Best one I've seen so far. Thanks for showing the tube labeling and grading of agglutinations. We were all confused the first time. So this really helps. I can't thank you enough. ;)
I would love to see you remake this video by putting all the clear stuff in first, including the patient's plasma in reverse tubes, then add the patient and reagent red cells so that way the students won't forget to add it. Hard to tell once covered by red cells, trying to avoid ABO discrepancy
I suppose I do it that as a matter of habit. I usually set up a tube ABORh and gel card with all reagents added (I do this whilst the pink top is spinning). Then I add patient plasma to the gel card and reverse type followed by making a 3% cell suspension for the forward type. I don’t separate the plasma before hand so it flows best this way.
Hi Robert! Thank you so much for this useful video. I have a (perhaps silly) question. Why can't the reverse reaction be done with whole blood, not just serum? Is it that that the antibodies aren't concentrated enough in whole blood and the reaction wouldn't be very visible?
I suppose it would still produce an agglutination but it would be mixed field due to the presence of the patients cells which could be problematic. A forward type alone would be okay with whole blood (if you lacked the ability to centrifuge). However, if a cold agglutinin was present your forward type could be erroneous. It’s important to perform a negative control (e.g. saline and patient cells) when the entire forward type is positive (I.e. AB positive patient) to avoid such a mistype.
Great video thnx so much. Can u pllzzz make a video on how u can work out which group it is. My teacher explained it i didn't get her. She said something abt 'the 3 rules control, oppositr reaction strong reaction' i didn't get this plz explain
+Jessie Molly HI, I added some videos that might help you. Was your teacher referring to antibody rule outs using 3 cells and homozygous and heterozygous cells showing dosage (weaker or stronger agglutinations)?
At my facility we only use anti AB when processing Type O units into our supply (from One Blood and such). We do not use it for our forward and reverse typing.
moody moody you must add 30 micro litre of rbc in 970 micro litre of saline to make 3% suspension Or you can add 15 micro litre of rbc in 485 microlitre of saline .
What this ? So thanks u make it so difficult to me I’m really confused 😐 If the agglutination like this 🔴 consider as positive ? If after mixing disappear is consider as negative or what !!! 🥲🥲 I know it’s easy but WHY is confused me 😭
Best one I've seen so far. Thanks for showing the tube labeling and grading of agglutinations. We were all confused the first time. So this really helps. I can't thank you enough. ;)
+Grace Smith you are very welcome! Thanks for watching! Let me know if there is any other videos you'd like to see.
Thank you, sir, you showed them really easy and simple. I have a blood bank class and tomorrow is our lab test on these things. Thank you
Thank you sir. I have a hematology exam tomorrow. This helped me out big time!
Thank you. This video explained a concept I was having a hard time with.
That was the suspension preparation of a man who has done this far too many times to count.
T
Thank you..
New follower From Saudi Arabia..😇
I would love to see you remake this video by putting all the clear stuff in first, including the patient's plasma in reverse tubes, then add the patient and reagent red cells so that way the students won't forget to add it. Hard to tell once covered by red cells, trying to avoid ABO discrepancy
I suppose I do it that as a matter of habit. I usually set up a tube ABORh and gel card with all reagents added (I do this whilst the pink top is spinning). Then I add patient plasma to the gel card and reverse type followed by making a 3% cell suspension for the forward type. I don’t separate the plasma before hand so it flows best this way.
I have 15 years blood banking experience would love to come work in the USA.❤❤❤
Nice channel, thank you
Great, Sir can u please make a video regarding making of 5% red cell suspension
I love this lab, really fun. It was too bad we didn't use our own blood to do the lab ...
Thanks alot my lecture. Could you make video about blood glucose estimation
So the blood type was O+?
Yeah
I like this lab, so much fun
So after all these test what blood type would this patient be?
Hi Robert! Thank you so much for this useful video. I have a (perhaps silly) question. Why can't the reverse reaction be done with whole blood, not just serum? Is it that that the antibodies aren't concentrated enough in whole blood and the reaction wouldn't be very visible?
I suppose it would still produce an agglutination but it would be mixed field due to the presence of the patients cells which could be problematic. A forward type alone would be okay with whole blood (if you lacked the ability to centrifuge). However, if a cold agglutinin was present your forward type could be erroneous. It’s important to perform a negative control (e.g. saline and patient cells) when the entire forward type is positive (I.e. AB positive patient) to avoid such a mistype.
@@robertbounds8112 thanks a lot!
good job
thank you
Thank you and you’re welcome. 😀
Thank you! :)
You're welcome!
Great video thnx so much. Can u pllzzz make a video on how u can work out which group it is. My teacher explained it i didn't get her. She said something abt 'the 3 rules control, oppositr reaction strong reaction' i didn't get this plz explain
+Jessie Molly HI, I added some videos that might help you. Was your teacher referring to antibody rule outs using 3 cells and homozygous and heterozygous cells showing dosage (weaker or stronger agglutinations)?
@@robertbounds8112 I think she meant the different blood types. Each shown in a aborh
A+, B- etc.
O+
If i want do antibody screening control gel card? Can u tell me how?
What is the purpose of “Reagent red cells A1 and B”?
How long should you spin in the centrifuge ? Thank you..
thanks
+anmar ali You are very welcome. Thanks for watching!
How many drops of RBCs do u pipet into the 3% tube
In forward typing the serum of recipient is used? How about in reverse typing what sample is needed?
forward sample: red cells
reverse sample: plasma
2:00 why two drops?
He might have wanted a stronger reaction. There are situations where you may need to increase the plasma:cell ratio
So what is the result Sir?
you should use serum instead !?
Serum could be used but EDTA plasma is more common where I have worked. We do use serum for neonates.
Where is anti AB?
At my facility we only use anti AB when processing Type O units into our supply (from One Blood and such). We do not use it for our forward and reverse typing.
how to prepare 3% washed Rbcs
moody moody you must add 30 micro litre of rbc in 970 micro litre of saline to make 3% suspension Or you can add 15 micro litre of rbc in 485 microlitre of saline .
0:25 Are you sure is that plasma ?
It's plasma. Although you could use serum too.
Oh ok. Because I've never seen a plasma used for reverse ABO test.
Told in hindi
ممكن بالعربي
I'll translate for you
can you determine the Rh with reverse typing?
You cannot. Only ABO type.
no
What this ? So thanks u make it so difficult to me I’m really confused 😐
If the agglutination like this 🔴 consider as positive ? If after mixing disappear is consider as negative or what !!! 🥲🥲
I know it’s easy but WHY is confused me 😭