Qubit Assay steps 1. Determine number of samples you need to assay 2. Each sample requires 200ul of dye-infused buffer. 3. If dye and buffer is supplied separately, do a 1/200 dilution of the dye into the buffer (e.g. if you have 50 samples, then you require 50 x 200ul = 10,00ul/10ml : dilute the total vol. required by 200 to adjust the dye conc. from 200x to 1x (e.g. 10ml/200 = 0.050ml, 50ul) 5. Decide dilution to use for the sample in order to be in the assay reading range (detectable and not above the assay range). 6. On the instrument, choose the assay type for your read (ds DNA/ss DNA/RNA, broad range/High sensitivity). 7. Indicate the vol. of sample added to the dye-mix on the machine, so that the software can report the conc. as the final conc. (without needing to multiply by the dilution factor).
What's your lab's go-to method for quantifying biomolecules; direct light absorption of the compound or reagent-coupled indirect detection (e.g. fluorescent or other dyes)?
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Qubit Assay steps
1. Determine number of samples you need to assay
2. Each sample requires 200ul of dye-infused buffer.
3. If dye and buffer is supplied separately, do a 1/200 dilution of the dye into the buffer (e.g. if you have 50 samples, then you require 50 x 200ul = 10,00ul/10ml : dilute the total vol. required by 200 to adjust the dye conc. from 200x to 1x (e.g. 10ml/200 = 0.050ml, 50ul)
5. Decide dilution to use for the sample in order to be in the assay reading range (detectable and not above the assay range).
6. On the instrument, choose the assay type for your read (ds DNA/ss DNA/RNA, broad range/High sensitivity).
7. Indicate the vol. of sample added to the dye-mix on the machine, so that the software can report the conc. as the final conc. (without needing to multiply by the dilution factor).
Thank youð
does the volume of the samples have to be constant
Without having more details about which volume you are referring to, I will still say 'yes', because volume affects concentration.
What's your lab's go-to method for quantifying biomolecules; direct light absorption of the compound or reagent-coupled indirect detection (e.g. fluorescent or other dyes)?