibincao7924]

Dear Eric, fantastic NGS sequencing intro! Thank you!

[@ThePoptartjunkie]

Fantastic presentation. Its always great to see someone keeping it simple, but simplicity goes a long way. You have educated so many people!

[@rahulboh]

So comprehensive and simple to understand, what a great resource. Thank you and Kudos Dr. Chow!

[@flaviorump607]

Thank you, Eric and iBiology team for a very well-produced and thorough video. My exam prep just became more fun :)

[@zhenli2293]

This is great! Professional explanation and smooth and logical flow, which makes it very easy to understand.

[@Anetpersa]

I fell in love with molecular biology and chemistry again 😍 Thank you for this amazing video.

[@user-gr4fh9el3y]

Your presentation is MARVELOUS! Thank you so much for explaining it simple enough to allow me to walk through it with my high school knowledge!

[@marchielumsy1673]

This is such a clear explanation of how these sequencing technologies work. I was focused the whole time. Great work!

[@journeymansix]

This is the most lucid explanation of next gen sequencing I've seen. Thanks, Eric!

[@SnappyScience]

This was exactly what I needed. A clear explanation of NGS, how it works and why it's important. Thank you!

[@bronaghmcdonnell8644]

Absolutely brilliant 🎉 I'll be watching all of prof Chows videos.
The reference to suppliers and technologies was excellent, the historical context of Sanger Sequencing was really valuable to then appreciate newer methods, and the videos of the pieces of equipment used was so so so helpful to visualise how this happens in a lab, or on the moon. You covered alot with perfect explanation and delivery. Thank you. I've just started working in a genomics lab and this has helped me alot.

[@mitalipandey3799]

This is a very well thought through and presented video. All the concepts have been researched and presented in a very palatable manner with no frills (background music).The speaker also has very clear diction and doesn't make any unnecessary movements. This allows the viewer to actually see what is being referred to instead of the speaker covering it up. Please keep making and posting these videos - they are very well made and informative. All the best.

[@melix196]

No textbook nor lecturer explained this better. Thank you so much for this high quality video and the recordings from the lab made this video extra interesting ☺️

[@carlosp.e.bedson4011]

An amazingly professional and clear presentation. Thank you!

[@sagek7949]

This was so very helpful. Thank you so much! This is by far the best explanation on NGS!

[@mayureshvishwanathan6458]

I was hunting for a start to understand basic Next gen sequencing in a easy way and finally got to watch this video. Thank you very much

[@PelleSenior]

Nice job. This was very understandable, yet thorough enough to get a sense for the caveats of different methods.

[@izj-19]

This was so clearly explained. Review of basics was really helpful. Thank you!

[@Science.Medicine209]

Excellent and comprehensive presentation ..Thank you Doctor...Kindly Do more video like that specially on its Analysis

[@natalyabeschastnova4049]

amazing video! very detailed and clearly explained! thank you for making this video and posting it!

[@antonioaugustolimateixeirajuni]

I loved this video and how you clearly explained all the basic concepts about NGS

[@jixingliu9501]

Great video! I have messed up details of NGS for many years until seeing this video.

[@SZM396]

Highly recommended to watch. very clear and detailed. thank you for your efforts.

[@donthompson1517]

Thank you Dr Chow! Your explanations are very clear.

[@danielzarate64]

Thank you!!! That was by far the best breakdown of how NGS works!

[@inshaishrat4371]

The best video on NGS ever!

[@francescalittle]

Thanks to you, I FINALLY understand this topic! You explained it so well.

[@audairwashdeh9740]

Thank you so much for simplifying the mystery of NGS! A very helpful video.

[@tm67245]

Clear and To the point explanation! thank you Dr. Chow

[@skippyleif]

Excellent video! I love how concise and easy to understand this is. I am starting to analyze NGS data and this just made things much easier.

[@fdboucher]

Brilliant presentation! THANK YOU Eric!

[@wanderingmanz]

Very well explained. Truely professional and takes along the students of all background knowledge.

[@harshitashrivastava8877]

Best video on Sequencing till date.
Thank you 💯

[@wynnewijaya158]

Thank you so much for this, Eric! Very easy to understand.

[@user-ko9rj8yg4z]

Such a neat clear and crisp explanation

[@franciscoagus]

Congrats mate. You have some serious teaching skills.
Quite complicated stuff explained very easy and simple.

[@snowballandbubbles9379]

The Best lecture on NGS! Excellent!

[@razanalrawahi2291]

Omgg you just saved my grade!! thank you so much for this amazing work!

