Crisp and clear ... Could you provide the rationale for the each and every step why it is done from the next video ( atleast ) so that we need not read it multiple times. Thanks for such an amazing video.I'm a vet too 🙂.
as part of my work i regularly run indirect coombs tests for pregnant women Rh+ ab screening. i have never seen anything close to the hemagglutinations you showed. but i rarely see only one small agglutination on the whole slide and according to my education, that's not enough to call it positive. also, i have never seen the technique of mixing the button at the bottom being mixed with a micropipette. instead we shake the tube while examining it under the light. if it's macroscopically negative, then we put lamellas (slide cover slips) over it so as to avoid roleaux formation.
Can I use plasma for BUN, Gaba, NPY, CCK, SOD, GSH-PX deterimnation in dairy goats. I have read papers where serum is used but my advisor saying to use plasma, please advise.
Major is Donor blood and recipient serum, ( helps to know if recipient has pre existing antibodies against donor blood type) Minor is recipient blood and donor serum ( here we ll know if donor has pre existing antibodies or complements are present against recipient blood ). Both should be not have agglutination
Annajarvis Pao If you want to differentiate rouleaux from agglutination then add a drop of normal saline If erythrocytes seperate then it is rouleaux If they still appear clumped then it is agglutination. Agglutination showed there are bound antibodies on the erythrocytes surface.
Hey! I wanted to ask you about human blood bank system in India if you any idea.. so in India after ABO and RhD typing of the patient blood do we do antibody screening of we directly go for cross matching?
Nice video, I just got confused how we can differentiate rouleaux to agglutination, sometime I am doing this test I get so confused if I am really seeing rouleaux
vinicius prado de oliveira Is you see stack of coin like appearance then it is rouleaux. Agglutination is an irregular aggregation of RBCs which will appear like the one I showed in video.
Varsha Scooby and like you said I can also add one more normal saline drop if I still have some doubt? If it is rouleaux it may disappear if it is not rouleaux it will keep toetther right?
Antibodies require an optimal temperature to bind with antigen to form antigen antibody complex. Here we need them to know if blood is compatible or not.
Mam..plz tell me that ...Is major minor test necessary for primary blood transfusion in dogs? Is there any risk of primary blood transfusion without do any major minor test in dogs..?? Plz tell me...it's very urgent for me to know that. ..
Nandani Kaur First time blood transfusion without cross matching is fine if it is emergency But best practise is always do typing or transfuse DEA 1 negative blood. Second transfusions 100% require both cross matching and blood typing test. Because if blood doesn’t match the reaction can be fatal. Better to do properly 😊
If you watch the video fully, I would have incubated 4 tubes, 2 of them are major and minor, other two are donor and recipient controls to check per existing agglutination.
Thank you so much for crystal clear explanation.
Brief explanation of major and minor matching. Thanks.
Crisp and clear ... Could you provide the rationale for the each and every step why it is done from the next video ( atleast ) so that we need not read it multiple times. Thanks for such an amazing video.I'm a vet too 🙂.
Brief explanation of major & minarets matching Thanks you so much
as part of my work i regularly run indirect coombs tests for pregnant women Rh+ ab screening. i have never seen anything close to the hemagglutinations you showed. but i rarely see only one small agglutination on the whole slide and according to my education, that's not enough to call it positive.
also, i have never seen the technique of mixing the button at the bottom being mixed with a micropipette. instead we shake the tube while examining it under the light. if it's macroscopically negative, then we put lamellas (slide cover slips) over it so as to avoid roleaux formation.
A very nice video . But i think we have to test for ABO blood group first.
Very nicely explained. Thank you.
Excellent explained.
Thanks a lot 🫡
Nice explanation mam... Thankyou very much
Thank you for such a good description
Just add procedure in this video disruptions..
#@@#@@##!@@ISMAILBASHA_9181
Beautifullly explained 😊
Help full video thanks for this video
Thanks for given useful knowledge mam
Thank you 😊
excellently explained. thanks a lot
thank you so much very nice explained.
I like the video doc...
Any video on blood indices
Great 👍👍... Keep it up
Thank you very useful video
Very nice explanation 👌
Thanks it was wonderful 😊
Thanks for your explain very use full video
thank you so much
Enjoyed watching your video and learned a lot
How many procedures do cross matches do?
Very helpful video thank you
Thanks mam for your these video to my updates knowledge and cleared my doubts.
Prakash Prakash welcome 🙏🏾
Great video, thanks!
Your welcome
Nice video for cross matching
very good we understand you're presence
Thank You..Need it..😊👍
Can I use plasma for BUN, Gaba, NPY, CCK, SOD, GSH-PX deterimnation in dairy goats. I have read papers where serum is used but my advisor saying to use plasma, please advise.
Amazing Explanation...👌👌👌
praveen kumar Thankyou!
Mai laboratory technologist hu mujhe ek cheej badi psnd aaae aapki....o hai last wali smile....👏
Please mention the reference book from which you have made this video.
Kirk and bistners handbook of veterinary procedures and emergency treatment
Really nice video
Very good & clear presentation
Thankyou sir
After wash RWC, why do you added RBC donor to donor serum?
danh le thanh To rule out auto agglutination in donor blood and also acts as negative control
Very helpful. Thank you.
Welcome
Thanks mam !!!
