Blood Bank- Full Crossmatch (XM)

In this video I show you how to preform a full Crossmatch using a Low ionic strength solution (LISS). In the video I check for agglutination at 3 stages: immediate spin, 37 degrees Celsius, and AHG. Finally, I check my work using check cells.

[@biosphere7762]

Today in the morning I had did this procedure it was easy And effective thank you 😊

[@Jelly-rj8fz]

I wrote a thesis on low ionic strength solution crossmatch method..long time ago now, forgotten most of it!! the favourite part of it for me was microscopes (much more interesting than a mirror!) and also the antibody panel for discovering which antibodies patients had that were causing incompatibility... I LOVE puzzles.

[@labvideossa1667]

I'm from Saudi Arabia Thank you Robert

[@the.mermaid.scientist]

1:57 Hey that's the timer for my coag bench! I was wondering where it went!! LOL! 🤣😅

[@empathlove5576]

Very helpful ty!

[@fantastic9826]

Thank you very much!👏👏👏👏👏👏👏👏

[@bladeandsoul4128]

I'm gonna ask a question. Uhmm, aren't you supposed to wash the donor red cells for 3x before making a cell suspension?

[@robertbounds8112]

I've never washed donor cells when making a cell suspension. However, for a full crossmatch, you need to wash them before adding AHG (preferably 3x).

[@samo716]

Thank you

[@robertbounds8112]

You’re welcome

[@basit1202]

Good

[@krmanjahmad659]

excuse me what was that equipment that you used for the segmente please?

[@Jeagerjaques]

check cell is QC the previous steps?

[@robertbounds8112]

Check cells ensure that the cells were properly washed. Failure to properly wash the the cells will result in the AHG binding to unbound serum proteins (resulting in zero agglutination with the check cells).

[@labvideossa1667]

Why not use a microscope to detect red blood cells associated with some

[@robertbounds8112]

Do you mean checking microscopically for agglutination? I do in some instances but not typically.

[@drnooronline3273]

Good