[@alexandrahiller8223]

Great presentation. Clear and nicely explained. Thank you!

[@sdsa007]

Thanks ... I know I watched this before... but its so clear and basic that I watched it again! Thanks for the refresh!

[@dr.janmejaykpandey4802]

Awesome (Very Comprehensible). Your Video helped me a great deal in my class on DNA sequencing.

[@kareemjeiroudi1964]

Thanks for this briefly well-put summary on NGS

[@jishasureshkumar2466]

Loved this presentation! Clearly explained.

[@MrViermeRau]

Really good video, it made a lot of things clearer, thanks

[@eliabd7726]

I appreciate for all of your videos. Please make more videos.

[@juancarlostantalean6496]

Thank you very much Eric !!. I am professor and it is so good for be used in class of Molecular Biology about NGS sequencing. Best regards from Ica-Perú, the place of Nazca Lines !!

[@exciton007]

Thanks for this great presentation!

[@liamnicholas97]

What an excellent lecture -Thank you Professor.

[@Gyutaro602]

Thank you for the clear and consice lecture on NGS!

[@lbgrindel]

Great video. I liked the explanation of alternatives to Illumina. Never knew they existed.

[@mattgraves3709]

This is outstanding!
Thank you!

[@abutaleb3622]

Just precious way of teaching

[@rinm2564]

Thank you very much. It is the best explanation! Good luck in your work.

[@sangeethasaji1]

Its a fantastic explanation. Great work

[@ScottRobertsPage]

Very informative video. This helped me understand how sequencing works much better than the videos from Illumina.

[@madhusudanm5835]

great and very informative video presentation. amazing explanation.

[@Rodrigo-yx3sn]

Really good explanation and comparison between technologies.

[@delprice3007]

Outstanding presentation, many thanks.

[@mly2883]

Thank you Eric, well explained.

[@rzipper1716]

This IS the best review of NGS ! Thanks !!

[@andrewrobinson5581]

Thank you so much this presentation was perfect!

[@nikolayanachkov4553]

This was such a great video ! Thank you !

[@pek972]

Excellent! To make so clear something that complex! Thank you very much.

[@angelapotemkina_rozenfeld7137]

Thank you very much! This is very clear and understandable explanation of NGS

[@justg.5874]

Thank you. Used this to prepare for a job interview💛.

[@laworder5158]

Exciting future for NGS. Thanks for this great video.

[@anp9929]

holy shit my man you've done it to the fucking MAX i love you

[@jsri1703]

Excellent teaching starting from scratch. I would like to have another tutorial from you about anchor, true sequencing primer and bar coding with math. I also didn't understand how in an open space a single spot have single (copies) amplified DNA segments.

[@manoappapillai]

Super . . very clear presentation. Please do more. Thank you.

[@AsfandyarAfridi]

GOAT video on sequencing

[@hesnayigit8840]

Thank you very much, this is a great presentation of the systems.

[@FufuFang]

Thank you so much!!! This is really informative!

[@psculpta]

Excellent job! I wish I could take a class from this professor.

[@aqsasaeed5237]

Excellent Explanation of the topic

[@sawses]

Thanks for this! I'm a recent bio grad and wanted to figure out how NGS differs from Sanger. Well done.

[@nguyetnhiau1]

Great presentation! Thank you so much!

[@shih-yihsiung239]

Thanks for uploading this nice video.

[@kertamo6721]

Truly a great presentation. Thank you :)

[@irenenatal1]

Thank you, it helps me a lot to understand sequencing process

[@patrickpetersen9887]

Very clear and informative...thank you!

[@mervatel-deftar8412]

Amazing overview Excellent

[@hangpham7588]

Thank you so much! This's all I need

[@matiwosaraya514]

Great video, Thanks for your time.

[@Stephanbitterwolf]

Thank you for this great lecture

[@mayeninim6281]

This is great! Thank you!

[@katelinklyn8335]

Very interesting and easy to understand. Thank you!

[@CodeJeffo]

Excellent explanations. Thank you.

[@mechmania8450]

I don't know much about organic chemistry but your explanation was really fantastic.

[@karlalima6497]

Thanks you so much for this!

[@ekimkara9260]

Hi, thank you for this review!

[@alimughal715]

Realy very nice and understandable presentation.

[@nurain6372]

Thank you for this video

[@gokuln9159]

Awesome session 🎉 Thanks

[@nicolaeionescu-kosa132]

Excellent presentation, could understand more things. Thank you