Thank u very much 😊
Thank you
Good JOB 👍👍🎉🎉
Excellent mam
Thanks very much
Thanks ❤
great explanation. thank u
Welcome 😍
Explain it in AHG and albumin method also
Thanks u mam
A very nice video
Very useful video mam
What happen if I added AHG reagent before microscopic examination??and what the differences between minor,major cross matching?
Major is Donor blood and recipient serum, ( helps to know if recipient has pre existing antibodies against donor blood type)
Minor is recipient blood and donor serum ( here we ll know if donor has pre existing antibodies or complements are present against recipient blood ).
Both should be not have agglutination
Like it ❤️
Give more video on microbiology topics.
Can you tell me what topics should I cover?
really helpful for me.
im a science teacher.
Your presentation is quite gentil and professional i like it
Wishing you a very happy new year 2 o 2 3 with family dear madam
Thank you very much ❤️❤️❤️
Very nicely explained, thank you!
BTW, is it rouleaux formation normal in dogs?
Annajarvis Pao
If our canine patient has high proteins / acute phase proteins / on going inflammation then rouleaux formation is profoundly seen
Annajarvis Pao
If you want to differentiate rouleaux from agglutination then add a drop of normal saline
If erythrocytes seperate then it is rouleaux
If they still appear clumped then it is agglutination.
Agglutination showed there are bound antibodies on the erythrocytes surface.
Doc, what about in equine?
Annajarvis Pao For equines use Alvedia blood typing and cross matching kits.
Nice video
Its my humble request to make a video on different blood groups in different species of animal's.
Excellent suggestion
Thank you so much madam
Why is the rinsing step necessary? Can results be trusted if the rinsing steps are skipped?
Sabrina Covey Can’t be
Washing RBcs is very very important
Without washing RBCs you will see unnecessary clumping that will interfere with true agglutination
Thanku mam
Great information
Thankyou
described very clearly
Thankyou
thanks alot
Please send tail cross matching procedures
Tq ❤️
This is such a great video, thank you very much.
Thankyou! Welcome 😊😊😊
Come back pls
Hi akka Thanks for information akka
thanks mam
Thank u mam
Hey! I wanted to ask you about human blood bank system in India if you any idea.. so in India after ABO and RhD typing of the patient blood do we do antibody screening of we directly go for cross matching?
They do both actually
@@Pathologymadesimple oh i see! Thank you😀
Mam plz make cross matching videos with use of jel card
Goo job
Nice video, I just got confused how we can differentiate rouleaux to agglutination, sometime I am doing this test I get so confused if I am really seeing rouleaux
vinicius prado de oliveira Is you see stack of coin like appearance then it is rouleaux. Agglutination is an irregular aggregation of RBCs which will appear like the one I showed in video.
Varsha Scooby and like you said I can also add one more normal saline drop if I still have some doubt? If it is rouleaux it may disappear if it is not rouleaux it will keep toetther right?
vinicius prado de oliveira exactly
nice
In this video not running ordering, missing some clips
Nyc
Nice
Nice 👍
EDTA vial me serum kha se aayga vo to red vial m ayga n
Edta vial - plasma
Did I say it wrongly?
👌🏻👍🏻
good
Your video is good.
BHUSHAN KUMAR thankyou so much
So nice your testing method majar n minor
why do we incubate and refrigerate?
Antibodies require an optimal temperature to bind with antigen to form antigen antibody complex. Here we need them to know if blood is compatible or not.
@@Pathologymadesimple thank you for the prompt reply.
It is plasma or serum?🙄
Serum
👌
Thanks for this.
I couldn't hear what she said at 1:33 clearly though.
I couldn't hear what she said at 3:07 too.
How to do Crossmatch of neonate
Same proceudre
Super
200 Serum + 200cell suspension tell please
200 micro litre serum and 200 micro litre washed RBCs
Superbbbbbbbbbbbb darling
Tq youuuuuuuuuuu for loveing my coment
Good night swt dream
You are very beautiful 😍 last seen bye
give more video on microbiology topics mam
Pooja chaudhary Sure I will do that. 👍🏽
tnk u mam
How many hours in cross matching
It takes 1 hour only to cross match
@@Pathologymadesimple Thanks mam I need job mam your.lab available job
Where is ur lab mam in State
When you said microliter, did you actually mean milliliter?
ml
Microliter 😀
Mam topics on immunology.
Ashish Verma I have done a very few on clinical immune hematology
Topics such as auto agglutination test
DAT Coombs test etc
Thanks mam
Mam..plz tell me that ...Is major minor test necessary for primary blood transfusion in dogs? Is there any risk of primary blood transfusion without do any major minor test in dogs..??
Plz tell me...it's very urgent for me to know that. ..
Nandani Kaur
First time blood transfusion without cross matching is fine if it is emergency
But best practise is always do typing or transfuse DEA 1 negative blood.
Second transfusions 100% require both cross matching and blood typing test. Because if blood doesn’t match the reaction can be fatal.
Better to do properly 😊
very Nice
Nice video
normal saline and rbc 3 times washing how much centrifuge rpm
Donor RBC with recipient serum. As well as Recipient RBC with donor serum But u show Donor RBC with donor serum,???????
That is donor and recipient control, to check autoagglutination.
If you watch the video fully, I would have incubated 4 tubes, 2 of them are major and minor, other two are donor and recipient controls to check per existing agglutination.